Research advances on extracorporeal membrane oxygenation in rat model_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

WANG Tianlong , YAN Weidong ,  JI Bingyang

Department of Cardiopulmonary Bypass, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, P. R. China;

Corresponding?author：

JI Bingyang, Email: jibingyang@fuwai.com

Keywords：

Extracorporeal membrane oxygenation; animal experiment; rats; review

DOI：

10.7507/1007-4848.202305012

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Extracorporeal membrane oxygenation (ECMO) is a critical life support technique for patients with severe cardiopulmonary failure. Establishing a stable ECMO animal model is essential to further investigate the effects of ECMO on the body and provide assistance for optimizing ECMO management strategies and preventing complications in clinical practice. In recent years, rats have been widely used to establish ECMO models due to their low cost and good reproducibility. Therefore, this article provided a comprehensive review of literature on the ECMO rat model, including equipment and experimental management strategies. It offers a theoretical foundation for the development of a stable and mature ECMO rat model in the future.

Citation： WANG Tianlong, YAN Weidong, JI Bingyang. Research advances on extracorporeal membrane oxygenation in rat model. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2024, 31(6): 915-921. doi: 10.7507/1007-4848.202305012 Copy

1.	Li ZJ, Zhang DF, Zhang WH. Analysis of nosocomial infection and risk factors in patients with ECMO treatment. Infect Drug Resist, 2021, 14: 2403-2410.
2.	Mahmoud AA, Avedissian SN, Al-Qamari A, et al. Pharmacokinetic assessment of pre- and post-oxygenator vancomycin concentrations in extracorporeal membrane oxygenation: A prospective observational study. Clin Pharmacokinet, 2020, 59(12): 1575-1587.
3.	Pequignot B, Lescroart M, Orlowski S, et al. Methylene blue reduces fluid loading and norepinephrine requirements for post-resuscitation syndrome in a pig model of refractory cardiac arrest resuscitated with veno-arterial ECMO. J Clin Med, 2022, 11(9): 2515.
4.	Qi J, Gao S, Liu G, et al. An ovine model of awake veno-arterial extracorporeal membrane oxygenation. Front Vet Sci, 2021, 8: 809487.
5.	Tweddell JS, Kharnaf M, Zafar F, et al. Targeting the contact system in a rabbit model of extracorporeal membrane oxygenation. Blood Adv, 2023, 7(8): 1404-1417.
6.	薛平菲, 于潔, 黑飛龍. 大鼠靜脈-動脈體外膜氧合模型研究進展. 中國體外循環雜志, 2019, 17(5): 318-320.
7.	陳原森, 劉子泉, 王海旺, 等. 大鼠體外膜肺氧合設備的研制與模型應用. 中華災害救援醫學, 2021, 9(8): 1171-1175.
8.	Juhl L, Petersen KA, Larsen EH, et al. The in vivo effect of adrenomedullin on rat dural and pial arteries. Eur J Pharmacol, 2006, 538(1-3): 101-107.
9.	Janata A, Drabek T, Magnet IA, et al. Extracorporeal versus conventional cardiopulmonary resuscitation after ventricular fibrillation cardiac arrest in rats: A feasibility trial. Crit Care Med, 2013, 41(9): e211-e222.
10.	Chang RW, Luo CM, Yu HY, et al. Investigation of the pathophysiology of cardiopulmonary bypass using rodent extracorporeal life support model. BMC Cardiovasc Disord, 2017, 17(1): 123.
11.	Ahmed N, Laghari AH, AlBkhoor B, et al. Fingolimod plays role in attenuation of myocardial injury related to experimental model of cardiac arrest and extracorporeal life support resuscitation. Int J Mol Sci, 2019, 20(24): 6237.
12.	于潔, 薛平菲, 陳書弘, 等. 大鼠體外心肺復蘇模型的建立與評價. 中國體外循環雜志, 2020, 18(1): 44-47.
13.	薛平菲, 于潔, 秦春妮, 等. 無血預充大鼠體外心肺復蘇模型的建立及支持效果評價. 中國分子心臟病學雜志, 2021, 21(1): 3748-3752.
14.	陳燕樺, 張炳東, 梁東科, 等. 體外膜肺氧合對急性肺損傷治療的實驗研究. 中國現代醫學雜志, 2011, 21(19): 2215-2218.
15.	Ali AA, Downey P, Singh G, et al. Rat model of veno-arterial extracorporeal membrane oxygenation. J Transl Med, 2014, 12: 37.
