Experimental study of interferon-γ in preventing intestinal adhesion following abdominal surgery in rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Shengbi ¹ , LI Xiangtao ¹ , ZHOU Jinhong ² , ZHAO Qi ² ,  WENG Qifang ³

1. Grade 2014 Clinical Medicine, Hainan Medical College, Haikou 571199, P. R. China;
2. Grade 2015 Clinical Medicine, Hainan Medical College, Haikou 571199, P. R. China;
3. Department of Physiology, Hainan Medical College, Haikou 571199, P. R. China;

Corresponding?author：

WENG Qifang, Email: 33201109@qq.com

Keywords：

interferon γ; fibroblast; interleukin 13; intestinal adhesion

DOI：

10.7507/1007-9424.201810011

Video：

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Objective To observe effect of interferon-γ in preventing intestinal adhesion following abdominal surgery in rats.Methods A total of 60 Wistar rats were selected, which were randomly divided into a sham operation (SO) group, model group, dexamethasone (DXMS) group, and interferon-γ group, then the interferon-γ group was randomly divided into a low, medium, and high concentrations subgroups. Except for the SO group, the laparotomies with file friction caecum were performed for all the other groups to establish the intestinal adhesion model. SO group and model group were intraperitoneally injected with saline for 4 mL/kg. The 10 mg/kg dexamethasone was injected into the abdominal cavity of rats in the DXMS group. The 7.5×10⁴, 1.5×10⁵, and 3.0×10⁵ U/kg interferon-γ concentrations were injected into the abdominal cavity of rats in the low, medium, and high concentrations subgroups respectively. The Nair grading of cecum adhesion degree was assessed on the 8th day after the surgery, then the histopathological change was observed by the HE staining under the microscopy and the hydroxyproline content in the cecum tissue was detected.Results ① The intestinal adhesion: Compared with the SO group, the intestinal adhesions occurred in all the other groups and the degrees of intestinal adhesions evaluated by the Nair grading were more significantly serious (P<0.05), which in the DXMS group and the medium and high concentrations of interferon-γ subgroups were significantly reduced (P<0.05) as compared with the model group, which in the high concentration of interferon-γ subgroup was significantly reduced (P<0.05) as compared with the DXMS group. ② The microscopic observation of histopathological results: Compared with the DXMS group, the high concentration of interferon-γ could effectively reduce the occurrence of fibrous tissue and inflammatory cell infiltration, the intestinal wall muscular layer structure was complete, a few inflammatory cells scattered in the infiltration. ③ The hydroxyproline content: The contents of hydroxyproline in the cecum tissue of the model group, DXMS group, and interferon-γ subgroups were significantly increased (P<0.05) as compared with the SO group, which of the DXMS group and medium and high concentrations of interferon-γ subgroups were significantly decreased (P<0.05) as compared with the model group, which of the high concentration of interferon-γ subgroup was significantly decreased (P<0.05) as compared with the DXMS group.Conclusion Interferon-γ has a preventive and therapeutic effect on postoperative intestinal adhesion and cecum injuries.

Citation： LI Shengbi, LI Xiangtao, ZHOU Jinhong, ZHAO Qi, WENG Qifang. Experimental study of interferon-γ in preventing intestinal adhesion following abdominal surgery in rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2019, 26(1): 19-24. doi: 10.7507/1007-9424.201810011 Copy

