Experimental study on reconstruction of anterior labrum of shoulder joint by chemical extraction of allogeneic tendon and allogeneic chondrocytes_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

CHEN Guofei ¹ , CUI Lei ² , CHEN Peng ² , LI Wei ² , YOU Tian ² , WANG Chen ² ,  JIANG Changqing ² , LIU Gang ¹

1. Department of Traumatic Osteoarthritis, Shenzhen Hospital, University of Chinese Academy of Sciences, Shenzhen Guangdong, 518000, P.R.China;
2. Department of Sports Medicine, Shenzhen Hospital, Peking University, Shenzhen Guangdong, 518036, P.R.China;

Corresponding?author：

JIANG Changqing, Email: 853655760@qq.com

Keywords：

Chemical extraction; allogeneic tendon; chondrocytes; glenoid labrum reconstruction; rabbittissue engineering; rabbit

DOI：

10.7507/1002-1892.201911156

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Objective To study the effect of chemical extraction of allogeneic tendon and allogeneic chondrocytes for reconstruction of anterior labrum of shoulder joint in rabbits.Methods The body weight of 45 adult New Zealand white rabbits ranged from 2.5 to 3.0 kg. The Achilles tendons of 15 rabbits were taken and the allogeneic tendons were prepared by chemical extraction with antigen inactivation. The extracted tendons were compared with untreated tendons by HE and Masson stainings. Chondrocytes were isolated and cultured by trypsin method and identified by immunohistochemical staining of collagen type Ⅱ. The remaining 30 rabbits were used to prepare the model of anterior labrum defect of shoulder joint. After the allogeneic tendon was transplanted to the damaged labrum, the rabbits was randomly divided into two groups (15 in each group). In group A, the allogeneic chondrocytes were injected into the joint immediately after transplantation, while in group B, no treatment was made. At 4, 6, and 8 weeks after operation, 5 transplanted tendons of each group were taken. After general observation, HE staining was used to observe the number of nuclei, Masson staining was used to observe the expression of collagen fibers in muscle fiber tissues, and AB staining was used to detect the glycosaminoglycan level after transplantation, to evaluate the cell growth in the tissues of the two groups of allogeneic tendon.Results By HE and Masson stainings, the allogeneic tendon antigen prepared by chemical extraction method was inactivated and the fibrous tissue structure was intact; collagen type Ⅱ immunohisto-chemistry staining showed that the cultured cells were chondrocytes. After tendon transplantation, the content of glycosaminoglycan in group A was significantly higher than that in group B (P<0.05). At 6 weeks after operation, HE staining showed that the nuclear in tendon tissue of group A was significantly more than that of group B (t=20.043, P=0.000). Masson staining showed that the number of nuclei in tendon tissue of group A was significantly increased, the muscle fibers and collagen fibers were interlaced, the tissue structure was more compact, and the tendon tissue was mainly blue stained; while the number of nuclei in group B was less, mainly collagen fibers of the original graft.Conclusion The allogeneic tendon inactivated by chemical extraction can be used to reconstruct the defect of anterior labrum of shoulder joint in rabbits, and the combination of allogeneic chondrocytes can promote the healing of tendon transplantation.

Citation： CHEN Guofei, CUI Lei, CHEN Peng, LI Wei, YOU Tian, WANG Chen, JIANG Changqing, LIU Gang. Experimental study on reconstruction of anterior labrum of shoulder joint by chemical extraction of allogeneic tendon and allogeneic chondrocytes. Chinese Journal of Reparative and Reconstructive Surgery, 2020, 34(9): 1184-1189. doi: 10.7507/1002-1892.201911156 Copy

