Experimental Study of Rat Bone Marrow Endothelial Progenitor Cells Labeled with SPIO in Vitro_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 LIAOChuan-jun ,  ZHANGWang-de

Department of Vascular Surgery, Beijing Chaoyang Hospital, The Affiliated Hospital of Capital Medical University, Beijing 100020, China;

Corresponding?author：

ZHANGWang-de, Email: drwangde@vip.sina.com

Keywords：

Endothelial progenitor cell; Superparamagnetic iron oxide; Labeling

DOI：

10.7507/1007-9424.20160209

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To explore optimal conditions of isolation, culture and labeled with superparamagnetic iron oxide (SPIO) in vitro of rat bone marrow endothelial progenitor cells, and lay the foundations for the further EPCs tracer study in vivo. Methods The EPCs derived from rat bone marrow were isolated and cultured by using density gradient centrifugation, which were labeled with different concentrations SPIO, Prussian blue staining was used to detect the cells labeling rate, MTT assay was used to detect the cells proliferation activity, and Trypan blue staining was used to detect the cells vitality. Results EPCs gradually growed in monolayer arrangement about 7 d after cultured. When the concentration of SPIO was 50μg/mL, the highest labeling rate of Prussian blue staining was 90%, the growth state of labeled EPCs were good, and could normal adherent growth and passage. At this time, the cell viability and proliferation activity were the highest through trypan blue staining and MTT assay. Conclusions EPCs can be labeled with SPIO easily and efficiently when the concentration was 50μg/mL?without interference on the viability and proliferation activity, which lay the foundations for the further EPCs tracer study in vivo.

Citation： LIAOChuan-jun, ZHANGWang-de. Experimental Study of Rat Bone Marrow Endothelial Progenitor Cells Labeled with SPIO in Vitro. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(7): 794-797. doi: 10.7507/1007-9424.20160209 Copy

