Effects of Hypertonic Saline on Levels of Nitric Oxide and Endothelin-1 in Hepatic Ischemia-Reperfusion Injury Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 HUANGTao ¹ , ERQi-dong ¹ , DENGFeng ¹ , BIXu-dong ²

1. Department of General Surgery, Xiqing Hospital of Tianjin, Tianjin 300380, China;
2. Department of General Surgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China;

Corresponding?author：

HUANGTao, Email: htwww2008@126.com

Keywords：

Rat; Liver; Ischemia reperfusion injury; Hypertonic saline; Nitric oxide; Endothelin-1

DOI：

10.7507/1007-9424.20150346

Video：

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Objective To explore the effect of hypertonic saline (HTS) pretreatment on levels of nitric oxide (NO) and endothelin-1(ET-1) and their correlation in hepatic ischemia reperfusion (HIR) injury in rats. Methods The HIR injury models were made by using Pringle, s maneuver in 45 healthy adult male Sprague-Dawley rats, which were randomly divided into three groups (n=15):sham operation (SO) group, HIR group, and HTS group. The animals were killed at 1, 6, and 24 h after reperfusion. The levels of serum NO and ET-1 were measured respectively, the correlation between NO level and ET-1 level at 6 h after reperfusion was analyzed. Results At the time points of 1 h, 6 h, and 24 h after reperfusion, the serum NO levels in the HTS group and HIR group were all significantly lower than those in the SO group (P < 0.01), but the serum ET-1 levels were all significantly higher than those in the so group (P < 0.01). The serum NO levels at the time points of 1 h, 6 h, and 24 h in the HTS group were significantly higher than those at the same time in the HIR group (P < 0.01), but the serum ET-1 levels in the HTS group were significantly lower than those in the HIR group (P < 0.01). At all the time points, every detected goal had more marked level at the time point of 6 h after reperfusion. The NO level was negatively correlated with the ET-1 level. Conclusions HTS could change levels of serum NO and ET-1 after HIR injury, and which has a negative correlation. Its mechanism might probably stimulate serum NO level and reduce the ET-1 level through some way so as to enable both dynamic balance to the benign development direction and achieve a protective effect.

Citation： HUANGTao, ERQi-dong, DENGFeng, BIXu-dong. Effects of Hypertonic Saline on Levels of Nitric Oxide and Endothelin-1 in Hepatic Ischemia-Reperfusion Injury Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(11): 1323-1326. doi: 10.7507/1007-9424.20150346 Copy

