Clinical significance of IL-17, IL-10, and 8-iso-PG in exhaled breath condensate of patients with acute exacerbation of chronic obstructive pulmonary disease_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

 YAN Binlin ¹ , GU Weili ² , DU Juan ¹ , ZHOU Huidong ¹

1. Department of Respiratory Medicine, The Seventh People’s Hospital of Shenzhen, Shenzhen, Guangdong 518081, P.R.China;
2. Department of Respiratory Medicine, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, P.R.China;

Corresponding?author：

YAN Binlin, Email: yblelite@163.com

Keywords：

Acute exacerbation of chronic obstructive pulmonary disease; Exhaled breath condensate; Interleukin-17; Interleukin-10; 8-Isoprestane

DOI：

10.7507/1671-6205.201609016

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Objective To investigate the changes and clinical significance of cytokines and inflammatory species in exhaled breath condensate (EBC) in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Methods Thirty AECOPD patients admitted in the Department of Respiratory Medicine from March 2015 to August 2016 (smokers and passive smokers) and 21 healthy volunteers (non-smokers) were recruited in this prospective study. General information and EBC were collected from each subject. The concentrations of interleukin-17 (IL-17), IL-10, and 8-isoprestane (8-iso-PG) in EBC were measured by enzyme-linked immunosorbent assay, meanwhile lung function test was performed in the AECOPD patients. Results Both IL-17 (ng/L) and 8-iso-PG (ng/L) levels increased significantly in the AECOPD patients before and after treatment compared with the healthy controls (10.74±1.02 and 5.65±0.88 vs. 3.36±0.61, 12.35±2.25 and 9.65±1.22 vs. 6.93±1.15, P<0.05). However, IL-10 level significantly decreased in the AECOPD patients before and after treatment compared with the healthy controls (1.68±0.17 and 2.59±0.31 vs. 2.85±0.43, P<0.05). Both IL-17 and 8-iso-PG levels in the AECOPD patients were significantly lower after treatment than those before treatment (5.65±0.88 vs. 10.74±1.02, 9.65±1.22 vs. 12.35±2.25, P<0.05), but IL-10 level were significantly higher aftertreatment than those before treatment (2.59±0.31 vs. 1.68±0.17, P<0.05). FEV₁, FVC, and FEV₁%pred improved significantly after treatment (P<0.01). FEV₁, FEV₁/FVC and FEV₁%pred were not significantly correlated with IL-17, IL-10 or 8-is-PG levels. Conclusion IL-17, IL-10 and 8-iso-PG may be involved in the pathogenesis of COPD, and may be important biomarkers in monitoring airway inflammation and oxide stress during the treatment of AECOPD patients.

Citation： YAN Binlin, GU Weili, DU Juan, ZHOU Huidong. Clinical significance of IL-17, IL-10, and 8-iso-PG in exhaled breath condensate of patients with acute exacerbation of chronic obstructive pulmonary disease. Chinese Journal of Respiratory and Critical Care Medicine, 2017, 16(2): 142-146. doi: 10.7507/1671-6205.201609016 Copy