16.	Fujii Y, Tatsumi E, Nakamura F, et al. PaO₂ greater than 300 mm Hg promotes an inflammatory response during extracorporeal circulation in a rat extracorporeal membrane oxygenation model. J Thorac Dis, 2020, 12(3): 749-757.
17.	Edinger F, Schneck E, Schulte C, et al. Comparison of the effect of membrane sizes and fibre arrangements of two membrane oxygenators on the inflammatory response, oxygenation and decarboxylation in a rat model of extracorporeal membrane oxygenation. BMC Cardiovasc Disord, 2020, 20(1): 294.
18.	Edinger F, Schmitt C, Koch C, et al. Application of alpha1-antitrypsin in a rat model of veno-arterial extracorporeal membrane oxygenation. Sci Rep, 2021, 11(1): 15849.
19.	Fujii Y, Abe T, Ikegami K. Diabetic pathophysiology enhances inflammation during extracorporeal membrane oxygenation in a rat model. Membranes (Basel), 2021, 11(4): 283.
20.	Umei N, Lai A, Miller J, et al. Establishment and evaluation of a rat model of extracorporeal membrane oxygenation (ECMO) thrombosis using a 3D-printed mock-oxygenator. J Transl Med, 2021, 19(1): 179.
21.	Xing Y, Cheng D, Shi C, et al. The protective role of YTHDF1-knock down macrophages on the immune paralysis of severe sepsis rats with ECMO. Microvasc Res, 2021, 137: 104178.
22.	Cheng X, Zhang R, Wei S, et al. Dexamethasone alleviates myocardial injury in a rat model of acute myocardial infarction supported by venoarterial extracorporeal membrane oxygenation. Front Public Health, 2022, 10: 900751.
23.	Edinger F, Schneck E, Schulte C, et al. Impact of the inspiratory oxygen fraction on the cardiac output during jugulo-femoral venoarterial extracorporeal membrane oxygenation in the rat. BMC Cardiovasc Disord, 2022, 22(1): 174.
24.	Wei S, Cheng X, Li J, et al. Venoarterial extracorporeal membrane oxygenation improves survival in a rat model of acute myocardial infarction. Artif Organs, 2023, 47(7): 1163-1173.
25.	蔡黎明, 張巧妮, 李勇男, 等. 無血預充大鼠外周靜脈-動脈體外膜氧合模型建立的初步探索. 中國體外循環雜志, 2019, 17(6): 360-363.
26.	Zazzeron L, Fischbach A, Franco W, et al. Phototherapy and extracorporeal membrane oxygenation facilitate removal of carbon monoxide in rats. Sci Transl Med, 2019, 11(513): eaau4217.
27.	Fujii Y, Sumikura H, Nagahama D. Establishment of a novel miniature veno-venous extracorporeal membrane oxygenation model in the rat. Artif Organs, 2021, 45(1): 63-67.
28.	Li Y, Huang J, Zhang R, et al. Establishment of a venovenous extracorporeal membrane oxygenation in a rat model of acute respiratory distress syndrome. Perfusion, 2023, 38(1): 85-91.
29.	Zhang R, Zhai K, Huang J, et al. Sevoflurane alleviates lung injury and inflammatory response compared with propofol in a rat model of VV ECMO. Perfusion, 2024, 39(1):142-150.
30.	Huang J, Zhang R, Zhai K, et al. Venovenous extracorporeal membrane oxygenation promotes alveolar epithelial recovery by activating Hippo/YAP signaling after lung injury. J Heart Lung Transplant, 2022, 41(10): 1391-1400.
31.	紀振華, 武婷. 離心泵體外循環的應用進展. 天津醫藥, 2019, 47(10): 1117-1120.
32.	Etchill EW, Dante SA, Garcia AV. Extracorporeal membrane oxygenation in the pediatric population: Who should go on, and who should not. Curr Opin Pediatr, 2020, 32(3): 416-423.
33.	Wang H, Chen F, Li J, et al. Vaspin antagonizes high fat-induced bone loss in rats and promotes osteoblastic differentiation in primary rat osteoblasts through Smad-Runx2 signaling pathway. Nutr Metab (Lond), 2020, 17: 9.
34.	Chlebowski MM, Baltagi S, Carlson M, et al. Clinical controversies in anticoagulation monitoring and antithrombin supplementation for ECMO. Crit Care, 2020, 24(1): 19.
35.	Djordjevic I, Liakopoulos O, Elskamp M, et al. Concomitant intra-aortic balloon pumping significantly reduces left ventricular pressure during central veno-arterial extracorporeal membrane oxygenation: Results from a large animal model. Life (Basel), 2022, 12(11): 1859.

