Expression of Alpha B-Crystallin in Colorectal Cancer and Its Clinical Significances_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 ZHUJun ¹ , FENGYing ² , ZHANGFeng ³ , YUANDe-feng ⁴ , ZHOUQi ¹ , JINQiang ¹ ,  ZHAODa ³

1. Department of Pathology, Donggang Branch of The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China;
2. Department of Pathology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China;
3. Department of Medical Oncology, Donggang Branch of The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China;
4. Department of General Surgery, Donggang Branch of The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China;

Corresponding?author：

ZHAODa, Email: zhaodamail@163.com

Keywords：

Alpha B-crystallin; Colorectal cancer; Immunohistochemistry method

DOI：

10.7507/1007-9424.20150348

Video：

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Objective To explore the expression of Cryab in human colorectal cancer tissue and its clinical significances. Methods Ten pairs of colorectal cancer specimens of early recurrence and late recurrence in the First Hospital of Lanzhou University were collected, the Cryab mRNA expression was detected by RT-PCR. The colorectal cancer tissues and corresponding adjacent tissues of 100 patients underwent radical resection from January 2005 to December 2008 in the Donggang Branch of The First Hospital and The First Hospital of Lanzhou University were collected, the expression of Cryab protein in the colorectal cancer tissues and corresponding adjacent tissues of 100 patients with colorectal cancer were detected by immunohistochemistry SP technique. The relation between the expression of Cryab protein and the age, gender, number of tumors, tumor size, TNM stage, degree of differentiation, lymph node metastasis, or depth of invasion of the patients was analyzed. The influence of Cryab expression on prognosis of patients with colorectal cancer was evaluated by univariate and multivariate analysis. Results ①The expression of Cryab mRNA in the colorectal cancer tissues with early recurrence was significantly higher than that in the colorectal cancer tissues with late recurrence (P < 0.05).②The expression of Cryab protein in the colorectal cancer tissues was significantly higher than that in the corresponding adjacent tissues (P < 0.05).③The over-expression of Cryab protein in the colorectal cancer tissues was correlated with the tumor size, degree of differentiation, lymph node metastasis, or TNM stages (P < 0.05), but not correlated with the age, sex, and number of tumors (P > 0.05).④The lymph node metastasis and expression of Cryab protein were the independent risk factors for the prognosis of patients with colorectal cancer.⑤The overall survival and disease free survival of patients with high-expression of Cryab protein were significantly lower than those in the patients with lower-expression of Cryab protein. Conclusions The over-expression of Cryab is closely related to the invasion and progression of colorectal cancer, they might be together involved in the progression of colorectal cancer. Cryab might be a good marker for monitoring the malignant degree and the prognosis of colorectal cancer.

Citation： ZHUJun, FENGYing, ZHANGFeng, YUANDe-feng, ZHOUQi, JINQiang, ZHAODa. Expression of Alpha B-Crystallin in Colorectal Cancer and Its Clinical Significances. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(11): 1332-1337. doi: 10.7507/1007-9424.20150348 Copy

