Effect of total glucosides of Cistanche deserticola on oxidative stress and cognitive function in rats with intermittent hypoxia_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

 ZHANG Panpan , HAN Xiaoqing , WANG Yuan , HUANG Chao , WANG Hongyang

Department of Respiratory Medicine, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei 063000, P. R. China;

Corresponding?author：

ZHANG Panpan, Email: 1983zhangpanpan@163.com

Keywords：

Intermittent hypoxia; total glycosides of Cistanche deserticola; oxidative stress; cognitive function

DOI：

10.7507/1671-6205.202212001

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Objective To explore the effect of total glucosides of Cistanche deserticola on oxidative stress and cognitive function in rats with intermittent hypoxia. Methods Adult male Wistar rats (n=72) were randomly divided into three groups: a blank control group, a 5% intermittent hypoxia group (IH group) and a total glucosides of Cistanche deserticola intervention group (TGs intervention group). The 5% intermittent hypoxia rat model was simulated by using the self-made cabin of intermittent hypoxia. The rats in the IH group and the TGs intervention group were given 5% intermittent hypoxia respectively, and the rats in the TGs intervention group were treated with total glucosides of Cistanche deserticola simultaneously. Learning and memory function was tested by Morris water maze in three groups at the 2^nd, 4^th, 6^th and 8^th week respectively. The expressions of superoxide dismutase (SOD) and malondialdehyde (MDA) in hippocampus were detected by test kit. Results Compared with the blank control group, the escape latency time of the rats in the IH group and the TGs intervention group was significantly prolonged at the 2^nd, 4^th, 6^th and 8^th week respectively (P<0.05). The time to cross the target quadrant in the IH group and the TGs intervention group was gradually shortened at the 2^nd, 4^th, 6^th and 8^th week respectively (P<0.05). Compared with the IH group, the escape latency gradually shortened at the 2^nd, 4^th, 6^th and 8^th week in the TGs intervention group (P<0.05), while the time to cross the target quadrant was gradually prolonged at the 2^nd, 4^th, 6^th and 8^th week (P<0.05). The expressions of MDA in hippocampal tissue in the IH group and the TGs intervention group increased at the 2^nd, 4^th, 6^th and 8^th week (P<0.05), which were significantly higher than those in the blank control group; and the expressions of SOD at the 2^nd, 4^th, 6^th, and 8^th week were all lower than those in the blank control group(P<0.05). Compared with the IH group, the expression of MDA protein in hippocampal tissue in the TGs intervention group decreased at the 2^nd, 4^th, 6_th and 8^th week, while the expression of SOD protein at the 2^nd, 4^th, 6^th and 8^th week increased, and the differences were statistically significant (P<0.05). Conclusion The total glycosides of Cistanche deserticola can improve the learning and memory function of intermittent hypoxia rats by inhibiting oxidative stress.

Citation： ZHANG Panpan, HAN Xiaoqing, WANG Yuan, HUANG Chao, WANG Hongyang. Effect of total glucosides of Cistanche deserticola on oxidative stress and cognitive function in rats with intermittent hypoxia. Chinese Journal of Respiratory and Critical Care Medicine, 2023, 22(4): 269-273. doi: 10.7507/1671-6205.202212001 Copy

