The Effect of Medical Ozone on Lung Tissue of Pulmonary Fibrosis Rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

ZhaoShunxin ,  LiuJianbo

Department of Respiratory Medicine, The Second Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, 450000, China;

Corresponding?author：

LiuJianbo, Email: jbliuzz@163.com

Keywords：

Pulmonary fibrosis; Medical ozone; Superoxide dismutase; Hydroxyproline

DOI：

10.7507/1671-6205.2016010

Video：

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Objective To explore the feasibility of medical ozone in treatment of pulmonary fibrosis. Methods Forty Wistar rats were randomly divided into an experimental group and a control group, with 20 rats in each group.All rats were intratreacheally instilled with bleomycin to induce pulmonary fibrosis.Then the rats were intraperitoneally injected with physiological saline every other day in the control group, and with medical ozone every other day in the experimental group.After 28 days, 10 rats in each group were sacrificed after lung function test.Right lung tissues were sampled for pathological examination, and left lung tissues were sampled for measurement of superoxide dismutase (SOD) and hydroxyproline.The remaining 10 rats in each group continued to be normally fed and intraperitoneally injected for observation of the survival time. Results The lung function of the control group significantly decreased compared with the experimental group.The degree of lung fibrosis in the control group was more severe than that in the experimental group (lung fibrosis score: 1.9±0.5 vs.1.2±0.4, P < 0.05). The level of SOD in lung tissue was significantly higher and the level of hydroxyproline was significantly lower in the experimental group compared with the control group [(208.48±29.37)U·mg^-1·pro^-1 vs.(163.34±21.42) U·mg^-1·pro^-1, (2.25±0.28) mg/g vs.(2.68±0.37) mg/g, P < 0.05].The rats in the experimental group had longer survival time compared with the control group (79 d vs.59 d, P < 0.05). Conclusion Medical ozone can delay the progress of pulmonary fibrosis in rats.

Citation： ZhaoShunxin, LiuJianbo. The Effect of Medical Ozone on Lung Tissue of Pulmonary Fibrosis Rats. Chinese Journal of Respiratory and Critical Care Medicine, 2016, 15(1): 39-42. doi: 10.7507/1671-6205.2016010 Copy

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2.	蔡后榮.2011年特發性肺纖維化診斷和治療循證新指南解讀.中國呼吸與危重監護雜志, 2011, 10:313-316.
3.	黃霞, 劉惠霞, 孫為, 等.不同治則對肺間質纖維化大鼠氧自由基損傷的干預作用.中國實驗方劑學雜志, 2010, 16:188-191.
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7.	Zeng M, Liao B, Zhu C, et al.Aerosolized STAT1 antisense oligodeoxynucleotides decrease the concentrations of inflammatory mediators in bronchoalveolar lavage fluid in bleomycin-induced rat pulmonary fibrosis.Cell Mol Immunol, 2008:219-224.
8.	Long X, Xiong SD, Xiong WN, et al. Effect of intramuscular injection of hepatocyte growth factor plasmid DNA with electroporation on bleomycin-induced lung fibrosis in rats.Clin Med J(Engl), 2007, 120:1432-1437.
9.	陳衛巧, 杜國平.醫用臭氧安全性的研究進展.中國醫學創新, 2015, 12:147-149.
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11.	李惠萍, 王思勤, 周建英, 等.國產吡非尼酮治療特發性肺纖維化Ⅱ期臨床研究.中國呼吸與危重監護雜志, 2015, 14:229-235.
12.	Oruqaj G, Karnati S, Vijayan V, et al.Compromised peroxisomes in idiopathic pulmonary fibrosis, a vicious cycle inducing a higher fibrotic response via TGF-βsignaling.Proc Natl Acad Sci USA, 2015, 112:E2048-E2057.
13.	Fattman CL, Schaefer LM, Oury TD.Extracellular superoxide dismutase in biology and medicine.Free Radic Biol Med, 2003, 35:236-256.
14.	Cooper AD. Pulmonary fibrosis:pathways are slowly coming into light.Am J Respir Cell Mol Biol, 2000, 22:520-523.
15.	Larini A, Bianchi L, Bocci V. The ozone to leranc:enhancement of antioxidant enzymes is ozone dose dependent in Jurkat cells.Free Radic Res, 2003, 37:1163-1168.
16.	Russell PB, James DC. Oxidative stress in airways:is there a role for extracellular superoxide dismutase.Am J Respir Crit Care Med, 2002, 166:38-43.
17.	邱勤業, 余斌.醫用臭氧對金黃色葡萄球菌和大腸埃希菌的殺滅作用.南方醫科大學學報, 2010, 30:929-930.

