The characteristics of amino acid levels in peripheral blood of patients with COPD by tandem mass spectrometry_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

LIU Jia ¹ , LIU Haiwen ² , YAO Ting ³ ,  PAN Dianzhu ³

1. Graduate School of Jinzhou Medical University, Jinzhou, Liaoning 121000, P. R. China;
2. Central Laboratory, The Fist Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P. R. China;
3. Department of Respiratory Medicine, The Fist Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P. R. China;

Corresponding?author：

PAN Dianzhu, Email: pandianzhu@163.com

Keywords：

Chronic obstructive pulmonary disease; Metabolomics; Metabolism of amino acid; Tandem mass spectrometry

DOI：

10.7507/1671-6205.202011043

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective Tandem mass spectrometry is used to observe the changes in amino acids level in peripheral blood of patients with chronic obstructive pulmonary disease (COPD) of different severity, and explore the related factors that affect the level of amino acids in COPD patients.Methods A collection of 99 COPD patients from the First Affiliated Hospital of Jinzhou Medical University between May 2020 and August 2020 were divided into GOLD Ⅰ/Ⅱ group, GOLD Ⅲ group and GOLD Ⅳ group according to the results of their lung function. Thirty healthy physical examination subjects during the same period were enrolled as a healthy control group. Peripheral amino acids were detected by liquid chromatography-tandem mass spectrometry (LC-MS).Results The metabolism of 11 amino acids was correlated with the onset of COPD and the disorder of amino acid metabolism became more significant with the aggravation of the disease, and branched-chain amino acids (leucine, valine) had statistically significant differences in the COPD patients with different GOLD grades (P<0.05 and VIP>1). The difference between glutamate and glutamine was statistically significant only in GOLD Ⅳ stage (P<0.05 and VIP>1). The content of tyrosine and phenylalanine gradually increased with the increase of disease severity, and had significant difference in GOLD stage Ⅳ (P<0.05).Conclusions COPD patients with different GOLD grades have obvious amino acid metabolism disorders, including insufficient intake of essential amino acids and increased amino acids related to muscle protein catabolism. Understanding the mechanism between amino acid metabolism and COPD may provide a new direction for the diagnosis and treatment of the disease.

Citation： LIU Jia, LIU Haiwen, YAO Ting, PAN Dianzhu. The characteristics of amino acid levels in peripheral blood of patients with COPD by tandem mass spectrometry. Chinese Journal of Respiratory and Critical Care Medicine, 2021, 20(8): 542-548. doi: 10.7507/1671-6205.202011043 Copy

