16S rRNA-base analysis of bacterial diversity in the induced sputum of patients with acute exacerbation of chronic obstructive pulmonary disease_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

QI Yujing , WANG Zhe , SUN Xuejiao ^# , BIN Yanfei ^# , LI Yinghua ^# ,  HE Zhiyi

Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of GuangXi Medical University, Nanning, Guangxi 530021, P. R. China;

Corresponding?author：

HE Zhiyi, Email: zhiyi-river@163.com

Keywords：

Chronic obstructive pulmonary disease; Acute exacerbations; Induced sputum; Microbiome

DOI：

10.7507/1671-6205.201909075

Video：

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Objective To explore the characteristics of induced sputum microbiome in the patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD).Methods Induced sputum samples from 55 patients with AECOPD and 45 patients with stable COPD were analyzed by sequencing of 16S rRNA gene. Microbiota was measured by alpha diversity, beta diversity and LDA effect size analysis (LefSe).Results The microbiome diversity of induced sputum in the AECOPD group was lower than that in the stable COPD group. The microbiome richness in the AECOPD group was higher than that in the stable COPD group. The microbiome structure changed in the AECOPD group compared with the stable COPD group. The proportion of some common pathogens got enriched. The levels of hypersensitive C reactive protein (hs-CRP), interleukin-8 (IL-8), tumor necrosis factor alpha (TNF-α) and Global Initative for Chronic Obstructive Lung Disease (GOLD) grade were negatively related to the diversity of microbiome in the AECOPD group.Conclusions The microbiome diversity of induced sputum in AECOPD patients is decreased, and is negatively correlated with the levels of hs-CRP, IL-8, TNF-α and GOLD grade. There are differences in the microbiome structure between AECOPD and stable COPD patients. Some enrichment of common pathogens are found in the induced sputum of patients with AECOPD. These results suggest that there is a significant bacterial dysbiosis in patients with AECOPD.

Citation： QI Yujing, WANG Zhe, SUN Xuejiao, BIN Yanfei, LI Yinghua, HE Zhiyi. 16S rRNA-base analysis of bacterial diversity in the induced sputum of patients with acute exacerbation of chronic obstructive pulmonary disease. Chinese Journal of Respiratory and Critical Care Medicine, 2020, 19(4): 359-365. doi: 10.7507/1671-6205.201909075 Copy

1.	van Geffen WH, Kerstjens HA. Static and dynamic hyperinflation during severe acute exacerbations of chronic obstructive pulmonary disease. Int J Chron Obstruct Pulmon Dis, 2018, 13: 1269-1277.
2.	Vogelmeier CF, Criner GJ, Martinez FJ, et al. Global Strategy for the Diagnosis, Management, and Prevention of Chronic Obstructive Lung Disease 2017 Report. GOLD Executive Summary. Am J Respir Crit Care Med, 2017, 195(5): 557-582.
3.	Wedzicha JA, Brill SE, Allinson JP, et al. Mechanisms and impact of the frequent exacerbator phenotype in chronic obstructive pulmonary disease. BMC Med, 2013, 11: 181-190.
4.	Sethi S, Murphy TF. Infection in the pathogenesis and course of chronic obstructive pulmonary disease. N Engl J Med, 2008, 359(22): 2355-2365.
5.	Han MK, Huang YJ, Lipuma JJ, et al. Significance of the microbiome in obstructive lung disease. Thorax, 2012, 67(5): 456-463.
6.	Sethi S, Evans N, Grant BJ, et al. New strains of bacteria and exacerbations of chronic obstructive pulmonary disease. N Engl J Med, 2002, 347(7): 465-471.
7.	慢性阻塞性肺疾病急性加重(AECOPD)診治中國專家共識(2014年修訂版). 國際呼吸雜志, 2014, 34(1): 1-11.
8.	Pin I, Gibson PG, Kolendowicz R, et al. Use of induced sputum cell counts to investigate airway inflammation in asthma. Thorax, 1992, 47(1): 25-29.
9.	Quast C, Pruesse E, Yilmaz P, et al. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res, 2013, 41(Database issue): D590-D596.
10.	Lederberg J. Biological warfare. Emerg Infect Dis, 2001, 7(6): 1071-1072.
11.	Gill SR, Pop M, Deboy RT, et al. Metagenomic analysis of the human distal gut microbiome. Science, 2006, 312(5778): 1355-1359.
12.	Hilty M, Burke C, Pedro H, et al. Disordered microbial communities in asthmatic airways. PloS One, 2010, 5(1): e8578.
13.	Hansel TT, Johnston SL, Openshaw PJ. Microbes and mucosal immune responses in asthma. Lancet, 2013, 381(9869): 861-873.
14.	Marsland BJ, Gollwitzer ES. Host-microorganism interactions in lung diseases. Nat Rev Immunol, 2014, 14(12): 827-835.
15.	Millares L, Ferrari R, Gallego M, et al. Bronchial microbiome of severe COPD patients colonised by Pseudomonas aeruginosa. Eur J Clin Microbiol Infect Dis, 2014, 33(7): 1101-1111.
16.	Rangelov K, Sethi S. Role of infections. Clin Chest Med, 2014, 35(1): 87-100.
17.	李乃健, 李冰, 冉丕鑫. 大氣顆粒物對肺部微生態的影響及在慢性阻塞性肺疾病發病中的作用. 中國呼吸與危重監護雜志, 2018, 17(2): 206-209.
18.	Charlson ES, Bittinger K, Haas AR, et al. Topographical continuity of bacterial populations in the healthy human respiratory tract. Am J Respir Crit Care Med, 2011, 184(8): 957-963.
19.	Wang Z, Bafadhel M, Haldar K, et al. Lung microbiome dynamics in COPD exacerbations. Eur Respir J, 2016, 47(4): 1082-1092.
20.	Sze MA, Dimitriu PA, Suzuki M, et al. Host response to the lung microbiome in chronic obstructive pulmonary disease. Am J Respir Crit Care Med, 2015, 192(4): 438-445.
21.	Galiana A, Aguirre E, Rodriguez JC, et al. Sputum microbiota in moderate versus severe patients with COPD. Eur Respir J, 2014, 43(6): 1787-1790.
22.	Pragman AA, Lyu T, Baller JA, et al. The lung tissue microbiota of mild and moderate chronic obstructive pulmonary disease. Microbiome, 2018, 6(1): 7.
23.	Huang YJ, Nelson CE, Brodie EL, et al. Airway microbiota and bronchial hyperresponsiveness in patients with suboptimally controlled asthma. J Allergy Clin Immunol, 2011, 127(2): 372-373.
24.	Huang YJ, Sethi S, Murphy T, et al. Airway microbiome dynamics in exacerbations of chronic obstructive pulmonary disease. J Clin Microbiol, 2014, 52(8): 2813-2823.

