Expression of long non-coding RNA GAS5 in hepatocellular carcinoma tissues and its clinical significance_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

QIU Jianguo , SHI Zhengrong ,  LI Ming

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P. R. China;

Corresponding?author：

LI Ming, Email: limingcyfyy945@163.com

Keywords：

hepatocellular carcinoma; growth arrest-specific 5; long non-coding RNA; prognosis

DOI：

10.7507/1007-9424.201807021

Video：

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Objective To investigate the expression of growth arrest-specific 5 (GAS5) mRNA and its clinical significance in hepatocellular carcinoma.Methods The expression of GAS5 mRNA in the hepatocellular carcinoma tissues and corresponding adjacent tissues were detected by real time-PCR. The relationship between the expression of GAS5 mRNA and clinicopathological characteristics were analyzed by SPSS 19.0 software.Results The expression of GAS5 mRNA in hepatocellular carcinoma tissues was significantly lower than that of the adjacent tissues (P<0.01). The expression of GAS5 mRNA was related to tumor size, tumor number, lymph node metastasis, clinical TNM stage, alpha fetoprotein level, and tumor differentiation (P<0.05). Cox hazard model results showed that low expression of GAS5 mRNA was associated with poor prognosis (P<0.05).Conclusion GAS5 mRNA is expected to be a diagnostic and prognostic marker for patients with hepatocellular carcinoma.

Citation： QIU Jianguo, SHI Zhengrong, LI Ming. Expression of long non-coding RNA GAS5 in hepatocellular carcinoma tissues and its clinical significance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2019, 26(3): 301-306. doi: 10.7507/1007-9424.201807021 Copy

