Preliminary study on true bone ceramics for alveolar ridge preservation in dogs_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LI Jing , QIAO Wei , REN Xiaoqi , SHI Hao , YANG Ting , MA Shaoying ,  ZHAO Yaping , SU Chengzhong

Shanxi Province Tissue Bank of China Institute for Radiation Protection, Shanxi Osteorad Biomaterial Co.Ltd, Taiyuan Shanxi, 030006, P.R.China;

Corresponding?author：

ZHAO Yaping, Email: zypsir@qq.com

Keywords：

True bone ceramics; canine extraction socket filling; gray scale ratio; alveolar ridge preservation

DOI：

10.7507/1002-1892.201908052

Video：

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Objective To study the preservation effect of true bone ceramics (TBC) prepared by high-temperature calcination of bovine bone on alveolar ridge of canine extraction socket.Methods Six healthy Beagle dogs (aged 1.5-2 years) were selected to extract the second and fourth premolars of both mandibles and the second premolars of the maxilla. The left extraction socket was implanted with TBC as the experimental group, and the right side was implanted with the calcined bovine bone (CBB) as the control group, to observe the alveolar ridge preservation effect. Three dogs were euthanized after general observation at 1 and 6 months after operation respectively. After separating the maxilla and mandible, cone beam CT (CBCT) was performed to measure the average gray value of the graft site and the adjacent reference area (the area between the roots of the adjacent third premolar) and calculate the gray scale ratio between the bone graft site and the reference area. Histological observation was made on the bone graft site to evaluate the new bone formation.Results General observation showed that the wounds of both groups were basically healed at 2 weeks after operation, and the bone graft materials were not exposed. The wounds healed well at 1 and 6 months after operation without swelling. The results of CBCT showed that the residual material was found in both groups at 1 month after operation, and no significant residual material was found in both groups at 6 months after operation, and the alveolar ridge height of the bone graft area was not significantly reduced. There was no significant difference in the bone mineral density between the experimental group and the control group. The gray scale ratios of the experimental group at 1 month and 6 months after operation were 0.97±0.14 and 0.93±0.06, respectively, and were 0.99±0.16 and 0.94±0.05 in control group, showing no significant difference between the two groups (t=?1.030, P=0.333; t=?0.770, P=0.466). HE staining observation showed that a large number of bone graft materials did not degrade and new bone formed around the grafts in both groups at 1 month after operation; the bone graft materials were absorbed and a large number of new bones were formed in both groups at 6 months after operation.Conclusion TBC can maintain bone mineral density and have good osteoconductivity in the alveolar ridge site preservation experiment of dogs, and can be used for alveolar ridge site preservation.

Citation： LI Jing, QIAO Wei, REN Xiaoqi, SHI Hao, YANG Ting, MA Shaoying, ZHAO Yaping, SU Chengzhong. Preliminary study on true bone ceramics for alveolar ridge preservation in dogs. Chinese Journal of Reparative and Reconstructive Surgery, 2019, 33(11): 1452-1456. doi: 10.7507/1002-1892.201908052 Copy

1.	劉偉, 丁宇翔, 秦瑞峰, 等. 骨引導天然煅燒骨與 Bio-Oss 用于牙槽嵴保存效果的對比研究. 實用口腔醫學雜志, 2014, 30(4): 477-481.
2.	Vignoletti F, Matesanz P, Rodrigo D, et al. Surgical protocols for ridge preservation after tooth extraction. A systematic review. Clin Oral Implants Res, 2012, 23 Suppl 5: 22-38.
3.	Zhang W, Li G, Deng R, et al. New bone formation in a true bone ceramic scaffold loaded with desferrioxamine in the treatment of segmental bone defect: a preliminary study. J Orthop Sci, 2012, 17(3): 289-298.
4.	Ooi CY, Hamdi M, Ramesh S. Properties of hydroxyapatite produced by annealing of bovine bone. Ceram Int, 2007, 33(7): 1171-1177.
5.	Joschek S, Nies B, Krotz R, et al. Chemical and physicochemical characterization of porous hydroxyapatite ceramics made of natural bone. Biomaterials, 2000, 21(16): 1645-1658.
6.	高心, 何鐘勤, 王成坤, 等. 骨缺損修復材料煅燒牛骨粉的理化特性. 吉林大學學報(醫學版), 2009, 35(1): 138-141.
7.	喬瑋, 任曉琦, 石浩, 等. 煅燒異種骨的生物相容性研究. 中國修復重建外科雜志, 2017, 31(10): 1250-1255.
8.	Liu Bun-yu, Guo Min-fang, Xing Yan-xia, et al. Safty of true bone ceramics. 中國組織工程研究, 2013, 17(16): 2874-2881.
9.	何越, 田智泉, 王亮, 等. 天然煅燒骨修復材料(骼瑞)修復動物骨缺損的有效性研究. 實用口腔醫學雜志, 2015, 31(2): 167-170.
10.	陳春芳. 陸眠寧聯合戊巴比妥鈉對犬麻醉效果評價. 湘南學院學報(醫學版), 2014, 16(3): 49-50.
11.	Araújo MG, Lindhe J. Dimensional ridge alterations following tooth extraction. An experimental study in the dog. J Clin Periodontol, 2005, 32(2): 212-218.
12.	宋亞平, 徐世同, 劉虎, 等. 4 種骨移植材料修復骨缺損動物模型的建立. 廣東牙病防治, 2014, 22(5): 229-233.
13.	Vignoletti F, Johansson C, Albrekstsson T, et al. Early healing of implants placed into fresh extraction sockets: an experimental study in the beagle dog. De novo bone formation. J Clin Periodontol, 2010, 36(3): 265-277.
14.	Santos FA, Pochapski MT, Martins MC, et al. Comparison of biomaterial implants in the dental socket: histological analysis in dogs. Clin Implant Dent Relat Res, 2010, 12(1): 18-25.
15.	Fickl S, Zuhr O, Wachtel H, et al. Hard tissue alterations after socket preservation: an experimental study in the beagle dog. Clin Oral Implants Res, 2008, 19(11): 1111-1118.
16.	Fickl S, Zuhr O, Wachtel H, et al. Tissue alterations after tooth extraction with and without surgical trauma: a volumetric study in the beagle dog. J Clin Periodontol, 2008, 35(4): 356-363.
17.	連珊, 卿玲, 謝宇, 等. 牙槽嵴位點保存的動物實驗研究. 實用醫學雜志, 2014, 30(12): 1883-1886.
18.	Carter JB, Stone JD, Clark RS, et al. Applications of cone-beam computed tomography in oral and maxillofacial surgery: an overview of published indications and clinical usage in United States Academic Centers and Oral and Maxillofacial Surgery Practices. J Oral Maxillofac Surg, 2016, 74(4): 668-679.
19.	熊艷芳. 錐形束 CT 在慢性牙周炎患者牙周基礎治療后評估牙槽骨變化中的應用價值分析. 當代醫學, 2018, 24(28): 40-42.
20.	Miracle AC, Mukherji SK. Conebeam CT of the head and neck, part 1: physical principles. AJNR Am J Neuroradiol, 2009, 30(6): 1088-1095.