1. van Geffen WH, Kerstjens HA. Static and dynamic hyperinflation during severe acute exacerbations of chronic obstructive pulmonary disease. Int J Chron Obstruct Pulmon Dis, 2018, 13: 1269-1277.
2. Vogelmeier CF, Criner GJ, Martinez FJ, et al. Global Strategy for the Diagnosis, Management, and Prevention of Chronic Obstructive Lung Disease 2017 Report. GOLD Executive Summary. Am J Respir Crit Care Med, 2017, 195(5): 557-582.
3. Wedzicha JA, Brill SE, Allinson JP, et al. Mechanisms and impact of the frequent exacerbator phenotype in chronic obstructive pulmonary disease. BMC Med, 2013, 11: 181-190.
4. Sethi S, Murphy TF. Infection in the pathogenesis and course of chronic obstructive pulmonary disease. N Engl J Med, 2008, 359(22): 2355-2365.
5. Han MK, Huang YJ, Lipuma JJ, et al. Significance of the microbiome in obstructive lung disease. Thorax, 2012, 67(5): 456-463.
6. Sethi S, Evans N, Grant BJ, et al. New strains of bacteria and exacerbations of chronic obstructive pulmonary disease. N Engl J Med, 2002, 347(7): 465-471.
7. 慢性阻塞性肺疾病急性加重(AECOPD)診治中國專家共識(2014年修訂版). 國際呼吸雜志, 2014, 34(1): 1-11.
8. Pin I, Gibson PG, Kolendowicz R, et al. Use of induced sputum cell counts to investigate airway inflammation in asthma. Thorax, 1992, 47(1): 25-29.
9. Quast C, Pruesse E, Yilmaz P, et al. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res, 2013, 41(Database issue): D590-D596.
10. Lederberg J. Biological warfare. Emerg Infect Dis, 2001, 7(6): 1071-1072.
11. Gill SR, Pop M, Deboy RT, et al. Metagenomic analysis of the human distal gut microbiome. Science, 2006, 312(5778): 1355-1359.
12. Hilty M, Burke C, Pedro H, et al. Disordered microbial communities in asthmatic airways. PloS One, 2010, 5(1): e8578.
13. Hansel TT, Johnston SL, Openshaw PJ. Microbes and mucosal immune responses in asthma. Lancet, 2013, 381(9869): 861-873.
14. Marsland BJ, Gollwitzer ES. Host-microorganism interactions in lung diseases. Nat Rev Immunol, 2014, 14(12): 827-835.
15. Millares L, Ferrari R, Gallego M, et al. Bronchial microbiome of severe COPD patients colonised by Pseudomonas aeruginosa. Eur J Clin Microbiol Infect Dis, 2014, 33(7): 1101-1111.
16. Rangelov K, Sethi S. Role of infections. Clin Chest Med, 2014, 35(1): 87-100.
17. 李乃健, 李冰, 冉丕鑫. 大氣顆粒物對肺部微生態的影響及在慢性阻塞性肺疾病發病中的作用. 中國呼吸與危重監護雜志, 2018, 17(2): 206-209.
18. Charlson ES, Bittinger K, Haas AR, et al. Topographical continuity of bacterial populations in the healthy human respiratory tract. Am J Respir Crit Care Med, 2011, 184(8): 957-963.
19. Wang Z, Bafadhel M, Haldar K, et al. Lung microbiome dynamics in COPD exacerbations. Eur Respir J, 2016, 47(4): 1082-1092.
20. Sze MA, Dimitriu PA, Suzuki M, et al. Host response to the lung microbiome in chronic obstructive pulmonary disease. Am J Respir Crit Care Med, 2015, 192(4): 438-445.
21. Galiana A, Aguirre E, Rodriguez JC, et al. Sputum microbiota in moderate versus severe patients with COPD. Eur Respir J, 2014, 43(6): 1787-1790.
22. Pragman AA, Lyu T, Baller JA, et al. The lung tissue microbiota of mild and moderate chronic obstructive pulmonary disease. Microbiome, 2018, 6(1): 7.
23. Huang YJ, Nelson CE, Brodie EL, et al. Airway microbiota and bronchial hyperresponsiveness in patients with suboptimally controlled asthma. J Allergy Clin Immunol, 2011, 127(2): 372-373.
24. Huang YJ, Sethi S, Murphy T, et al. Airway microbiome dynamics in exacerbations of chronic obstructive pulmonary disease. J Clin Microbiol, 2014, 52(8): 2813-2823.

Chinese Journal of Respiratory and Critical Care Medicine

16S rRNA-base analysis of bacterial diversity in the induced sputum of patients with acute exacerbation of chronic obstructive pulmonary disease

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