Expressions and clinical significance of Notch-2 protein and Numb protein in papillary thyroid carcinoma tissues_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YANG Fuqiang ^1,2 , WANG Yan ^1,2 , MA Jingyou ^1,2 , LI Zheng ¹ ,  LIU Zhen ^1,2

1. The Clinical College, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia Autonomous Rigon 028000, P. R. China;
2. Department of General Surgery, The Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia Autonomous Rigon 028000, P. R. China;

Corresponding?author：

LIU Zhen, Email: liuzhen95@sohu.com

Keywords：

papillary thyroid carcinoma; Notch-2 protein; Numb protein; immunohistochemistry

DOI：

10.7507/1007-9424.201707059

Video：

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Objective To investigate the expressions and clinical significance of Notch-2 protein and Numb protein in papillary thyroid carcinoma (PTC). Methods PTC tissues and its para-cancerous tissues of 50 patients with PTC who treated in The Affiliated Hospital of Inner Mongolia University for Nationalities from Mar. 2014 to Mar. 2017 were retrospectively collectied, to detect the expressions of Notch-2 protein and Numb protein by immunohistochenmical method. Results ① Expressions of Notch-2 protein and Numb protein in PTC tissues and para-cancerous tissues: the positive-expression rate of Notch-2 protein in PTC tissues was 82.00% (41/50), which was higher than that of para-cancerous tissues〔18.00% (9/50)〕, the difference was statistically significant (χ²=40.960, P<0.001). The positive-expression rate of Numb protein in PTC tissues was 66.00% (33/50), which was higher than that of para-cancerous tissues 〔0 (0/50) 〕, the difference was statistically significant (χ²=49.254, P<0.001). ② The relationship between expression of Notch-2 protein and expression of Numb protein in PTC tissues: there was a positive correlation between expressions of Notch-2 protein and Numb protein in PTC tissues (r_s=0.323, P=0.022). ③ The relationship between expressions of Notch-2 protein and Numb protein in PTC tissues and clinicopathological features of the PTC patients: the expression of Notch-2 protein in PTC tissues was not significantly correlated with gender, age, tumor diameter, capsule infiltration, cervical lymph node metastasis, and TNM staging (P>0.05). The expression of Numb protein in PTC tissues was not significantly correlated with gender, age, tumor diameter, and capsule infiltration (P>0.05), but was significantly correlated with cervical lymph node metastasis and TNM staging (P<0.05), the positive rates of Numb protein in patients of staging Ⅲ+Ⅳ group and cervical lymph node metastasis group were lower than those of patients in staging Ⅰ+Ⅱ group and non-cervical lymph node metastasis group. Conclusion The positive-expression rate of Notch-2 protein and Numb protein in PTC tissues are higher than those of para-cancerous tissues, and there is a positive correlation between them in PTC tissues, suggesting that there may be a synergistic effect in the course of PTC progression.

Citation： YANG Fuqiang, WANG Yan, MA Jingyou, LI Zheng, LIU Zhen. Expressions and clinical significance of Notch-2 protein and Numb protein in papillary thyroid carcinoma tissues. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2018, 25(1): 59-64. doi: 10.7507/1007-9424.201707059 Copy

