The effect of dust fine particles on TNF-α, MMP, TGF-β<sub>1</sub>, and collagens in the lung tissue of rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

LI Yi ¹ ,  LEI Fengfeng ² , HOU Yinhuan ³ , SANG Yingzhu ²

1. Department of Respiration In Cadre Ward, The People’s Hospital of Gansu Province, Lanzhou, Gansu 730000, P. R. China;
2. Department of Respiratory Medicine, The People’s Hospital of Gansu Province, Lanzhou, Gansu 730000, P. R. China;
3. Department of Respiratory Medicine, Jingning County Hospital, Pingliang, Gansu 744000, P. R. China;

Corresponding?author：

LEI Fengfeng, Email: syingzhu@126.com

Keywords：

Sandstorm; Tumor necrosis factor-α; Matrix metalloproteinase; Transforming growth factor-β₁; Pulmonary collagen

DOI：

10.7507/1671-6205.201910012

Video：

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Objective To investigate the effect of dust fine particles on tumor necrosis factor-α (TNF-α), matrix metalloproteinase (MMP), transforming growth factor-β₁ (TGF-β₁), and collagens in the lung tissue of rats.Methods According to random number table method, 96 male Wistar rats were divided into an untreated control group, a treated control group and an experimental group, with 32 rats in each group. The experimental group was exposed to the wind tunnel simulation of sandstorm (5 days per week, 5 hours per day); the untreated control group was put in the standard living environment next to the wind tunnel; the treated control group was exposed to the same wind tunnel simulation of sandstorm for 5 hours every day, the speed of wind was the same as the experimental group, but without dust; On the 30^th, 60^th, 90^th, and 120^th day, the levels of TNF-α, MMP-2, MMP-9, TGF-β₁, lung collagen type Ⅰ and Ⅲ in the lung tissue of rats were determined by enzyme linked immunosorbent assay.Results Compared with the untreated control group and the treated control group, the content of TNF-α was higher in the experimental group on 30th, 60^th, 90^th and 120^th day (all P<0.05). The contents of MMP-9 and MMP-2 in the experimental group on 60^th and 90^th day were significantly higher than those in the untreated group and the treated control group, respectively (all P<0.05). On the 30^th, 60^th, 90^th, and 120^th day, the content of TGF-β₁ in the experimental group was significantly higher compared with the two control groups (all P<0.05). The contents of lung collagen type Ⅰ and type Ⅲ were higher in the experimental group on 60^th, 90^th and 120^th day, respectively, compared with the two control groups (all P<0.05).Conclusions The strong sandstorm environmental exposure to a certain period of time can promote lung interstitial collagen deposition in rat. With the prolonged exposure time, the deposition of collagen increases. TNF-α, MMP-2, MMP-9 and TGF-β₁ may all participate and induce the process of pulmonary fibrosis.

Citation： LI Yi, LEI Fengfeng, HOU Yinhuan, SANG Yingzhu. The effect of dust fine particles on TNF-α, MMP, TGF-β₁, and collagens in the lung tissue of rats. Chinese Journal of Respiratory and Critical Care Medicine, 2020, 19(3): 264-269. doi: 10.7507/1671-6205.201910012 Copy