1.	Melsen WG, Rovers MM, Koeman M, et al. Estimating the attributable mortality of ventilator-associated pneumonia from randomized prvention studies. Crit Care, 2011, 39(12): 2736-2742.
2.	中華醫學會呼吸病學分會慢性阻塞性肺疾病學組. 慢性阻塞性肺疾病診治指南(2013 年修訂版). 中華結核和呼吸雜志, 2013, 36(4): 255-264.
3.	Nefedov VB, Shergina EA, Popova LA. Evaluation of the course of chronic obstructive lung diseases according to the classifications of the European Respiratory Society and the Global Initiative on Chronic Obstructive Lung Disease. Probl Tuberk Bolezn Legk, 2006, (2): 44-47.
4.	劉霞, 許文兵, 劉亞男, 等. 呼出氣一氧化氮對成人支氣管哮喘治療的指導作用. 中國呼吸與危重監護雜志, 2014, 13(2): 120-124.
5.	Zhao YF, Jiang YP, Zhou LF, et al. The value of assessment tests in patients with acute exacerbation of chronic obstructive pulmonary disease. Am J Med Sci, 2014, 347(5): 393-399.
6.	Di Stefano A, Caramori G, Gnemmi I, et al. T helper type 17-related cytokine expression is increased in the bronchial mucosa of stable chronic obstructive pulmonary disease patients. Clin Exp Immunol, 2009, 157(2): 316-324.
7.	劉仁杰, 萬毅新, 王曉平, 等. 慢性阻塞性肺疾病患者血清 IL-17、IL-23 水平及臨床意義. 重慶醫科大學學報, 2011, 36(8): 976-978.
8.	萬鵬程, 關國躍. IL-17 在慢性阻塞性肺疾病發病機制中的作用. 臨床肺科雜志, 2011, 16(7): 1086-1088.
9.	何小雙, 許西琳, 劉冬, 等. 慢性阻塞性肺疾病患者呼出氣冷凝液中白介素 8、白介素 17 水平及其與第 1 秒用力呼氣末容積占預計值百分比的關系研究. 中國全科醫學, 2016, 19(1): 63-67.
10.	Tone M, Powell MJ, Tone Y, et al. IL-10 gene expression is controlled by the transcription factors Sp1 and Sp3. J Immunity, 2010, 165(1): 286-291.
11.	郝璐, 崔麗英. 支氣管哮喘和 COPD 患者血清 IL-10 水平的臨床研究. 臨床肺科雜志, 2012, 17(2): 228-230.
12.	Macdonald M, Korman T, King P, et al. Exacerbation phenotyping in chronic obstructive pulmonary disease. Respirology, 2013, 18(8): 1280 -1281.
13.	朱崢, 周奇興, 鄒盈, 等. 白介素 8 和白介素 10 在老年慢性阻塞性肺疾病誘導痰中的表達以及藥物治療對其的影響. 國際呼吸雜志, 2012, 32(7): 493-496.
14.	李小莉, 黃平, 杜秀芳. 慢性阻塞性肺疾病治療期間呼出氣冷凝液中 8-異前列腺素、IL-6、IL-10 的變化及與氣道炎癥的相關性研究. 中國呼吸與危重監護雜志, 2014, 13(5): 445-448.
15.	Davidson WJ, Verity WS, Traves SL, et al. Effect of incremental exercise on airway and systemic inflammation in patients with COPD. J Appl Physiol, 2012, 112(12): 2049-2056.
16.	Montuschi P, Barnes PJ, Roberts LJ. Isoprostanes: markers and mediators of oxidative stress. FASEB J, 2004, 18(5): 1791-1800.
17.	Crooks SW, Bayley DL, Hill SL, et al. Bronchial inflammation in acute bacterial exacerbations of chronic bronchitis: the role of leukotriene B4. Eur Respir J, 2000, 15(2): 274-280.
18.	Kostikas K, Papatheodorou G, Psathakis K, et al. Oxidative stress in expired breath condensate of patients with COPD. Chest, 2003, 124(4): 1373-1380.
19.	Rahman I, Kelly F. Biomarkers in breath condensate: a promising new non-invasive technique in free radical research. Free Radic Res, 2003, 37(12): 1253-1266.
20.	陳金亮, 陳建榮, 蔡映云, 等. COPD 患者呼出氣冷凝液中 8-異前列腺素的檢測及其意義. 臨床肺科雜志, 2011, 16(3): 341-343.
21.	郭凱, 殷少軍. 慢性阻塞性肺疾病急性加重期相關生物標志物及其診斷意義. 中華診斷學電子雜志, 2015, 3(2): 133-137.
22.	姚蘇梅, 陳金亮, 呂學東, 等. 社區獲得性肺炎患者呼出氣冷凝液和血清中 8-異前列腺素檢測的臨床意義. 檢驗醫學與臨床, 2016, 13(8): 1020-1024.
23.	李小莉, 黃平, 杜秀芳. COPD 治療期間呼出氣冷凝液中細胞因子的變化及意義. 臨床肺科雜志, 2014, 19(7): 1222-1224.

