Improvement of Method for Primary Culture of Vascular Smooth Muscle Cells of Diabetic Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 YANGTing , JINSong ,  LIUYan-dong

Department of Vascular Surgery, The First Hospital Affiliated to Jiamusi University, Jiamusi 154000, Heilongjiang Province, China;

Corresponding?author：

LIUYan-dong, Email: liuyandong867719@126.com

Keywords：

Diabetes rat; Aortic vascular smooth muscle cell; Cultured in vitro

DOI：

10.7507/1007-9424.20140040

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To explore a simple, efficient method for primary culture in vascular smooth muscle cells of diabetic rat, to establish a long-term stable diabetic vascular smooth muscle cell model in vitro, and lay the foundation for the study of diabetes chronic vascular lesions. Methods Twenty diabetic Wister rats models were self-made by using streptozotocin intraperitoneal injection plus high fat and sugar feeding, the vascular smooth muscle cells in vitro was cultured by a modified enzymatic digestion. Results The diabetic rat models were successfully established, the vascular smooth muscle cells cultured in vitro by modified enzyme digestion grew fast, the cell survival was 96%, it could meet the requirements for cell experiment in vitro. Conclusion Comparing with the traditional method, the modified enzymatic digestion method is simple, economic, high survival rate.

Citation： YANGTing, JINSong, LIUYan-dong. Improvement of Method for Primary Culture of Vascular Smooth Muscle Cells of Diabetic Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(2): 173-176. doi: 10.7507/1007-9424.20140040 Copy

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16.	嚴利君, 謝自敬, 樊勇.高血糖對實驗性糖尿病大鼠心臟血管病變的作用[J].武警醫學, 2004, 15(4):266-269.
17.	胡向陽, 黃曉明, 劉乃豐, 等.高度糖基化終產物對大鼠主動脈平滑肌細胞G-蛋白亞基基因表達的影響[J].東南大學學報:醫學版, 2004, 23(3):145-147.
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19.	盧圣棟.現代分子生物學實驗技術[M].第2版.北京:中國協和醫科大學出版社, 1999:439-441.

1. 李萍, 鄭蓮星, 王伶, 等.反復貼塊法培養大鼠主動脈平滑肌細胞[J].中國組織工程研究與臨床康復, 2007, 11(46):9319-9321.
2. 毛昱嘉, 王文杰.胰酶消化法培養大鼠胸主動脈平滑肌細胞[J].中國動脈硬化雜志, 2004, 12(6):729-731.
3. 王忠太, 馬佳玲.組織塊貼壁法培養大鼠主動脈平滑肌細胞及鑒定[J].衛生職業教育, 2010, 28(3):97-98.
4. 姚飛.常用的細胞培養方法、污染及污染處理[J].科技信息, 2009, (7):422, 447.
5. 王立新, 楊朝霞.動物細胞培養及應用[J].黃牛雜志, 2000, 26(4):45-48.
6. 司徒鎮強.細胞培養[M].第2版.北京:世界圖書出版社, 2007:196.
7. 司徒鎮強.細胞培養[M].第2版.北京:世界圖書出版社, 2007:222-223.
8. Laakso M.Hyperglycemia and cardiovascular disease in type 2 diabetes[J].Diabetes, 1999, 48(5):937-942.
9. Ross R.The pathogenesis of atherosclerosis:a perspective for the 1990s[J].Nature, 1993, 362(6423):801-809.
10. Overbeck HW, Wu G, Durham JP.Humoral factor (s) in hypertensive rats trophic for cultured aortic smooth muscle cells[J].Am J Physiol, 1995, 268(2 Pt 2):767-772.
11. 倪偉, 劉濤, 王浩宇, 等.酶聯合消化法培養小鼠主動脈平滑肌原代細胞[J].西部醫學, 2013, 25(7):968-969.
12. 辛毅, 許秀芳, 黃益民, 等.乳小鼠心肌成纖維細胞和心肌細胞的分離培養及熒光鑒定[J].新鄉醫學院學報, 2011, 28(5):541-547.
13. 楊東偉, 張清華, 蔣知新, 等.大鼠胸主動脈平滑肌細胞膠原酶解法培養與鑒定[J].中國現代醫學雜志, 2009, 19(9):1333-1335.
14. 涂永生, 黃紅林, 朱炳陽, 等.Ⅱ型膠原酶/彈性蛋白酶消化法培養大鼠血管平滑肌細胞[J].中國動脈硬化雜志, 2001, 9(5):438-440.
15. 張鵬, 林福玉, 張迎梅, 等.小鼠主動脈血管平滑肌原代細胞的分離培養及鑒定[J].生物技術通訊, 2010, 21(1):54-57, 79.
16. 嚴利君, 謝自敬, 樊勇.高血糖對實驗性糖尿病大鼠心臟血管病變的作用[J].武警醫學, 2004, 15(4):266-269.
17. 胡向陽, 黃曉明, 劉乃豐, 等.高度糖基化終產物對大鼠主動脈平滑肌細胞G-蛋白亞基基因表達的影響[J].東南大學學報:醫學版, 2004, 23(3):145-147.
18. 司徒鎮強.細胞培養[M].第2版.北京:世界圖書出版社, 2007:54-57.
19. 盧圣棟.現代分子生物學實驗技術[M].第2版.北京:中國協和醫科大學出版社, 1999:439-441.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Improvement of Method for Primary Culture of Vascular Smooth Muscle Cells of Diabetic Rats

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