1.	吳孟超, 吳在德. 黃家駟外科學. 北京: 人民衛生出版社, 2008: 1136-1139.
2.	陳孝平, 汪建平. 外科學. 第 8 版. 北京: 人民衛生出版社, 2013: 237-249.
3.	馬競優, 崔喆旭, 李華根, 等. Notch-1 在乳頭狀甲狀腺癌和頸部轉移淋巴結中的表達和臨床意義. 中國普外基礎與臨床雜志, 2017, 24(1): 70-73.
4.	曾令瑞, 韓玉貞, 馬英路, 等. 乳腺原發癌和相應淋巴結轉移癌干細胞 Wnt、Notch 信號通路相關分子的比較. 臨床與實驗病理學雜志, 2014, 30(9): 963-966.
5.	俞謙, 邵喜明, 李華順. NUMB 調控腫瘤增殖的機制研究. 四川大學學報 (醫學版), 2014, 45(5): 744-748.
6.	Guo LY, Li YM, Qiao L, et al. Notch2 regulates matrix metallopeptidase 9 via PI3K/AKT signaling in human gastric carcinoma cell MKN-45. World J Gastroenterol, 2012, 18(48): 7262-7270.
7.	Brown LM, Helmke SM, Hunsucker SW, et al. Quantitative and qualitative differences in protein expression between papillary thyroid carcinoma and normal thyroid tissue. Mol Carcinog, 2006, 45(8): 613-626.
8.	An H, Ge W, Xi Y, et al. Inscuteable maintains type I neuroblast lineage identity via Numb/Notch signaling in the Drosophila larval brain. J Genet Genomics, 2017, 44(3): 151-162.
9.	Zhang M, Qin Y, Zuo B, et al. Overexpression of NOTCH-regulated Ankyrin Repeat Protein is associated with papillary thyroid carcinoma progression. PLoS One, 2017, 12(2): e0167782.
10.	康艷. 甲狀腺乳頭狀癌組織中 Numb 與 p53 蛋白的表達及臨床意義. 長春: 吉林大學, 2013.
11.	曹子龍, 張卓, 胡建立, 等. NKD1 和 APC 在乳腺癌組織中的表達及意義. 中國普外基礎與臨床雜志, 2015, 22(10): 1226-1229.
12.	Yu J, Mai W, Cui Y, et al. Key genes and pathways predicted in papillary thyroid carcinoma based on bioinformatics analysis. J Endocrinol Invest, 2016, 39(11): 1285-1293.
13.	Jung CW, Kong JS, Seol H, et al.Expression of activated Notch1 and Hey1 in papillary thyroid carcinoma. Histopathology, 2017, 70(2): 301-308.
14.	Kim WB. A closer look at papillary thyroid carcinoma. Endocrinol Metab (Seoul), 2015, 30(1): 1-6.
15.	Rennstam K, McMichael N, Berglund P, et al. Numb protein expression correlates with a basal-like phenotype and cancer stem cell markers in primary breast cancer. Breast Cancer Res Treat, 2010, 122(2): 315-324.
16.	謝承志, 徐迅迪. Numb 的研究現狀及展望. 中國普通外科雜志, 2009, 18(9): 967-971.
17.	張奇, 宋鑫. Notch 信號通路在腫瘤干細胞中的研究進展. 中國腫瘤, 2014, 23(7): 585-590.
18.	鄧維葉, 陳艷峰, 郭朱明, 等. 甲狀腺乳頭狀癌 Notch-1 表達及復發相關因素分析. 腫瘤研究與臨床, 2015, 27(1): 11-14.
19.	楊晶金, 姚軍, 沈峰清, 等. Notch-1 信號通路與甲狀腺乳頭狀癌的關系. 中國細胞生物學學報, 2012, 34(2): 120-126.
20.	龔艷萍, 龔日祥, 楊敬, 等. 甲狀腺乳頭狀癌 BRAF 基因突變與臨床病理特征間的關系及臨床意義. 四川大學學報 (醫學版), 2013, 44(4): 662-665.
21.	Mseddi M, Ben Mansour R, Gouiia N, et al. A comparative study of nuclear 8-hydroxyguanosine expression in Autoimmune Thyroid Diseases and Papillary Thyroid Carcinoma and its relationship with p53, Bcl-2 and Ki-67 cancer related proteins. Adv Med Sci, 2017, 62(1): 45-51.
22.	C?rtes MCS, Rosario PW, Mour?o GF, et al. Influence of chronic lymphocytic thyroiditis on the risk of persistent and recurrent disease in patients with papillary thyroid carcinoma and elevated antithyroglobulin antibodies after initial therapy. Braz J Otorhinolaryngol, 2017, [Epub ahead of print].
23.	魏秋芳, 劉良裕, 劉楓, 等. Notch 信號通路與腫瘤干細胞及抑制劑研究進展. 中國藥學雜志, 2014, 49(6): 441-448.
24.	李佳, 孫夢黎, 黃健, 等. Notch 信號通路與生殖干細胞研究進展. 中國細胞生物學學報, 2014, 36(6): 841-846.
25.	萬健, 唐才喜, 馮斌, 等. 原發性肝癌中 Numb 和 VEGF 的表達及臨床意義. 中國普通外科雜志, 2015, 24(1): 39-44.
26.	萬伯順, 陳躍宇, 史佩東, 等. Numb 對結腸癌細胞遷移和侵襲的影響. 腫瘤, 2015, 35(8): 842-848.
27.	張平, 董文武, 崔俊帥, 等. 選擇性中央區淋巴結清掃治療 cN0 甲狀腺乳頭狀癌的臨床價值. 中國普外基礎與臨床雜志, 2014, 21(2): 182-186.
28.	孟曉敏, 溫樹信, 王斌全, 等. 甲狀腺乳頭狀癌原發灶外科治療的 Meta 分析. 臨床耳鼻咽喉頭頸外科雜志, 2015, 29(9): 835-840.

