Changes of Peripheral Blood Lymphocyte Subsets of Patients with Primary Hepatocellular Carcinoma Treated with Radiofrequency Ablation_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 LIUJiang-wei ,  HUANGJian-zhao , SUNQian , GOU-xin , LIUYan , ZHAOPeng-wei , ZHANGJia-wei

Department of Hepatobiliary Surgery, Guizhou Provincial People, s Hospita, Guiyang 550002, Guizhou Province, China;

Corresponding?author：

HUANGJian-zhao, Email: huangjzgz@126.com

Keywords：

Primary hepatocellular carcinoma; Radiofrequency ablation; Lymphocyte subsets

DOI：

10.7507/1007-9424.20150305

Video：

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Objective To observe the changes of peripheral blood lymphocyte subsets of patients with primary hepatocellular carcinoma (PHCC) treated with radiofrequency ablation (RFA). Methods The data of 70 cases of hospitalized patients with PHCC that voluntary accepted RFA diagnosed by clinical and pathological in our hospital between July 2011 and December 2014 were collected. According to the numbers of HCC lesions, 70 cases were divided into single focus group (n=41) and multifocal group (n=29). The changes of their immune parameters before and after RFA were analyzed. Results ①The ratioes of peripheral blood CD3⁺/CD19^-, CD3⁺/CD4⁺, CD4⁺/CD8⁺, and NK cells on 7 days and 14 days after RFA treatment of 70 cases were significantly higher than those on 1 day before RFA treatment (P < 0.05). The ratio of CD3⁺/CD8⁺ T cells reduced from 1 day before RFA treatment to 14 days after RFA treatment, but the difference was no statistically significant (P > 0.05).②The changing trend of peripheral blood lymphocyte subsets before and after RFA treatment in single focus group and multifocal group were similar to the above.③Compared with single focus group, the ratioes of peripheral blood CD3⁺/CD19^-, CD3⁺/CD4⁺, CD4⁺/CD8⁺, and NK cells before and after RFA treatment in multifocal group were lower, and the ratio of CD3⁺/CD8⁺ T cells was higher, but the difference were not statistically significant (P > 0.05). Conclusion RFA can not only destroy small PHCC foci, but also to significantly improve immune function and enhance the anti-tumor effect.

Citation： LIUJiang-wei, HUANGJian-zhao, SUNQian, GOU-xin, LIUYan, ZHAOPeng-wei, ZHANGJia-wei. Changes of Peripheral Blood Lymphocyte Subsets of Patients with Primary Hepatocellular Carcinoma Treated with Radiofrequency Ablation. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(10): 1171-1174. doi: 10.7507/1007-9424.20150305 Copy

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5.	陳敏華.微創時代肝癌的射頻消融治療.中華消化外科雜志, 2009, 8(1):4-6.
6.	芶欣, 黃建釗, 孫倩, 等.經皮集束射頻消融治療肝血管瘤的近期療效觀察.廣東醫學, 2011, 32(7):889-890.
7.	Buscarini E, Savoia A, Brambilla G, et al. Radiofrequency thermal ablation of liver tumors. Eur Radiol, 2010, 15(5):884-894.
8.	Truk MJ, Guevara-Pati?o JA, Rizzuto GA, et al. Concomitant tumor immunity to a poorly immunogenic melanoma is prevented by regulatory T cells. J Exp Med, 2011, 200(6):771-782.
9.	Ormandy LA, Hillemann T, Wedemeyer H, et al. Inreased populations of regulatory T cells in peripheral blood of patients with hepatocellular. Cancer Res, 2005, 65(6):2457-2464.
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11.	Smyth MJ, Thia KY, Street SE, C, et al. Defferential tumor surveillance by NK and NKT cells. J Exp Med, 2000, 191(4):661-668.
12.	Liu Y, Poon RT, Hughes J, et al. Chemokine receptions support infiltration of lymphocyte subpopulations in human hepatocelluar carcinoma. Clin Immunol, 2005, 114(2):174-182.
13.	Bentdal O, Fr?land SS, Bosnes V, et al. Alteration in lymphocyte subsets in blood may predict resectibility in carcinoma of cardia or oesophogus. Cancer Letters, 1996, 100(1-2):133-138.
14.	周永昌, 郭萬學.超聲醫學.第4版.北京:北京科學技術文獻出版社, 2003:1622-1624.
15.	陳曉明, 羅鵬飛, 邵培堅, 等.肝癌栓塞化療生存3年以上48例臨床分析.中華放射學雜志, 1995, 29(12):876-877.
16.	Lau WY, Lai EC. The current role of radiofrequency ablation in the management of hepatocellular carcinoma:a systematic review. Ann Surg, 2009, 249(1):20-25.
17.	Buscarini L, Rossi S. Technology for radiofrequency thermal ablation of liver tumors. Semin Laparosc Surg, 1997, 4(2):96-101.
18.	Ali MY, Grimm CF, Ritter M, et a1. Activation of dendritic cells by local ablation of hepatocellular carcinoma. J Hepatol, 2005, 43(5):817-822.
19.	郭穎, 喬正榮.射頻消融治療惡性腫瘤現狀.中華腫瘤防治雜志, 2006, 13(9):703-706.
20.	梁萍, 董寶瑋, 于小玲, 等.超聲引導下經皮微波凝固治療肝癌的臨床應用.中華腫瘤雜志, 1997, 19(6):448-450.
21.	劉君, 蒲永東, 范志忠, 等.西米替丁對大腸癌根治術后患者的免疫調節作用及臨床意義.中華普通外科雜志, 2002, 17(2):72-74.
22.	Seki S, Habu Y, Kawamura T, et al. The liver as a crucial organ in the first line of host defense:the roles of Kupffer cells, natural killer (NK) cells and NK1.1 Ag⁺ T cells in T helper 1 immune responses. Immunol Rev, 2000, 174:35-46.
23.	Smyth MJ, Thia KY, Street SE, et al. Defferential tumor surveillance by NK and NKT cells. J Exp Med, 2000, 191(4):661-668.

