Study on Mechanism of Invasion of CD133 Positive Population in Gallbladder Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 YUYuan-lin ^1,2 , JIANGBo-jian ¹ , LURui-qi ¹ , WANGShou-lian ^1,2 ,  YUJi-wei ¹

1. Department of General Surgery, No. 3 People's Hospital, Shanghai Jiao-tong University School of Medicine, Shanghai 201900, China;
2. School of Postgraduate, Bengbu Medical College, Bengbu 233030, Anhui Province, China;

Corresponding?author：

YUJi-wei, Email: jiweiyu919@hotmail.com

Keywords：

Gallbladder cancer; Invasion; CD133; SDF-1α/CXCR4

DOI：

10.7507/1007-9424.20140036

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To study the mechanism of invasion of CD133 positive population in gallbladder cancer. Methods The invasive abilities of the CD133 positive cells and the CD133 negative cells were detected by Transwell.The CXCR4 mRNA and protein in the CD133 positive cells and the CD133 negative cells were detected by the semi-quanti-tative RT-PCR, Western blot method, and immunofluorescence, respectively.SDF-1αand AMD3100 were respectively used to stimulate/inhibit the GBC-SD cells.The invasive ability and the migration force were detected in the CD133 posi-tive cells and the CD133 negative cells.The expressions CD133 mRNA and protein of the GBC-SD cells were detected by semi-quantitative RT-PCR and Western blot method, respectively. Results ①The number of invasion cells in the CD133 positive cells was significantly more than that in the CD133 negative cells (23.78±8.74 versus 6.56±3.09, P=0.000 7).②The fluorescent protein of CXCR4 in the CD133 positive cells was stronger than that in the CD133 negative cells.The expression of CXCR4 mRNA in the CD133 positive cells was significantly higher than that in the CD133 negative cells (0.642 4±0.020 4 versus 0.335 9±0.043 2, P=0.004).The expression of CXCR4 protein in the CD133 positive cells was significantly higher than that in the CD133 negative cells (0.765 0±0.106 6 versus 0.409 4±0.019 5, P=0.013).③In the CD133 positive cells, compared with the control group, the number of invasion cells was significantly increased in the SDF-1αgroup (62.89±15.27 versus 23.78±8.74, P=0.000 6) and decreased in the AMD3100 group (10.33±2.00 versus 23.78±8.74, P=0.000 2).In the CD133 negative cells, compared with the control group, the number of invasion cells was not significant change in the SDF-1αgroup (6.89±4.23 versus 6.59±3.09, P=0.41) and in the AMD3100 group (6.11±2.67 versus 6.59±3.09, P=0.38), respectively.④In the CD133 positive cells, compared with the control group, the number of migration cells was significantly increased in the SDF-1αgroup (74.56±15.80 versus 35.56±10.97, P=0.000 3) and decreased in the AMD3100 group (12.67±2.40 versus 35.56±10.97, P=0.000 2).In the CD133 negative cells, compared with the control group, the number of migration cells was not significant change in the SDF-1αgroup (9.78±2.04 versus 9.56±1.74, P=0.43) and in the AMD3100 group (9.54±1.74 versus 9.56±1.74, P=0.42).⑤In the GBC-SD cells, compared with the control group, the CD133 mRNA was significantly increased in the SDF-1αgroup (0.626 5±0.048 7 versus 0.450 0±0.024 3, P=0.004) and decreased in the AMD3100 group (0.359 3±0.047 3 versus 0.450 0±0.024 3, P=0.011);the CD133 protein was significantly increased in the SDF-1αgroup (0.508 9±0.020 7 versus 0.440 9±0.013 0, P=0.016) and decreased in the AMD3100 group (0.317 7±0.013 7 versus 0.440 9±0.013 0, P=0.004). Conclusion The high invasion ability of CD133 positive population in gallbladder cancer might be due to the high expression of CXCR4.

