泡球蚴囊液對大鼠肝星狀細胞TGF-β1、IL-6及TNF-α表達的影響_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 李衍飛 ^1,2 , 邵軍 ³ , 王志鑫 ^1,2 , 姚寶洪 ³ , 陽丹才讓 ^1,2 , 任利 ^1,2 , 候立朝 ^1,2 , 王海久 ^1,2 , 周瀛 ^1,2 , 溫浩 ⁴ , 王虎 ⁵ ,  樊海寧 ^1,2

1. ;
2. ;
3. ;
4. ;
5. ;

Corresponding?author：

樊海寧, Email: fanhaining@medmail.com.cn

Keywords：

泡球蚴囊液; 肝星狀細胞; 轉化生長因子β1; 白細胞介素6; 腫瘤壞死因子-α

DOI：

10.7507/1007-9424.20160378

Video：

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目的觀察泡球蚴囊液對大鼠肝星狀細胞（HSC-T6）中轉化生長因子β1（TGF-β1）、白細胞介素6（IL-6）及腫瘤壞死因子-α（TNF-α）表達的影響，以初步揭示泡型肝包蟲病免疫調節作用的潛在機理。方法以泡球蚴囊液中蛋白成分的濃度代表囊液濃度，分別以0、0.01、0.025、0.05、0.1、0.2、0.4、0.9、1.7、3.4、6.8及13.5 mg/mL的泡球蚴囊液干預大鼠HSC-T6細胞，作用24 h后收集各組細胞上清，采用酶聯免疫吸附法檢測上清中TGF-β1、IL-6及TNF-α的濃度。結果以不同濃度泡球蚴囊液（0~13.5 mg/mL）干預后，HSC-T6細胞培養液上清中的TGF-β1濃度幾乎不變，各濃度組間兩兩比較差異均無統計學意義（P＞0.05）。當泡球蚴囊液濃度≤3.4 mg/mL時，上清中IL-6的濃度趨于平穩，各濃度組間兩兩比較差異均無統計學意義（P＞0.05）；當泡球蚴囊液濃度為6.8和13.5 mg/mL時，IL-6濃度較0 mg/mL組均升高，差異均有統計學意義（P＜0.01）。當泡球蚴囊液濃度≤0.2 mg/mL時，上清中的TNF-α濃度趨于平穩，各濃度組間兩兩比較差異均無統計學意義（P＞0.05）；當泡球蚴囊液濃度為0.4 mg/mL時，TNF-α濃度達到最大，為0 mg/mL組的3.53倍（P＜0.05）；此后隨濃度增高TNF-α濃度又逐漸降低，但仍高于0 mg/mL組（P＜0.05）。結論 IL-6和TNF-α可能在肝星狀細胞介導的泡型肝包蟲病的纖維化和組織損傷中扮演重要角色，而TGF-β1的作用尚不確定。

Citation： 李衍飛, 邵軍, 王志鑫, 姚寶洪, 陽丹才讓, 任利, 候立朝, 王海久, 周瀛, 溫浩, 王虎, 樊海寧. 泡球蚴囊液對大鼠肝星狀細胞TGF-β1、IL-6及TNF-α表達的影響. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(12): 1500-1502. doi: 10.7507/1007-9424.20160378 Copy