1.	劉國濤, 陳巧, 金麗明, 等. 丹參多酚酸鹽聯合聚維酮碘預防大鼠術后腸粘連. 浙江中西醫結合雜志, 2015, 25(6): 538-541.
2.	Bianchi E, Boekelheide K, Sigman M, et al. Ghrelin inhibits post-operative adhesions via blockage of the TGF-β signaling pathway. PLoS One, 2016, 11(4): e0153968.
3.	李克躍, 石承先, 湯可立, 等. 白細胞介素13對膽管成纖維細胞TGF-β1/Smads通路表達的影響及地塞米松的干預作用. 中國普通外科雜志, 2017, 26(8): 987-992.
4.	Du XH, Liu JQ, Xin K, et al. Dexamethasone and sodium carboxymethyl cellulose prevent postoperative intraperitoneal adhesions in rats. Braz J Med Biol Res, 2015, 48(4): 344-348.
5.	Li K, Shi C, Huang J, et al. Dexamethasone effect on the expression of transforming growth factor-β1/Smads signaling pathway in benign biliary stricture fibroblasts in rodent. J Coll Physicians Surg Pak, 2017, 27(3): 131-134.
6.	熊麗霞, 李文林, 蔡震宇, 等. 干擾素γ抑制IL-13對成纖維細胞的纖維化作用. 中國病理生理雜志, 2013, 29(3): 504-508.
7.	Lalountas M, Ballas KD, Michalakis A, et al. Postoperative adhesion prevention using a statin-containing cellulose film in an experimental model. Br J Surg, 2012, 99(3): 423-429.
8.	Morello S, Southwood LL, Engiles J, et al. Effect of intraperitoneal PERIDAN? concentrate adhesion reduction device on clinical findings, infection, and tissue healing in an adult horse jejunojejunostomy model. Vet Surg, 2012, 41(5): 568-581.
9.	Yu L, Hu H, Chen L, et al. Comparative studies of thermogels in preventing postoperative adhesions and corresponding mechanisms. Biomater Sci, 2014, 2(8): 1100-1109.
10.	Nair SK, Bhat IK, Aurora AL. Role of proteolytic enzyme in the prevention of postoperative intraperitoneal adhesions. Arch Surg, 1974, 108(6): 849-853.
11.	劉勇, 夏軍強, 張琴. 腹腔鏡直腸癌經腹會陰聯合切除術中盆底腹膜重建對術后并發癥的影響. 中國普外基礎與臨床雜志, 2016, 23(11): 1375-1378.
12.	馬麗桃, 張二帥, 車軒, 等. 改性木葡聚糖溫敏水凝膠預防大鼠腹腔粘連的實驗研究. 廣東醫學, 2016, 37(21): 3184-3188.
13.	Lin LX, Yuan F, Zhang HH, et al. Evaluation of surgical anti-adhesion products to reduce postsurgical intra-abdominal adhesion formation in a rat model. PLoS One, 2017, 12(2): e0172088.
14.	Firszt R, Francisco D, Church TD, et al. Interleukin-13 induces collagen type-1 expression through matrix metalloproteinase-2 and transforming growth factor-β1 in airway fibroblasts in asthma. Eur Respir J, 2014, 43(2): 464-473.
15.	毛春芹, 曾莉, 陸兔林, 等. 川芎嗪納米噴霧劑對大鼠腹腔粘連相關因子和纖溶系統影響的實驗研究. 中國藥理學通報, 2012, 28(1): 118-123.
16.	Saleiro D, Blyth GT, Kosciuczuk EM, et al. IFN-γ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5. Sci Signal, 2018, 11(557): pii: eaap9921.
17.	高曉方, 崔社懷. 干擾素γ對實驗大鼠肺纖維化的治療作用及機制的初步研究. 第三軍醫大學學報, 2005, 27(2): 143-145.
18.	李璐, 王繼華, 趙亞南, 等. IFN-γ對大鼠創面愈合中Smad7表達的影響. 中國美容醫學, 2009, 18(2): 201-204.
19.	朱松濤. 干擾素γ聯合坎地沙坦治療肝纖維化的近期療效觀察. 中華全科醫學, 2010, 8(10): 1268-1269.
20.	熊麗霞, 石小玉. IL-13對成纖維細胞膠原蛋白合成的影響及其分子調控機制. 南昌大學, 2007.
21.	Ramalingam TR, Gieseck RL, Acciani TH, et al. Enhanced protection from fibrosis and inflammation in the combined absence of IL-13 and IFN-γ. J Pathol, 2016, 239(3): 344-354.
22.	Lu J, Zhu Y, Feng W, et al. Platelet-derived growth factor mediates interleukin-13-induced collagen Ⅰ production in mouse airway fibroblasts. J Biosci, 2014, 39(4): 693-700.
23.	李雪梅, 翟麗東. 干擾素γ與甲強龍聯合應用對人胚肺成纖維細胞增殖的影響. 中國組織工程研究與臨床康復, 2008, 12(15): 2829-2833.
24.	王敏, 梁航, 王麗娜, 等. 干擾素γ治療毛細支氣管炎的療效觀察. 中國生物制品學雜志, 2012, 25(2): 228-229, 236.
25.	崔娟娟, 馮占芹, 張守強, 等. 羧甲基殼聚糖沖洗液預防大鼠術后腹膜粘連. 中國組織工程研究, 2014, 18(8): 1250-1256.