1.	?Yada-Langui MM, Anjos-Valotta EA, Sannomiya P, et al. Resuscitation affects microcirculatory polymorphonuclear leukocyte behavior after hemorrhagic shock:role of hypertonic saline and pentoxifylline. Exp Biol Med (Maywood), 2004, 229(7):684-693.
2.	黃濤, 畢旭東.高滲鹽水預處理對肝臟缺血再灌注大鼠血清腫瘤壞死因子-α和IL-10水平的影響.中國全科醫學, 2008, 11(18):1649-1652.
3.	聞靜, 張敬瑩, 倉彥, 等.高滲鹽水預處理在大鼠缺血再灌注心肌損傷中的保護作用.同濟大學學報:醫學版, 2015, 36(2):20-23.
4.	趙晶, 畢旭東, 穆長征, 等.高滲鹽水對大鼠肝臟缺血再灌注損傷的保護作用及其機制.中國老年學雜志, 2013, 33(5):1096-1100.
5.	Scrracion-Lnglott F, Habib N, Mathie RT. Hepatic ischemia reperfusion injury. Am J Surg, 2001, 181(2):160-166.
6.	Fondevila C, Busuttil RW, Kupiec-Weglinski JW. Hepatic ischemia/reperfusion injury-a fresh look. Exp Mol Pathol, 2003, 74(2):86-93.
7.	賈長庫, 陳有科, 韓霖, 等.交替半肝入肝血流阻斷解剖性肝中葉切除.中國普通外科雜志, 2015, 24(1):121-126.
8.	Kohr MJ, Sun J, Aponte A, et al. Simultaneous measurement of protein oxidation and S-nitrosylation during preconditioning and ischemia/reperfusion injury with resin-assisted capture. Circ Res, 2011, 108(4):418-426.
9.	戴勇, 陶立德, 薛同敏, 等.缺血預處理對大鼠肝臟缺血再灌注損傷后細胞凋亡的影響.中國現代普通外科進展, 2015, 18(3):169-172.
10.	張召輝, 王為忠, 李璽, 等. IPC、IPO和IPC-IPO對胰腺冷缺血再灌注損傷保護作用的對比研究.中國普外基礎與臨床雜志, 2011, 18(8):844-848.
11.	Costa FL, Yamaki VN, Gon?alves TB, et al. Combined remote ischemic perconditioning and local postconditioning on liver ischemia-reperfusion injury. J Surg Res, 2014, 192(1):98-102.
12.	李俊, 王健, 李鵬飛, 等.間斷低氧預適應對大鼠肝切除缺血再灌注損傷的保護作用.中國普外基礎與臨床雜志, 2014, 21(10):1284-1286.
13.	Kislin MS, Tiul, kova EI, Samo?lov MO. Dynamics of lipid peroxidation of membranes in cells and mitochondrial fraction of neocortex in non-and preconditioned rats after severe hypobaric hypoxia. Zh Evol Biokhim Fiziol, 2011, 47(2):157-164.
14.	朱耿隆, 蔡潮農, 賈英斌, 等.法舒地爾對阻塞性黃疸大鼠肝缺血再灌注損傷的影響.中華肝膽外科雜志, 2015, 21(1):44-48.
15.	Zhang N, Cheng GY, Liu XZ, et al. Expression of Bcl-2 and NF-κB in brain tissue after acute renal ischemia-reperfusion in rats. Asian Pac J Trop Med, 2014, 7(5):386-389.
16.	張森森, 朱振新, 吳洪坤, 等.高滲鹽水復蘇缺血小腸對小腸免疫屏障和系統炎性反應綜合征的影響.中國實驗診斷學, 2014, 18(2):187-191, 349.
17.	朱高峰, 鄧醫宇, 江穩強, 等.高滲鹽水抑制腫瘤壞死因子-α表達與減輕腦水腫的相關性.實用醫學雜志, 2012, 28(10):1618-1621.
18.	Oddo MS. Effect of mannitol and hypertonic saline on cerebral oxygenation in patients with severe traumatic brain injury and refractory intracranial hypertension. J Neulol Neurosurg Psychiatry, 2009, 80(8):916-920.
19.	李漢臣, 秦兆寅, 紀宗正, 等.高滲鹽水治療對失血性休克鼠網狀內皮細胞功能和易感染性的影響.中國普外基礎與臨床雜志, 2004, 11(1):49-51.
20.	Inagami T, Naruse M, Hoover R. Endothelium as an endocrine organ. Annu Rev Physiol, 1995, 57:171-189.
21.	Carter EP, Sato K, Morio Y, et al. Inhibition of K(Ca) channels restores blunted hypoxic pulmonary vasoconstriction in rats with cirrhosis. Am J Physiol Lung Cell Mol Physiol, 2000, 279(5):L903-L910.
22.	李旭明.烏司他丁對肝缺血再灌注損傷患者血漿一氧化氮/內皮素-1和炎癥細胞因子的調控作用.實用醫學雜志, 2009, 25(8):1293-1295.
23.	袁晟光, 董家鴻.一氧化氮在肝臟病變中的作用.消化外科, 2003, 2(3):209-213.
24.	Nishida J, Mccuskey RS, Mcdonnell D, et al. Protective role of NO in hepatic microcirculatory dysfunction during endotoxemia. Am J Physiol, 1994, 267(6 Pt 1):G1135-G1141.
25.	Urakami A, Todo S, Zhu Y, et al. Attenuation of ischemic liver injury by monoclonal anti-endothelin antibody, AwETN40. J Am Coll Surg, 1997, 185(4):358-364.
26.	郎潔, 張培建.板藍根多糖對大鼠自體肝移植后一氧化氮和內皮素-1的影響.軍事醫學, 2014, 38(10):814-818.