1.	Ran N, Pang Z, Gu Y, et al. An updated overview of metabolomic profile changes in chronic obstructive pulmonary disease. Metabolites, 2019, 9(6): 111.
2.	Quaderi SA, Hurst JR. The unmet global burden of COPD. Glob Health Epidemiol Genom, 2018, 3: e4.
3.	Cardoso J, Coelho R, Rocha C, et al. Prediction of severe exacerbations and mortality in COPD: the role of exacerbation history and inspiratory capacity/total lung capacity ratio. Int J Chron Obstruct Pulmon Dis, 2018, 13: 1105-1113.
4.	Psychogios N, Hau DD, Peng J, et al. The human serum metabolome. PLoS One, 2011, 6(2): e16957.
5.	Kilk K, Aug A, Ottas A, et al. Phenotyping of chronic obstructive pulmonary disease based on the integration of metabolomes and clinical characteristics. Int J Mol Sci, 2018, 19(3): 666.
6.	Chen Q, Deeb RS, Ma Y, et al. Serum metabolite biomarkers discriminate healthy smokers from COPD smokers. PLoS One, 2015, 10(12): e0143937.
7.	Wang L, Tang Y, Liu S, et al. Metabonomic profiling of serum and urine by ¹H NMR-based spectroscopy discriminates patients with chronic obstructive pulmonary disease and healthy individuals. PLoS One, 2013, 8(6): e65675.
8.	Ren X, Ma S, Wang J, et al. Comparative effects of dexamethasone and bergenin on chronic bronchitis and their anti-inflammatory mechanisms based on NMR metabolomics. Mol Biosyst, 2016, 12(6): 1938-1947.
9.	Deja S, Porebska I, Kowal A, et al. Metabolomics provide new insights on lung cancer staging and discrimination from chronic obstructive pulmonary disease. J Pharm Biomed Anal, 2014, 100: 369-380.
10.	Nobakht MGB, Aliannejad R, Rezaei-Tavirani M, et al. The metabolomics of airway diseases, including COPD, asthma and cystic fibrosis. Biomarkers, 2015, 20(1): 5-16.
11.	Ubhi BK, Riley JH, Shaw PA, et al. Metabolic profiling detects biomarkers of protein degradation in COPD patients. Eur Respir J, 2012, 40(2): 345-355.
12.	Van AM, Heijink M, Mayboroda OA, et al. Dynamic differences in dietary polyunsaturated fatty acid metabolism in sputum of COPD patients and controls. Biochim Biophys Acta Mol Cell Biol Lipids, 2019, 1864(3): 224-233.
13.	Ubhi BK, Cheng KK, Dong J, et al. Targeted metabolomics identifies perturbations in amino acid metabolism that sub-classify patients with COPD. Mol Biosyst, 2012, 8(12): 3125-3133.
14.	中華醫學會呼吸病學分會慢性阻塞性肺疾病學組, 中國醫師協會呼吸醫師分會慢性阻塞性肺疾病工作委員會. 慢性阻塞性肺疾病診治指南(2021 年修訂版). 中華結核和呼吸雜志, 2021, 44(3): 170-205.
15.	荊雨. 基于干血滴質譜代謝組學方法區分結直腸癌和結直腸息肉的研究[D]. 錦州醫科大學, 2017.
16.	Vahid I, Abdolali B, Fatemeh M, et al. The effects of branch-chain amino acids on fatigue in the athletes. Interv Med Appl Sci, 2018, 10(4): 233-235.
17.	Hole?ek M. Branched-chain amino acids in health and disease: metabolism, alterations in blood plasma, and as supplements. Nutr Metab (Lond), 2018, 15: 33.
18.	Yoneda T, Yoshikawa M, Fu A, et al. Plasma levels of amino acids and hypermetabolism in patients with chronic obstructive pulmonary disease. Nutrition, 2001, 17(2): 95-99.
19.	Engelen MP, De Castro CL, Rutten EP, et al. Enhanced anabolic response to milk protein sip feeding in elderly subjects with COPD is associated with a reduced splanchnic extraction of multiple amino acids. Clin Nutr, 2012, 31(5): 616-624.
20.	Oliveira GP, De Abreu MG, Pelosi P, et al. Exogenous glutamine in respiratory diseases: myth or reality?. Nutrients, 2016, 8(2): 76.
21.	張凡, 嚴會超, 王修啟, 等. 堿性氨基酸轉運載體基因在骨骼肌中的表達規律及其調控. 動物營養學報, 2019, 31(2): 567-574.
22.	賀洪, 劉建紅, 黃金麗, 等. 運動中補充支鏈氨基酸對肌肉代謝的影響. 中國運動醫學雜志, 2004, 23(4): 461-464.
23.	Vendelbo MH, M?ller AB, Christensen B, et al. Fasting increases human skeletal muscle net phenylalanine release and this is associated with decreased mTOR signaling. PLoS One, 2014, 9(7): 102031.
24.	Kuo WK, Liu YC, Chu CM, et al. Amino acid-based metabolic indexes identify patients with chronic obstructive pulmonary disease and further discriminates patients in advanced BODE stages. Int J Chron Obstruct Pulmon Dis, 2019, 14: 2257-2266.
25.	Sirni? P, V?yrynen JP, Klintrup K, et al. Alterations in serum amino-acid profile in the progression of colorectal cancer: associations with systemic inflammation, tumour stage and patient survival. Br J Cancer, 2019, 120(2): 238-246.

