Lienal polypeptide injection attenuates lipopolysaccharide-induced acute lung injury in rats by metastasis-associated protein 1_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

GE Peng ¹ , LI Hanjie ¹ , CHEN Xin ¹ , JING Ruijun ¹ , YAO Yuejuan ¹ , LI Jianzhong ² , ZHANG Weidong ³ ,  YANG Bo ³

1. Department of Cardio-Thoracic Surgery, Second Affiliated Hospital, Xi'an Medical College, Xi'an, 710038, P.R.China;
2. Department of Thoracic Surgery, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, P.R.China;
3. Department of Thoracic Surgery, Tianjin First Central Hospital of Nankai University, Tianjin, 300192, P.R.China;

Corresponding?author：

YANG Bo, Email: yangbochest@nankai.edu.cn

Keywords：

Lienal polypeptide injection; lipopolysaccharide; acute lung injury; metastasis-associated protein 1

DOI：

10.7507/1007-4848.201803045

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Objective To analyze the role of lienal polypeptide injection in acute lung injury induced by lipopolysaccharide (LPS) in rats. Methods Eighty male SD rats were randomly allocated into 4 groups: a LPS group, a control group, a lienal polypeptide group and a LPS+ lienal polypeptide group (20 rats in each group). Lienal polypeptide or normal saline was given with an intramuscular injection 30 min after an intraperitoneal injection of LPS (5 mg/kg). The severity of pulmonary injury was evaluated 4 h after LPS challenge by enzyme-linked immunosorbent assay (ELISA), wet-to-dry weight ratio, hematoxylin and eosin (HE) staining, TUNEL and Western blotting. Results Lienal polypeptide injection treatment significantly attenuated LPS-induced pulmonary histopathologic changes, alveolar hemorrhage, and neutrophil infiltration. Moreover lienal polypeptide injection significantly suppressed LPS-induced activation of metastasis-associated protein-1 (MTA1). Conclusion Lienal polypeptide injection is demonstrated to protect rats from LPS-induced acute lung injury by the expression of MTA1.

Citation： GE Peng, LI Hanjie, CHEN Xin, JING Ruijun, YAO Yuejuan, LI Jianzhong, ZHANG Weidong, YANG Bo. Lienal polypeptide injection attenuates lipopolysaccharide-induced acute lung injury in rats by metastasis-associated protein 1. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2019, 26(3): 264-268. doi: 10.7507/1007-4848.201803045 Copy

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2.	Zhou J, Niu G, Pei Y, et al. The effect and clinical efficacy of lienal polypeptide injection combined with FOLFOX chemotherapy regimen in colon cancer patients. Oncol Lett, 2016, 12(5): 3191-3194.
3.	Huang XE, Wang L, Ji ZQ, et al. Safety of lienal polypeptide injection combined with chemotherapy in treating patients with advanced cancer. Asian Pac Cancer Prev, 2015, 16(17): 7837-7841.
4.	蘇俊孟, 郭暉, 夏飛, 等. 脾多肽對急性肺損傷大鼠 TLR9 信號相關因子的影響. 貴州醫科大學學報, 2017, 42(3): 296-299.
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6.	Yang B, Ni YF, Wang WC, et al. Melatonin attenuates intestinal ischemia--reperfusion-induced lung injury in rats by upregulating N-myc downstream-regulated gene 2. Surg Res, 2015, 194(1): 273-280.
7.	楊波, 張衛東, 夏彥民, 等. 鹽酸羥考酮注射液對大鼠急性肺損傷的保護作用及機制分析. 中華醫學雜志, 2016, 96(31): 2498-2501.
8.	王牧野, 廖辰, 王麗娟, 等. 內皮祖細胞減少肺移植后的缺血-再灌注損傷. 中國胸心血管外科臨床雜志, 2018, 25(7): 610-615.
9.	Amodio N, Raimondi L, Juli G, et al. MALAT1: druggable long non-coding RNA for targeted anti-cancer approaches. Hematol Oncol, 2018, 11(1): 63.
10.	Sun X, Xu Y, Zhang S, et al. MicroRNA-183 suppresses the vitality, invasion and migration of human osteosarcoma cells by targeting metastasis-associated protein 1. Exp Ther Med, 2018, 15(6): 5058-5064.
11.	Chen JK, Wang WC, Zang L, et al. Repression of chromatin modifier aggravates lipopolysaccharide-induced acute lung injury in mouse. Biochem Biophys Res Commun, 2016, 471(4): 515-521.
12.	Li DQ, Pakala SB, Nair SS, et al. Metastasis-associated protein 1/nucleosome remodeling and histone deacetylase complex in cancer. Cancer Res, 2012, 72(2): 387-394.
13.	周福平, 楊喜晶, 王真, 等. 脾多肽注射液聯合經導管肝動脈化療栓塞治療中晚期原發性肝癌. 第二軍醫大學學報, 2017, 38(3): 379-382.
14.	嚴虹霞, 杜娟, 柴麗敏, 等. 不同免疫增強劑對腫瘤患者免疫功能的影響. 中國醫院藥學雜志, 2017, 37(5): 454-458.
15.	滿明昊, 李立宏, 楊彥龍, 等. 顱腦損傷術后細胞免疫指標變化及脾多肽對其影響. 中國臨床神經外科雜志, 2016, 21(6): 362-365.
16.	楊波, 張衛東, 李小飛, 等. 大劑量鹽酸氨溴索對大鼠肺缺血再灌注損傷后的肺保護機制. 西安交通大學學報 (醫學版), 2016, 37(3): 367-371.
17.	陳家寬, 王榮, 李建忠, 等. N-myc 下游調節基因-2 過表達對大鼠肺缺血再灌注損傷的影響. 現代生物醫學進展, 2016, 16(15): 2816-2819.