1. Choti MA. Surgical management of hepatocellular carcinoma:resection and ablation. J Vasc Interv Radiol, 2002, 13(9 Pt 2):S197-S203.
2. 湯釗猷.原發性肝癌.第2版.上海:上海科學技術出版社, 1999:182-185.
3. 黃建釗, 劉江偉, 孫倩, 等.射頻消融與手術切除治療肝癌的療效比較.廣東醫學雜志, 2012, 33(13):1991-1993.
4. Chan AC, Poon RT, Cheung TT, et al. Survival analysis of re-resection versus radiofrequency ablation for intrahepatic recurrence after hepatectomy for hepatocellular carcinoma. World J Surg, 2012, 36(1):151-156.
5. 陳敏華.微創時代肝癌的射頻消融治療.中華消化外科雜志, 2009, 8(1):4-6.
6. 芶欣, 黃建釗, 孫倩, 等.經皮集束射頻消融治療肝血管瘤的近期療效觀察.廣東醫學, 2011, 32(7):889-890.
7. Buscarini E, Savoia A, Brambilla G, et al. Radiofrequency thermal ablation of liver tumors. Eur Radiol, 2010, 15(5):884-894.
8. Truk MJ, Guevara-Pati?o JA, Rizzuto GA, et al. Concomitant tumor immunity to a poorly immunogenic melanoma is prevented by regulatory T cells. J Exp Med, 2011, 200(6):771-782.
9. Ormandy LA, Hillemann T, Wedemeyer H, et al. Inreased populations of regulatory T cells in peripheral blood of patients with hepatocellular. Cancer Res, 2005, 65(6):2457-2464.
10. Jordan MS, Riley MP, von Boehmer H, et al. Anergy and suppression regulate CD4(+) T cell responses to a self peptide. Eur J Immunol, 2000, 30(1):136-144.
11. Smyth MJ, Thia KY, Street SE, C, et al. Defferential tumor surveillance by NK and NKT cells. J Exp Med, 2000, 191(4):661-668.
12. Liu Y, Poon RT, Hughes J, et al. Chemokine receptions support infiltration of lymphocyte subpopulations in human hepatocelluar carcinoma. Clin Immunol, 2005, 114(2):174-182.
13. Bentdal O, Fr?land SS, Bosnes V, et al. Alteration in lymphocyte subsets in blood may predict resectibility in carcinoma of cardia or oesophogus. Cancer Letters, 1996, 100(1-2):133-138.
14. 周永昌, 郭萬學.超聲醫學.第4版.北京:北京科學技術文獻出版社, 2003:1622-1624.
15. 陳曉明, 羅鵬飛, 邵培堅, 等.肝癌栓塞化療生存3年以上48例臨床分析.中華放射學雜志, 1995, 29(12):876-877.
16. Lau WY, Lai EC. The current role of radiofrequency ablation in the management of hepatocellular carcinoma:a systematic review. Ann Surg, 2009, 249(1):20-25.
17. Buscarini L, Rossi S. Technology for radiofrequency thermal ablation of liver tumors. Semin Laparosc Surg, 1997, 4(2):96-101.
18. Ali MY, Grimm CF, Ritter M, et a1. Activation of dendritic cells by local ablation of hepatocellular carcinoma. J Hepatol, 2005, 43(5):817-822.
19. 郭穎, 喬正榮.射頻消融治療惡性腫瘤現狀.中華腫瘤防治雜志, 2006, 13(9):703-706.
20. 梁萍, 董寶瑋, 于小玲, 等.超聲引導下經皮微波凝固治療肝癌的臨床應用.中華腫瘤雜志, 1997, 19(6):448-450.
21. 劉君, 蒲永東, 范志忠, 等.西米替丁對大腸癌根治術后患者的免疫調節作用及臨床意義.中華普通外科雜志, 2002, 17(2):72-74.
22. Seki S, Habu Y, Kawamura T, et al. The liver as a crucial organ in the first line of host defense:the roles of Kupffer cells, natural killer (NK) cells and NK1.1 Ag⁺ T cells in T helper 1 immune responses. Immunol Rev, 2000, 174:35-46.
23. Smyth MJ, Thia KY, Street SE, et al. Defferential tumor surveillance by NK and NKT cells. J Exp Med, 2000, 191(4):661-668.

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Changes of Peripheral Blood Lymphocyte Subsets of Patients with Primary Hepatocellular Carcinoma Treated with Radiofrequency Ablation

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