1.	Bottoni CR, Wilckens JH, DeBerardino TM, et al. A prospective, randomized evaluation of arthroscopic stabilization versus nonoperative treatment in patients with acute, traumatic, first-time shoulder dislocations. Am J Sports Med, 2002, 30(4): 576-580.
2.	Trojan JD, Meyer LE, Edgar CM, et al. Epidemiology of shoulder instability injuries in collision collegiate sports from 2009 to 2014. Arthroscopy, 2020, 36(1): 36-43.
3.	Kane P, Bifano SM, Dodson CC, et al. Approach to the treatment of primary anterior shoulder dislocation: A review. Phys Sportsmed, 2015, 43(1): 54-64.
4.	Paulos LE, Evans IK, Pinkowski JL. Anterior and anterior-inferior shoulder instability: Treatment by glenoid labrum reconstruction and a modified capsular shift procedure. J Shoulder Elbow Surg, 1993, 2(6): 305-313.
5.	Bateman E, Donald SM. Reconstruction of massive uncontained glenoid defects using a combined autograft-allograft construct with reverse shoulder arthroplasty: preliminary results. J Shoulder Elbow Surg, 2012, 21(7): 925-934.
6.	肖健, 敖英芳, 王健全, 等. 同種異體半月板移植重建肩關節前盂唇的組織學研究. 中國微創外科雜志, 2013, 13(4): 349-351, 356.
7.	潘丕春, 劉金釗, 孫康. γ 射線照射與非照射人肌腱生物力學的比較研究. 中國矯形外科雜志, 2009, 17(13): 1010-1012.
8.	陳鵬, 江長青, 陳國飛, 等. 不同化學萃取次數對同種異體肌腱生物學性能的影響. 實用手外科雜志, 2017, 31(3): 355-358.
9.	Abe H, Itoi E, Yamamoto N, et al. Healing processes of the glenoid labrallesion in a rabbit model of shoulder dislocation. Tohoku J Exp Med, 2012, 228: 103-108.
10.	Provencher MT, Ghodadra N, Romeo AA. Arthroscopic management of anterior instability: pearls, pitfalls, and lessons learned. Orthop Clin North Am, 2010, 41(3): 325-337.
11.	Qu J, van Alphen NA, Thoreson AR, et al. Effects of trypsinization and mineralization on intrasynovial tendon allograft healing to bone. J Orthop Res, 2015, 33(4): 468-474.
12.	謝磊, 陳百成, 王曉峰, 等. 自體及同種異體肌腱重建前交叉韌帶術后本體感覺恢復的比較研究. 中國修復重建外科雜志, 2011, 25(8): 907-911.
13.	Song YJ, Hua YH. Tendon allograft for treatment of chronic Achilles tendon rupture: A systematic review. Foot Ankle Surg, 2019, 25(3): 252-257.
14.	吳延平, 王英振, 焦兆德, 等. 反復凍融處理同種異體移植兔跟腱組織學及生物力學變化. 中國組織工程研究與臨床康復, 2009, 13(5): 816-820.
15.	姜煒鵬, 左金華, 李繼奎, 等. 以 Triton X-100 和脫氧膽酸鈉為萃取劑制備兔脫細胞神經基質: 有最佳時間嗎? 中國組織工程研究與臨床康復, 2009, 13(47): 9241-9244.
16.	王斌, 席智杰, 梁倩倩, 等. 基質誘導自體軟骨細胞移植治療股骨滑車軟骨損傷. 中國修復重建外科雜志, 2017, 31(1): 98-104.
17.	林祥龍, 高麗蘭, 李瑞欣, 等. 壓縮載荷下軟骨細胞/復合支架的三維培養. 中國組織工程研究, 2019, 23(10): 1483-1488.
18.	林建華, 陳曉東, 鄧凌霄, 等. 大鼠軟骨細胞復制性老化的體外觀察. 中國修復重建外科雜志, 2007, 21(11): 1228-1232.
19.	Lee SU, Lee JY, Joo SY, et al. Transplantation of a scaffold-free cartilage tissue analogue for the treatment of physeal cartilage injury of the proximal tibia in rabbits. Yonsei Med J, 2016, 57(2): 441-448.
20.	林奕鵬, 李韜, 熊燕, 等. 膝關節自體軟骨細胞移植術后康復的研究進展. 中國修復重建外科雜志, 2018, 32(6): 758-763.

