Effect of Early Coronary Artery Bypass Graft Surgery on Expression Level of Ionophorous Protein at Infracted Border Zone in Dog with Acute Myocardial Infarction_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

LIUHong ¹ , KANHong ² ,  ZHANGWen-feng ¹ , LIShi-jie ¹ , TANGHuai-hao ¹ , LIUZhen-bo ¹ , ZHENGZhi-qiang ¹

1. Department of Cardiothoracic Surgery, The Affiliated Haici Hospital of Medical College, Qingdao University, Qingdao 266033 Shandong, P. R. China;
2. Department of Medical Equipment of The Affiliated Haici Hospital of Medical College, Qingdao University, Qingdao 266033 Shandong, P. R. China;

Corresponding?author：

ZHANGWen-feng, Email: zhangwenfeng66@126.com

Keywords：

Myocardial ischemia; Infracted border zone; Sympathetic nervous; Ionophorous protein

DOI：

10.7507/1007-4848.20150195

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Objective To investigate the effect and significance of early coronary artery bypass graft (CABG) on the expression level of ionophorous protein at infracted border zone (IBZ) in dog with acute myocardial infarction. Methods The anterior descending coronary artery of all thirty healthy mongrel dogs were ligated into myocardial infarction model, whose successful criteria was that the regional myocardium supplied by ligated coronary artery became darker. Coronary artery bypass surgery performed at different time points after myocardial infarction (in the 1st week, the 2nd week, the 4th week, the 6th week respectively) was as an experimental group. While myocardial infarction without coronary artery bypass surgery was set up as a control group. Myocardial tissue without ligation of coronary artery was as a normal group. After 8 weeks, myocardial specimens were cut out in the experimental group and the control group. The local expression levels of ionophorous proteins such as Cav1.2, Kv4.3 and KchIP2 mRNA were detected by means of reverse transcription- polymerase chain reaction (RT-PCR) at normal myocardium and IBZ of the experimental group and the control group. Results Four dogs in every experimental group and all dogs in the control group survived to the end of the study. Three myocardial ion channel proteins expression in the control group were lower than those of the normal group or the experimental group significantly (P<0.01). Cav1.2 mRNA expression in the experimental group in the 4th week or the 6th week was lower than that in the normal group significantly (P<0.05). Kv4.3 and KchIP2 mRNA expression in the experimental group in the 4th week or the 6th week were lower than those in the normal group and the experimental group significantly in the 1st week or the 2nd week (P<0.05). Conclusion Early CABG surgery for acute myocardial infarction could lessen the changes of expression level of ionophorous protein at infracted border zone (IBZ) of dog with acute myocardial infarction. Especially, CABG surgery among two weeks could improve expression level of ionophorous protein, and reduce the effect of ischemia for ionophorous protein and myocardial electrophysiology at IBZ.

Citation： LIUHong, KANHong, ZHANGWen-feng, LIShi-jie, TANGHuai-hao, LIUZhen-bo, ZHENGZhi-qiang. Effect of Early Coronary Artery Bypass Graft Surgery on Expression Level of Ionophorous Protein at Infracted Border Zone in Dog with Acute Myocardial Infarction. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2015, 22(8): 778-782. doi: 10.7507/1007-4848.20150195 Copy