1. Melsen WG, Rovers MM, Koeman M, et al. Estimating the attributable mortality of ventilator-associated pneumonia from randomized prvention studies. Crit Care, 2011, 39(12): 2736-2742.
2. 中華醫學會呼吸病學分會慢性阻塞性肺疾病學組. 慢性阻塞性肺疾病診治指南(2013 年修訂版). 中華結核和呼吸雜志, 2013, 36(4): 255-264.
3. Nefedov VB, Shergina EA, Popova LA. Evaluation of the course of chronic obstructive lung diseases according to the classifications of the European Respiratory Society and the Global Initiative on Chronic Obstructive Lung Disease. Probl Tuberk Bolezn Legk, 2006, (2): 44-47.
4. 劉霞, 許文兵, 劉亞男, 等. 呼出氣一氧化氮對成人支氣管哮喘治療的指導作用. 中國呼吸與危重監護雜志, 2014, 13(2): 120-124.
5. Zhao YF, Jiang YP, Zhou LF, et al. The value of assessment tests in patients with acute exacerbation of chronic obstructive pulmonary disease. Am J Med Sci, 2014, 347(5): 393-399.
6. Di Stefano A, Caramori G, Gnemmi I, et al. T helper type 17-related cytokine expression is increased in the bronchial mucosa of stable chronic obstructive pulmonary disease patients. Clin Exp Immunol, 2009, 157(2): 316-324.
7. 劉仁杰, 萬毅新, 王曉平, 等. 慢性阻塞性肺疾病患者血清 IL-17、IL-23 水平及臨床意義. 重慶醫科大學學報, 2011, 36(8): 976-978.
8. 萬鵬程, 關國躍. IL-17 在慢性阻塞性肺疾病發病機制中的作用. 臨床肺科雜志, 2011, 16(7): 1086-1088.
9. 何小雙, 許西琳, 劉冬, 等. 慢性阻塞性肺疾病患者呼出氣冷凝液中白介素 8、白介素 17 水平及其與第 1 秒用力呼氣末容積占預計值百分比的關系研究. 中國全科醫學, 2016, 19(1): 63-67.
10. Tone M, Powell MJ, Tone Y, et al. IL-10 gene expression is controlled by the transcription factors Sp1 and Sp3. J Immunity, 2010, 165(1): 286-291.
11. 郝璐, 崔麗英. 支氣管哮喘和 COPD 患者血清 IL-10 水平的臨床研究. 臨床肺科雜志, 2012, 17(2): 228-230.
12. Macdonald M, Korman T, King P, et al. Exacerbation phenotyping in chronic obstructive pulmonary disease. Respirology, 2013, 18(8): 1280 -1281.
13. 朱崢, 周奇興, 鄒盈, 等. 白介素 8 和白介素 10 在老年慢性阻塞性肺疾病誘導痰中的表達以及藥物治療對其的影響. 國際呼吸雜志, 2012, 32(7): 493-496.
14. 李小莉, 黃平, 杜秀芳. 慢性阻塞性肺疾病治療期間呼出氣冷凝液中 8-異前列腺素、IL-6、IL-10 的變化及與氣道炎癥的相關性研究. 中國呼吸與危重監護雜志, 2014, 13(5): 445-448.
15. Davidson WJ, Verity WS, Traves SL, et al. Effect of incremental exercise on airway and systemic inflammation in patients with COPD. J Appl Physiol, 2012, 112(12): 2049-2056.
16. Montuschi P, Barnes PJ, Roberts LJ. Isoprostanes: markers and mediators of oxidative stress. FASEB J, 2004, 18(5): 1791-1800.
17. Crooks SW, Bayley DL, Hill SL, et al. Bronchial inflammation in acute bacterial exacerbations of chronic bronchitis: the role of leukotriene B4. Eur Respir J, 2000, 15(2): 274-280.
18. Kostikas K, Papatheodorou G, Psathakis K, et al. Oxidative stress in expired breath condensate of patients with COPD. Chest, 2003, 124(4): 1373-1380.
19. Rahman I, Kelly F. Biomarkers in breath condensate: a promising new non-invasive technique in free radical research. Free Radic Res, 2003, 37(12): 1253-1266.
20. 陳金亮, 陳建榮, 蔡映云, 等. COPD 患者呼出氣冷凝液中 8-異前列腺素的檢測及其意義. 臨床肺科雜志, 2011, 16(3): 341-343.
21. 郭凱, 殷少軍. 慢性阻塞性肺疾病急性加重期相關生物標志物及其診斷意義. 中華診斷學電子雜志, 2015, 3(2): 133-137.
22. 姚蘇梅, 陳金亮, 呂學東, 等. 社區獲得性肺炎患者呼出氣冷凝液和血清中 8-異前列腺素檢測的臨床意義. 檢驗醫學與臨床, 2016, 13(8): 1020-1024.
23. 李小莉, 黃平, 杜秀芳. COPD 治療期間呼出氣冷凝液中細胞因子的變化及意義. 臨床肺科雜志, 2014, 19(7): 1222-1224.

Chinese Journal of Respiratory and Critical Care Medicine

Clinical significance of IL-17, IL-10, and 8-iso-PG in exhaled breath condensate of patients with acute exacerbation of chronic obstructive pulmonary disease

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