1.	Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science, 1997, 14(275): 964-967.
2.	Blum A. Endoethelial progenitor cells are affected by medications and estrogen. Isr Med Assoc J, 2015, 17(9): 578-580.
3.	Xu JY, Lee YK, Wang Y, et al. Therapeutic application of endothelial progenitor cells for treatment of cardiovascular diseases. Curr Stem Cell Res Ther, 2014, 9(5): 401-414.
4.	Hill JM, Dick AJ, Raman VK, et al. Serial cardiac magnetic resonance imaging of injected mesenchymal stem cells. Circulation, 2003, 108(8): 1009-1014.
5.	Jülke H, Veit C, Ribitsch I, et al. Comparative labelling of equine and ovine multipotent stromal cells with superparamagnetic iron oxide particles for magnetic resonance imaging in vitro. Cell Transplant, 2013, 40(3): 288-295.
6.	Sun JH, Zhang YL, Nie CH, et al. in vitro labeling of endothelial progenitor cells isolated from peripheral blood with superparama-gnetic iron oxidenanoparticles. Mol Med Rep, 2012, 6(2): 282-286.
7.	Zhou B, Li D, Qian J, et al. MR tracking of SPIO-labeled mesench-ymal stem cells in rats with liver fibrosis could not monitor the cells accurately. Contrast Media Mol Imaging, 2015, 10(6): 473-480.
8.	Duan L, Yang F, Song L, et al. Controlled assembly of magnetic nanoparticles on microbubbles for multimodal imaging. Soft Matter, 2015, 11(27): 5492-5500.
9.	廖傳軍, 郭連瑞, 楊寶鐘, 等.重組人pEGFP-heNOS質粒轉染人內皮祖細胞及其條件優化.中國微循環, 2009, 13(1): 11-14.
10.	Bruce IJ, Sen T. Surface modification of magnetic nanoparticles with alkoxysilanes and their application in magnetic bioseparations. Langmuir, 2005, 21(15):7029-7035.
11.	Park YC, Paulsen J, Nap RJ, et al. Adsorption of superparamagnetic iron oxide nanoparticles on silica and calcium carbonate sand. Langmuir, 2014, 30(3): 784-792.
12.	康濤, 李曉強, 孟慶友, 等.新型超順磁性氧化鐵標記血管內皮祖細胞的研究.中國血管外科雜志, 2012, 4(2): 108-111.
13.	崔碧, 李健丁, 張瑞平.大鼠骨髓內皮祖細胞的SPIO標記及檢測.中國醫藥導報, 2011, 8(9): 33-35, 63.
14.	Shah QA, Tan X, Bi S, et al. Differential characteristics and in vitro angiogenesis of bone marrow-and peripheral blood-derivedendo-thelial progenitor cells: evidence from avian species. Cell Prolif, 2014, 47(4): 290-298.
15.	Chevalier F, Lavergne M, Negroni E, et al. Glycosaminoglycan mimetic improves enrichment and cell functions of human endothe-lial progenitor cell colonies. Stem Cell Res, 2014, 12(3): 703-715.
16.	Cheng SM, Chang SJ, Tsai TN, et al. Differential expression of distinct surface markers in early endothelial progenitor cells and monocyte-derived macrophages. Gene Expr, 2013, 16(1): 15-24.
17.	Barthelmes D, Irhimeh MR, Gillies MC, et al. Isolation and charac-terization of mouse bone marrow-derived Lin-/VEGF-R2+ progen-itor cells. Ann Hematol, 2013, 92(11): 1461-1472.
18.	廖傳軍, 郭秒, 郭連瑞, 等.重組人pEGFP-heNOS質粒的構建及其在人內皮祖細胞中的表達.中華實驗外科雜志, 2006, 23(11): 1364-1366.
19.	Hill JM, Dick AJ, Raman VK, et al. Serial cardiac magnetic resonance imaging of injected mesenchymal stem cells. Circulation, 2003, 108 (8): 1009-1014.
20.	Uta D, Walter B, Marco M, et al. In vivo tracking and fate of intra-articularly injected superparamagnetic iron oxide particle-labeled multipotent stromal cells in an ovine model of osteoarthritis. Cell Transplant, 2015, 24(11): 2379-2390.
21.	Wei MQ, Wen DD, Wang XY, et al. Experimental study of endothe-lial progenitor cells labeled with superparamagnetic iron oxide in vitro. Mol Med Rep, 2015, 11(5):3814-3819.
22.	Heyn C, Bowen, CV, Rutt BK, et al. Detection threshold of single SPIO-labeled cells with FIESTA. Magn Reson Med, 2005, 53(2): 312-320.
23.	Jacobs RE, Fraser SE. Magnetic resonance microscopy of embryonic cell lineages and movements. Science, 1994, 263(5147): 681-684.
24.	Modo M, Mellodew K, Cash D, et al. Mapping transplanted stem cell migration after stroke: a serial In vivo magnetic resonance imaging study. Neuroimage, 2004, 21(3): 311-317.
25.	鄭敏文, 宦怡, 徐鍵, 等.超順磁性氧化鐵標記骨髓間充質干細胞的磁共振成像研究.中國醫學影像技術, 2006, 22(8): 1129-1134.
26.	Zhang GJ, Safran M, Wei W, et al. Bioluminescent imaging of Cdk2 inhibition In vivo. Nat Med, 2004, 10(6): 643-648.

1. Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science, 1997, 14(275): 964-967.
2. Blum A. Endoethelial progenitor cells are affected by medications and estrogen. Isr Med Assoc J, 2015, 17(9): 578-580.
3. Xu JY, Lee YK, Wang Y, et al. Therapeutic application of endothelial progenitor cells for treatment of cardiovascular diseases. Curr Stem Cell Res Ther, 2014, 9(5): 401-414.
4. Hill JM, Dick AJ, Raman VK, et al. Serial cardiac magnetic resonance imaging of injected mesenchymal stem cells. Circulation, 2003, 108(8): 1009-1014.
5. Jülke H, Veit C, Ribitsch I, et al. Comparative labelling of equine and ovine multipotent stromal cells with superparamagnetic iron oxide particles for magnetic resonance imaging in vitro. Cell Transplant, 2013, 40(3): 288-295.
6. Sun JH, Zhang YL, Nie CH, et al. in vitro labeling of endothelial progenitor cells isolated from peripheral blood with superparama-gnetic iron oxidenanoparticles. Mol Med Rep, 2012, 6(2): 282-286.
7. Zhou B, Li D, Qian J, et al. MR tracking of SPIO-labeled mesench-ymal stem cells in rats with liver fibrosis could not monitor the cells accurately. Contrast Media Mol Imaging, 2015, 10(6): 473-480.
8. Duan L, Yang F, Song L, et al. Controlled assembly of magnetic nanoparticles on microbubbles for multimodal imaging. Soft Matter, 2015, 11(27): 5492-5500.
9. 廖傳軍, 郭連瑞, 楊寶鐘, 等.重組人pEGFP-heNOS質粒轉染人內皮祖細胞及其條件優化.中國微循環, 2009, 13(1): 11-14.
10. Bruce IJ, Sen T. Surface modification of magnetic nanoparticles with alkoxysilanes and their application in magnetic bioseparations. Langmuir, 2005, 21(15):7029-7035.
11. Park YC, Paulsen J, Nap RJ, et al. Adsorption of superparamagnetic iron oxide nanoparticles on silica and calcium carbonate sand. Langmuir, 2014, 30(3): 784-792.
12. 康濤, 李曉強, 孟慶友, 等.新型超順磁性氧化鐵標記血管內皮祖細胞的研究.中國血管外科雜志, 2012, 4(2): 108-111.
13. 崔碧, 李健丁, 張瑞平.大鼠骨髓內皮祖細胞的SPIO標記及檢測.中國醫藥導報, 2011, 8(9): 33-35, 63.
14. Shah QA, Tan X, Bi S, et al. Differential characteristics and in vitro angiogenesis of bone marrow-and peripheral blood-derivedendo-thelial progenitor cells: evidence from avian species. Cell Prolif, 2014, 47(4): 290-298.
15. Chevalier F, Lavergne M, Negroni E, et al. Glycosaminoglycan mimetic improves enrichment and cell functions of human endothe-lial progenitor cell colonies. Stem Cell Res, 2014, 12(3): 703-715.
16. Cheng SM, Chang SJ, Tsai TN, et al. Differential expression of distinct surface markers in early endothelial progenitor cells and monocyte-derived macrophages. Gene Expr, 2013, 16(1): 15-24.
17. Barthelmes D, Irhimeh MR, Gillies MC, et al. Isolation and charac-terization of mouse bone marrow-derived Lin-/VEGF-R2+ progen-itor cells. Ann Hematol, 2013, 92(11): 1461-1472.
18. 廖傳軍, 郭秒, 郭連瑞, 等.重組人pEGFP-heNOS質粒的構建及其在人內皮祖細胞中的表達.中華實驗外科雜志, 2006, 23(11): 1364-1366.
19. Hill JM, Dick AJ, Raman VK, et al. Serial cardiac magnetic resonance imaging of injected mesenchymal stem cells. Circulation, 2003, 108 (8): 1009-1014.
20. Uta D, Walter B, Marco M, et al. In vivo tracking and fate of intra-articularly injected superparamagnetic iron oxide particle-labeled multipotent stromal cells in an ovine model of osteoarthritis. Cell Transplant, 2015, 24(11): 2379-2390.
21. Wei MQ, Wen DD, Wang XY, et al. Experimental study of endothe-lial progenitor cells labeled with superparamagnetic iron oxide in vitro. Mol Med Rep, 2015, 11(5):3814-3819.
22. Heyn C, Bowen, CV, Rutt BK, et al. Detection threshold of single SPIO-labeled cells with FIESTA. Magn Reson Med, 2005, 53(2): 312-320.
23. Jacobs RE, Fraser SE. Magnetic resonance microscopy of embryonic cell lineages and movements. Science, 1994, 263(5147): 681-684.
24. Modo M, Mellodew K, Cash D, et al. Mapping transplanted stem cell migration after stroke: a serial In vivo magnetic resonance imaging study. Neuroimage, 2004, 21(3): 311-317.
25. 鄭敏文, 宦怡, 徐鍵, 等.超順磁性氧化鐵標記骨髓間充質干細胞的磁共振成像研究.中國醫學影像技術, 2006, 22(8): 1129-1134.
26. Zhang GJ, Safran M, Wei W, et al. Bioluminescent imaging of Cdk2 inhibition In vivo. Nat Med, 2004, 10(6): 643-648.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Study of Rat Bone Marrow Endothelial Progenitor Cells Labeled with SPIO in Vitro

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content