1.	Cao JM, Fishbein MC, Han JB, et al. Relationship between regional cardiac hyperinnervation and ventricular arrhythmia. Circulation, 2000, 101(16):1960-1969.
2.	雷遷, 周成斌. 心肌梗死后交感神經重構防治的研究進展. 嶺南心血管病雜志, 2012, 18(6):645-647.
3.	帕力旦·肉孜, 木胡牙提. 心肌梗死后交感神經重構與室性心律失常. 心血管病學進展, 2013, 34(3):371-374.
4.	Tice DA, Zerbino VR, Isom OW, et al. Coronary artery bypass with freeze-preserved saphenous vein allografts. J Thorac Cardiovasc Surg, 1976, 71 (3):378-382.
5.	Chen PS, Chen LS, Cao JM. Sympathetic nerve sprouting, electrical remodeling and the mechanisms of sudden cardiac death. Cardiovasc Res, 2001, 50:409-416.
6.	劉耿星, 方丁芬, 張麗娟. 冠狀動脈心臟病患者的心臟自主神經功能性重構淺析. 國際病理科學與臨床雜志, 2012, 32(6):508-511.
7.	毛俊, 劉興鵬, Sunny PO. 心臟自主神經重構與心肌梗死后室性心律失常. 心血管病學進展, 2011, 32(3):305-307.
8.	Aimond F, Alvarez JL, Rauzier JM, et al. Ionic basis of ventricular arrhythmias in remodeled rat heart during long-term myocardial infarction. Cardiovasc Res, 1999, 42:402-415.
9.	王曉明, 石紅玲, 崔吉君. 心肌梗死后自主神經功能失調與室性心律失常. 中國循證心血管醫學雜志, 2012, 4(4):385-387.
10.	張文峰, 谷天祥, 鄭志強, 等. 犬急性心肌梗死早期冠狀動脈旁路移植術對梗死周邊區心肌神經纖維密度的影響. 中國心血管雜志, 2012, 17(1):38-42.
11.	Nori SL, Gaudino M, Alessandrini F, et al. Immunohistochemical evidence for sympathetic denervation and reinnervation after necrotic injury in rat myocardium. Cell Mol Biol (Noisy-le-grand), 1995, 41:799-807.
12.	Heumann R, Korsching S, Bandtlow C, et al. Changes of nerve growth factor synthesis in nonneuronal cells in response to sciatic nerve transection. J Cell Biol, 1987, 104:1623-1631.
13.	Dae MW, Lee RJ, Ursell PC, et al. Heterogeneous sympathetic innervation in German shepherd dogs with inherited ventricular arrhythmia and sudden cardiac death. Circulation, 1997, 96:1337-1342.
14.	Cao JM, Chen LS, Ken Knight BH. Nerve sprouting and sudden cardiac death. Circ Res, 2000, 86:816-821.
15.	張濤, 商麗華. 心肌梗死后交感神經重構與室性心律失常. 中國心臟起搏與心電生理雜志, 2012, 26(5):383-385.
16.	Pinto JM, Boyden PA. Electrical remodeling in ischemia an infarction. Cardiovasc Res, 1999, 42(2):284.
17.	張文峰, 谷天祥, 呂英義, 等. 冠狀動脈旁路移植術對犬急性心肌梗死心肌神經相關因子表達的影響. 國際外科學雜志, 2011, 38(12):815-819.
18.	張文峰, 谷天祥, 劉泳, 等. 犬急性心肌梗死早期冠狀動脈旁路移植術對左室壁運動的影響. 國際外科學雜志, 2013, 40(3):174-178.
19.	Shen MJ, Shinohara T, Park HW, et al. Continuous low-level vagus nerve stimulation reduces stellate ganglion nerve activity and paroxysmal atrial tachyarrhythmias in ambulatory canines. Circulation, 2011, 123(20):2204-2212.
20.	Yang SS, Han W, Cao Y, et al. Effects of high thoracic epidural anesthesia on atrial electrophysiological characteristics and sympathetic nerve sprouting in a canine model of atrial fibrillation. Basic Res Cardiol, 2011, 106(3):495-506.
21.	Yu T, Zhu W, Gu B, et al. Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process. J Appl Physiol, 2012, 113(12):1937-1944.
22.	李榮, 谷天祥, 李衛國, 等. 早期冠狀動脈搭橋術對犬急性心肌梗死的范圍和腎素系統的影響. 心肺血管病雜志, 2009, 28(1):43-46.
23.	谷天祥, 王春, 張玉海, 等. 非體外循環多支血管病變急性心肌梗死的外科治療. 中華胸心血管外科雜志, 2006, 22(1):15-17.
24.	Rosati B, Pan Z, Lypen S, et al. Regulation of KChIP2 potassium channel subunit gene expression underlies the gradient of transient outward current in canine and human ventricle. J Physiol, 2001, 533(1):119-125.
25.	董炳慶, 閆素華, 王曄. 糖尿病兔心肌梗死后鉀離子通道蛋白基因表達的變化. 中國病理生理雜志, 2011, 27(9):1777-1780.