1.	劉新春, 鐘玉婷, 何清, 等. 塔克拉瑪干沙漠腹地沙塵暴過程大氣顆粒物濃度及影響因素分析. 中國沙漠, 2011, 31(6): 1548-1553.
2.	Atkinson RW, Kang S, Anderson HR, et al. Epidemiological time series studies of PM2.5 and daily mortality and hospital admissions: a systematic review and meta-analysis. Thorax, 2014, 69(7): 660-665.
3.	Cao XJ, Lei FF, Liu H, et al. Effects of dust storm fine particle-inhalation on the respiratory, cardiovascular, endocrine, hematological, and digestive systems of rats. Chin Med J (Engl), 2018, 131(20): 2482-2485.
4.	雷豐豐, 黨雅梅, 張長青. 沙塵暴對大鼠肺組織影響的病理學觀察. 中華病理學雜志, 2015, 44(3): 199-201.
5.	雷豐豐, 王學斌, 劉華. 沙塵氣溶膠暴露對大鼠肺功能及肺組織病理學影響的研究. 中華醫學雜志, 2015, 95(32): 2634-2638.
6.	賈曉鵬, 王海兵. 甘肅河西地區大氣沙塵成分及影響分析. 中國沙漠, 2009, 29(1): 156-161.
7.	邵龍義, 李衛軍, 楊書申, 等. 2002 年春季北京特大沙塵暴顆粒的礦物組成分析. 中國科學 D 輯: 地球科學, 2007, 37(2): 215-221.
8.	Lei FF, Luo WY, Dong ZB, et al. An investigation of the effects of dust storms on rat lung using HRCT and blood gas analysis. Sci Cold Arid Regions, 2016, 8(4): 319-324.
9.	Policard A, Collet A. Deposition of silicosis dust in the lungs of inhabitant of the Saharan regions. AMA Arch Ind Hyg Occup Med, 1952, 5(6): 527-534.
10.	李盛, 王金玉, 李普, 等. 沙塵天氣的呼吸系統健康效應及機制研究進展. 環境與健康雜志, 2019, 36(1): 78-82.
11.	Craig VJ, Zhang L, Hagood JS, et al. Matrix metalloproteinases as therapeutic targets for idiopathic pulmonary fibrosis. Am J Respir Cell Mol Biol, 2015, 53(5): 585-600.
12.	Kang H. Role of microRNAs in TGF-β singling pathway-mediated pulmonary fibrosis. Int J Mol Sci, 2017, 18(12): E2527.
13.	Kasabova M, Joulin-Giet A, Lecaille F, et al. Regulation of TGF-β1-driven differentiation of human lung fibroblasts: emerging roles of cathepsin B and cystatin C. J Biol Chem, 2014, 289(23): 16239-16251.
14.	Ohno Y, Nakanishi T, Umigai N, et al. Oral administration of crocetin prevents inner retinal damage induced by N-methyl-D-aspartate in mice. Eur J Pharmacol, 2012, 690(1-3): 84-89.
15.	張偉, 鄭建, 朱雪, 等. 益氣類中藥對肺纖維化大鼠模型肺組織中 TNF-α、IL-8 的影響. 中華中醫藥學刊, 2014, 32(10): 2311-2313.
16.	Yao HW, Xie QM, Chen JQ, et al. TGF-beta1 induces alveolar epithelial to mesenchymal transition in vitro. Life Sci, 2004, 76(1): 29-37.
17.	章巍, 劉新民, 李海潮, 等. 博來霉素致大鼠肺纖維化模型肺組織 MMP-2、MMP-9、MT1-MMP 及 TIMP-1、TIMP-2 的表達. 北京大學學報(醫學版), 2002, 34(6): 716-721.
18.	Wang A, Wang F, Yin Y, et al. Dexamethasone reduces serum level of IL-17 in bleomycin-A5-induced rats model of pulmonary fibrosis. Artif Cells Nanomed Biotechnol, 2018, 46(4): 783-787.
19.	Yang D, Yuan W, Lv C, et al. Dihydroartemisinin suppresses inflammation and fibrosis in bleomycin-induced pulmonary fibrosis in rats. Int J Clin Exp Pathol, 2015, 8(2): 1270-1281.
20.	安方玉, 顏春魯, 劉永琦, 等. 瀉肺湯對肺纖維化大鼠 TGF-β1/Smads 信號轉導通路的干預研究. 中華中醫藥雜志(原中國醫藥學報), 2018, 33(6): 2641-2645.
21.	胡洋, 李惠萍. PM2.5 對肺纖維化的影響. 臨床內科雜志, 2015, 32(4): 228-230.
22.	Fujita H, Sakamoto N, Ishimatsu Y, et al. Effects of doxycycline on production of growth factors and matrix metalloproteinases in pulmonary fibrosis. Respiration, 2011, 81(5): 420-430.
23.	陳雅萍, 宋志芳. 塵源性炎癥反應與矽肺纖維化的關系. 中國工業醫學雜志, 1999, 12(2): 75-77.
24.	Zheng X, Qi C, Zhang S, et al. TGF-β1 induces Fstl1 via the Smad3-c-Jun pathway in lung fibroblasts. Am J Physiol Lung Cell Mol Physiol, 2017, 313(2): L240-L251.