1. ?Yada-Langui MM, Anjos-Valotta EA, Sannomiya P, et al. Resuscitation affects microcirculatory polymorphonuclear leukocyte behavior after hemorrhagic shock:role of hypertonic saline and pentoxifylline. Exp Biol Med (Maywood), 2004, 229(7):684-693.
2. 黃濤, 畢旭東.高滲鹽水預處理對肝臟缺血再灌注大鼠血清腫瘤壞死因子-α和IL-10水平的影響.中國全科醫學, 2008, 11(18):1649-1652.
3. 聞靜, 張敬瑩, 倉彥, 等.高滲鹽水預處理在大鼠缺血再灌注心肌損傷中的保護作用.同濟大學學報:醫學版, 2015, 36(2):20-23.
4. 趙晶, 畢旭東, 穆長征, 等.高滲鹽水對大鼠肝臟缺血再灌注損傷的保護作用及其機制.中國老年學雜志, 2013, 33(5):1096-1100.
5. Scrracion-Lnglott F, Habib N, Mathie RT. Hepatic ischemia reperfusion injury. Am J Surg, 2001, 181(2):160-166.
6. Fondevila C, Busuttil RW, Kupiec-Weglinski JW. Hepatic ischemia/reperfusion injury-a fresh look. Exp Mol Pathol, 2003, 74(2):86-93.
7. 賈長庫, 陳有科, 韓霖, 等.交替半肝入肝血流阻斷解剖性肝中葉切除.中國普通外科雜志, 2015, 24(1):121-126.
8. Kohr MJ, Sun J, Aponte A, et al. Simultaneous measurement of protein oxidation and S-nitrosylation during preconditioning and ischemia/reperfusion injury with resin-assisted capture. Circ Res, 2011, 108(4):418-426.
9. 戴勇, 陶立德, 薛同敏, 等.缺血預處理對大鼠肝臟缺血再灌注損傷后細胞凋亡的影響.中國現代普通外科進展, 2015, 18(3):169-172.
10. 張召輝, 王為忠, 李璽, 等. IPC、IPO和IPC-IPO對胰腺冷缺血再灌注損傷保護作用的對比研究.中國普外基礎與臨床雜志, 2011, 18(8):844-848.
11. Costa FL, Yamaki VN, Gon?alves TB, et al. Combined remote ischemic perconditioning and local postconditioning on liver ischemia-reperfusion injury. J Surg Res, 2014, 192(1):98-102.
12. 李俊, 王健, 李鵬飛, 等.間斷低氧預適應對大鼠肝切除缺血再灌注損傷的保護作用.中國普外基礎與臨床雜志, 2014, 21(10):1284-1286.
13. Kislin MS, Tiul, kova EI, Samo?lov MO. Dynamics of lipid peroxidation of membranes in cells and mitochondrial fraction of neocortex in non-and preconditioned rats after severe hypobaric hypoxia. Zh Evol Biokhim Fiziol, 2011, 47(2):157-164.
14. 朱耿隆, 蔡潮農, 賈英斌, 等.法舒地爾對阻塞性黃疸大鼠肝缺血再灌注損傷的影響.中華肝膽外科雜志, 2015, 21(1):44-48.
15. Zhang N, Cheng GY, Liu XZ, et al. Expression of Bcl-2 and NF-κB in brain tissue after acute renal ischemia-reperfusion in rats. Asian Pac J Trop Med, 2014, 7(5):386-389.
16. 張森森, 朱振新, 吳洪坤, 等.高滲鹽水復蘇缺血小腸對小腸免疫屏障和系統炎性反應綜合征的影響.中國實驗診斷學, 2014, 18(2):187-191, 349.
17. 朱高峰, 鄧醫宇, 江穩強, 等.高滲鹽水抑制腫瘤壞死因子-α表達與減輕腦水腫的相關性.實用醫學雜志, 2012, 28(10):1618-1621.
18. Oddo MS. Effect of mannitol and hypertonic saline on cerebral oxygenation in patients with severe traumatic brain injury and refractory intracranial hypertension. J Neulol Neurosurg Psychiatry, 2009, 80(8):916-920.
19. 李漢臣, 秦兆寅, 紀宗正, 等.高滲鹽水治療對失血性休克鼠網狀內皮細胞功能和易感染性的影響.中國普外基礎與臨床雜志, 2004, 11(1):49-51.
20. Inagami T, Naruse M, Hoover R. Endothelium as an endocrine organ. Annu Rev Physiol, 1995, 57:171-189.
21. Carter EP, Sato K, Morio Y, et al. Inhibition of K(Ca) channels restores blunted hypoxic pulmonary vasoconstriction in rats with cirrhosis. Am J Physiol Lung Cell Mol Physiol, 2000, 279(5):L903-L910.
22. 李旭明.烏司他丁對肝缺血再灌注損傷患者血漿一氧化氮/內皮素-1和炎癥細胞因子的調控作用.實用醫學雜志, 2009, 25(8):1293-1295.
23. 袁晟光, 董家鴻.一氧化氮在肝臟病變中的作用.消化外科, 2003, 2(3):209-213.
24. Nishida J, Mccuskey RS, Mcdonnell D, et al. Protective role of NO in hepatic microcirculatory dysfunction during endotoxemia. Am J Physiol, 1994, 267(6 Pt 1):G1135-G1141.
25. Urakami A, Todo S, Zhu Y, et al. Attenuation of ischemic liver injury by monoclonal anti-endothelin antibody, AwETN40. J Am Coll Surg, 1997, 185(4):358-364.
26. 郎潔, 張培建.板藍根多糖對大鼠自體肝移植后一氧化氮和內皮素-1的影響.軍事醫學, 2014, 38(10):814-818.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Hypertonic Saline on Levels of Nitric Oxide and Endothelin-1 in Hepatic Ischemia-Reperfusion Injury Rats

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