1. Bottoni CR, Wilckens JH, DeBerardino TM, et al. A prospective, randomized evaluation of arthroscopic stabilization versus nonoperative treatment in patients with acute, traumatic, first-time shoulder dislocations. Am J Sports Med, 2002, 30(4): 576-580.
2. Trojan JD, Meyer LE, Edgar CM, et al. Epidemiology of shoulder instability injuries in collision collegiate sports from 2009 to 2014. Arthroscopy, 2020, 36(1): 36-43.
3. Kane P, Bifano SM, Dodson CC, et al. Approach to the treatment of primary anterior shoulder dislocation: A review. Phys Sportsmed, 2015, 43(1): 54-64.
4. Paulos LE, Evans IK, Pinkowski JL. Anterior and anterior-inferior shoulder instability: Treatment by glenoid labrum reconstruction and a modified capsular shift procedure. J Shoulder Elbow Surg, 1993, 2(6): 305-313.
5. Bateman E, Donald SM. Reconstruction of massive uncontained glenoid defects using a combined autograft-allograft construct with reverse shoulder arthroplasty: preliminary results. J Shoulder Elbow Surg, 2012, 21(7): 925-934.
6. 肖健, 敖英芳, 王健全, 等. 同種異體半月板移植重建肩關節前盂唇的組織學研究. 中國微創外科雜志, 2013, 13(4): 349-351, 356.
7. 潘丕春, 劉金釗, 孫康. γ 射線照射與非照射人肌腱生物力學的比較研究. 中國矯形外科雜志, 2009, 17(13): 1010-1012.
8. 陳鵬, 江長青, 陳國飛, 等. 不同化學萃取次數對同種異體肌腱生物學性能的影響. 實用手外科雜志, 2017, 31(3): 355-358.
9. Abe H, Itoi E, Yamamoto N, et al. Healing processes of the glenoid labrallesion in a rabbit model of shoulder dislocation. Tohoku J Exp Med, 2012, 228: 103-108.
10. Provencher MT, Ghodadra N, Romeo AA. Arthroscopic management of anterior instability: pearls, pitfalls, and lessons learned. Orthop Clin North Am, 2010, 41(3): 325-337.
11. Qu J, van Alphen NA, Thoreson AR, et al. Effects of trypsinization and mineralization on intrasynovial tendon allograft healing to bone. J Orthop Res, 2015, 33(4): 468-474.
12. 謝磊, 陳百成, 王曉峰, 等. 自體及同種異體肌腱重建前交叉韌帶術后本體感覺恢復的比較研究. 中國修復重建外科雜志, 2011, 25(8): 907-911.
13. Song YJ, Hua YH. Tendon allograft for treatment of chronic Achilles tendon rupture: A systematic review. Foot Ankle Surg, 2019, 25(3): 252-257.
14. 吳延平, 王英振, 焦兆德, 等. 反復凍融處理同種異體移植兔跟腱組織學及生物力學變化. 中國組織工程研究與臨床康復, 2009, 13(5): 816-820.
15. 姜煒鵬, 左金華, 李繼奎, 等. 以 Triton X-100 和脫氧膽酸鈉為萃取劑制備兔脫細胞神經基質: 有最佳時間嗎? 中國組織工程研究與臨床康復, 2009, 13(47): 9241-9244.
16. 王斌, 席智杰, 梁倩倩, 等. 基質誘導自體軟骨細胞移植治療股骨滑車軟骨損傷. 中國修復重建外科雜志, 2017, 31(1): 98-104.
17. 林祥龍, 高麗蘭, 李瑞欣, 等. 壓縮載荷下軟骨細胞/復合支架的三維培養. 中國組織工程研究, 2019, 23(10): 1483-1488.
18. 林建華, 陳曉東, 鄧凌霄, 等. 大鼠軟骨細胞復制性老化的體外觀察. 中國修復重建外科雜志, 2007, 21(11): 1228-1232.
19. Lee SU, Lee JY, Joo SY, et al. Transplantation of a scaffold-free cartilage tissue analogue for the treatment of physeal cartilage injury of the proximal tibia in rabbits. Yonsei Med J, 2016, 57(2): 441-448.
20. 林奕鵬, 李韜, 熊燕, 等. 膝關節自體軟骨細胞移植術后康復的研究進展. 中國修復重建外科雜志, 2018, 32(6): 758-763.

Chinese Journal of Reparative and Reconstructive Surgery

Experimental study on reconstruction of anterior labrum of shoulder joint by chemical extraction of allogeneic tendon and allogeneic chondrocytes

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