1.	Altekruse SF, McGlynn KA, Reichman ME. Hepatocellular carcinoma incidence, mortality, and survival trends in the United States from 1975 to 2005. J Clin Oncol, 2009, 27(9): 1485-1491.
2.	Chang CH, Chau GY, Lui WY, et al. Long-term results of hepatic resection for hepatocellular carcinoma originating from the noncirrhotic liver. Arch Surg, 2004, 139(3): 320-325.
3.	Gupta RA, Shah N, Wang KC, et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature, 2010, 464(7291): 1071-1076.
4.	Liu XH, Sun M, Nie FQ, et al. Lnc RNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer. Mol Cancer, 2014, 13: 92.
5.	Li L, Feng T, Lian Y, et al. Role of human noncoding RNAs in the control of tumorigenesis. Proc Natl Acad Sci U S A, 2009, 106(31): 12956-12961.
6.	Qiao HP, Gao WS, Huo JX, et al. Long non-coding RNA GAS5 functions as a tumor suppressor in renal cell carcinoma. Asian Pac J Cancer Prev, 2013, 14(2): 1077-1082.
7.	Zhang Z, Zhu Z, Watabe K, et al. Negative regulation of lncRNA GAS5 by miR-21. Cell Death Differ, 2013, 20(11): 1558-1568.
8.	Pickard MR, Williams GT. Regulation of apoptosis by long non-coding RNA GAS5 in breast cancer cells: implications for chemotherapy. Breast Cancer Res Treat, 2014, 145(2): 359-370.
9.	Pickard MR, Mourtada-Maarabouni M, Williams GT. Long non-coding RNA GAS5 regulates apoptosis in prostate cancer cell lines. Biochim Biophys Acta, 2013, 1832(10): 1613-1623.
10.	Shi X, Sun M, Liu H, et al. A critical role for the long non-coding RNA GAS5 in proliferation and apoptosis in non-small-cell lung cancer. Mol Carcinog, 2015, 54(Suppl 1): E1-E12.
11.	Cao Q, Wang N, Qi J, et al. Long non-coding RNA-GAS5 acts as a tumor suppressor in bladder transitional cell carcinoma via regulation of chemokine (C-C motif) ligand 1 expression. Mol Med Rep, 2016, 13(1): 27-34.
12.	Tu ZQ, Li RJ, Mei JZ, et al. Down-regulation of long non-coding RNA GAS5 is associated with the prognosis of hepatocellular carcinoma. Int J Clin Exp Pathol, 2014, 7(7): 4303-4309.
13.	景偉, 羅萍, 朱滿, 等. 血漿長鏈非編碼 RNA GAS5在肝細胞癌中表達水平及治療的評估. 中華檢驗醫學雜志, 2016, 39(8): 599-603.
14.	Edge SB, Byrd DR, Green FL, et al. American Joint Committee on Cancer Staging Manual. 7^th edition. New York: Springer, 2010: 242-251.
15.	Adesina AM, Rauch RA. Ganglioglioma and ganglio- cytoma//Adesina AM, Tihan T, Fuller CE, et al. Atlas of Pediatric Brian Tumors. Springer, 2010: 181-191.
16.	姚凱, 朱光輝, 單遠洲. 長鏈非編碼 RNA TUG1 和 UCA1 在結腸癌組織中的表達及臨床意義. 中國普通外科雜志, 2018, 27(4): 449-456.
17.	苗潤晨, 許鑫森, 陳偉, 等. 年齡因素對肝癌肝切除術患者長期預后的影響. 現代腫瘤醫學, 2014, 22(5): 1123-1126.
18.	高紀東, 邵永孚, 吳健雄, 等. 肝細胞癌肝切除術后預后影響因素分析. 中華肝膽外科雜志, 2005, 11(8): 515-517.
19.	蔣天安, 陳立斌, 陳芬, 等. 肝惡性腫瘤超聲引導下射頻消融短期有效性及影響因素分析. 中華超聲影像學雜志, 2013, 22(1): 25-29.
20.	石明, 張穎, 鐘崇, 等. 肝癌患者圍手術期外周血甲胎蛋白 mRNA 表達與術后復發的關系. 癌癥, 2008, 27(1): 83-87.
21.	呂文平, 董家鴻, 張文智, 等. 外科醫師手術操作對肝細胞癌預后的影響因素. 中國醫學科學院學報, 2008, 30(4): 386-392.
22.	梁冠林, 陳哲宇, 文天夫, 等. 常溫下連續阻斷半肝血流 60 min 在乙肝肝硬變患者肝切除術中的應用. 中國普外基礎與臨床雜志, 2008, 15(7): 503-507.
23.	劉景豐, 池閩輝, 曾金華, 等. 肝血流阻斷方法對肝切除術中大出血的影響. 中華外科雜志, 2010, 48(3): 177-180.
24.	Schones DE, Zhao K. Genome-wide approaches to studying chromatin modifications. Nat Rev Genet, 2008, 9(3): 179-191.
25.	孫甜甜, 房靜遠. 長非編碼 RNA 在肝癌中的研究進展. 腫瘤, 2011, 31(9): 867-870.
26.	Gabory A, Ripoche MA, Yoshimizu T, et al. The H19 gene: regulation and function of a non-coding RNA. Cytogenet Genome Res, 2006, 113(1-4): 188-193.
27.	Gutschner T, H?mmerle M, Eissmann M, et al. The noncoding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells. Cancer Res, 2013, 73(3): 1180-1189.
28.	Li H, An J, Wu M, et al. LncRNA HOTAIR promotes human liver cancer stem cell malignant growth through downregulation of SETD2. Oncotarget, 2015, 6(29): 27847-27864.
29.	Braconi C, Valeri N, Kogure T, et al. Expression and functional role of a transcribed noncoding RNA with an ultraconserved element in hepatocellular carcinoma. Proc Natl Acad Sci U S A, 2011, 108(2): 786-791.
30.	Braconi C, Kogure T, Valeri N, et al. microRNA-29 can regulate expression of the long non-coding RNA gene MEG3 in hepatocellular cancer. Oncogene, 2011, 30(47): 4750-4756.
31.	Lu X, Fang Y, Wang Z, et al. Downregulation of gas5 increases pancreatic cancer cell proliferation by regulating CDK6. Cell Tissue Res, 2013, 354(3): 891-896.

