Regulation Role ofβ<sub>2</sub> Adrenoceptor on Alveolar Fluid Clearance of Severe Acute Pancreatitis in Rats Model_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 XUJin , LIUZong-jian , LIANGHan-li

Department of Pancreatic Surgery, Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China;

Corresponding?author：

XUJin, Email: xujinwang@163.com

Keywords：

Severe acute pancreatitis; Alveolar fluid clearance; Pulmonary edema; β₂ adrenoceptor

DOI：

10.7507/1007-9424.20140332

Video：

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Objective To discuss the effect ofβ₂ adrenoceptor on the alveolar fluid clearance (AFC) of the rats with severe acute pancreatitis (SAP). Methods SD rats was made to SAP model by injecting taurocholate into biliary-pancreatic duct.These rats were randomly divided into sham operation group and SAP group, the SAP group was divided into subgroups of SAP-4 h and SAP-24 h according to the sampling time after making model.The wet-to-dry ratio, AFC, and AFC affected byβ₂ adrenoceptor agonist-terbutaline or inhibitor-propranolol were measured in the bilateral lungs.β₂ adrenoceptor mRNA expression in the lungs tissues was measured by real-time-PCR. Results Compared with the sham operation group, the wet-to-dry ratio was significantly decreased (P < 0.05) and the AFC was significantly increased in the subgroup of SAP-4 h or SAP-24 h (P < 0.05), β₂ adrenoceptor agonist-terbutaline couldn't increase the AFC of the subgroup of SAP-4 h or SAP-24 h (P > 0.05), inhibitor-propranolol could decrease AFC of subgroup of SAP-4 h or SAP-24 h (P < 0.05).β₂ adrenoceptor mRNA was decreased in the subgroup of SAP-4 h or SAP-24 h as compared with the sham operation group (P < 0.05). Conclusions Bilateral lung liquid volome induced by SAP is less than the normal lung, AFC is increased in the early period of SAP but decreased in the late period.when the lung injury happens, β₂ adrenoceptor might modulate AFC in rats of SAP model.The mechanism of lung injury of SAP is so complex that we need more experiments to be done.

Citation： XUJin, LIUZong-jian, LIANGHan-li. Regulation Role ofβ₂ Adrenoceptor on Alveolar Fluid Clearance of Severe Acute Pancreatitis in Rats Model. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(11): 1391-1395. doi: 10.7507/1007-9424.20140332 Copy