1. 葛均波, 徐永健, 等.內科學.第8版.北京:人民衛生出版社, 2013, 89-92.
2. 蔡后榮.2011年特發性肺纖維化診斷和治療循證新指南解讀.中國呼吸與危重監護雜志, 2011, 10:313-316.
3. 黃霞, 劉惠霞, 孫為, 等.不同治則對肺間質纖維化大鼠氧自由基損傷的干預作用.中國實驗方劑學雜志, 2010, 16:188-191.
4. Tanjore H, Blackwell TS, Lawson WE. Emerging evidence for endoplasmic reticulum stress in the pathogenesis of idiopathic pulmonary fibrosis.Am J Physiol Lung Cell Mol Physiol, 2012, 302:L721-L729.
5. Peralta C, Bulbena O, Xaus C.Ischemic preconditioning:a defense mechanism against the reactive oxygen species generated after hepatic ischemia reperfusion. Transplantation, 2002, 73:1203-1207.
6. Hagiwara SI, Ishii Y, Kitamura S.Aerosolized administration of N-acetylcysteine allenuates lung fibrosis induced by bleomycin in mice. Am J Respir Crit Care Med, 2000, 162:225-230.
7. Zeng M, Liao B, Zhu C, et al.Aerosolized STAT1 antisense oligodeoxynucleotides decrease the concentrations of inflammatory mediators in bronchoalveolar lavage fluid in bleomycin-induced rat pulmonary fibrosis.Cell Mol Immunol, 2008:219-224.
8. Long X, Xiong SD, Xiong WN, et al. Effect of intramuscular injection of hepatocyte growth factor plasmid DNA with electroporation on bleomycin-induced lung fibrosis in rats.Clin Med J(Engl), 2007, 120:1432-1437.
9. 陳衛巧, 杜國平.醫用臭氧安全性的研究進展.中國醫學創新, 2015, 12:147-149.
10. Szapiel SV, Elson NA, Fulmer JD, et al. Bleomycin-induced interstitial pulmonary disease in the nude, athymic mouse.Am Rev Respir Dis, 1979, 120:893-899.
11. 李惠萍, 王思勤, 周建英, 等.國產吡非尼酮治療特發性肺纖維化Ⅱ期臨床研究.中國呼吸與危重監護雜志, 2015, 14:229-235.
12. Oruqaj G, Karnati S, Vijayan V, et al.Compromised peroxisomes in idiopathic pulmonary fibrosis, a vicious cycle inducing a higher fibrotic response via TGF-βsignaling.Proc Natl Acad Sci USA, 2015, 112:E2048-E2057.
13. Fattman CL, Schaefer LM, Oury TD.Extracellular superoxide dismutase in biology and medicine.Free Radic Biol Med, 2003, 35:236-256.
14. Cooper AD. Pulmonary fibrosis:pathways are slowly coming into light.Am J Respir Cell Mol Biol, 2000, 22:520-523.
15. Larini A, Bianchi L, Bocci V. The ozone to leranc:enhancement of antioxidant enzymes is ozone dose dependent in Jurkat cells.Free Radic Res, 2003, 37:1163-1168.
16. Russell PB, James DC. Oxidative stress in airways:is there a role for extracellular superoxide dismutase.Am J Respir Crit Care Med, 2002, 166:38-43.
17. 邱勤業, 余斌.醫用臭氧對金黃色葡萄球菌和大腸埃希菌的殺滅作用.南方醫科大學學報, 2010, 30:929-930.

Chinese Journal of Respiratory and Critical Care Medicine

The Effect of Medical Ozone on Lung Tissue of Pulmonary Fibrosis Rats

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