1. Altekruse SF, McGlynn KA, Reichman ME. Hepatocellular carcinoma incidence, mortality, and survival trends in the United States from 1975 to 2005. J Clin Oncol, 2009, 27(9): 1485-1491.
2. Chang CH, Chau GY, Lui WY, et al. Long-term results of hepatic resection for hepatocellular carcinoma originating from the noncirrhotic liver. Arch Surg, 2004, 139(3): 320-325.
3. Gupta RA, Shah N, Wang KC, et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature, 2010, 464(7291): 1071-1076.
4. Liu XH, Sun M, Nie FQ, et al. Lnc RNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer. Mol Cancer, 2014, 13: 92.
5. Li L, Feng T, Lian Y, et al. Role of human noncoding RNAs in the control of tumorigenesis. Proc Natl Acad Sci U S A, 2009, 106(31): 12956-12961.
6. Qiao HP, Gao WS, Huo JX, et al. Long non-coding RNA GAS5 functions as a tumor suppressor in renal cell carcinoma. Asian Pac J Cancer Prev, 2013, 14(2): 1077-1082.
7. Zhang Z, Zhu Z, Watabe K, et al. Negative regulation of lncRNA GAS5 by miR-21. Cell Death Differ, 2013, 20(11): 1558-1568.
8. Pickard MR, Williams GT. Regulation of apoptosis by long non-coding RNA GAS5 in breast cancer cells: implications for chemotherapy. Breast Cancer Res Treat, 2014, 145(2): 359-370.
9. Pickard MR, Mourtada-Maarabouni M, Williams GT. Long non-coding RNA GAS5 regulates apoptosis in prostate cancer cell lines. Biochim Biophys Acta, 2013, 1832(10): 1613-1623.
10. Shi X, Sun M, Liu H, et al. A critical role for the long non-coding RNA GAS5 in proliferation and apoptosis in non-small-cell lung cancer. Mol Carcinog, 2015, 54(Suppl 1): E1-E12.
11. Cao Q, Wang N, Qi J, et al. Long non-coding RNA-GAS5 acts as a tumor suppressor in bladder transitional cell carcinoma via regulation of chemokine (C-C motif) ligand 1 expression. Mol Med Rep, 2016, 13(1): 27-34.
12. Tu ZQ, Li RJ, Mei JZ, et al. Down-regulation of long non-coding RNA GAS5 is associated with the prognosis of hepatocellular carcinoma. Int J Clin Exp Pathol, 2014, 7(7): 4303-4309.
13. 景偉, 羅萍, 朱滿, 等. 血漿長鏈非編碼 RNA GAS5在肝細胞癌中表達水平及治療的評估. 中華檢驗醫學雜志, 2016, 39(8): 599-603.
14. Edge SB, Byrd DR, Green FL, et al. American Joint Committee on Cancer Staging Manual. 7^th edition. New York: Springer, 2010: 242-251.
15. Adesina AM, Rauch RA. Ganglioglioma and ganglio- cytoma//Adesina AM, Tihan T, Fuller CE, et al. Atlas of Pediatric Brian Tumors. Springer, 2010: 181-191.
16. 姚凱, 朱光輝, 單遠洲. 長鏈非編碼 RNA TUG1 和 UCA1 在結腸癌組織中的表達及臨床意義. 中國普通外科雜志, 2018, 27(4): 449-456.
17. 苗潤晨, 許鑫森, 陳偉, 等. 年齡因素對肝癌肝切除術患者長期預后的影響. 現代腫瘤醫學, 2014, 22(5): 1123-1126.
18. 高紀東, 邵永孚, 吳健雄, 等. 肝細胞癌肝切除術后預后影響因素分析. 中華肝膽外科雜志, 2005, 11(8): 515-517.
19. 蔣天安, 陳立斌, 陳芬, 等. 肝惡性腫瘤超聲引導下射頻消融短期有效性及影響因素分析. 中華超聲影像學雜志, 2013, 22(1): 25-29.
20. 石明, 張穎, 鐘崇, 等. 肝癌患者圍手術期外周血甲胎蛋白 mRNA 表達與術后復發的關系. 癌癥, 2008, 27(1): 83-87.
21. 呂文平, 董家鴻, 張文智, 等. 外科醫師手術操作對肝細胞癌預后的影響因素. 中國醫學科學院學報, 2008, 30(4): 386-392.
22. 梁冠林, 陳哲宇, 文天夫, 等. 常溫下連續阻斷半肝血流 60 min 在乙肝肝硬變患者肝切除術中的應用. 中國普外基礎與臨床雜志, 2008, 15(7): 503-507.
23. 劉景豐, 池閩輝, 曾金華, 等. 肝血流阻斷方法對肝切除術中大出血的影響. 中華外科雜志, 2010, 48(3): 177-180.
24. Schones DE, Zhao K. Genome-wide approaches to studying chromatin modifications. Nat Rev Genet, 2008, 9(3): 179-191.
25. 孫甜甜, 房靜遠. 長非編碼 RNA 在肝癌中的研究進展. 腫瘤, 2011, 31(9): 867-870.
26. Gabory A, Ripoche MA, Yoshimizu T, et al. The H19 gene: regulation and function of a non-coding RNA. Cytogenet Genome Res, 2006, 113(1-4): 188-193.
27. Gutschner T, H?mmerle M, Eissmann M, et al. The noncoding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells. Cancer Res, 2013, 73(3): 1180-1189.
28. Li H, An J, Wu M, et al. LncRNA HOTAIR promotes human liver cancer stem cell malignant growth through downregulation of SETD2. Oncotarget, 2015, 6(29): 27847-27864.
29. Braconi C, Valeri N, Kogure T, et al. Expression and functional role of a transcribed noncoding RNA with an ultraconserved element in hepatocellular carcinoma. Proc Natl Acad Sci U S A, 2011, 108(2): 786-791.
30. Braconi C, Kogure T, Valeri N, et al. microRNA-29 can regulate expression of the long non-coding RNA gene MEG3 in hepatocellular cancer. Oncogene, 2011, 30(47): 4750-4756.
31. Lu X, Fang Y, Wang Z, et al. Downregulation of gas5 increases pancreatic cancer cell proliferation by regulating CDK6. Cell Tissue Res, 2013, 354(3): 891-896.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of long non-coding RNA GAS5 in hepatocellular carcinoma tissues and its clinical significance

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