1. 劉新春, 鐘玉婷, 何清, 等. 塔克拉瑪干沙漠腹地沙塵暴過程大氣顆粒物濃度及影響因素分析. 中國沙漠, 2011, 31(6): 1548-1553.
2. Atkinson RW, Kang S, Anderson HR, et al. Epidemiological time series studies of PM2.5 and daily mortality and hospital admissions: a systematic review and meta-analysis. Thorax, 2014, 69(7): 660-665.
3. Cao XJ, Lei FF, Liu H, et al. Effects of dust storm fine particle-inhalation on the respiratory, cardiovascular, endocrine, hematological, and digestive systems of rats. Chin Med J (Engl), 2018, 131(20): 2482-2485.
4. 雷豐豐, 黨雅梅, 張長青. 沙塵暴對大鼠肺組織影響的病理學觀察. 中華病理學雜志, 2015, 44(3): 199-201.
5. 雷豐豐, 王學斌, 劉華. 沙塵氣溶膠暴露對大鼠肺功能及肺組織病理學影響的研究. 中華醫學雜志, 2015, 95(32): 2634-2638.
6. 賈曉鵬, 王海兵. 甘肅河西地區大氣沙塵成分及影響分析. 中國沙漠, 2009, 29(1): 156-161.
7. 邵龍義, 李衛軍, 楊書申, 等. 2002 年春季北京特大沙塵暴顆粒的礦物組成分析. 中國科學 D 輯: 地球科學, 2007, 37(2): 215-221.
8. Lei FF, Luo WY, Dong ZB, et al. An investigation of the effects of dust storms on rat lung using HRCT and blood gas analysis. Sci Cold Arid Regions, 2016, 8(4): 319-324.
9. Policard A, Collet A. Deposition of silicosis dust in the lungs of inhabitant of the Saharan regions. AMA Arch Ind Hyg Occup Med, 1952, 5(6): 527-534.
10. 李盛, 王金玉, 李普, 等. 沙塵天氣的呼吸系統健康效應及機制研究進展. 環境與健康雜志, 2019, 36(1): 78-82.
11. Craig VJ, Zhang L, Hagood JS, et al. Matrix metalloproteinases as therapeutic targets for idiopathic pulmonary fibrosis. Am J Respir Cell Mol Biol, 2015, 53(5): 585-600.
12. Kang H. Role of microRNAs in TGF-β singling pathway-mediated pulmonary fibrosis. Int J Mol Sci, 2017, 18(12): E2527.
13. Kasabova M, Joulin-Giet A, Lecaille F, et al. Regulation of TGF-β1-driven differentiation of human lung fibroblasts: emerging roles of cathepsin B and cystatin C. J Biol Chem, 2014, 289(23): 16239-16251.
14. Ohno Y, Nakanishi T, Umigai N, et al. Oral administration of crocetin prevents inner retinal damage induced by N-methyl-D-aspartate in mice. Eur J Pharmacol, 2012, 690(1-3): 84-89.
15. 張偉, 鄭建, 朱雪, 等. 益氣類中藥對肺纖維化大鼠模型肺組織中 TNF-α、IL-8 的影響. 中華中醫藥學刊, 2014, 32(10): 2311-2313.
16. Yao HW, Xie QM, Chen JQ, et al. TGF-beta1 induces alveolar epithelial to mesenchymal transition in vitro. Life Sci, 2004, 76(1): 29-37.
17. 章巍, 劉新民, 李海潮, 等. 博來霉素致大鼠肺纖維化模型肺組織 MMP-2、MMP-9、MT1-MMP 及 TIMP-1、TIMP-2 的表達. 北京大學學報(醫學版), 2002, 34(6): 716-721.
18. Wang A, Wang F, Yin Y, et al. Dexamethasone reduces serum level of IL-17 in bleomycin-A5-induced rats model of pulmonary fibrosis. Artif Cells Nanomed Biotechnol, 2018, 46(4): 783-787.
19. Yang D, Yuan W, Lv C, et al. Dihydroartemisinin suppresses inflammation and fibrosis in bleomycin-induced pulmonary fibrosis in rats. Int J Clin Exp Pathol, 2015, 8(2): 1270-1281.
20. 安方玉, 顏春魯, 劉永琦, 等. 瀉肺湯對肺纖維化大鼠 TGF-β1/Smads 信號轉導通路的干預研究. 中華中醫藥雜志(原中國醫藥學報), 2018, 33(6): 2641-2645.
21. 胡洋, 李惠萍. PM2.5 對肺纖維化的影響. 臨床內科雜志, 2015, 32(4): 228-230.
22. Fujita H, Sakamoto N, Ishimatsu Y, et al. Effects of doxycycline on production of growth factors and matrix metalloproteinases in pulmonary fibrosis. Respiration, 2011, 81(5): 420-430.
23. 陳雅萍, 宋志芳. 塵源性炎癥反應與矽肺纖維化的關系. 中國工業醫學雜志, 1999, 12(2): 75-77.
24. Zheng X, Qi C, Zhang S, et al. TGF-β1 induces Fstl1 via the Smad3-c-Jun pathway in lung fibroblasts. Am J Physiol Lung Cell Mol Physiol, 2017, 313(2): L240-L251.

Chinese Journal of Respiratory and Critical Care Medicine

The effect of dust fine particles on TNF-α, MMP, TGF-β₁, and collagens in the lung tissue of rats

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Chinese Journal of Respiratory and Critical Care Medicine

The effect of dust fine particles on TNF-α, MMP, TGF-β1, and collagens in the lung tissue of rats

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The effect of dust fine particles on TNF-α, MMP, TGF-β₁, and collagens in the lung tissue of rats