Effects of Reduced Glutathione in Rats with Pulmonary Fibrosis_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

MaZhimin ¹ , LiXiaoling ² , WeiHuiqiang ¹ , HaoXiuling ¹ ,  GuoLiping ¹

1. Department of Respiratory Medicine, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 054000, China;
2. ;

Corresponding?author：

GuoLiping, Email: wzb630601@126.com

Keywords：

Pulmonary fibrosis; Reduced glutathione; Rats

DOI：

10.7507/1671-6205.2016094

Video：

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Objective To explore the antioxidant effects of reduced glutathione on rat pulmonary fibrosis compared with traditional corticosteroid. Methods One-hundred and eight healthy SD rats were randomly divided into 6 groups,ie. a control group,a model group,a dexamethasone group,a low-dose glutathione group,a middle-dose glutathione group,and a high-dose glutathione group,with 18 rats in each group. The pulmonary fibrosis model was established by intratrachially instillation of bleomycin in all rats except the control group. The severity of lung fibrosis was evaluated by HE staining and Masson staining of collagen,and measurement of glutathione,hydroxyproline,superoxide dismutase (SOD),glutathion peroxidase (GSH-Px)in lung tissue homogenate by photocolorimetric method. Results On 7^th day and 14^th day after bleomycin instillation, the severity of alveolitis in the model group,the dexamethasone group,and three glutathione intervention groups was significantly reduced compared with the control group (P<0.05). On 28 day, the severity of lung fibrosis was significantly reduced in the dexamethasone group and three glutathione intervention groups compared with the model group (P<0.05). On 7^th day,lung glutathione content was significantly lower in the model group compared with the control group (P<0.05), significantly higher in the dexamethasone group and three glutathione intervention groups compared with the model group (P<0.05), significantly lower in the dexamethasone group and the low-dose glutathione group compared with the control group (P<0.05), and significantly higher in the high-dose glutathione group compared with the dexamethasone group (P<0.05). On 7^th,14^th,and 28^th day,the hydroxyproline content in the dexamethasone group and three glutathione intervention groups decreased significantly compared with the model group (P<0.05). On 14^th day,the hydroxyproline content in the middle-dose and high-dose glutathione groups was significantly lower than that in the dexamethasone group (P<0.05). SOD and GSH-Px were significantly reduced in the model group compared with the control group on all time points (P<0.05),but significantly increased after intervention by different doses of glutathione (P<0.05). Conclusion Reduced glutathione can significantly reduce the degree of pulmonary fibrosis in rats,but has no obvious advantage over dexamethasone.

Citation： MaZhimin, LiXiaoling, WeiHuiqiang, HaoXiuling, GuoLiping. Effects of Reduced Glutathione in Rats with Pulmonary Fibrosis. Chinese Journal of Respiratory and Critical Care Medicine, 2016, 15(4): 401-405. doi: 10.7507/1671-6205.2016094 Copy

