The Expressions and Significance of TypeⅠand Type Ⅲ Collagen Protein in Patients with Hepatic Alveolar Echinococcosis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 YANGDANCairang ^1,2 , SONGGe ^1,2 , ZHANGLing-qiang ^1,2 , YOUZhi-yuan ^1,2 , RENLi ^1,2 , HOULi-chao ^1,2 , WANGHai-jiu ^1,2 , ZHOUYing ^1,2 , WENHao ³ ,  FANHai-ning ^1,2

1. Department of Hepatopancreatobiliary Surgery, The Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China;
2. The Key Laboratory of Hydatid Disease Research in Qinghai Province, Xining 810001, Qinghai Province, China;
3. The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China;

Corresponding?author：

FANHai-ning, Email: fanhaining@medmail.com.cn

Keywords：

Hepatic alveolar echinococcosis; Echinococcus multilocularis; Collogen protein; Hepatic fibrosis

DOI：

10.7507/1007-9424.20160310

Video：

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Objective To investigate the expressions of type Ⅰ and type Ⅲ collagen protein in hepatic alveolar echinococcosis tissues, and to explore its relationship with the biological behavior in progress of hepatic alveolar echinococcosis (HAE). Methods Twenty samples of normal liver tissues and liver tissues at the edge of the lesion with HAE in our hospital from Jan. 2012 to Dec. 2014 were collected, and HE and Masson staining were performed. The pathological changes and the degree of fibrosis of liver tissues around HAE lesion were observed under light microscope. The expressions of type Ⅰ and type Ⅲ collagen protein in liver tissues were detected by immunohistochemical staining. Results The degree of liver fibrosis of liver tissues at the edge of the lesion with HAE was grade Ⅱ, and the degree of fibrosis of normal liver tissues was grade 0, the difference between the two was statistically significant (P < 0.05). The color index of type Ⅰand type Ⅲ collagen protein in the liver tissues at the edge of the lesion with HAE was 7.45±1.85 and 8.00±1.62, respectively, which were higher than those of normal liver tissues (3.10±1.02 and 3.50±0.89), the difference were statistically significant (t=-9.21, P=0.001;t=-10.88, P=0.001). Conclusions There is liver fibrosis around the lesion in the patients with HAE. HAE may promote the expressions of type Ⅰ and type Ⅲ collagen and then induce the occurrence of liver fibrosis.

Citation： YANGDAN Cairang, SONG Ge, ZHANG Ling-qiang, YOU Zhi-yuan, REN Li, HOU Li-chao, WANG Hai-jiu, ZHOU Ying, WEN Hao, FAN Hai-ning. The Expressions and Significance of TypeⅠand Type Ⅲ Collagen Protein in Patients with Hepatic Alveolar Echinococcosis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(10): 1209-1213. doi: 10.7507/1007-9424.20160310 Copy