1. Yang B, Li XP, Ni YF, et al. Protective effect of isorhamnetin on lipopolysaccharide-induced acute lung injury in mice. Inflammation, 2016, 39(1): 129-137.
2. Zhou J, Niu G, Pei Y, et al. The effect and clinical efficacy of lienal polypeptide injection combined with FOLFOX chemotherapy regimen in colon cancer patients. Oncol Lett, 2016, 12(5): 3191-3194.
3. Huang XE, Wang L, Ji ZQ, et al. Safety of lienal polypeptide injection combined with chemotherapy in treating patients with advanced cancer. Asian Pac Cancer Prev, 2015, 16(17): 7837-7841.
4. 蘇俊孟, 郭暉, 夏飛, 等. 脾多肽對急性肺損傷大鼠 TLR9 信號相關因子的影響. 貴州醫科大學學報, 2017, 42(3): 296-299.
5. Castillo RL, Carrasco Loza R, Romero-Dapueto C. Pathophysiological approaches of acute respiratory distress syndrome: novel bases for study of lung injury. Open Respir Med J, 2015, 9: 83-91.
6. Yang B, Ni YF, Wang WC, et al. Melatonin attenuates intestinal ischemia--reperfusion-induced lung injury in rats by upregulating N-myc downstream-regulated gene 2. Surg Res, 2015, 194(1): 273-280.
7. 楊波, 張衛東, 夏彥民, 等. 鹽酸羥考酮注射液對大鼠急性肺損傷的保護作用及機制分析. 中華醫學雜志, 2016, 96(31): 2498-2501.
8. 王牧野, 廖辰, 王麗娟, 等. 內皮祖細胞減少肺移植后的缺血-再灌注損傷. 中國胸心血管外科臨床雜志, 2018, 25(7): 610-615.
9. Amodio N, Raimondi L, Juli G, et al. MALAT1: druggable long non-coding RNA for targeted anti-cancer approaches. Hematol Oncol, 2018, 11(1): 63.
10. Sun X, Xu Y, Zhang S, et al. MicroRNA-183 suppresses the vitality, invasion and migration of human osteosarcoma cells by targeting metastasis-associated protein 1. Exp Ther Med, 2018, 15(6): 5058-5064.
11. Chen JK, Wang WC, Zang L, et al. Repression of chromatin modifier aggravates lipopolysaccharide-induced acute lung injury in mouse. Biochem Biophys Res Commun, 2016, 471(4): 515-521.
12. Li DQ, Pakala SB, Nair SS, et al. Metastasis-associated protein 1/nucleosome remodeling and histone deacetylase complex in cancer. Cancer Res, 2012, 72(2): 387-394.
13. 周福平, 楊喜晶, 王真, 等. 脾多肽注射液聯合經導管肝動脈化療栓塞治療中晚期原發性肝癌. 第二軍醫大學學報, 2017, 38(3): 379-382.
14. 嚴虹霞, 杜娟, 柴麗敏, 等. 不同免疫增強劑對腫瘤患者免疫功能的影響. 中國醫院藥學雜志, 2017, 37(5): 454-458.
15. 滿明昊, 李立宏, 楊彥龍, 等. 顱腦損傷術后細胞免疫指標變化及脾多肽對其影響. 中國臨床神經外科雜志, 2016, 21(6): 362-365.
16. 楊波, 張衛東, 李小飛, 等. 大劑量鹽酸氨溴索對大鼠肺缺血再灌注損傷后的肺保護機制. 西安交通大學學報 (醫學版), 2016, 37(3): 367-371.
17. 陳家寬, 王榮, 李建忠, 等. N-myc 下游調節基因-2 過表達對大鼠肺缺血再灌注損傷的影響. 現代生物醫學進展, 2016, 16(15): 2816-2819.

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Lienal polypeptide injection attenuates lipopolysaccharide-induced acute lung injury in rats by metastasis-associated protein 1

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