Effects of Smoking on Th17/Treg T Cell Subsets and Cytokines Expression in Patients with Chronic Obstructive Pulmonary Disease in Stable Phase_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

XuDan ¹ , GongLi ² , XuQian ¹ , WangJing ¹ , JiangMin ¹ , GaoZhen ¹ ,  LiFengsen ²

1. The COPD Lab of National Clinical Research Base of Traditional Chinese Medicine, Urumqi, Xinjiang, 830000, China;
2. ;

Corresponding?author：

LiFengsen, Email: fengsen602@163.com

Keywords：

Chronic obstructive pulmonary disease; Smoking; Th17/Treg; Cytokines

DOI：

10.7507/1671-6205.2015003

Video：

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Objective To investigate the effects of smoking on Th17/Treg T cell subsets and cytokines expression in patients with chronic obstructive pulmonary disease (COPD) in stable stage. Methods From February 2012 to June 2013, sixty outpatients with stable COPD (20 cases of non-smokers, 40 cases of smokers) and 15 normal volunteers were recruited in the study in the Traditional Chinese Medicine Hospital affiliated to Xinjiang Medical University. Th17/Treg level in peripheral blood was detected by flow cytometry method. Cytometric bead array system was used to detect TGF-β, IL-10, IL-17A, IFN-γand other inflammatory factors in serum. Results The patients' age, duration of disease, lung function, disease severity, and other related data were comparable between the smoking COPD group and the non-smoking COPD group (P > 0.05). Th17/Treg level was increased in the smoking COPD group compared with the normal group (P < 0.05), and showed an increasing trend from the normal group to the non-smoking COPD group and the smoking COPD group. The level of IL-2 in the smoking and non-smoking COPD groups was lower than that in the normal group. Compared with the normal group, the level of TNF-αwas significantly decreased in the smoking and non-smoking COPD groups(P < 0.05). Conclusions Systemic inflammatory response continuously exists in patients with COPD even in the stable phase. Smoking can partly enhance the inflammatory reaction in COPD. The Th17/Treg T cell subsets associated cytokine regulation has gradually tended to a balance in the stable phase, and inflammatory factors related recovery speed is not consistent, suggesting that smoking may play a certain role in the recovery of balance.

Citation： XuDan, GongLi, XuQian, WangJing, JiangMin, GaoZhen, LiFengsen. Effects of Smoking on Th17/Treg T Cell Subsets and Cytokines Expression in Patients with Chronic Obstructive Pulmonary Disease in Stable Phase. Chinese Journal of Respiratory and Critical Care Medicine, 2015, 14(1): 5-9. doi: 10.7507/1671-6205.2015003 Copy

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14.	楊玉書, 劉士林.吸煙與肺部感染在慢性阻塞性肺部疾病中的發病機理探討.臨床肺科雜志, 2009, 14:796-797.
15.	Adair-Kirk TL, Atkinson JJ, Senior RM.Smoke particulates stress lung cells.Nat Med, 2008, 14:1024-1025.
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17.	姜敏, 田戈, 王晶, 等.維藥神香草對COPD小鼠Th1/Th2和Th17/Treg平衡的影響.世界科學技術-中醫藥現代化, 2013, 15:591-594.
18.	王勝軍, 許化溪, 楊勝利.CD4⁺CD25⁺調節性T細胞.細胞生物學雜志, 2005, 27:61-65.
19.	Li MO, Wan YY, Flavell RA.T cell-produced transforming growth factorβ1 controls T cell tolerance and regulates Th1-and Th17-cell differentiation.Immunity, 2007, 26:579-591.

1. 任玲, 朱蘭, 王長捷.健康老年人血脂與年齡、性別的關系.現代預防醫學, 2003, 30:532-533.
2. L?kke A, Lange P, Scharling H, et al.Developing COPD:a 25 year follow up study of the general population.Thorax, 2006, 61:935-939.
3. 駱樹新, 許西琳, 關鍵.吸煙與慢性阻塞性肺病肺功能相關性研究.中華實用診斷與治療雜志, 2011, 25:621-622.
4. Harrington LE, Mangan PR, Weaver CT.Expanding the effector CD4 T-cell repertoire:the Th17 lineage.Curr Opin Immunol, 2006, 18:349-56.
5. 李鴻佳, 蓋慶玲, 王立華, 等.Th17淋巴細胞在哮喘小鼠氣道炎癥中的初步研究.細胞與分子免疫學雜志, 2011, 27:53-55.
6. Annunziato F, Cosmi L, SantarlasciV, et al.Phenotypic and functional features of human Th17 cells.J Exp Med, 2007, 204:1849-1861.
7. 劉秀榮, 李玉青, 張朱佳子, 等.北京市疾病預防控制中心系統控煙現狀調查.疾病監測, 2009, 24:57-59.
8. Higashimoto Y, Yamagata Y, Taya S, et al.Systemic inflammation in chronic obstructive pulmonary disease and asthma:similarities and differences.Respirology, 2008, 13:128-133.
9. Vemooy JH, Kucukaycan M, Jacobs JA, et al.Local and systemic inflammation in patients with chronic obstructive pulmonary disease.Am J Respir Crit Care Med, 2002, 166:1218-1224.
10. 程霞.COPD穩定期患者血清IL-6、IL-8、hs-CRP水平與吸煙關系探討.中國臨床研究, 2010, 23:273-274.
11. Fletcher C, Peto R.The natural history of chronic airflow obstruction.Br Med J, 1977, 1:1645-1648.
12. Global strategy for the diagnosis, management and prevention of chronic obstructive pulmonary disease (revised 2011)[EB/OL].http://www.goldcopd.org/guidelines-pocket-guide-to-copd-diagnosis.html.
13. Gamble E, Grootendorst DC, Hattotuwa K, et al.Airway mucosal inflammation in COPD is similar in smokers and ex-smokers:a pooled analysis.Eur Respir J, 2007, 30:467-471.
14. 楊玉書, 劉士林.吸煙與肺部感染在慢性阻塞性肺部疾病中的發病機理探討.臨床肺科雜志, 2009, 14:796-797.
15. Adair-Kirk TL, Atkinson JJ, Senior RM.Smoke particulates stress lung cells.Nat Med, 2008, 14:1024-1025.
16. Mangan PR, Harrington IE, Quinn DB, et al.Transforming growth factor-beta induces development of the TH17 Lineage.Nature, 2006, 441:231-234.
17. 姜敏, 田戈, 王晶, 等.維藥神香草對COPD小鼠Th1/Th2和Th17/Treg平衡的影響.世界科學技術-中醫藥現代化, 2013, 15:591-594.
18. 王勝軍, 許化溪, 楊勝利.CD4⁺CD25⁺調節性T細胞.細胞生物學雜志, 2005, 27:61-65.
19. Li MO, Wan YY, Flavell RA.T cell-produced transforming growth factorβ1 controls T cell tolerance and regulates Th1-and Th17-cell differentiation.Immunity, 2007, 26:579-591.

Chinese Journal of Respiratory and Critical Care Medicine

Effects of Smoking on Th17/Treg T Cell Subsets and Cytokines Expression in Patients with Chronic Obstructive Pulmonary Disease in Stable Phase

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