1.	Vuitton DA, Zhou H, Bresson-Hadni S, et al. Epidemiology of alveolar echinococcosis with particular reference to China and Europe. Parasitology, 2003, 127 Suppl:87-107.
2.	路越晴, 鄧勇, 張靈強, 等. 泡型肝包蟲病的臨床治療進展. 中國普外基礎與臨床雜志, 2016, 23(5):547-550.
3.	侯玉虎, 馬淑梅, 樊海寧, 等. 青海達日、班瑪地區小學生肝包蟲病患病情況調查. 現代預防醫學, 2016, 43(13):2363-2365, 2381.
4.	張昀昊, 任利, 陽丹才讓, 等. 肝泡型包蟲病根治性切除163例回顧性分析. 中國普通外科雜志, 2016, 25(2):257-263.
5.	劉平, 李金花, 李印, 等. 包蟲病病原在我國的流行現狀及成因分析. 中國動物檢疫, 2016, 23(1):48-51.
6.	Lee UE, Friedman SL. Mechanisms of hepatic fibrogenesis. Best Pract Res Clin Gastroenterol, 2011, 25(2):195-206.
7.	吳向未, 陳雪玲, 彭心宇, 等. Ⅰ、Ⅲ、Ⅳ型膠原在肝包蟲囊腫周圍人體纖維囊壁中的特異性分層表達. 中國人獸共患病學報, 2004, 20(10):876-879.
8.	王俊華, 魏緒法, 張傳山, 等. Ⅰ、Ⅲ、Ⅳ膠原基因在泡球蚴感染中期小鼠肝臟中的表達及意義. 中國病原生物學雜志, 2012, 7(9):653-656.
9.	吳曉玲, 曾維政, 蔣明德, 等. 肝纖維化的信號轉導通路. 世界華人消化雜志, 2006, 14(22):2223-2228.
10.	楊艷宏, 蔡衛民, 金國梁, 等. 日本血吸蟲感染兔肝肌成纖維細胞的動態變化及其意義. 中華醫學雜志, 1999, 79(11):870-873.
11.	Mattern J, Stammler G, Koomagi R, et al. Association of vascular endothelial growth factor expression with tumor cell proliferation in ovarian carcinoma. Anticancer Res, 1997, 17(17):621-624.
12.	Chauchet A, Grenouillet F, Knapp J, et al. Increased incidence and characteristics of alveolar echinococcosis in patients with immunosuppression-associated conditions. Clin Infect Dis, 2014, 59(8):1095-1104.
13.	Wang J, Gottstein B. Immunoregulation in larval Echinococcus multilocularis, infection. Parasite Immunol, 2016, 38(3):182-192.
14.	Zhang F, Pang N, Zhu Y, et al. CCR7^lo PD-1^hi, CXCR5⁺, CD4⁺, T cells are positively correlated with levels of IL-21 in active and transitional cystic echinococcosis patients. BMC Infect Dis, 2015, 15(1):1-11.
15.	張夢然, 成軍. 肝纖維化研究進展. 國際消化病雜志, 2014,34(6):374-379.
16.	張斌. 肝纖維化的發病機理研究進展. 現代診斷與治療, 2004, 15(6):354-359.
17.	李偉道. 診斷肝纖維化、肝硬變的新進展:血清中細胞外基質成份的測定. 中國普外基礎與臨床雜志, 1997, 4(1):37-39.
18.	王要軍, 孫自勤. 肝竇內皮細胞與肝纖維化. 臨床肝膽病雜志, 1996, 12(3):115-117.
19.	Ryhanen L, Stenback F, Ala-Kokko L, et al. The effect of malotilate on type Ⅲ and type Ⅳ collagen, laminin and fibronectin metabolism in dimethylnitrosamine-induced liver fibrosis in the rat. J Hepatol, 1996, 24(2):238-245.
20.	Nakayama Y, Takahara T, Miyabayashi C, et al. Ultrastructural localization of type Ⅳcollagen and laminin in the Disse space of rat liver with carbon tetrachloride induced fibrosis. Liver, 1991, 11(5):260-271.
21.	Murawaki Y, Ikuta Y, Koda M, et al. Comparison of serum 7S fragment of type Ⅳ collagen and serum central triple-helix of type Ⅳ collagen for assessment of liver fibrosis in patients with chronic viral liver disease. J Hepatol, 1996, 24(2):148-154.
22.	Tsutsumi M, Takase S, Urashima S, et al. Serum markers for hepatic fibrosis in alcoholic liver disease:which is the best marker, type Ⅲ procollagen, type Ⅳ collagen, laminin, tissue inhibitor of metalloproteinase, or prolyl hydroxylase? Alcohol Clin Exp Res, 1996, 20(9):1512-1517.
23.	陳光文, 宋彬. 非侵入性方法評價肝纖維化. 中國普外基礎與臨床雜志, 2009, 16(5):414-417.
24.	Macwell PR, Flisiak R. Changes in serological biomarkers of liver funchtion and connective tissue turnover in chronic hepatitis B during lamivudine therapy. Biomarkerds, 2005, 10(6):475-484.
25.	Vuitton DA, Guerret-Stocker S, Carbillet JP, et al. Collagen immunotyping of the hepatic fibrosis in human alveolar echinococcosis. Z Parasitenkd, 1986, 72(1):97-104.
26.	Dar FK, Alkarmi T. 泡球蚴病的免疫病理學. 國際醫學寄生蟲病雜志, 1998, 25(6):270-272.

