Establishment of patent ductus arteriosus model in Bama miniature pigs using autogenous jugular vein_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

LI Jian ^1,2,3,4 , LI Wenchao ⁵ , ZHANG Fengwen ^1,2,3,4 , ZHUANG Donglin ^1,2,3,4 , WEI Peijian ^1,2,3,4 , LI Hang ^1,2,3,4 , ZHANG Min ⁶ ,  OUYANG Wenbin ^1,2,3,4 ,  PAN Xiangbin ^1,2,3,4

1. Department of Structural Heart Disease, National Center for Cardiovascular Disease, Fuwai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100037, P. R. China;
2. National Health Commission Key Laboratory of Cardiovascular Regeneration Medicine, Beijing, 100037, P. R. China;
3. National Clinical Research Center for Cardiovascular Diseases, Fuwai Hospital, Chinese Academy of Medical Sciences, Beijing, 100037, P. R. China;
4. Key Laboratory of Innovative Cardiovascular Devices, Chinese Academy of Medical Sciences, Beijing, 100037, P. R. China;
5. Children’s Heart Center, Huazhong Fuwai Hospital, Heart Center, Henan Provincial People’s Hospital, Zhengzhou University People’s Hospital, Zhengzhou, 450000, P. R. China;
6. National Center for Cardiovascular Disease, Animal Experimental Center of Fuwai Hospital, Chinese Academy of Medical Sciences, Beijing Key Laboratory of Pre-clinical Research and Evaluation for Cardiovascular Implant Materials, Beijing, 102300, P. R. China;

Corresponding?author：

OUYANG Wenbin, Email: droywb31@163.com; PAN Xiangbin, Email: panxiangbin@fuwaihospital.org

Keywords：

Congenital heart disease; patent ductus arteriosus; interventional closure; pulmonary hypertension; animal models

DOI：

10.7507/1007-4848.202301037

Video：

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Objective To explore the method and feasibility of establishing patent ductus arteriosus (PDA) model in Bama miniature pig by using autologous jugular vein, and to provide a large animal model for the development of PDA occluder and the study of pulmonary hypertension associated with congenital heart disease. Methods Five male Bama miniature pigs weighing about 45 kg were selected to gain the PDA model of the autogenous jugular vein, which was fixed by glutaraldehyde and anastomosed between the ascending aorta and the main pulmonary artery. The patency of PDA was confirmed by echocardiography and angiocardiography immediately and one week after the operation. Two animals were selected to undergo transcatheter closure of PDA via femoral vein 1 week after the operation, and the rest were euthanized to obtain PDA and lung tissue for pathological examination. Results The PDA model was successfully established in all five animals with a success rate of 100.0%. Immediately and 1 week after the operation, echocardiography and angiography showed that PDA blood flow was unobstructed, and hematoxylin-eosin staining showed that PDA endothelialization was good. One week after the operation, two animals were successfully treated with transcatheter femoral vein occlusion. The pathological examination of lung tissue showed thickening of the intima and muscular layer of pulmonary arterioles, thickening of pulmonary interstitium and infiltration of neutrophils. Conclusion It is safe and feasible to establish a large animal model of PDA by using autogenous jugular vein anastomosis between the ascending aorta and the main pulmonary artery. The model can be used for the development of PDA interventional occlusive devices and the pathophysiological study of congenital heart disease-related pulmonary hypertension.

Citation： LI Jian, LI Wenchao, ZHANG Fengwen, ZHUANG Donglin, WEI Peijian, LI Hang, ZHANG Min, OUYANG Wenbin, PAN Xiangbin. Establishment of patent ductus arteriosus model in Bama miniature pigs using autogenous jugular vein. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2024, 31(12): 1832-1837. doi: 10.7507/1007-4848.202301037 Copy