1.	張盼盼, 汪彥輝, 韓曉慶, 等. 丁苯酞對老年OSAHS患者氧化應激及認知功能的影響. 臨床耳鼻咽喉頭頸外科雜志, 2018, 32(18): 1422-1425.
2.	王樂, 張靜, 王彥, 等. 整夜睡眠血氧飽和度低于90%時間占總監測時間的百分比與阻塞性睡眠呼吸暫停低通氣常見合并癥的關系. 中華結核和呼吸雜志, 2020, 43(1): 63-67.
3.	Vanek J, Prasko J, Genzor S, et al. Obstructive sleep apnea, depression and cognitive impairment. Sleep Med, 2020, 72: 50-58.
4.	Xu LH, Yang YB, Chen J. The role of reactive oxygen species in cognitive impairment associated with sleep apnea. Exp Ther Med, 2020, 20(5): 4.
5.	Zhou L, Chen P, Peng YT, et al. Role of oxidative stress in the neurocognitive dysfunction of obstructive sleep apnea syndrome. Oxid Med Cell Longev, 2016, 2016: 9626831.
6.	董文平, 任壽安. 血清核轉錄因子紅系2相關因子2、血紅素加氧酶-1水平與阻塞性睡眠呼吸暫停患者認知障礙的相關性研究. 中國呼吸與危重監護雜志, 2022, 21(7): 478-483.
7.	韓思璐, 馬迎民. 阻塞性睡眠呼吸暫停患者持續氣道正壓通氣治療相關吞氣癥和胃食管反流. 中國呼吸與危重監護雜志, 2018, 17(6): 639-643.
8.	Jia JX, Yan XS, Cai ZP, et al. The effects of phenylethanoid glycosides, derived from Herba cistanche, on cognitive deficits and antioxidant activities in male SAMP8 mice. J Toxicol Environ Health A, 2017, 80(22): 1180-1186.
9.	Yang JH, Ju BW, Hu JP. Effects of phenylethanoid glycosides extracted from Herba cistanches on the learning and memory of the APP/PSI transgenic mice with Alzheimer's disease. Biomed Res Int, 2021, 2021: 1291549.
10.	王璐, 白雨朦, 李曉宇, 等. 肉蓯蓉總苷對阿爾茨海默病模型大鼠學習認知功能和氧化應激的影響. 解剖學雜志, 2020, 43(3): 194-199.
11.	王紅陽, 韓曉慶, 趙雅寧, 等. 重度間歇低氧大鼠學習記憶功能與氧化應激的關系. 中華結核和呼吸雜志, 2012, 35(1): 24-28.
12.	張盼盼, 韓曉慶, 王紅陽, 等. 間歇低氧大鼠神經元特異性烯醇化酶蛋白的表達及其對學習記憶功能的影響. 中華行為醫學與腦科學雜志, 2014, 23(4): 303-306.
13.	Patel A, Chong DJ. Obstructive sleep apnea: cognitive outcomes. Clin Geriatr Med, 2021, 37(3): 457-467.
14.	Tóthová ?, Celec P, Mucska I, et al. Short-term effects of continuous positive airway pressure on oxidative stress in severe sleep apnea. Sleep Breath, 2019, 23(3): 857-863.
15.	馬曉蓉, 張靜宜, 宗運之, 等. 無創呼吸機治療對阻塞性睡眠呼吸暫停低通氣綜合征患者骨代謝和氧化應激影響的研究. 中國呼吸與危重監護雜志, 2022, 21(10): 731-736.
16.	Rosenzweig I, Glasser M, Crum WR, et al. Changes in neurocognitive architecture in patients with obstructive sleep apnea treated with continuous positive airway pressure. EBioMedicine, 2016, 7: 221-229.
17.	韓曉慶, 張盼盼, 李琳, 等. 依達拉奉對重度間歇低氧大鼠認知功能及Nogo-A表達的影響. 中國中西醫結合急救雜志, 2017, 24(6): 570-574.
18.	Abdel-Wahab BA, Abdel-Wahab MM. Protective effect of resveratrol against chronic intermittent hypoxia-induced spatial memory deficits, hippocampaloxidative DNA damage and increased p47Phox NADPH oxidase expression in young rats. Behav Brain Res, 2016, 305: 65-75.
19.	Wang FJ, Li RY, Tu PF, et al. Total glycosides of Cistanche deserticola promote neurological function recovery by inducing neurovascular regeneration via Nrf-2/Keap-1 pathway in MCAO/R rats. Front Pharmacol, 2020, 11: 236.
20.	薛海燕, 焦嬋媛, 姚軍. 肉蓯蓉總苷藥理作用的研究現狀. 中國臨床藥理學雜志, 2018, 34(4): 486-488.
21.	Ling JZ, Yu Q, Li YN, et al. Edaravone improves intermittent hypoxia-induced cognitive impairment and hippocampal damage in rats. Biol Pharm Bull, 2020, 43(8): 1196-1201.
22.	Elliot-Portal E, Laouafa S, Arias-Reyes C, et al. Brain-derived erythropoietin protects from intermittent hypoxia-induced cardiorespiratory dysfunction and oxidative stress in mice. Sleep, 2018, 41(7): zsy072.
23.	樊燕燕, 張石在. 肉蓯蓉總苷通過調控lncRNA GAS5保護神經細胞缺血再灌注損傷. 中成藥, 2022, 44(7): 2320-2324.
24.	劉恩寵, 田原, 韋瑤, 等. 肉蓯蓉毛蕊花苷改善腦低灌注致小鼠認知功能障礙. 煙臺大學學報(自然科學與工程版), 2022, 35(2): 170-175.
25.	Peng XM, Gao L, Huo SX, et al. The mechanism of memory enhancement of acteoside (verbascoside) in the senescent mouse model induced by a combination of D-gal and AlCl₃. Phytother Res, 2015, 29(8): 1137-1144.
26.	黃惠蓮, 陳莉. 肉蓯蓉總苷對血管性癡呆病人認知功能和血清p-tau蛋白表達的影響. 中西醫結合心腦血管病雜志, 2019, 17(8): 1248-1250.
27.	劉心朗, 周麗麗, 楊占君, 等. 肉蓯蓉總苷對SAMP8小鼠學習記憶能力及突觸可塑性的影響. 中華中醫藥學刊, 2022, 40(1): 110-115, 276, 277.
28.	賈建新, 宋嵬, 閆旭升, 等. 肉蓯蓉總苷對SAMP8小鼠空間學習記憶的影響及其機制. 包頭醫學院學報, 2014, 30(6): 6-8.