1. Vuitton DA, Zhou H, Bresson-Hadni S, et al. Epidemiology of alveolar echinococcosis with particular reference to China and Europe. Parasitology, 2003, 127 Suppl:87-107.
2. 路越晴, 鄧勇, 張靈強, 等. 泡型肝包蟲病的臨床治療進展. 中國普外基礎與臨床雜志, 2016, 23(5):547-550.
3. 侯玉虎, 馬淑梅, 樊海寧, 等. 青海達日、班瑪地區小學生肝包蟲病患病情況調查. 現代預防醫學, 2016, 43(13):2363-2365, 2381.
4. 張昀昊, 任利, 陽丹才讓, 等. 肝泡型包蟲病根治性切除163例回顧性分析. 中國普通外科雜志, 2016, 25(2):257-263.
5. 劉平, 李金花, 李印, 等. 包蟲病病原在我國的流行現狀及成因分析. 中國動物檢疫, 2016, 23(1):48-51.
6. Lee UE, Friedman SL. Mechanisms of hepatic fibrogenesis. Best Pract Res Clin Gastroenterol, 2011, 25(2):195-206.
7. 吳向未, 陳雪玲, 彭心宇, 等. Ⅰ、Ⅲ、Ⅳ型膠原在肝包蟲囊腫周圍人體纖維囊壁中的特異性分層表達. 中國人獸共患病學報, 2004, 20(10):876-879.
8. 王俊華, 魏緒法, 張傳山, 等. Ⅰ、Ⅲ、Ⅳ膠原基因在泡球蚴感染中期小鼠肝臟中的表達及意義. 中國病原生物學雜志, 2012, 7(9):653-656.
9. 吳曉玲, 曾維政, 蔣明德, 等. 肝纖維化的信號轉導通路. 世界華人消化雜志, 2006, 14(22):2223-2228.
10. 楊艷宏, 蔡衛民, 金國梁, 等. 日本血吸蟲感染兔肝肌成纖維細胞的動態變化及其意義. 中華醫學雜志, 1999, 79(11):870-873.
11. Mattern J, Stammler G, Koomagi R, et al. Association of vascular endothelial growth factor expression with tumor cell proliferation in ovarian carcinoma. Anticancer Res, 1997, 17(17):621-624.
12. Chauchet A, Grenouillet F, Knapp J, et al. Increased incidence and characteristics of alveolar echinococcosis in patients with immunosuppression-associated conditions. Clin Infect Dis, 2014, 59(8):1095-1104.
13. Wang J, Gottstein B. Immunoregulation in larval Echinococcus multilocularis, infection. Parasite Immunol, 2016, 38(3):182-192.
14. Zhang F, Pang N, Zhu Y, et al. CCR7^lo PD-1^hi, CXCR5⁺, CD4⁺, T cells are positively correlated with levels of IL-21 in active and transitional cystic echinococcosis patients. BMC Infect Dis, 2015, 15(1):1-11.
15. 張夢然, 成軍. 肝纖維化研究進展. 國際消化病雜志, 2014,34(6):374-379.
16. 張斌. 肝纖維化的發病機理研究進展. 現代診斷與治療, 2004, 15(6):354-359.
17. 李偉道. 診斷肝纖維化、肝硬變的新進展:血清中細胞外基質成份的測定. 中國普外基礎與臨床雜志, 1997, 4(1):37-39.
18. 王要軍, 孫自勤. 肝竇內皮細胞與肝纖維化. 臨床肝膽病雜志, 1996, 12(3):115-117.
19. Ryhanen L, Stenback F, Ala-Kokko L, et al. The effect of malotilate on type Ⅲ and type Ⅳ collagen, laminin and fibronectin metabolism in dimethylnitrosamine-induced liver fibrosis in the rat. J Hepatol, 1996, 24(2):238-245.
20. Nakayama Y, Takahara T, Miyabayashi C, et al. Ultrastructural localization of type Ⅳcollagen and laminin in the Disse space of rat liver with carbon tetrachloride induced fibrosis. Liver, 1991, 11(5):260-271.
21. Murawaki Y, Ikuta Y, Koda M, et al. Comparison of serum 7S fragment of type Ⅳ collagen and serum central triple-helix of type Ⅳ collagen for assessment of liver fibrosis in patients with chronic viral liver disease. J Hepatol, 1996, 24(2):148-154.
22. Tsutsumi M, Takase S, Urashima S, et al. Serum markers for hepatic fibrosis in alcoholic liver disease:which is the best marker, type Ⅲ procollagen, type Ⅳ collagen, laminin, tissue inhibitor of metalloproteinase, or prolyl hydroxylase? Alcohol Clin Exp Res, 1996, 20(9):1512-1517.
23. 陳光文, 宋彬. 非侵入性方法評價肝纖維化. 中國普外基礎與臨床雜志, 2009, 16(5):414-417.
24. Macwell PR, Flisiak R. Changes in serological biomarkers of liver funchtion and connective tissue turnover in chronic hepatitis B during lamivudine therapy. Biomarkerds, 2005, 10(6):475-484.
25. Vuitton DA, Guerret-Stocker S, Carbillet JP, et al. Collagen immunotyping of the hepatic fibrosis in human alveolar echinococcosis. Z Parasitenkd, 1986, 72(1):97-104.
26. Dar FK, Alkarmi T. 泡球蚴病的免疫病理學. 國際醫學寄生蟲病雜志, 1998, 25(6):270-272.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The Expressions and Significance of TypeⅠand Type Ⅲ Collagen Protein in Patients with Hepatic Alveolar Echinococcosis

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