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2.	張利忠, 周建平.結直腸癌的新輔助化療.長治醫學院學報, 2014, 28(2):157-160.
3.	晏仲舒.大腸癌輔助化療.中國普通外科雜志, 2002, 11(11):641-642.
4.	Dudeja V, Vickers SM, Saluja AK. The role of heat shock proteins in gastrointestinal diseases. Gut, 2009, 58(7):1000-1009.
5.	Arrigo AP. sHsp as novel regulators of programmed cell death and tumorigenicity. Pathol Biol (Paris), 2000, 48(3):280-288.
6.	Qin H, Ni Y, Tong J, et al. Elevated expression of CRYAB predicts unfavorable prognosis in non-small cell lung cancer. Med Oncol, 2014, 31(8):142.
7.	Van de Schootbrugge C, Schults EM, Bussink J, et al. Effect of hypoxia on the expression ofαB-crystallin in head and neck squamous cell carcinoma. BMC Cancer, 2014, 14:252.
8.	Chen ZJ, Ruan Q, Han S, et al. Discovery of structure-based small molecular inhibitor of alpha B-crystallin against basal-like/triplenegative breast cancer development in vitro and in vivo. Breast Cancer Res Treat, 2014, 145(1):45-59.
9.	Poon RT, Fan ST, Ng IO, et al. Diferent risk factors and prognosis for early and late intrahepatic recurence after resection. Cancer, 2000, 89(3):500-507.
10.	李淵, 馬家馳, 李一平, 等. PTEN siRNA對結腸癌細胞增殖和侵襲的影響.中國普外基礎與臨床雜志, 2015, 22(5):544-548.
11.	Volkmann J, Reuning U, Rudelius M, et al. High expression of crystallinαB represents an independent molecular marker for unfavourable ovarian cancer patient outcome and impairs TRAILand cisplatin-induced apoptosis in human ovarian cancer cells. Int J Cancer, 2013, 132(12):2820-2832.
12.	Folio C1, Mora MI, Zalacain M, et al. Proteomic analysis of chemonaive pediatric osteosarcomas and corresponding normal bone reveals multiple altered molecular targets. J Proteome Res, 2009, 8(8):3882-3888.
13.	Zuo RJ, Zhao YC, Lei W, et al. CrystallinαB acts as a molecular guard in mouse decidualization:regulation and function during early pregnancy. FEBS Lett, 2014, 588(17):2944-2951.
14.	高石磊.熱休克蛋白90在急性髓系白血病患者血清中的表達及臨床意義.石家莊:河北醫科大學, 2014.
15.	Moyano JV, Evans JR, Chen F, et al. AlphaB-crystallin is a novel oncoprotein that predicts poor clinical outcome in breast cancer. J Clin Invest, 2006, 116(1):261-270.
16.	賴清波. miR-96和TACSTD1在原發性肝細胞癌根治術后早期復發患者中的表達及臨床意義.廣州:南方醫科大學, 2014.
17.	Gruvberger-Saal SK, Parsons R. Is the small heat shock protein alpha B-crystallin an oncogene? J Clin Invest, 2006, 116(1):30-32.
18.	Parcellier A, Schmitt E, Brunet M, et al. Small heat shock proteins HSP27 and alphaB-crystallin:cytoprotective and oncogenic functions. Antioxid Redox Signal, 2005, 7:404-413.
19.	Mao YW, Xiang H, Wang J, et al. Human bcl-2 gene attenuates the ability of rabbit lens epithelial cells against H2O2-induced apoptosis through down-regulation of the alpha B-crystallin gene. J Biol Chem, 2001, 276(46):43435-43445.
20.	Mao YW, Liu JP, Xiang H, et al. Human alpha A-and alpha B-crystallins bind to Bax and Bcl-X(S) to sequester their translocation during staurosporine-induced apoptosis. Cell Death Differ, 2004, 11(5):512-526.
21.	Filomeni G, Aquilano K, Rotilio G, et al. Antiapoptotic response to induced GSH depletion:involvement of heat shock proteins and NF-kappaB activation. Antioxid Redox Signal, 2005, 7(3/4):446-455.
22.	Lamoureux F, Thomas C, Yin MJ, et al. Suppression of heat shock protein 27 using OGX-427 induces endoplasmic reticulum stress and potentiates heat shock protein 90 inhibitors to delay castrateresistant prostate cancer. Eur Urol, 2014, 66(1):145-155.
23.	黃曉勇.小熱休克蛋白alpha B-Crystallin (Cryab)在肝癌侵襲轉移中的分子機制及與索拉非尼耐藥研究.上海:復旦大學, 2013.
24.	周娟.人食管癌細胞熱休克蛋白70肽復合物的提取及其抗瘤活性的研究.鄭州:鄭州大學, 2006.
25.	Aoyama A, Steiger RH, Frohli E, et al. Expression of alpha B-crystallin in human brain tumors. Int J Cancer, 1993, 55:760-764.
26.	Chin D, Boyle GM, Williams RM, et al. Alpha B-crystallin, a new independent marker for poor prognosis in head and neck cancer. Laryngoscope, 2005, 115:1239-1242.
27.	唐奇, 馮振卿. CRYAB基因在HCC中的表達、臨床預后分析及抗凋亡功能研究.南京:南京醫科大學, 2007.