Citation： YUYuan-lin, JIANGBo-jian, LURui-qi, WANGShou-lian, YUJi-wei. Study on Mechanism of Invasion of CD133 Positive Population in Gallbladder Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(2): 156-161. doi: 10.7507/1007-9424.20140036 Copy

1.	Boutros C, Gary M, Baldwin K, et al.Gallbladder cancer:past, present and an uncertain future[J].Surg Oncol, 2012, 21(4):183-191.
2.	Sugihara E, Saya H.Complexity of cancer stem cells[J].Int J Cancer, 2013, 132(6):1249-1259.
3.	俞遠林, 姜波健, 俞繼衛, 等.免疫磁珠法分離膽囊癌CD133陽性細胞及生物學行為的研究[J].中華實驗外科雜志, 2012, 30(8):1652-1655.
4.	Domanska UM, Timmer-Bosscha H, Nagengast WB, et al.CXCR4 inhibition with AMD3100 sensitizes prostate cancer to docetaxel chemotherapy[J].Neoplasia, 2012, 14(8):709-718.
5.	陸瑞祺, 吳巨鋼, 周國才, 等.胃癌C D133陽性細胞亞群腫瘤起始細胞樣特性的檢測[J].中華胃腸外科雜志, 2012, 15(2):174-179.
6.	Ricci-Vitiani L, Lombardi DG, Pilozzi E, et al.Identification and expansion of human colon-cancer-initiating cells[J].Nature, 2007, 445(7123):111-115.
7.	Galli R, Binda E, Orfanelli U, et al.Isolation and characterization of tumorigenic, stem-like neural precursors from human glioblastoma[J].Cancer Res, 2004, 64(19):7011-7021.
8.	Bao S, Wu Q, McLendon RE, et al.Glioma stem cells promote radioresistance by preferential activation of the DNA damage res-ponse[J].Nature, 2006, 444(7120):756-760.
9.	Yin S, Li J, Hu C, et al.C D133 positive hepatocellular carcinoma cells possess high capacity for tumorigenicity[J].Int J Cancer, 2007, 120(7):1444-1450.
10.	Müller A, Homey B, Soto H, et al.Involvement of chemokine receptors in breast cancer metastasis[J].Nature, 2001, 410(6824):50-56.
11.	Saur D, Seidler B, Schneider G, et al.CXCR4 expression incr-eases liver and lung metastasis in a mouse model of pancreatic cancer[J].Gastroenterology, 2005, 129(4):1237-1250.
12.	Schimanski CC, Schwald S, Simiantonaki N, et al.Effect of chemokine receptors CXCR4 and CCR7 on the metastatic behaviorof human colorectal cancer[J].Clin Cancer Res, 2005, 11(5):1743-1750.
13.	Lee HJ, Lee K, Lee DG, et al.Chemokine (C-X-C motif) ligand 12 is associated with gallbladder carcinoma progression and is a novel independent poor prognostic factor[J].Clin Cancer Res, 2012, 18(12):3270-3280.
14.	Yao X, Zhou L, Han S, et al.High expression of CXCR4 and CXCR7 predicts poor survival in gallbladder cancer[J].J Int Med Res, 2011, 39(4):1253-1264.
15.	Sun X, Cheng G, Hao M, et al.CXCL12/CXCR4/CXCR7 chemokine axis and cancer progression[J].Cancer Metastasis Rev, 2010, 29(4):709-722.
16.	Ping YF, Yao XH, Jiang JY, et al.The chemokine CXCL12 and its receptor CXCR4 promote glioma stem cell-mediated VEGF production and tumour angiogenesis via PI3K/AKT signalling[J]. J Pathol, 2011, 224(3):344-354.
17.	姜海廣, 陸瑞祺, 姜波健, 等.基質細胞源性因子-1α/CXC趨化因子受體-4軸經PI3K/Akt通路對胃癌細胞CD133表達的調控作用[J].中華實驗外科雜志, 2012, 29(3):378-380.
18.	Zhang SS, Han ZP, Jing YY, et al.C D133⁺CXCR4⁺colon cancer cells exhibit metastatic potential and predict poor prognosis of patients[J].BMC Med, 2012, 10:85.