1. 劉國濤, 陳巧, 金麗明, 等. 丹參多酚酸鹽聯合聚維酮碘預防大鼠術后腸粘連. 浙江中西醫結合雜志, 2015, 25(6): 538-541.
2. Bianchi E, Boekelheide K, Sigman M, et al. Ghrelin inhibits post-operative adhesions via blockage of the TGF-β signaling pathway. PLoS One, 2016, 11(4): e0153968.
3. 李克躍, 石承先, 湯可立, 等. 白細胞介素13對膽管成纖維細胞TGF-β1/Smads通路表達的影響及地塞米松的干預作用. 中國普通外科雜志, 2017, 26(8): 987-992.
4. Du XH, Liu JQ, Xin K, et al. Dexamethasone and sodium carboxymethyl cellulose prevent postoperative intraperitoneal adhesions in rats. Braz J Med Biol Res, 2015, 48(4): 344-348.
5. Li K, Shi C, Huang J, et al. Dexamethasone effect on the expression of transforming growth factor-β1/Smads signaling pathway in benign biliary stricture fibroblasts in rodent. J Coll Physicians Surg Pak, 2017, 27(3): 131-134.
6. 熊麗霞, 李文林, 蔡震宇, 等. 干擾素γ抑制IL-13對成纖維細胞的纖維化作用. 中國病理生理雜志, 2013, 29(3): 504-508.
7. Lalountas M, Ballas KD, Michalakis A, et al. Postoperative adhesion prevention using a statin-containing cellulose film in an experimental model. Br J Surg, 2012, 99(3): 423-429.
8. Morello S, Southwood LL, Engiles J, et al. Effect of intraperitoneal PERIDAN? concentrate adhesion reduction device on clinical findings, infection, and tissue healing in an adult horse jejunojejunostomy model. Vet Surg, 2012, 41(5): 568-581.
9. Yu L, Hu H, Chen L, et al. Comparative studies of thermogels in preventing postoperative adhesions and corresponding mechanisms. Biomater Sci, 2014, 2(8): 1100-1109.
10. Nair SK, Bhat IK, Aurora AL. Role of proteolytic enzyme in the prevention of postoperative intraperitoneal adhesions. Arch Surg, 1974, 108(6): 849-853.
11. 劉勇, 夏軍強, 張琴. 腹腔鏡直腸癌經腹會陰聯合切除術中盆底腹膜重建對術后并發癥的影響. 中國普外基礎與臨床雜志, 2016, 23(11): 1375-1378.
12. 馬麗桃, 張二帥, 車軒, 等. 改性木葡聚糖溫敏水凝膠預防大鼠腹腔粘連的實驗研究. 廣東醫學, 2016, 37(21): 3184-3188.
13. Lin LX, Yuan F, Zhang HH, et al. Evaluation of surgical anti-adhesion products to reduce postsurgical intra-abdominal adhesion formation in a rat model. PLoS One, 2017, 12(2): e0172088.
14. Firszt R, Francisco D, Church TD, et al. Interleukin-13 induces collagen type-1 expression through matrix metalloproteinase-2 and transforming growth factor-β1 in airway fibroblasts in asthma. Eur Respir J, 2014, 43(2): 464-473.
15. 毛春芹, 曾莉, 陸兔林, 等. 川芎嗪納米噴霧劑對大鼠腹腔粘連相關因子和纖溶系統影響的實驗研究. 中國藥理學通報, 2012, 28(1): 118-123.
16. Saleiro D, Blyth GT, Kosciuczuk EM, et al. IFN-γ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5. Sci Signal, 2018, 11(557): pii: eaap9921.
17. 高曉方, 崔社懷. 干擾素γ對實驗大鼠肺纖維化的治療作用及機制的初步研究. 第三軍醫大學學報, 2005, 27(2): 143-145.
18. 李璐, 王繼華, 趙亞南, 等. IFN-γ對大鼠創面愈合中Smad7表達的影響. 中國美容醫學, 2009, 18(2): 201-204.
19. 朱松濤. 干擾素γ聯合坎地沙坦治療肝纖維化的近期療效觀察. 中華全科醫學, 2010, 8(10): 1268-1269.
20. 熊麗霞, 石小玉. IL-13對成纖維細胞膠原蛋白合成的影響及其分子調控機制. 南昌大學, 2007.
21. Ramalingam TR, Gieseck RL, Acciani TH, et al. Enhanced protection from fibrosis and inflammation in the combined absence of IL-13 and IFN-γ. J Pathol, 2016, 239(3): 344-354.
22. Lu J, Zhu Y, Feng W, et al. Platelet-derived growth factor mediates interleukin-13-induced collagen Ⅰ production in mouse airway fibroblasts. J Biosci, 2014, 39(4): 693-700.
23. 李雪梅, 翟麗東. 干擾素γ與甲強龍聯合應用對人胚肺成纖維細胞增殖的影響. 中國組織工程研究與臨床康復, 2008, 12(15): 2829-2833.
24. 王敏, 梁航, 王麗娜, 等. 干擾素γ治療毛細支氣管炎的療效觀察. 中國生物制品學雜志, 2012, 25(2): 228-229, 236.
25. 崔娟娟, 馮占芹, 張守強, 等. 羧甲基殼聚糖沖洗液預防大鼠術后腹膜粘連. 中國組織工程研究, 2014, 18(8): 1250-1256.

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Experimental study of interferon-γ in preventing intestinal adhesion following abdominal surgery in rats

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