1. ?Torre LA, Bray F, Siegel RL, et al. Global cancer statistics, 2012. CA Cancer J Clin, 2012, 65(2):87-108.
2. 張利忠, 周建平.結直腸癌的新輔助化療.長治醫學院學報, 2014, 28(2):157-160.
3. 晏仲舒.大腸癌輔助化療.中國普通外科雜志, 2002, 11(11):641-642.
4. Dudeja V, Vickers SM, Saluja AK. The role of heat shock proteins in gastrointestinal diseases. Gut, 2009, 58(7):1000-1009.
5. Arrigo AP. sHsp as novel regulators of programmed cell death and tumorigenicity. Pathol Biol (Paris), 2000, 48(3):280-288.
6. Qin H, Ni Y, Tong J, et al. Elevated expression of CRYAB predicts unfavorable prognosis in non-small cell lung cancer. Med Oncol, 2014, 31(8):142.
7. Van de Schootbrugge C, Schults EM, Bussink J, et al. Effect of hypoxia on the expression ofαB-crystallin in head and neck squamous cell carcinoma. BMC Cancer, 2014, 14:252.
8. Chen ZJ, Ruan Q, Han S, et al. Discovery of structure-based small molecular inhibitor of alpha B-crystallin against basal-like/triplenegative breast cancer development in vitro and in vivo. Breast Cancer Res Treat, 2014, 145(1):45-59.
9. Poon RT, Fan ST, Ng IO, et al. Diferent risk factors and prognosis for early and late intrahepatic recurence after resection. Cancer, 2000, 89(3):500-507.
10. 李淵, 馬家馳, 李一平, 等. PTEN siRNA對結腸癌細胞增殖和侵襲的影響.中國普外基礎與臨床雜志, 2015, 22(5):544-548.
11. Volkmann J, Reuning U, Rudelius M, et al. High expression of crystallinαB represents an independent molecular marker for unfavourable ovarian cancer patient outcome and impairs TRAILand cisplatin-induced apoptosis in human ovarian cancer cells. Int J Cancer, 2013, 132(12):2820-2832.
12. Folio C1, Mora MI, Zalacain M, et al. Proteomic analysis of chemonaive pediatric osteosarcomas and corresponding normal bone reveals multiple altered molecular targets. J Proteome Res, 2009, 8(8):3882-3888.
13. Zuo RJ, Zhao YC, Lei W, et al. CrystallinαB acts as a molecular guard in mouse decidualization:regulation and function during early pregnancy. FEBS Lett, 2014, 588(17):2944-2951.
14. 高石磊.熱休克蛋白90在急性髓系白血病患者血清中的表達及臨床意義.石家莊:河北醫科大學, 2014.
15. Moyano JV, Evans JR, Chen F, et al. AlphaB-crystallin is a novel oncoprotein that predicts poor clinical outcome in breast cancer. J Clin Invest, 2006, 116(1):261-270.
16. 賴清波. miR-96和TACSTD1在原發性肝細胞癌根治術后早期復發患者中的表達及臨床意義.廣州:南方醫科大學, 2014.
17. Gruvberger-Saal SK, Parsons R. Is the small heat shock protein alpha B-crystallin an oncogene? J Clin Invest, 2006, 116(1):30-32.
18. Parcellier A, Schmitt E, Brunet M, et al. Small heat shock proteins HSP27 and alphaB-crystallin:cytoprotective and oncogenic functions. Antioxid Redox Signal, 2005, 7:404-413.
19. Mao YW, Xiang H, Wang J, et al. Human bcl-2 gene attenuates the ability of rabbit lens epithelial cells against H2O2-induced apoptosis through down-regulation of the alpha B-crystallin gene. J Biol Chem, 2001, 276(46):43435-43445.
20. Mao YW, Liu JP, Xiang H, et al. Human alpha A-and alpha B-crystallins bind to Bax and Bcl-X(S) to sequester their translocation during staurosporine-induced apoptosis. Cell Death Differ, 2004, 11(5):512-526.
21. Filomeni G, Aquilano K, Rotilio G, et al. Antiapoptotic response to induced GSH depletion:involvement of heat shock proteins and NF-kappaB activation. Antioxid Redox Signal, 2005, 7(3/4):446-455.
22. Lamoureux F, Thomas C, Yin MJ, et al. Suppression of heat shock protein 27 using OGX-427 induces endoplasmic reticulum stress and potentiates heat shock protein 90 inhibitors to delay castrateresistant prostate cancer. Eur Urol, 2014, 66(1):145-155.
23. 黃曉勇.小熱休克蛋白alpha B-Crystallin (Cryab)在肝癌侵襲轉移中的分子機制及與索拉非尼耐藥研究.上海:復旦大學, 2013.
24. 周娟.人食管癌細胞熱休克蛋白70肽復合物的提取及其抗瘤活性的研究.鄭州:鄭州大學, 2006.
25. Aoyama A, Steiger RH, Frohli E, et al. Expression of alpha B-crystallin in human brain tumors. Int J Cancer, 1993, 55:760-764.
26. Chin D, Boyle GM, Williams RM, et al. Alpha B-crystallin, a new independent marker for poor prognosis in head and neck cancer. Laryngoscope, 2005, 115:1239-1242.
27. 唐奇, 馮振卿. CRYAB基因在HCC中的表達、臨床預后分析及抗凋亡功能研究.南京:南京醫科大學, 2007.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of Alpha B-Crystallin in Colorectal Cancer and Its Clinical Significances

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