1. 張盼盼, 汪彥輝, 韓曉慶, 等. 丁苯酞對老年OSAHS患者氧化應激及認知功能的影響. 臨床耳鼻咽喉頭頸外科雜志, 2018, 32(18): 1422-1425.
2. 王樂, 張靜, 王彥, 等. 整夜睡眠血氧飽和度低于90%時間占總監測時間的百分比與阻塞性睡眠呼吸暫停低通氣常見合并癥的關系. 中華結核和呼吸雜志, 2020, 43(1): 63-67.
3. Vanek J, Prasko J, Genzor S, et al. Obstructive sleep apnea, depression and cognitive impairment. Sleep Med, 2020, 72: 50-58.
4. Xu LH, Yang YB, Chen J. The role of reactive oxygen species in cognitive impairment associated with sleep apnea. Exp Ther Med, 2020, 20(5): 4.
5. Zhou L, Chen P, Peng YT, et al. Role of oxidative stress in the neurocognitive dysfunction of obstructive sleep apnea syndrome. Oxid Med Cell Longev, 2016, 2016: 9626831.
6. 董文平, 任壽安. 血清核轉錄因子紅系2相關因子2、血紅素加氧酶-1水平與阻塞性睡眠呼吸暫停患者認知障礙的相關性研究. 中國呼吸與危重監護雜志, 2022, 21(7): 478-483.
7. 韓思璐, 馬迎民. 阻塞性睡眠呼吸暫停患者持續氣道正壓通氣治療相關吞氣癥和胃食管反流. 中國呼吸與危重監護雜志, 2018, 17(6): 639-643.
8. Jia JX, Yan XS, Cai ZP, et al. The effects of phenylethanoid glycosides, derived from Herba cistanche, on cognitive deficits and antioxidant activities in male SAMP8 mice. J Toxicol Environ Health A, 2017, 80(22): 1180-1186.
9. Yang JH, Ju BW, Hu JP. Effects of phenylethanoid glycosides extracted from Herba cistanches on the learning and memory of the APP/PSI transgenic mice with Alzheimer's disease. Biomed Res Int, 2021, 2021: 1291549.
10. 王璐, 白雨朦, 李曉宇, 等. 肉蓯蓉總苷對阿爾茨海默病模型大鼠學習認知功能和氧化應激的影響. 解剖學雜志, 2020, 43(3): 194-199.
11. 王紅陽, 韓曉慶, 趙雅寧, 等. 重度間歇低氧大鼠學習記憶功能與氧化應激的關系. 中華結核和呼吸雜志, 2012, 35(1): 24-28.
12. 張盼盼, 韓曉慶, 王紅陽, 等. 間歇低氧大鼠神經元特異性烯醇化酶蛋白的表達及其對學習記憶功能的影響. 中華行為醫學與腦科學雜志, 2014, 23(4): 303-306.
13. Patel A, Chong DJ. Obstructive sleep apnea: cognitive outcomes. Clin Geriatr Med, 2021, 37(3): 457-467.
14. Tóthová ?, Celec P, Mucska I, et al. Short-term effects of continuous positive airway pressure on oxidative stress in severe sleep apnea. Sleep Breath, 2019, 23(3): 857-863.
15. 馬曉蓉, 張靜宜, 宗運之, 等. 無創呼吸機治療對阻塞性睡眠呼吸暫停低通氣綜合征患者骨代謝和氧化應激影響的研究. 中國呼吸與危重監護雜志, 2022, 21(10): 731-736.
16. Rosenzweig I, Glasser M, Crum WR, et al. Changes in neurocognitive architecture in patients with obstructive sleep apnea treated with continuous positive airway pressure. EBioMedicine, 2016, 7: 221-229.