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6.	唐中建, 王金龍, 羅燕, 等.重癥急性胰腺炎早期ICU綜合救治模式探討[J].中國普外基礎與臨床雜志, 2008, 15(5):369-370.
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10.	Wang Z, Xu J, Ma G, et al.Chronic pulmonary artery occlusion increases alveolar fluid clearance in rats[J].J Thorac Cardiovasc Surg, 2007, 134(5):1213-1219.
11.	K?ksoy FN, Yankol Y, Oran ES, et al.Acute lung injury in patients with severe acute pancreatitis[J].Turk J Gastroenterol, 2013, 24(5):495-501.
12.	De Campos T, Deree J, Coimbra R.From acute pancreatitis to end-organ injury:mechanisms of acute lung injury[J].Surg Infect (Larchmt), 2007, 8(1):107-120.
13.	Raghu M, Wig JD, Kochhar R, et al.Lung complications in acute pancreatitis[J].JOP, 2007, 8(2):177-185.
14.	Brown LM, Calfee CS, Howard JP, et al.Comparison of thermodilution measured extravascular lung water with chest radiographic assessment of pulmonary oedema in patients with acute lung injury[J].Ann Intensive Care, 2013, 3(1):25.
15.	da Costa DW, Boerma D, van Santvoort HC, et al.Staged multidisciplinary step-up management for necrotizing pancreatitis[J].Br J Surg, 2014, 101(1):e65-e79.
16.	Howard M, Roux J, Iles KE, et al.Activation of the heat shock response attenuates the interleukin 1β-mediated inhibition of the amiloride-sensitive alveolar epithelial ion transport[J].Shock, 2013, 39(2):189-196.
17.	Roux J, Mcnicholas CM, Carles M, et al.IL-8 inhibits cAMP-stimulated alveolar epithelial fluid transport via a GRK2/PI3K-dependent mechanism[J].FASEB J, 2013, 27(3):1095-1106.
18.	Lee JW, Krasnodembskaya A, Mckenna DH, et al.Therapeutic effects of human mesenchymal stem cells in ex vivo human lungs injured with live bacteria[J].Am J Respir Crit Care Med, 2013, 187(7):751-760.
19.	Matthay MA, Thompson BT, Read EJ, et al.Therapeutic potential of mesenchymal stem cells for severe acute lung injury[J].Chest, 2010, 138(4):965-972.
20.	Downs CA, Kreiner LH, Trac DQ, et al.Acute effects of cigarette smoke extract on alveolar epithelial sodium channel activity and lung fluid clearance[J].Am J Respir Cell Mol Biol, 2013, 49(2):251-259.
21.	Wang Q, Lian QQ, Li R, et al.Lipoxin a(4) activates alveolar epithelial sodium channel, Na, K-ATPase, and increases alveolar fluid clearance[J].Am J Respir Cell Mol Biol, 2013, 48(5):610-618.
22.	Paolillo S, Pellegrino R, Salvioni E, et al.Role of alveolarβ₂-adrenergic receptors on lung fluid clearance and exercise ventilation in healthy humans[J].PLoS One, 2013, 8(4):e61877.
23.	Cross LJ, Matthay MA.Biomarkers in acute lung injury:insights into the pathogenesis of acute lung injury[J].Crit Care Clin, 2011, 27(2):355-377.
24.	Zemans RL, Matthay M.Bench-to-bedside review:The role of the alveolar epithelium in the resolution of pulmonary edema in acute lung injury[J].Crit Care, 2004, 8(6):469-477.
25.	張蔚, 江山平, 李元廣, 等.C57BL/6哮喘小鼠細胞內鎂含量與肺組織β₂受體mRNA表達的相關性研究[J].中國病理生理雜志, 2008, 24(7):1394-1398.

