The Effect of Naringenin on Vascular Endothelial Growth Factor Released by Human Fetal Lung Fibroblasts_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

XuXiantao ,  SunLihua , ShiYing

Nanjing Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, 210006, China;

Corresponding?author：

SunLihua, Email: nhfsun@sina.com

Keywords：

Naringenin; Cigarette smoke extract; Vascular endothelial growth factor; Smad3

DOI：

10.7507/1671-6205.2014005

Video：

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Objective To explore the effect of naringenin on the expression of vascular endothelial growth factor (VEGF) released by human fetal lung fibroblasts. Methods Human fetal lung fibroblast cells were divided into a control group,a cigarette smoke extract (CSE) group and a naringenin group. Cells in the naringenin group were incubated with different doses of naringenin for 2h. Then the naringenin group and the CSE group were incubated with CSE to adjust the final concentration of naringenin (5 μmol/L,10 μmol/L,and 20 μmol/L) and of CSE(5%). The concentration of VEGF was measured in human fetal lung fibroblasts after cultured for 24h,48h and 72h by enzyme-linked immunoabsorbent assay. Smad3 and p-Smad3 levels were detected by Western blot. Results ELISA results showed that the CSE can significantly increase the VEGF expression,and naringenin can inhibit the increasing of the VEGF expression. Western blot results showed that the CSE can increase the p-Smad3 expression,and the naringenin can inhibit the increasing of the p-Smad3 expression. Conclusion Naringenin can inhibit phosphorylation of Smad3,and decrease the expression of VEGF released by human fetal lung fibroblasts.

Citation： XuXiantao, SunLihua, ShiYing. The Effect of Naringenin on Vascular Endothelial Growth Factor Released by Human Fetal Lung Fibroblasts. Chinese Journal of Respiratory and Critical Care Medicine, 2014, 13(1): 18-20. doi: 10.7507/1671-6205.2014005 Copy

1.	中華醫學會呼吸病學分會慢性阻塞性肺疾病學組.慢性阻塞性肺疾病診治指南(2007年修訂版).中華內科雜志,2007,46:254-261.
2.	Papaioannou AI, Kostikas K, Kollia P, et al. Clinical implications for vascular endothelial growth factor in the lung:friend or foe? Respir Res,2006,7:128.
3.	Farid M,Kanaji N,Nakanishi M,Gunji Y,et al.Smad3 mediates cigarette smoke extract (CSE) induction of VEGF release by human fetal lung fibroblasts.Toxicol Lett,2013,220:126-134.
4.	H?m?l?inen M,Nieminen R,Vuorela P,et al.Anti-inflammatory effects of flavonoids:genistein,kaempferol,quercetin,and daidzein inhibit STAT-1 and NF-kappaB activations,whereas flavone,isorhamnetin,naringenin,and pelargonidin inhibit only NF-kappaB activation along with their inhibitory effect on iNOS expression and NO production in activated macrophages. Mediators Inflamm,2007,45673.
5.	Kanno S,Tomizawa A,Hiura T,et al.Inhibitory effects of naringenin on tumor growth in human cancer cell lines and sarcoma S-180-implanted mice.Biol Pharm Bull,2005,28:527-530.
6.	Zielińska-Przyjemska M,Ignatowicz E.Citrus fruit flavonoids influence on neutrophil apoptosis and oxidative metabolism.Phytother Res,2008,22:1557-1562.
7.	Hsiao YC,Kuo WH,Chen PN,et al.Flavanone and 2'-OH flavanone inhibit metastasis of lung cancer cells via down-regulation of proteinases activities and MAPK pathway.Chem Biol Interact,2007,167:193-206.
8.	Cayci C,Wahlquist TC,Seckin SI,et al.Naringenin inhibits neointimal hyperplasia following arterial reconstruction with interpositional vein graft.Ann Plast Surg,2010,64:105-113.
9.	Yunchao S,Weihong H,Carlos G,et al.Effect of cigarette smoke extract on nitric oxide synthase in pulmonary artery endothelial cells.Am J Respir Cell Mol Biol,1998,19:819-825.
10.	Kobayashi T,Liu X,Wen FQ,et al.Smad3 mediates TGF-beta1 induction of VEGF production in lung fibroblasts.Biochem Biophys Res Commun,2005,327:393-398.

1. 中華醫學會呼吸病學分會慢性阻塞性肺疾病學組.慢性阻塞性肺疾病診治指南(2007年修訂版).中華內科雜志,2007,46:254-261.
2. Papaioannou AI, Kostikas K, Kollia P, et al. Clinical implications for vascular endothelial growth factor in the lung:friend or foe? Respir Res,2006,7:128.
3. Farid M,Kanaji N,Nakanishi M,Gunji Y,et al.Smad3 mediates cigarette smoke extract (CSE) induction of VEGF release by human fetal lung fibroblasts.Toxicol Lett,2013,220:126-134.
4. H?m?l?inen M,Nieminen R,Vuorela P,et al.Anti-inflammatory effects of flavonoids:genistein,kaempferol,quercetin,and daidzein inhibit STAT-1 and NF-kappaB activations,whereas flavone,isorhamnetin,naringenin,and pelargonidin inhibit only NF-kappaB activation along with their inhibitory effect on iNOS expression and NO production in activated macrophages. Mediators Inflamm,2007,45673.
5. Kanno S,Tomizawa A,Hiura T,et al.Inhibitory effects of naringenin on tumor growth in human cancer cell lines and sarcoma S-180-implanted mice.Biol Pharm Bull,2005,28:527-530.
6. Zielińska-Przyjemska M,Ignatowicz E.Citrus fruit flavonoids influence on neutrophil apoptosis and oxidative metabolism.Phytother Res,2008,22:1557-1562.
7. Hsiao YC,Kuo WH,Chen PN,et al.Flavanone and 2'-OH flavanone inhibit metastasis of lung cancer cells via down-regulation of proteinases activities and MAPK pathway.Chem Biol Interact,2007,167:193-206.
8. Cayci C,Wahlquist TC,Seckin SI,et al.Naringenin inhibits neointimal hyperplasia following arterial reconstruction with interpositional vein graft.Ann Plast Surg,2010,64:105-113.
9. Yunchao S,Weihong H,Carlos G,et al.Effect of cigarette smoke extract on nitric oxide synthase in pulmonary artery endothelial cells.Am J Respir Cell Mol Biol,1998,19:819-825.
10. Kobayashi T,Liu X,Wen FQ,et al.Smad3 mediates TGF-beta1 induction of VEGF production in lung fibroblasts.Biochem Biophys Res Commun,2005,327:393-398.

Chinese Journal of Respiratory and Critical Care Medicine

The Effect of Naringenin on Vascular Endothelial Growth Factor Released by Human Fetal Lung Fibroblasts

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