17. 韓曉慶, 張盼盼, 李琳, 等. 依達拉奉對重度間歇低氧大鼠認知功能及Nogo-A表達的影響. 中國中西醫結合急救雜志, 2017, 24(6): 570-574.
18. Abdel-Wahab BA, Abdel-Wahab MM. Protective effect of resveratrol against chronic intermittent hypoxia-induced spatial memory deficits, hippocampaloxidative DNA damage and increased p47Phox NADPH oxidase expression in young rats. Behav Brain Res, 2016, 305: 65-75.
19. Wang FJ, Li RY, Tu PF, et al. Total glycosides of Cistanche deserticola promote neurological function recovery by inducing neurovascular regeneration via Nrf-2/Keap-1 pathway in MCAO/R rats. Front Pharmacol, 2020, 11: 236.
20. 薛海燕, 焦嬋媛, 姚軍. 肉蓯蓉總苷藥理作用的研究現狀. 中國臨床藥理學雜志, 2018, 34(4): 486-488.
21. Ling JZ, Yu Q, Li YN, et al. Edaravone improves intermittent hypoxia-induced cognitive impairment and hippocampal damage in rats. Biol Pharm Bull, 2020, 43(8): 1196-1201.
22. Elliot-Portal E, Laouafa S, Arias-Reyes C, et al. Brain-derived erythropoietin protects from intermittent hypoxia-induced cardiorespiratory dysfunction and oxidative stress in mice. Sleep, 2018, 41(7): zsy072.
23. 樊燕燕, 張石在. 肉蓯蓉總苷通過調控lncRNA GAS5保護神經細胞缺血再灌注損傷. 中成藥, 2022, 44(7): 2320-2324.
24. 劉恩寵, 田原, 韋瑤, 等. 肉蓯蓉毛蕊花苷改善腦低灌注致小鼠認知功能障礙. 煙臺大學學報(自然科學與工程版), 2022, 35(2): 170-175.
25. Peng XM, Gao L, Huo SX, et al. The mechanism of memory enhancement of acteoside (verbascoside) in the senescent mouse model induced by a combination of D-gal and AlCl₃. Phytother Res, 2015, 29(8): 1137-1144.
26. 黃惠蓮, 陳莉. 肉蓯蓉總苷對血管性癡呆病人認知功能和血清p-tau蛋白表達的影響. 中西醫結合心腦血管病雜志, 2019, 17(8): 1248-1250.
27. 劉心朗, 周麗麗, 楊占君, 等. 肉蓯蓉總苷對SAMP8小鼠學習記憶能力及突觸可塑性的影響. 中華中醫藥學刊, 2022, 40(1): 110-115, 276, 277.
28. 賈建新, 宋嵬, 閆旭升, 等. 肉蓯蓉總苷對SAMP8小鼠空間學習記憶的影響及其機制. 包頭醫學院學報, 2014, 30(6): 6-8.

Chinese Journal of Respiratory and Critical Care Medicine

Effect of total glucosides of Cistanche deserticola on oxidative stress and cognitive function in rats with intermittent hypoxia

Abstract Full text Figures/Tables Video References Cited by

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