1.	支開葉,康永,倪艷,等.肺纖方干預博萊霉素致肺纖維化大鼠的實驗研究.中國實驗方劑學雜志,2012,18:212-216.
2.	蔡健,顧振綸,蔣小崗,等.黃芩總黃酮對博萊霉素致大鼠肺纖維化的干預作用及其機制研究.中草藥,2012,43:119-124.
3.	柴文戍,李永春,李艷勤,等.谷胱甘肽對大鼠肺纖維化模型的干預作用.中國醫師雜志,2003,5:295-297.
4.	劉瑞,李成剛,梁欣,等.博萊霉素誘導大鼠肺纖維化過程中羥脯氨酸及氧化應激指標的變化.醫學爭鳴,2005,25:2202-2205.
5.	唐仕芳,李華強,史源,等.還原型谷胱甘肽對新生鼠高氧肺纖維化早期干預的研究.重慶醫學,2005,34:1190-1191.
6.	Todd NW,Luzina IG,Atamas SP. Molecular and cellular mechanisms of pulmonary fibrosis. Fibrogenesis Tissue Repair,2012,5:11.
7.	Griese M,Kappler M,Eismann C,et al. Inhalation treatment with glutathione in patients with cystic fibrosis. A randomized clinical trial. Am J Respir Crit Care Med,2013,188:83-89.
8.	Homma S,Azuma A,Taniguchi H,et al. Efficacy of inhaled N-acetylcysteine monotherapy in patients with early stage idiopathic pulmonary fibrosis. Respirology,2012,17:467-477.
9.	呂靜. 還原型谷胱甘肽對博來霉素致大鼠肺纖維化的干預作用[學位論文].河北醫科大學,2010.
10.	劉建,劉燕梅,王玉光. 黃芪甲苷對博萊霉素誘導小鼠肺纖維化的干預作用.北京中醫藥,2015,34:484-488.
11.	李柏穎,宋康,夏永良,等.虎杖對肺纖維化大鼠肺組織中SOD、GSH、MDA的影響. 浙江醫學,2014,36:1385-1389.
12.	王笑英,韓玲,干泳華.桔梗總皂苷抗大鼠肺纖維化作用的機制.中國老年學雜志,2015,35:2957-2958.
13.	劉理靜,錢紅,張平.大黃素對肺纖維化大鼠的保護作用及部分機制研究.中國藥理學通報,2015,31:266-272.
14.	支開葉,康永,倪艷,等.肺纖方對肺纖維化大鼠肺組織自由基損傷的影響.中國中醫藥信息雜志,2012,19:32-33.

1. 支開葉,康永,倪艷,等.肺纖方干預博萊霉素致肺纖維化大鼠的實驗研究.中國實驗方劑學雜志,2012,18:212-216.
2. 蔡健,顧振綸,蔣小崗,等.黃芩總黃酮對博萊霉素致大鼠肺纖維化的干預作用及其機制研究.中草藥,2012,43:119-124.
3. 柴文戍,李永春,李艷勤,等.谷胱甘肽對大鼠肺纖維化模型的干預作用.中國醫師雜志,2003,5:295-297.
4. 劉瑞,李成剛,梁欣,等.博萊霉素誘導大鼠肺纖維化過程中羥脯氨酸及氧化應激指標的變化.醫學爭鳴,2005,25:2202-2205.
5. 唐仕芳,李華強,史源,等.還原型谷胱甘肽對新生鼠高氧肺纖維化早期干預的研究.重慶醫學,2005,34:1190-1191.
6. Todd NW,Luzina IG,Atamas SP. Molecular and cellular mechanisms of pulmonary fibrosis. Fibrogenesis Tissue Repair,2012,5:11.
7. Griese M,Kappler M,Eismann C,et al. Inhalation treatment with glutathione in patients with cystic fibrosis. A randomized clinical trial. Am J Respir Crit Care Med,2013,188:83-89.
8. Homma S,Azuma A,Taniguchi H,et al. Efficacy of inhaled N-acetylcysteine monotherapy in patients with early stage idiopathic pulmonary fibrosis. Respirology,2012,17:467-477.
9. 呂靜. 還原型谷胱甘肽對博來霉素致大鼠肺纖維化的干預作用[學位論文].河北醫科大學,2010.
10. 劉建,劉燕梅,王玉光. 黃芪甲苷對博萊霉素誘導小鼠肺纖維化的干預作用.北京中醫藥,2015,34:484-488.
11. 李柏穎,宋康,夏永良,等.虎杖對肺纖維化大鼠肺組織中SOD、GSH、MDA的影響. 浙江醫學,2014,36:1385-1389.
12. 王笑英,韓玲,干泳華.桔梗總皂苷抗大鼠肺纖維化作用的機制.中國老年學雜志,2015,35:2957-2958.
13. 劉理靜,錢紅,張平.大黃素對肺纖維化大鼠的保護作用及部分機制研究.中國藥理學通報,2015,31:266-272.
14. 支開葉,康永,倪艷,等.肺纖方對肺纖維化大鼠肺組織自由基損傷的影響.中國中醫藥信息雜志,2012,19:32-33.

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Chinese Journal of Respiratory and Critical Care Medicine

Effects of Reduced Glutathione in Rats with Pulmonary Fibrosis

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