1. Cao JM, Fishbein MC, Han JB, et al. Relationship between regional cardiac hyperinnervation and ventricular arrhythmia. Circulation, 2000, 101(16):1960-1969.
2. 雷遷, 周成斌. 心肌梗死后交感神經重構防治的研究進展. 嶺南心血管病雜志, 2012, 18(6):645-647.
3. 帕力旦·肉孜, 木胡牙提. 心肌梗死后交感神經重構與室性心律失常. 心血管病學進展, 2013, 34(3):371-374.
4. Tice DA, Zerbino VR, Isom OW, et al. Coronary artery bypass with freeze-preserved saphenous vein allografts. J Thorac Cardiovasc Surg, 1976, 71 (3):378-382.
5. Chen PS, Chen LS, Cao JM. Sympathetic nerve sprouting, electrical remodeling and the mechanisms of sudden cardiac death. Cardiovasc Res, 2001, 50:409-416.
6. 劉耿星, 方丁芬, 張麗娟. 冠狀動脈心臟病患者的心臟自主神經功能性重構淺析. 國際病理科學與臨床雜志, 2012, 32(6):508-511.
7. 毛俊, 劉興鵬, Sunny PO. 心臟自主神經重構與心肌梗死后室性心律失常. 心血管病學進展, 2011, 32(3):305-307.
8. Aimond F, Alvarez JL, Rauzier JM, et al. Ionic basis of ventricular arrhythmias in remodeled rat heart during long-term myocardial infarction. Cardiovasc Res, 1999, 42:402-415.
9. 王曉明, 石紅玲, 崔吉君. 心肌梗死后自主神經功能失調與室性心律失常. 中國循證心血管醫學雜志, 2012, 4(4):385-387.
10. 張文峰, 谷天祥, 鄭志強, 等. 犬急性心肌梗死早期冠狀動脈旁路移植術對梗死周邊區心肌神經纖維密度的影響. 中國心血管雜志, 2012, 17(1):38-42.
11. Nori SL, Gaudino M, Alessandrini F, et al. Immunohistochemical evidence for sympathetic denervation and reinnervation after necrotic injury in rat myocardium. Cell Mol Biol (Noisy-le-grand), 1995, 41:799-807.
12. Heumann R, Korsching S, Bandtlow C, et al. Changes of nerve growth factor synthesis in nonneuronal cells in response to sciatic nerve transection. J Cell Biol, 1987, 104:1623-1631.
13. Dae MW, Lee RJ, Ursell PC, et al. Heterogeneous sympathetic innervation in German shepherd dogs with inherited ventricular arrhythmia and sudden cardiac death. Circulation, 1997, 96:1337-1342.
14. Cao JM, Chen LS, Ken Knight BH. Nerve sprouting and sudden cardiac death. Circ Res, 2000, 86:816-821.
15. 張濤, 商麗華. 心肌梗死后交感神經重構與室性心律失常. 中國心臟起搏與心電生理雜志, 2012, 26(5):383-385.
16. Pinto JM, Boyden PA. Electrical remodeling in ischemia an infarction. Cardiovasc Res, 1999, 42(2):284.
17. 張文峰, 谷天祥, 呂英義, 等. 冠狀動脈旁路移植術對犬急性心肌梗死心肌神經相關因子表達的影響. 國際外科學雜志, 2011, 38(12):815-819.
18. 張文峰, 谷天祥, 劉泳, 等. 犬急性心肌梗死早期冠狀動脈旁路移植術對左室壁運動的影響. 國際外科學雜志, 2013, 40(3):174-178.
19. Shen MJ, Shinohara T, Park HW, et al. Continuous low-level vagus nerve stimulation reduces stellate ganglion nerve activity and paroxysmal atrial tachyarrhythmias in ambulatory canines. Circulation, 2011, 123(20):2204-2212.
20. Yang SS, Han W, Cao Y, et al. Effects of high thoracic epidural anesthesia on atrial electrophysiological characteristics and sympathetic nerve sprouting in a canine model of atrial fibrillation. Basic Res Cardiol, 2011, 106(3):495-506.
21. Yu T, Zhu W, Gu B, et al. Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process. J Appl Physiol, 2012, 113(12):1937-1944.
22. 李榮, 谷天祥, 李衛國, 等. 早期冠狀動脈搭橋術對犬急性心肌梗死的范圍和腎素系統的影響. 心肺血管病雜志, 2009, 28(1):43-46.
23. 谷天祥, 王春, 張玉海, 等. 非體外循環多支血管病變急性心肌梗死的外科治療. 中華胸心血管外科雜志, 2006, 22(1):15-17.
24. Rosati B, Pan Z, Lypen S, et al. Regulation of KChIP2 potassium channel subunit gene expression underlies the gradient of transient outward current in canine and human ventricle. J Physiol, 2001, 533(1):119-125.
25. 董炳慶, 閆素華, 王曄. 糖尿病兔心肌梗死后鉀離子通道蛋白基因表達的變化. 中國病理生理雜志, 2011, 27(9):1777-1780.

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Effect of Early Coronary Artery Bypass Graft Surgery on Expression Level of Ionophorous Protein at Infracted Border Zone in Dog with Acute Myocardial Infarction

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