1. Boutros C, Gary M, Baldwin K, et al.Gallbladder cancer:past, present and an uncertain future[J].Surg Oncol, 2012, 21(4):183-191.
2. Sugihara E, Saya H.Complexity of cancer stem cells[J].Int J Cancer, 2013, 132(6):1249-1259.
3. 俞遠林, 姜波健, 俞繼衛, 等.免疫磁珠法分離膽囊癌CD133陽性細胞及生物學行為的研究[J].中華實驗外科雜志, 2012, 30(8):1652-1655.
4. Domanska UM, Timmer-Bosscha H, Nagengast WB, et al.CXCR4 inhibition with AMD3100 sensitizes prostate cancer to docetaxel chemotherapy[J].Neoplasia, 2012, 14(8):709-718.
5. 陸瑞祺, 吳巨鋼, 周國才, 等.胃癌C D133陽性細胞亞群腫瘤起始細胞樣特性的檢測[J].中華胃腸外科雜志, 2012, 15(2):174-179.
6. Ricci-Vitiani L, Lombardi DG, Pilozzi E, et al.Identification and expansion of human colon-cancer-initiating cells[J].Nature, 2007, 445(7123):111-115.
7. Galli R, Binda E, Orfanelli U, et al.Isolation and characterization of tumorigenic, stem-like neural precursors from human glioblastoma[J].Cancer Res, 2004, 64(19):7011-7021.
8. Bao S, Wu Q, McLendon RE, et al.Glioma stem cells promote radioresistance by preferential activation of the DNA damage res-ponse[J].Nature, 2006, 444(7120):756-760.
9. Yin S, Li J, Hu C, et al.C D133 positive hepatocellular carcinoma cells possess high capacity for tumorigenicity[J].Int J Cancer, 2007, 120(7):1444-1450.
10. Müller A, Homey B, Soto H, et al.Involvement of chemokine receptors in breast cancer metastasis[J].Nature, 2001, 410(6824):50-56.
11. Saur D, Seidler B, Schneider G, et al.CXCR4 expression incr-eases liver and lung metastasis in a mouse model of pancreatic cancer[J].Gastroenterology, 2005, 129(4):1237-1250.
12. Schimanski CC, Schwald S, Simiantonaki N, et al.Effect of chemokine receptors CXCR4 and CCR7 on the metastatic behaviorof human colorectal cancer[J].Clin Cancer Res, 2005, 11(5):1743-1750.
13. Lee HJ, Lee K, Lee DG, et al.Chemokine (C-X-C motif) ligand 12 is associated with gallbladder carcinoma progression and is a novel independent poor prognostic factor[J].Clin Cancer Res, 2012, 18(12):3270-3280.
14. Yao X, Zhou L, Han S, et al.High expression of CXCR4 and CXCR7 predicts poor survival in gallbladder cancer[J].J Int Med Res, 2011, 39(4):1253-1264.
15. Sun X, Cheng G, Hao M, et al.CXCL12/CXCR4/CXCR7 chemokine axis and cancer progression[J].Cancer Metastasis Rev, 2010, 29(4):709-722.
16. Ping YF, Yao XH, Jiang JY, et al.The chemokine CXCL12 and its receptor CXCR4 promote glioma stem cell-mediated VEGF production and tumour angiogenesis via PI3K/AKT signalling[J]. J Pathol, 2011, 224(3):344-354.
17. 姜海廣, 陸瑞祺, 姜波健, 等.基質細胞源性因子-1α/CXC趨化因子受體-4軸經PI3K/Akt通路對胃癌細胞CD133表達的調控作用[J].中華實驗外科雜志, 2012, 29(3):378-380.
18. Zhang SS, Han ZP, Jing YY, et al.C D133⁺CXCR4⁺colon cancer cells exhibit metastatic potential and predict poor prognosis of patients[J].BMC Med, 2012, 10:85.

Previous Article
Anticoagulation for Patients with Large Left Atrium after Mitral Valve Replacement
Next Article
Anticoagulation for Patients with Large Left Atrium after Mitral Valve Replacement

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study on Mechanism of Invasion of CD133 Positive Population in Gallbladder Cancer

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content