Intermittent Hypoxic Preconditioning Alleviates Hepatic Ischemia Reperfusion Injury after Partial Hepatectomy in Rats by Antioxidant Pathways_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 李俊 ^1,2 , 王健 ¹ , 李鵬飛 ¹ , 韓效帆 ¹ , 朱世春 ¹ , 李廣 ¹ ,  張培建 ¹

1. ;
2. ;

Corresponding?author：

張培建, Email: yzu.edu.pjz@163.com

Keywords：

間斷低氧預適應; 缺血再灌注損傷; 肝臟切除; 超氧化物歧化酶; 丙二醛

DOI：

10.7507/1007-9424.20140305

Video：

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目的探討間斷低氧預適應對大鼠肝大部切除術后殘余肝臟合并缺血再灌注引發過氧化損傷的保護作用。方法 78只SD大鼠，用SPSS軟件將其隨機分為4組：假手術組（SO組，n=6）、肝切除組（PH組，n=24）、肝切除合并缺血再灌注損傷組（IR組，n=24）和間斷低氧預適應組（IHP組，n=24）。以無創傷血管夾阻斷IR組大鼠入肝血流后切除肝臟的左葉和中葉（約占全肝的70%），20 min后開放入肝血流，殘余肝臟發生了缺血再灌注損傷。將IHP組大鼠暴露于10%的低氧環境中，每日持續1 h，連續進行1周，最后1次低氧暴露后行肝切除術（同IR組）。SO組大鼠在術后2 h取材檢測，其余各組分別于術后2、6、12及24 h進行檢測。檢測血清轉氨酶（ALT、AST）水平和肝勻漿組織中超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量。結果術后2 h，PH組、IR組和IHP組大鼠血清ALT和AST水平均高于SO組（P<0.05）。在術后6、12和24 h，IHP組大鼠血清ALT和AST均高于PH組，但低于IR組（均P<0.05）。與IR組相比，IHP組大鼠術后各時間點殘余肝臟中SOD活性明顯升高，而MDA含量則顯著降低（均P<0.05）。結論間斷低氧預適應對殘余肝臟缺血再灌注損傷具有保護作用，其機理可能與提高肝臟的抗氧化能力有關。

Citation： 李俊, 王健, 李鵬飛, 韓效帆, 朱世春, 李廣, 張培建. Intermittent Hypoxic Preconditioning Alleviates Hepatic Ischemia Reperfusion Injury after Partial Hepatectomy in Rats by Antioxidant Pathways. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(10): 1284-1286. doi: 10.7507/1007-9424.20140305 Copy

1.	Papadopoulos D, Siempis T, Theodorakou E, et al. Hepatic ischemia and reperfusion injury and trauma:current concepts[J]. Arch Trauma Res, 2013, 2(2):63-70.
2.	楊明峰, 張顏波, 孫保亮, 等.低氧預適應小鼠腦勻漿液提取液對大鼠鼠胚海馬神經元缺氧復氧后神經細胞的影響[J].中華神經醫學雜志, 2009, 8(11):1094-1097.
3.	Wang ZH, Chen YX, Zhang CM, et al. Intermittent hypobaric hypoxia improves postischemic recovery of myocardial contractile function via redox signaling during early reperfusion[J]. Am J Physiol Heart Circ Physiol, 2011, 301(4):H1695-H1705.
4.	Xu WQ, Yu Z, Xie Y, et al. Therapeutic effect of intermittent hypobaric hypoxia on myocardial infarction in rats[J]. Basic Res Cardiol, 2011, 106(3):329-342.
5.	Sasaki H, Fukuda S, Otani H, et al. Hypoxic preconditioning triggers myocardial angiogenesis:a novel approach to enhance contractile functional reserve in rat with myocardial infarction[J]. J Mol Cell Cardiol, 2002, 34(3):335-348.
6.	Maslov LN, Lishmanov IuB, Kolar F, et al. Hypoxic preconditioning is a phenomenon increasing tolerance of cardiomyocytes to hypoxia-reoxygenation[J]. Ross Fiziol Zh Im I M Sechenova, 2010, 96(12):1170-1189.
7.	Kaminski A, Kasch C, Zhang L, et al. Endothelial nitric oxide synthase mediates protective effects of hypoxic preconditioning in lungs[J]. Respir Physiol Neurobiol, 2007, 155(3):280-285.
8.	Choukèr A, Ohta A, Martignoni A, et al. In vivo hypoxic precon ditioning protects from warm liver ischemia-reperfusion injury through the adenosine A2B receptor[J]. Transplantation, 2012, 94(9):894-902.
9.	周斌, 張培建, 李勇, 等. VEGF參與低氧預適應對移植肝肝內膽管上皮細胞的保護作用[J].中國普外基礎與臨床雜志, 2009, 16(5):365-369.
10.	Elias-Miró M, Jiménez-Castro MB, Rodés J, et al. Current knowledge on oxidative stress in hepatic ischemia/reperfusion[J]. Free Radic Res, 2013, 47(8):555-568.
11.	Klune JR, Tsung A. Molecular biology of liver ischemia/reper-fusion injury:established mechanisms and recent advancements[J]. Surg Clin North Am, 2010, 90(4):665-677.
12.	Cesaratto L, Vascotto C, Calligaris S, et al. The importance of redox state in liver damage[J]. Ann Hepatol, 2004, 3(3):86-92.
13.	Tanrikulu Y, Sahin M, Kismet K, et al. The protective effect of diosmin on hepatic ischemia reperfusion injury:an experimental study[J]. Bosn J Basic Med Sci, 2013, 13(4):218-224.
14.	Yu S, Zheng J, Jiang Z, et al. Protective effect of N-acetylserotonin against acute hepatic ischemia-reperfusion injury in mice[J]. Int J Mol Sci, 2013, 14(9):17680-17693.
15.	梅丹, 徐斌, 羅軍, 等.急性重復缺氧對大鼠腦抗氧化活性和脂質過氧化反應的影響[J].中國臨床康復, 2003, 7(31):4194-4195.
16.	李潔, 張耀斌.不同低氧訓練模式對大鼠力竭運動后骨骼肌線粒體抗氧化能力及呼吸鏈酶復合體活性的影響[J].生理學報, 2011, 63(1):55-61.
17.	Kislin MS, Tiul'kova EI, Samo?lov MO. Dynamics of lipid peroxidation of membranes in cells and mitochondrial fraction of neocortex in non-and preconditioned rats after severe hypobaric hypoxia[J]. Zh Evol Biokhim Fiziol, 2011, 47(2):157-164.