1. ?Adam F, Bor C, Uyar M, et al.Severe acute pancreatitis admitted to intensive care unit:SOFA is superior to Ranson's criteria and APACHEⅡin determining prognosi[J].Turk J Gastroenterol, 2013, 24(5):430-435.
2. 張圣道, 雷若慶.指南后時代重癥急性胰腺炎外科治療的一些關鍵問題[J].中國普外基礎與臨床雜志, 2007, 14(4):381-382.
3. Banks PA, Bollen TL, Dervenis C, et al.Classification of acute pancreatitis-2012:revision of the Atlanta classification and definitions by international consensus[J].Gut, 2013, 62(1):102-111.
4. Akbarshahi H, Rosendahl AH, Westergren-Thorsson G, et al.Acute lung injury in acute pancreatitis-Awaiting the big leap[J].Respir Med, 2012, 106(9):1199-1210.
5. Bryner BS, Smith C, Cooley E, et al.Extracorporeal life support for pancreatitis-induced acute respiratory distress syndrome[J].Ann Surg, 2012, 256(6):1073-1077.
6. 唐中建, 王金龍, 羅燕, 等.重癥急性胰腺炎早期ICU綜合救治模式探討[J].中國普外基礎與臨床雜志, 2008, 15(5):369-370.
7. Mentula P, Lepp?niemi A1.Position paper:timely interventions in severe acute pancreatitis are crucial for survival[J].World J Emerg Surg, 2014, 9(1):15.
8. Liu G, Zhang J, Chen H, et al.Effects and mechanisms of alveolar typeⅡepithelial cell apoptosis in severe pancreatitis-induced acute lung injury[J].Exp Ther Med, 2014, 7(3):565-572.
9. Mofidi R, Duff MD, Wigmore SJ, et al.Association between early systemic inflammatory response, severity of multiorgan dysfunction and death in acute pancreatitis[J].Br J Surg, 2006, 93(6):738-744.
10. Wang Z, Xu J, Ma G, et al.Chronic pulmonary artery occlusion increases alveolar fluid clearance in rats[J].J Thorac Cardiovasc Surg, 2007, 134(5):1213-1219.
11. K?ksoy FN, Yankol Y, Oran ES, et al.Acute lung injury in patients with severe acute pancreatitis[J].Turk J Gastroenterol, 2013, 24(5):495-501.
12. De Campos T, Deree J, Coimbra R.From acute pancreatitis to end-organ injury:mechanisms of acute lung injury[J].Surg Infect (Larchmt), 2007, 8(1):107-120.
13. Raghu M, Wig JD, Kochhar R, et al.Lung complications in acute pancreatitis[J].JOP, 2007, 8(2):177-185.
14. Brown LM, Calfee CS, Howard JP, et al.Comparison of thermodilution measured extravascular lung water with chest radiographic assessment of pulmonary oedema in patients with acute lung injury[J].Ann Intensive Care, 2013, 3(1):25.
15. da Costa DW, Boerma D, van Santvoort HC, et al.Staged multidisciplinary step-up management for necrotizing pancreatitis[J].Br J Surg, 2014, 101(1):e65-e79.
16. Howard M, Roux J, Iles KE, et al.Activation of the heat shock response attenuates the interleukin 1β-mediated inhibition of the amiloride-sensitive alveolar epithelial ion transport[J].Shock, 2013, 39(2):189-196.
17. Roux J, Mcnicholas CM, Carles M, et al.IL-8 inhibits cAMP-stimulated alveolar epithelial fluid transport via a GRK2/PI3K-dependent mechanism[J].FASEB J, 2013, 27(3):1095-1106.
18. Lee JW, Krasnodembskaya A, Mckenna DH, et al.Therapeutic effects of human mesenchymal stem cells in ex vivo human lungs injured with live bacteria[J].Am J Respir Crit Care Med, 2013, 187(7):751-760.
19. Matthay MA, Thompson BT, Read EJ, et al.Therapeutic potential of mesenchymal stem cells for severe acute lung injury[J].Chest, 2010, 138(4):965-972.
20. Downs CA, Kreiner LH, Trac DQ, et al.Acute effects of cigarette smoke extract on alveolar epithelial sodium channel activity and lung fluid clearance[J].Am J Respir Cell Mol Biol, 2013, 49(2):251-259.
21. Wang Q, Lian QQ, Li R, et al.Lipoxin a(4) activates alveolar epithelial sodium channel, Na, K-ATPase, and increases alveolar fluid clearance[J].Am J Respir Cell Mol Biol, 2013, 48(5):610-618.
22. Paolillo S, Pellegrino R, Salvioni E, et al.Role of alveolarβ₂-adrenergic receptors on lung fluid clearance and exercise ventilation in healthy humans[J].PLoS One, 2013, 8(4):e61877.
23. Cross LJ, Matthay MA.Biomarkers in acute lung injury:insights into the pathogenesis of acute lung injury[J].Crit Care Clin, 2011, 27(2):355-377.
24. Zemans RL, Matthay M.Bench-to-bedside review:The role of the alveolar epithelium in the resolution of pulmonary edema in acute lung injury[J].Crit Care, 2004, 8(6):469-477.
25. 張蔚, 江山平, 李元廣, 等.C57BL/6哮喘小鼠細胞內鎂含量與肺組織β₂受體mRNA表達的相關性研究[J].中國病理生理雜志, 2008, 24(7):1394-1398.

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Regulation Role ofβ₂ Adrenoceptor on Alveolar Fluid Clearance of Severe Acute Pancreatitis in Rats Model

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Regulation Role ofβ₂ Adrenoceptor on Alveolar Fluid Clearance of Severe Acute Pancreatitis in Rats Model