1. 吳孟超, 吳在德. 黃家駟外科學. 北京: 人民衛生出版社, 2008: 1136-1139.
2. 陳孝平, 汪建平. 外科學. 第 8 版. 北京: 人民衛生出版社, 2013: 237-249.
3. 馬競優, 崔喆旭, 李華根, 等. Notch-1 在乳頭狀甲狀腺癌和頸部轉移淋巴結中的表達和臨床意義. 中國普外基礎與臨床雜志, 2017, 24(1): 70-73.
4. 曾令瑞, 韓玉貞, 馬英路, 等. 乳腺原發癌和相應淋巴結轉移癌干細胞 Wnt、Notch 信號通路相關分子的比較. 臨床與實驗病理學雜志, 2014, 30(9): 963-966.
5. 俞謙, 邵喜明, 李華順. NUMB 調控腫瘤增殖的機制研究. 四川大學學報 (醫學版), 2014, 45(5): 744-748.
6. Guo LY, Li YM, Qiao L, et al. Notch2 regulates matrix metallopeptidase 9 via PI3K/AKT signaling in human gastric carcinoma cell MKN-45. World J Gastroenterol, 2012, 18(48): 7262-7270.
7. Brown LM, Helmke SM, Hunsucker SW, et al. Quantitative and qualitative differences in protein expression between papillary thyroid carcinoma and normal thyroid tissue. Mol Carcinog, 2006, 45(8): 613-626.
8. An H, Ge W, Xi Y, et al. Inscuteable maintains type I neuroblast lineage identity via Numb/Notch signaling in the Drosophila larval brain. J Genet Genomics, 2017, 44(3): 151-162.
9. Zhang M, Qin Y, Zuo B, et al. Overexpression of NOTCH-regulated Ankyrin Repeat Protein is associated with papillary thyroid carcinoma progression. PLoS One, 2017, 12(2): e0167782.
10. 康艷. 甲狀腺乳頭狀癌組織中 Numb 與 p53 蛋白的表達及臨床意義. 長春: 吉林大學, 2013.
11. 曹子龍, 張卓, 胡建立, 等. NKD1 和 APC 在乳腺癌組織中的表達及意義. 中國普外基礎與臨床雜志, 2015, 22(10): 1226-1229.
12. Yu J, Mai W, Cui Y, et al. Key genes and pathways predicted in papillary thyroid carcinoma based on bioinformatics analysis. J Endocrinol Invest, 2016, 39(11): 1285-1293.
13. Jung CW, Kong JS, Seol H, et al.Expression of activated Notch1 and Hey1 in papillary thyroid carcinoma. Histopathology, 2017, 70(2): 301-308.
14. Kim WB. A closer look at papillary thyroid carcinoma. Endocrinol Metab (Seoul), 2015, 30(1): 1-6.
15. Rennstam K, McMichael N, Berglund P, et al. Numb protein expression correlates with a basal-like phenotype and cancer stem cell markers in primary breast cancer. Breast Cancer Res Treat, 2010, 122(2): 315-324.
16. 謝承志, 徐迅迪. Numb 的研究現狀及展望. 中國普通外科雜志, 2009, 18(9): 967-971.
17. 張奇, 宋鑫. Notch 信號通路在腫瘤干細胞中的研究進展. 中國腫瘤, 2014, 23(7): 585-590.
18. 鄧維葉, 陳艷峰, 郭朱明, 等. 甲狀腺乳頭狀癌 Notch-1 表達及復發相關因素分析. 腫瘤研究與臨床, 2015, 27(1): 11-14.
19. 楊晶金, 姚軍, 沈峰清, 等. Notch-1 信號通路與甲狀腺乳頭狀癌的關系. 中國細胞生物學學報, 2012, 34(2): 120-126.
20. 龔艷萍, 龔日祥, 楊敬, 等. 甲狀腺乳頭狀癌 BRAF 基因突變與臨床病理特征間的關系及臨床意義. 四川大學學報 (醫學版), 2013, 44(4): 662-665.
21. Mseddi M, Ben Mansour R, Gouiia N, et al. A comparative study of nuclear 8-hydroxyguanosine expression in Autoimmune Thyroid Diseases and Papillary Thyroid Carcinoma and its relationship with p53, Bcl-2 and Ki-67 cancer related proteins. Adv Med Sci, 2017, 62(1): 45-51.
22. C?rtes MCS, Rosario PW, Mour?o GF, et al. Influence of chronic lymphocytic thyroiditis on the risk of persistent and recurrent disease in patients with papillary thyroid carcinoma and elevated antithyroglobulin antibodies after initial therapy. Braz J Otorhinolaryngol, 2017, [Epub ahead of print].
23. 魏秋芳, 劉良裕, 劉楓, 等. Notch 信號通路與腫瘤干細胞及抑制劑研究進展. 中國藥學雜志, 2014, 49(6): 441-448.
24. 李佳, 孫夢黎, 黃健, 等. Notch 信號通路與生殖干細胞研究進展. 中國細胞生物學學報, 2014, 36(6): 841-846.
25. 萬健, 唐才喜, 馮斌, 等. 原發性肝癌中 Numb 和 VEGF 的表達及臨床意義. 中國普通外科雜志, 2015, 24(1): 39-44.
26. 萬伯順, 陳躍宇, 史佩東, 等. Numb 對結腸癌細胞遷移和侵襲的影響. 腫瘤, 2015, 35(8): 842-848.
27. 張平, 董文武, 崔俊帥, 等. 選擇性中央區淋巴結清掃治療 cN0 甲狀腺乳頭狀癌的臨床價值. 中國普外基礎與臨床雜志, 2014, 21(2): 182-186.
28. 孟曉敏, 溫樹信, 王斌全, 等. 甲狀腺乳頭狀癌原發灶外科治療的 Meta 分析. 臨床耳鼻咽喉頭頸外科雜志, 2015, 29(9): 835-840.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expressions and clinical significance of Notch-2 protein and Numb protein in papillary thyroid carcinoma tissues

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