1. Li ZJ, Zhang DF, Zhang WH. Analysis of nosocomial infection and risk factors in patients with ECMO treatment. Infect Drug Resist, 2021, 14: 2403-2410.
2. Mahmoud AA, Avedissian SN, Al-Qamari A, et al. Pharmacokinetic assessment of pre- and post-oxygenator vancomycin concentrations in extracorporeal membrane oxygenation: A prospective observational study. Clin Pharmacokinet, 2020, 59(12): 1575-1587.
3. Pequignot B, Lescroart M, Orlowski S, et al. Methylene blue reduces fluid loading and norepinephrine requirements for post-resuscitation syndrome in a pig model of refractory cardiac arrest resuscitated with veno-arterial ECMO. J Clin Med, 2022, 11(9): 2515.
4. Qi J, Gao S, Liu G, et al. An ovine model of awake veno-arterial extracorporeal membrane oxygenation. Front Vet Sci, 2021, 8: 809487.
5. Tweddell JS, Kharnaf M, Zafar F, et al. Targeting the contact system in a rabbit model of extracorporeal membrane oxygenation. Blood Adv, 2023, 7(8): 1404-1417.
6. 薛平菲, 于潔, 黑飛龍. 大鼠靜脈-動脈體外膜氧合模型研究進展. 中國體外循環雜志, 2019, 17(5): 318-320.
7. 陳原森, 劉子泉, 王海旺, 等. 大鼠體外膜肺氧合設備的研制與模型應用. 中華災害救援醫學, 2021, 9(8): 1171-1175.
8. Juhl L, Petersen KA, Larsen EH, et al. The in vivo effect of adrenomedullin on rat dural and pial arteries. Eur J Pharmacol, 2006, 538(1-3): 101-107.
9. Janata A, Drabek T, Magnet IA, et al. Extracorporeal versus conventional cardiopulmonary resuscitation after ventricular fibrillation cardiac arrest in rats: A feasibility trial. Crit Care Med, 2013, 41(9): e211-e222.
10. Chang RW, Luo CM, Yu HY, et al. Investigation of the pathophysiology of cardiopulmonary bypass using rodent extracorporeal life support model. BMC Cardiovasc Disord, 2017, 17(1): 123.
11. Ahmed N, Laghari AH, AlBkhoor B, et al. Fingolimod plays role in attenuation of myocardial injury related to experimental model of cardiac arrest and extracorporeal life support resuscitation. Int J Mol Sci, 2019, 20(24): 6237.
12. 于潔, 薛平菲, 陳書弘, 等. 大鼠體外心肺復蘇模型的建立與評價. 中國體外循環雜志, 2020, 18(1): 44-47.
13. 薛平菲, 于潔, 秦春妮, 等. 無血預充大鼠體外心肺復蘇模型的建立及支持效果評價. 中國分子心臟病學雜志, 2021, 21(1): 3748-3752.
14. 陳燕樺, 張炳東, 梁東科, 等. 體外膜肺氧合對急性肺損傷治療的實驗研究. 中國現代醫學雜志, 2011, 21(19): 2215-2218.
15. Ali AA, Downey P, Singh G, et al. Rat model of veno-arterial extracorporeal membrane oxygenation. J Transl Med, 2014, 12: 37.
16. Fujii Y, Tatsumi E, Nakamura F, et al. PaO₂ greater than 300 mm Hg promotes an inflammatory response during extracorporeal circulation in a rat extracorporeal membrane oxygenation model. J Thorac Dis, 2020, 12(3): 749-757.
17. Edinger F, Schneck E, Schulte C, et al. Comparison of the effect of membrane sizes and fibre arrangements of two membrane oxygenators on the inflammatory response, oxygenation and decarboxylation in a rat model of extracorporeal membrane oxygenation. BMC Cardiovasc Disord, 2020, 20(1): 294.
18. Edinger F, Schmitt C, Koch C, et al. Application of alpha1-antitrypsin in a rat model of veno-arterial extracorporeal membrane oxygenation. Sci Rep, 2021, 11(1): 15849.
19. Fujii Y, Abe T, Ikegami K. Diabetic pathophysiology enhances inflammation during extracorporeal membrane oxygenation in a rat model. Membranes (Basel), 2021, 11(4): 283.