1. Ran N, Pang Z, Gu Y, et al. An updated overview of metabolomic profile changes in chronic obstructive pulmonary disease. Metabolites, 2019, 9(6): 111.
2. Quaderi SA, Hurst JR. The unmet global burden of COPD. Glob Health Epidemiol Genom, 2018, 3: e4.
3. Cardoso J, Coelho R, Rocha C, et al. Prediction of severe exacerbations and mortality in COPD: the role of exacerbation history and inspiratory capacity/total lung capacity ratio. Int J Chron Obstruct Pulmon Dis, 2018, 13: 1105-1113.
4. Psychogios N, Hau DD, Peng J, et al. The human serum metabolome. PLoS One, 2011, 6(2): e16957.
5. Kilk K, Aug A, Ottas A, et al. Phenotyping of chronic obstructive pulmonary disease based on the integration of metabolomes and clinical characteristics. Int J Mol Sci, 2018, 19(3): 666.
6. Chen Q, Deeb RS, Ma Y, et al. Serum metabolite biomarkers discriminate healthy smokers from COPD smokers. PLoS One, 2015, 10(12): e0143937.
7. Wang L, Tang Y, Liu S, et al. Metabonomic profiling of serum and urine by ¹H NMR-based spectroscopy discriminates patients with chronic obstructive pulmonary disease and healthy individuals. PLoS One, 2013, 8(6): e65675.
8. Ren X, Ma S, Wang J, et al. Comparative effects of dexamethasone and bergenin on chronic bronchitis and their anti-inflammatory mechanisms based on NMR metabolomics. Mol Biosyst, 2016, 12(6): 1938-1947.
9. Deja S, Porebska I, Kowal A, et al. Metabolomics provide new insights on lung cancer staging and discrimination from chronic obstructive pulmonary disease. J Pharm Biomed Anal, 2014, 100: 369-380.
10. Nobakht MGB, Aliannejad R, Rezaei-Tavirani M, et al. The metabolomics of airway diseases, including COPD, asthma and cystic fibrosis. Biomarkers, 2015, 20(1): 5-16.
11. Ubhi BK, Riley JH, Shaw PA, et al. Metabolic profiling detects biomarkers of protein degradation in COPD patients. Eur Respir J, 2012, 40(2): 345-355.
12. Van AM, Heijink M, Mayboroda OA, et al. Dynamic differences in dietary polyunsaturated fatty acid metabolism in sputum of COPD patients and controls. Biochim Biophys Acta Mol Cell Biol Lipids, 2019, 1864(3): 224-233.
13. Ubhi BK, Cheng KK, Dong J, et al. Targeted metabolomics identifies perturbations in amino acid metabolism that sub-classify patients with COPD. Mol Biosyst, 2012, 8(12): 3125-3133.
14. 中華醫學會呼吸病學分會慢性阻塞性肺疾病學組, 中國醫師協會呼吸醫師分會慢性阻塞性肺疾病工作委員會. 慢性阻塞性肺疾病診治指南(2021 年修訂版). 中華結核和呼吸雜志, 2021, 44(3): 170-205.
15. 荊雨. 基于干血滴質譜代謝組學方法區分結直腸癌和結直腸息肉的研究[D]. 錦州醫科大學, 2017.
16. Vahid I, Abdolali B, Fatemeh M, et al. The effects of branch-chain amino acids on fatigue in the athletes. Interv Med Appl Sci, 2018, 10(4): 233-235.
17. Hole?ek M. Branched-chain amino acids in health and disease: metabolism, alterations in blood plasma, and as supplements. Nutr Metab (Lond), 2018, 15: 33.
18. Yoneda T, Yoshikawa M, Fu A, et al. Plasma levels of amino acids and hypermetabolism in patients with chronic obstructive pulmonary disease. Nutrition, 2001, 17(2): 95-99.
19. Engelen MP, De Castro CL, Rutten EP, et al. Enhanced anabolic response to milk protein sip feeding in elderly subjects with COPD is associated with a reduced splanchnic extraction of multiple amino acids. Clin Nutr, 2012, 31(5): 616-624.
20. Oliveira GP, De Abreu MG, Pelosi P, et al. Exogenous glutamine in respiratory diseases: myth or reality?. Nutrients, 2016, 8(2): 76.
21. 張凡, 嚴會超, 王修啟, 等. 堿性氨基酸轉運載體基因在骨骼肌中的表達規律及其調控. 動物營養學報, 2019, 31(2): 567-574.
22. 賀洪, 劉建紅, 黃金麗, 等. 運動中補充支鏈氨基酸對肌肉代謝的影響. 中國運動醫學雜志, 2004, 23(4): 461-464.
23. Vendelbo MH, M?ller AB, Christensen B, et al. Fasting increases human skeletal muscle net phenylalanine release and this is associated with decreased mTOR signaling. PLoS One, 2014, 9(7): 102031.
24. Kuo WK, Liu YC, Chu CM, et al. Amino acid-based metabolic indexes identify patients with chronic obstructive pulmonary disease and further discriminates patients in advanced BODE stages. Int J Chron Obstruct Pulmon Dis, 2019, 14: 2257-2266.
25. Sirni? P, V?yrynen JP, Klintrup K, et al. Alterations in serum amino-acid profile in the progression of colorectal cancer: associations with systemic inflammation, tumour stage and patient survival. Br J Cancer, 2019, 120(2): 238-246.

Chinese Journal of Respiratory and Critical Care Medicine

The characteristics of amino acid levels in peripheral blood of patients with COPD by tandem mass spectrometry

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content