1.	Pan F, Li J, Lou H, et al. Geographical and socioeconomic factors influence the birth prevalence of congenital heart disease: A population-based cross-sectional study in Eastern China. Curr Probl Cardiol, 2022, 47(11): 101341.
2.	van der Linde D, Konings EE, Slager MA, et al. Birth prevalence of congenital heart disease worldwide: A systematic review and meta-analysis. J Am Coll Cardiol, 2011, 58(21): 2241-2247.
3.	Sehgal A, McNamara PJ. International perspective on management of a patent ductus arteriosus: Lessons learned. Semin Fetal Neonatal Med, 2018, 23(4): 278-284.
4.	Ku L, Cheng Y, Ma X. Infectious endarteritis associated with patent ductus arteriosus and vegetation: A challenging diagnosis and treatment. Eur Heart J, 2022, 43(23): 2251.
5.	國家衛生健康委員會國家結構性心臟病介入質量控制中心, 國家心血管病中心結構性心臟病介入質量控制中心, 中華醫學會心血管病學分會先心病經皮介入治療指南工作組, 等. 常見先天性心臟病經皮介入治療指南(2021版). 中華醫學雜志, 2021, 101(38): 3054-3076.National Health Commission National Structural Heart Disease Intervention Quality Control Center, National Cardiovascular Disease Center Structural Heart Disease Intervention Quality Control Center, Chinese Medical Association Cardiovascular Disease Branch Congenital Heart Disease Percutaneous Intervention Treatment Guidelines Working Group, et al. Common congenital heart disease percutaneous intervention treatment guidelines (2021 edition). Nat Med J China, 2021, 101(38): 3054-3076.
6.	Baumgartner H, De Backer J, Babu-Narayan SV, et al. 2020 ESC guidelines for the management of adult congenital heart disease. Eur Heart J, 2021, 42(6): 563-645.
7.	Yarboro MT, Gopal SH, Su RL, et al. Mouse models of patent ductus arteriosus (PDA) and their relevance for human PDA. Dev Dyn, 2022, 251(3): 424-443.
8.	袁運杰, 秦永文, 湯敬東, 等. 應用自體血管制作的犬動脈導管未閉模型. 介入放射學雜志, 2006, 15(9): 552-554.Yuan YJ, Qin YW, Tang JD, et al. The preparation of patent ductus arteriosus model in dog by using self-vessel. J Interv Radiol, 2006, 15(9): 552-554.
9.	李俊杰, 曾國洪, 張智偉, 等. 一種國產動脈導管未閉堵閉器的動物試驗評價. 中華心血管病雜志, 2001, 29(10): 622-625.Li JJ, Zeng GH, Zhang ZW, et al. Experimental evaluation of a homemade patent ductus arteriosus closure device. Chin J Cardiol, 2001, 29(10): 622-625.
10.	Pozza CH, Gomes MR, Qian Z, et al. Transcatheter occlusion of patent ductus arteriosus using a newly developed self-expanding device. Evaluation in a canine model. Invest Radiol, 1995, 30(2): 104-109.
11.	Sharafuddin MJ, Gu X, Titus JL, et al. Experimental evaluation of a new self-expanding patent ductus arteriosus occluder in a canine model. J Vasc Interv Radiol, 1996, 7(6): 877-887.
12.	Grabitz RG, Freudenthal F, Sigler M, et al. Double-helix coil for occlusion of large patent ductus arteriosus: Evaluation in a chronic lamb model. J Am Coll Cardiol, 1998, 31(3): 677-683.
13.	Sigler M, Handt S, Seghaye MC, et al. Evaluation of in vivo biocompatibility of different devices for interventional closure of the patent ductus arteriosus in an animal model. Heart, 2000, 83(5): 570-573.
14.	Jiang HB, Bai Y, Zong GJ, et al. Pan-nitinol occluder and special delivery device for closure of patent ductus arteriosus: A canine-model feasibility study. Tex Heart Inst J, 2013, 40(1): 30-33.
15.	Chen L, Hu S, Luo Z, et al. First-in-human experience with a novel fully bioabsorbable occluder for ventricular septal defect. JACC Cardiovasc Interv, 2020, 13(9): 1139-1141.
16.	張鳳文, 孫毅, 謝涌泉, 等. 完全可降解封堵器治療膜周部室間隔缺損兩例. 中國胸心血管外科臨床雜志, 2018, 25(7): 636-638.Zhang FW, Sun Y, Xie YQ, et al. Perimembranous ventricular septal defect treated with a completely biodegradable occluder: Two cases report. Chin J Clin Thorac Cardiovasc Surg, 2018, 25(7): 636-638.
17.	Kong P, Zhao G, Zhang Z, et al. Novel panna guide wire facilitates percutaneous and nonfluoroscopic procedure for atrial septal defect closure: A randomized controlled trial. Circ Cardiovasc Interv, 2020, 13(9): e009281.
18.	Pan XB, Ou-Yang WB, Pang KJ, et al. Percutaneous closure of atrial septal defects under transthoracic echocardiography guidance without fluoroscopy or intubation in children. J Interv Cardiol, 2015, 28(4): 390-395.
19.	Wen B, Peng R, Kong P, et al. Neuroblastoma suppressor of tumorigenicity 1 mediates endothelial-to-mesenchymal transition in pulmonary arterial hypertension related to congenital heart disease. Am J Respir Cell Mol Biol, 2022, 67(6): 666-679.
20.	Duport O, Le Rolle V, Guerrero G, et al. Sensitivity analysis of a cardio-respiratory model in preterm newborns for the study of patent ductus arteriosus. Annu Int Conf IEEE Eng Med Biol Soc, 2021, 2021: 4420-4423.