1. Papadopoulos D, Siempis T, Theodorakou E, et al. Hepatic ischemia and reperfusion injury and trauma:current concepts[J]. Arch Trauma Res, 2013, 2(2):63-70.
2. 楊明峰, 張顏波, 孫保亮, 等.低氧預適應小鼠腦勻漿液提取液對大鼠鼠胚海馬神經元缺氧復氧后神經細胞的影響[J].中華神經醫學雜志, 2009, 8(11):1094-1097.
3. Wang ZH, Chen YX, Zhang CM, et al. Intermittent hypobaric hypoxia improves postischemic recovery of myocardial contractile function via redox signaling during early reperfusion[J]. Am J Physiol Heart Circ Physiol, 2011, 301(4):H1695-H1705.
4. Xu WQ, Yu Z, Xie Y, et al. Therapeutic effect of intermittent hypobaric hypoxia on myocardial infarction in rats[J]. Basic Res Cardiol, 2011, 106(3):329-342.
5. Sasaki H, Fukuda S, Otani H, et al. Hypoxic preconditioning triggers myocardial angiogenesis:a novel approach to enhance contractile functional reserve in rat with myocardial infarction[J]. J Mol Cell Cardiol, 2002, 34(3):335-348.
6. Maslov LN, Lishmanov IuB, Kolar F, et al. Hypoxic preconditioning is a phenomenon increasing tolerance of cardiomyocytes to hypoxia-reoxygenation[J]. Ross Fiziol Zh Im I M Sechenova, 2010, 96(12):1170-1189.
7. Kaminski A, Kasch C, Zhang L, et al. Endothelial nitric oxide synthase mediates protective effects of hypoxic preconditioning in lungs[J]. Respir Physiol Neurobiol, 2007, 155(3):280-285.
8. Choukèr A, Ohta A, Martignoni A, et al. In vivo hypoxic precon ditioning protects from warm liver ischemia-reperfusion injury through the adenosine A2B receptor[J]. Transplantation, 2012, 94(9):894-902.
9. 周斌, 張培建, 李勇, 等. VEGF參與低氧預適應對移植肝肝內膽管上皮細胞的保護作用[J].中國普外基礎與臨床雜志, 2009, 16(5):365-369.
10. Elias-Miró M, Jiménez-Castro MB, Rodés J, et al. Current knowledge on oxidative stress in hepatic ischemia/reperfusion[J]. Free Radic Res, 2013, 47(8):555-568.
11. Klune JR, Tsung A. Molecular biology of liver ischemia/reper-fusion injury:established mechanisms and recent advancements[J]. Surg Clin North Am, 2010, 90(4):665-677.
12. Cesaratto L, Vascotto C, Calligaris S, et al. The importance of redox state in liver damage[J]. Ann Hepatol, 2004, 3(3):86-92.
13. Tanrikulu Y, Sahin M, Kismet K, et al. The protective effect of diosmin on hepatic ischemia reperfusion injury:an experimental study[J]. Bosn J Basic Med Sci, 2013, 13(4):218-224.
14. Yu S, Zheng J, Jiang Z, et al. Protective effect of N-acetylserotonin against acute hepatic ischemia-reperfusion injury in mice[J]. Int J Mol Sci, 2013, 14(9):17680-17693.
15. 梅丹, 徐斌, 羅軍, 等.急性重復缺氧對大鼠腦抗氧化活性和脂質過氧化反應的影響[J].中國臨床康復, 2003, 7(31):4194-4195.
16. 李潔, 張耀斌.不同低氧訓練模式對大鼠力竭運動后骨骼肌線粒體抗氧化能力及呼吸鏈酶復合體活性的影響[J].生理學報, 2011, 63(1):55-61.
17. Kislin MS, Tiul'kova EI, Samo?lov MO. Dynamics of lipid peroxidation of membranes in cells and mitochondrial fraction of neocortex in non-and preconditioned rats after severe hypobaric hypoxia[J]. Zh Evol Biokhim Fiziol, 2011, 47(2):157-164.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Intermittent Hypoxic Preconditioning Alleviates Hepatic Ischemia Reperfusion Injury after Partial Hepatectomy in Rats by Antioxidant Pathways

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