1.	趙慧, 白雪, 聶曉涵, 等.泡球蚴感染小鼠肝臟中IL-10和TGF-β1的動態變化.中國寄生蟲學與寄生蟲病雜志, 2012, 30(1):32-35.
2.	Parsons CJ, Takashima M, Rippe RA. Molecular mechanisms of hepatic fibrogenesis. J Gastroenterol Hepatol, 2007, 22 Suppl 1:S79-S84.
3.	Craig PS, Li T, Qiu J, et al. Echinococcosis and Tibetan communities. Emerg Infect Dis, 2008, 14(10):1674-1675.
4.	Yang YR, Craig PS, Sun T, et al. Echinococcosis in Ningxia Hui Autonomous Region, northwest China. Trans R Soc Trop Med Hyg, 2008, 102(4):319-328.
5.	張洋, 樊海寧.肝泡型包蟲病的治療進展.臨床外科雜志, 2014, 22(7):533-535.
6.	Polat P, Kantarci M, Alper F, et al. Hydatid disease from head to toe. Radiographics, 2003, 23(2):475-494.
7.	Eckert J, Deplazes P. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev, 2004, 17(1):107-135.
8.	溫浩, 徐明謙.實用包蟲病學.北京:科學出版社, 2007:15-19.
9.	王鴻, 李文桂.泡型包蟲病免疫發病機制研究進展.地方病通報, 2006, 21(1):98-100.
10.	謝文娟.泡球蚴感染小鼠肝臟基因表達譜的分析.新疆:新疆大學, 2010.
11.	Liu YH, Wang XG, Chen YT, et al. Computer tomography of liver in alveolar echinococcosis treated with albendazole. Trans R Soc Trop Med Hyg, 1993, 87(3):319-321.
12.	Friedman SL. Hepatic stellate cells:protean, multifunctional, and enigmatic cells of the liver. Physiol Rev, 2008, 88(1):125-172.
13.	Geerts A. On the origin of stellate cells:mesodermal, endodermal or neuro-ectodermal. J Hepatol, 2004, 40(2):331-334.
14.	劉昊, 粱廷波.肝星狀細胞在肝再生中的作用.中華消化外科雜志, 2011, 10(5):398-400.
15.	Brun P, Castagliuolo I, Pinzani M, et al. Exposure to bacterial cell wall products triggers an inflammatory phenotype in hepatic stellate cells. Am J Physiol Gastrointest Liver Physiol, 2005, 289(3):G571-G578.
16.	Thirunavukkarasu C, Watkins SC, Gandhi CR. Mechanisms of endotoxin-induced NO, IL-6, and TNF-alpha production in activated rat hepatic stellate cells:role of p38 MAPK. Hepatology, 2006, 44(2):389-398.
17.	Czaja MJ, Geerts A, Xu J, et al. Monocyte chemoattractant protein 1(MCP-1) expression occurs in toxic rat liver injury and human liver disease. J Leuk Biol, 1994, 55(1):120-126.
18.	王俊華, 盧曉梅, 張傳山, 等.轉化生長因子-β1及凋亡相關因子在泡球蚴感染宿主病灶旁免疫細胞中的表達及其意義.中華實驗外科雜志, 2011, 28(6):929-931.
19.	Hemeda H, Jakob M, Ludwig AK, et al. Interferon-gamma and tumor necrosis factor-alpha differentially affect cytokine expression and migration properties of mesenchymal stem cells. Stem Cells Dev, 2010, 19(5):693-706.
20.	Li HY, Ju D, Zhang DW, et al. Activation of TGF-β1-CD147 positive feedback loop in hepatic stellate cells promotes liver fibrosis. Sci Rep, 2015, 5:16552.
21.	Yu Y, Lu L, Qian X, et al. Antifibrotic effect of hepatocyte growth factor-expressing mesenchymal stem cells in small-for-size liver transplant rats. Stem Cells Dev, 2010, 19(6):903-914.
22.	Parekkadan B, van Poll D, Megeed Z, et al. Immunomodulation of activated hepatic stellate cells by mesenchymal stem cells. Biochem Biophys Res Commun, 2007, 363(2):247-252.
23.	Friedman SL. Hepatic fibrosis-overview. Toxicology, 2008, 254(3):120-129.
24.	李婧婷, 汪暉, 廖長秀, 等.肝星狀細胞激活的分子機制與抗肝纖維化治療.武漢大學學報:醫學版, 2007, 28(2):256-261.