1. Pan F, Li J, Lou H, et al. Geographical and socioeconomic factors influence the birth prevalence of congenital heart disease: A population-based cross-sectional study in Eastern China. Curr Probl Cardiol, 2022, 47(11): 101341.
2. van der Linde D, Konings EE, Slager MA, et al. Birth prevalence of congenital heart disease worldwide: A systematic review and meta-analysis. J Am Coll Cardiol, 2011, 58(21): 2241-2247.
3. Sehgal A, McNamara PJ. International perspective on management of a patent ductus arteriosus: Lessons learned. Semin Fetal Neonatal Med, 2018, 23(4): 278-284.
4. Ku L, Cheng Y, Ma X. Infectious endarteritis associated with patent ductus arteriosus and vegetation: A challenging diagnosis and treatment. Eur Heart J, 2022, 43(23): 2251.
5. 國家衛生健康委員會國家結構性心臟病介入質量控制中心, 國家心血管病中心結構性心臟病介入質量控制中心, 中華醫學會心血管病學分會先心病經皮介入治療指南工作組, 等. 常見先天性心臟病經皮介入治療指南(2021版). 中華醫學雜志, 2021, 101(38): 3054-3076.National Health Commission National Structural Heart Disease Intervention Quality Control Center, National Cardiovascular Disease Center Structural Heart Disease Intervention Quality Control Center, Chinese Medical Association Cardiovascular Disease Branch Congenital Heart Disease Percutaneous Intervention Treatment Guidelines Working Group, et al. Common congenital heart disease percutaneous intervention treatment guidelines (2021 edition). Nat Med J China, 2021, 101(38): 3054-3076.
6. Baumgartner H, De Backer J, Babu-Narayan SV, et al. 2020 ESC guidelines for the management of adult congenital heart disease. Eur Heart J, 2021, 42(6): 563-645.
7. Yarboro MT, Gopal SH, Su RL, et al. Mouse models of patent ductus arteriosus (PDA) and their relevance for human PDA. Dev Dyn, 2022, 251(3): 424-443.
8. 袁運杰, 秦永文, 湯敬東, 等. 應用自體血管制作的犬動脈導管未閉模型. 介入放射學雜志, 2006, 15(9): 552-554.Yuan YJ, Qin YW, Tang JD, et al. The preparation of patent ductus arteriosus model in dog by using self-vessel. J Interv Radiol, 2006, 15(9): 552-554.
9. 李俊杰, 曾國洪, 張智偉, 等. 一種國產動脈導管未閉堵閉器的動物試驗評價. 中華心血管病雜志, 2001, 29(10): 622-625.Li JJ, Zeng GH, Zhang ZW, et al. Experimental evaluation of a homemade patent ductus arteriosus closure device. Chin J Cardiol, 2001, 29(10): 622-625.
10. Pozza CH, Gomes MR, Qian Z, et al. Transcatheter occlusion of patent ductus arteriosus using a newly developed self-expanding device. Evaluation in a canine model. Invest Radiol, 1995, 30(2): 104-109.
11. Sharafuddin MJ, Gu X, Titus JL, et al. Experimental evaluation of a new self-expanding patent ductus arteriosus occluder in a canine model. J Vasc Interv Radiol, 1996, 7(6): 877-887.
12. Grabitz RG, Freudenthal F, Sigler M, et al. Double-helix coil for occlusion of large patent ductus arteriosus: Evaluation in a chronic lamb model. J Am Coll Cardiol, 1998, 31(3): 677-683.
13. Sigler M, Handt S, Seghaye MC, et al. Evaluation of in vivo biocompatibility of different devices for interventional closure of the patent ductus arteriosus in an animal model. Heart, 2000, 83(5): 570-573.
14. Jiang HB, Bai Y, Zong GJ, et al. Pan-nitinol occluder and special delivery device for closure of patent ductus arteriosus: A canine-model feasibility study. Tex Heart Inst J, 2013, 40(1): 30-33.
15. Chen L, Hu S, Luo Z, et al. First-in-human experience with a novel fully bioabsorbable occluder for ventricular septal defect. JACC Cardiovasc Interv, 2020, 13(9): 1139-1141.
16. 張鳳文, 孫毅, 謝涌泉, 等. 完全可降解封堵器治療膜周部室間隔缺損兩例. 中國胸心血管外科臨床雜志, 2018, 25(7): 636-638.Zhang FW, Sun Y, Xie YQ, et al. Perimembranous ventricular septal defect treated with a completely biodegradable occluder: Two cases report. Chin J Clin Thorac Cardiovasc Surg, 2018, 25(7): 636-638.
17. Kong P, Zhao G, Zhang Z, et al. Novel panna guide wire facilitates percutaneous and nonfluoroscopic procedure for atrial septal defect closure: A randomized controlled trial. Circ Cardiovasc Interv, 2020, 13(9): e009281.
18. Pan XB, Ou-Yang WB, Pang KJ, et al. Percutaneous closure of atrial septal defects under transthoracic echocardiography guidance without fluoroscopy or intubation in children. J Interv Cardiol, 2015, 28(4): 390-395.
19. Wen B, Peng R, Kong P, et al. Neuroblastoma suppressor of tumorigenicity 1 mediates endothelial-to-mesenchymal transition in pulmonary arterial hypertension related to congenital heart disease. Am J Respir Cell Mol Biol, 2022, 67(6): 666-679.
20. Duport O, Le Rolle V, Guerrero G, et al. Sensitivity analysis of a cardio-respiratory model in preterm newborns for the study of patent ductus arteriosus. Annu Int Conf IEEE Eng Med Biol Soc, 2021, 2021: 4420-4423.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Establishment of patent ductus arteriosus model in Bama miniature pigs using autogenous jugular vein

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