20. Umei N, Lai A, Miller J, et al. Establishment and evaluation of a rat model of extracorporeal membrane oxygenation (ECMO) thrombosis using a 3D-printed mock-oxygenator. J Transl Med, 2021, 19(1): 179.
21. Xing Y, Cheng D, Shi C, et al. The protective role of YTHDF1-knock down macrophages on the immune paralysis of severe sepsis rats with ECMO. Microvasc Res, 2021, 137: 104178.
22. Cheng X, Zhang R, Wei S, et al. Dexamethasone alleviates myocardial injury in a rat model of acute myocardial infarction supported by venoarterial extracorporeal membrane oxygenation. Front Public Health, 2022, 10: 900751.
23. Edinger F, Schneck E, Schulte C, et al. Impact of the inspiratory oxygen fraction on the cardiac output during jugulo-femoral venoarterial extracorporeal membrane oxygenation in the rat. BMC Cardiovasc Disord, 2022, 22(1): 174.
24. Wei S, Cheng X, Li J, et al. Venoarterial extracorporeal membrane oxygenation improves survival in a rat model of acute myocardial infarction. Artif Organs, 2023, 47(7): 1163-1173.
25. 蔡黎明, 張巧妮, 李勇男, 等. 無血預充大鼠外周靜脈-動脈體外膜氧合模型建立的初步探索. 中國體外循環雜志, 2019, 17(6): 360-363.
26. Zazzeron L, Fischbach A, Franco W, et al. Phototherapy and extracorporeal membrane oxygenation facilitate removal of carbon monoxide in rats. Sci Transl Med, 2019, 11(513): eaau4217.
27. Fujii Y, Sumikura H, Nagahama D. Establishment of a novel miniature veno-venous extracorporeal membrane oxygenation model in the rat. Artif Organs, 2021, 45(1): 63-67.
28. Li Y, Huang J, Zhang R, et al. Establishment of a venovenous extracorporeal membrane oxygenation in a rat model of acute respiratory distress syndrome. Perfusion, 2023, 38(1): 85-91.
29. Zhang R, Zhai K, Huang J, et al. Sevoflurane alleviates lung injury and inflammatory response compared with propofol in a rat model of VV ECMO. Perfusion, 2024, 39(1):142-150.
30. Huang J, Zhang R, Zhai K, et al. Venovenous extracorporeal membrane oxygenation promotes alveolar epithelial recovery by activating Hippo/YAP signaling after lung injury. J Heart Lung Transplant, 2022, 41(10): 1391-1400.
31. 紀振華, 武婷. 離心泵體外循環的應用進展. 天津醫藥, 2019, 47(10): 1117-1120.
32. Etchill EW, Dante SA, Garcia AV. Extracorporeal membrane oxygenation in the pediatric population: Who should go on, and who should not. Curr Opin Pediatr, 2020, 32(3): 416-423.
33. Wang H, Chen F, Li J, et al. Vaspin antagonizes high fat-induced bone loss in rats and promotes osteoblastic differentiation in primary rat osteoblasts through Smad-Runx2 signaling pathway. Nutr Metab (Lond), 2020, 17: 9.
34. Chlebowski MM, Baltagi S, Carlson M, et al. Clinical controversies in anticoagulation monitoring and antithrombin supplementation for ECMO. Crit Care, 2020, 24(1): 19.
35. Djordjevic I, Liakopoulos O, Elskamp M, et al. Concomitant intra-aortic balloon pumping significantly reduces left ventricular pressure during central veno-arterial extracorporeal membrane oxygenation: Results from a large animal model. Life (Basel), 2022, 12(11): 1859.

Previous Article
Application and ethical exploration of ChatGPT in medical clinical practice
Next Article
Current epidemiology and trend of hypertension in Chinese adult

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Research advances on extracorporeal membrane oxygenation in rat model

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content