1. 趙慧, 白雪, 聶曉涵, 等.泡球蚴感染小鼠肝臟中IL-10和TGF-β1的動態變化.中國寄生蟲學與寄生蟲病雜志, 2012, 30(1):32-35.
2. Parsons CJ, Takashima M, Rippe RA. Molecular mechanisms of hepatic fibrogenesis. J Gastroenterol Hepatol, 2007, 22 Suppl 1:S79-S84.
3. Craig PS, Li T, Qiu J, et al. Echinococcosis and Tibetan communities. Emerg Infect Dis, 2008, 14(10):1674-1675.
4. Yang YR, Craig PS, Sun T, et al. Echinococcosis in Ningxia Hui Autonomous Region, northwest China. Trans R Soc Trop Med Hyg, 2008, 102(4):319-328.
5. 張洋, 樊海寧.肝泡型包蟲病的治療進展.臨床外科雜志, 2014, 22(7):533-535.
6. Polat P, Kantarci M, Alper F, et al. Hydatid disease from head to toe. Radiographics, 2003, 23(2):475-494.
7. Eckert J, Deplazes P. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev, 2004, 17(1):107-135.
8. 溫浩, 徐明謙.實用包蟲病學.北京:科學出版社, 2007:15-19.
9. 王鴻, 李文桂.泡型包蟲病免疫發病機制研究進展.地方病通報, 2006, 21(1):98-100.
10. 謝文娟.泡球蚴感染小鼠肝臟基因表達譜的分析.新疆:新疆大學, 2010.
11. Liu YH, Wang XG, Chen YT, et al. Computer tomography of liver in alveolar echinococcosis treated with albendazole. Trans R Soc Trop Med Hyg, 1993, 87(3):319-321.
12. Friedman SL. Hepatic stellate cells:protean, multifunctional, and enigmatic cells of the liver. Physiol Rev, 2008, 88(1):125-172.
13. Geerts A. On the origin of stellate cells:mesodermal, endodermal or neuro-ectodermal. J Hepatol, 2004, 40(2):331-334.
14. 劉昊, 粱廷波.肝星狀細胞在肝再生中的作用.中華消化外科雜志, 2011, 10(5):398-400.
15. Brun P, Castagliuolo I, Pinzani M, et al. Exposure to bacterial cell wall products triggers an inflammatory phenotype in hepatic stellate cells. Am J Physiol Gastrointest Liver Physiol, 2005, 289(3):G571-G578.
16. Thirunavukkarasu C, Watkins SC, Gandhi CR. Mechanisms of endotoxin-induced NO, IL-6, and TNF-alpha production in activated rat hepatic stellate cells:role of p38 MAPK. Hepatology, 2006, 44(2):389-398.
17. Czaja MJ, Geerts A, Xu J, et al. Monocyte chemoattractant protein 1(MCP-1) expression occurs in toxic rat liver injury and human liver disease. J Leuk Biol, 1994, 55(1):120-126.
18. 王俊華, 盧曉梅, 張傳山, 等.轉化生長因子-β1及凋亡相關因子在泡球蚴感染宿主病灶旁免疫細胞中的表達及其意義.中華實驗外科雜志, 2011, 28(6):929-931.
19. Hemeda H, Jakob M, Ludwig AK, et al. Interferon-gamma and tumor necrosis factor-alpha differentially affect cytokine expression and migration properties of mesenchymal stem cells. Stem Cells Dev, 2010, 19(5):693-706.
20. Li HY, Ju D, Zhang DW, et al. Activation of TGF-β1-CD147 positive feedback loop in hepatic stellate cells promotes liver fibrosis. Sci Rep, 2015, 5:16552.
21. Yu Y, Lu L, Qian X, et al. Antifibrotic effect of hepatocyte growth factor-expressing mesenchymal stem cells in small-for-size liver transplant rats. Stem Cells Dev, 2010, 19(6):903-914.
22. Parekkadan B, van Poll D, Megeed Z, et al. Immunomodulation of activated hepatic stellate cells by mesenchymal stem cells. Biochem Biophys Res Commun, 2007, 363(2):247-252.
23. Friedman SL. Hepatic fibrosis-overview. Toxicology, 2008, 254(3):120-129.
24. 李婧婷, 汪暉, 廖長秀, 等.肝星狀細胞激活的分子機制與抗肝纖維化治療.武漢大學學報:醫學版, 2007, 28(2):256-261.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

泡球蚴囊液對大鼠肝星狀細胞TGF-β1、IL-6及TNF-α表達的影響

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