1. 劉偉, 丁宇翔, 秦瑞峰, 等. 骨引導天然煅燒骨與 Bio-Oss 用于牙槽嵴保存效果的對比研究. 實用口腔醫學雜志, 2014, 30(4): 477-481.
2. Vignoletti F, Matesanz P, Rodrigo D, et al. Surgical protocols for ridge preservation after tooth extraction. A systematic review. Clin Oral Implants Res, 2012, 23 Suppl 5: 22-38.
3. Zhang W, Li G, Deng R, et al. New bone formation in a true bone ceramic scaffold loaded with desferrioxamine in the treatment of segmental bone defect: a preliminary study. J Orthop Sci, 2012, 17(3): 289-298.
4. Ooi CY, Hamdi M, Ramesh S. Properties of hydroxyapatite produced by annealing of bovine bone. Ceram Int, 2007, 33(7): 1171-1177.
5. Joschek S, Nies B, Krotz R, et al. Chemical and physicochemical characterization of porous hydroxyapatite ceramics made of natural bone. Biomaterials, 2000, 21(16): 1645-1658.
6. 高心, 何鐘勤, 王成坤, 等. 骨缺損修復材料煅燒牛骨粉的理化特性. 吉林大學學報(醫學版), 2009, 35(1): 138-141.
7. 喬瑋, 任曉琦, 石浩, 等. 煅燒異種骨的生物相容性研究. 中國修復重建外科雜志, 2017, 31(10): 1250-1255.
8. Liu Bun-yu, Guo Min-fang, Xing Yan-xia, et al. Safty of true bone ceramics. 中國組織工程研究, 2013, 17(16): 2874-2881.
9. 何越, 田智泉, 王亮, 等. 天然煅燒骨修復材料(骼瑞)修復動物骨缺損的有效性研究. 實用口腔醫學雜志, 2015, 31(2): 167-170.
10. 陳春芳. 陸眠寧聯合戊巴比妥鈉對犬麻醉效果評價. 湘南學院學報(醫學版), 2014, 16(3): 49-50.
11. Araújo MG, Lindhe J. Dimensional ridge alterations following tooth extraction. An experimental study in the dog. J Clin Periodontol, 2005, 32(2): 212-218.
12. 宋亞平, 徐世同, 劉虎, 等. 4 種骨移植材料修復骨缺損動物模型的建立. 廣東牙病防治, 2014, 22(5): 229-233.
13. Vignoletti F, Johansson C, Albrekstsson T, et al. Early healing of implants placed into fresh extraction sockets: an experimental study in the beagle dog. De novo bone formation. J Clin Periodontol, 2010, 36(3): 265-277.
14. Santos FA, Pochapski MT, Martins MC, et al. Comparison of biomaterial implants in the dental socket: histological analysis in dogs. Clin Implant Dent Relat Res, 2010, 12(1): 18-25.
15. Fickl S, Zuhr O, Wachtel H, et al. Hard tissue alterations after socket preservation: an experimental study in the beagle dog. Clin Oral Implants Res, 2008, 19(11): 1111-1118.
16. Fickl S, Zuhr O, Wachtel H, et al. Tissue alterations after tooth extraction with and without surgical trauma: a volumetric study in the beagle dog. J Clin Periodontol, 2008, 35(4): 356-363.
17. 連珊, 卿玲, 謝宇, 等. 牙槽嵴位點保存的動物實驗研究. 實用醫學雜志, 2014, 30(12): 1883-1886.
18. Carter JB, Stone JD, Clark RS, et al. Applications of cone-beam computed tomography in oral and maxillofacial surgery: an overview of published indications and clinical usage in United States Academic Centers and Oral and Maxillofacial Surgery Practices. J Oral Maxillofac Surg, 2016, 74(4): 668-679.
19. 熊艷芳. 錐形束 CT 在慢性牙周炎患者牙周基礎治療后評估牙槽骨變化中的應用價值分析. 當代醫學, 2018, 24(28): 40-42.
20. Miracle AC, Mukherji SK. Conebeam CT of the head and neck, part 1: physical principles. AJNR Am J Neuroradiol, 2009, 30(6): 1088-1095.

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