Effect of collagen peptides from walleye pollock skin on bone microstructure of ovariectomized rats_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LIU Lu ¹ , JIN Lixin ² , YANG Jieru ³ , WU Binbin ⁴ , ZHANG Yimeng ³ , HAN Yantao ⁵ , WANG Chunbo ⁵ ,  XING Lifeng ⁴

1. Institute of Integrative Medicine, Qingdao University Medical College, Qingdao Shandong, 266021, P.R.China;
2. Department of Anatomy, Qingdao University Medical College, Qingdao Shandong, 266071, P.R.China;
3. School of Clinical Medicine, Qingdao University Medical College, Qingdao Shandong, 266071, P.R.China;
4. Department of Joint Surgery, Qingdao Haici Medical Group, Qingdao Shandong, 266033, P.R.China;
5. School of Pharmacy, Qingdao University, Qingdao Shandong, 266071, P.R.China;

Corresponding?author：

XING Lifeng, Email: xlf329@126.com

Keywords：

Collagen peptides from walleye pollock skin; osteoporosis; calcitonin receptor; interleukin 1; bone microstructure; rat

DOI：

10.7507/1002-1892.201704130

Video：

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Objective To investigate the effect of collagen peptides from walleye pollock skin on the microstructure of osteoporosis model in ovariectomized rats, and to explore the feasibility of preventing and treating oste- oporosis. Methods Sixty adult Wistar female rats, weighing (250±10) g, were randomly divided into 5 groups (12 rats each group): normal group (group A), osteoporosis model group (group B), osteoporosis model+collagen peptides from walleye pollock skin prevention group (group C), osteoporosis model+low concentration of collagen peptides from walleye pollock skin treatment group (group D), and osteoporosis model+high concentration of collagen peptides from walleye pollock skin treatment group (group E). The rats in groups B, C, D, and E were removed bilateral ovarian to establish osteoporosis model. The rats in group C were treated with stomach perfusion of the collagen peptides from walleye pollock skin (1.0 g/kg) from 4 weeks after operation for 6 weeks; and the rats in groups D and E were treated with stomach perfusion of the collagen peptides from walleye pollock skin (0.5, 1.0 g/kg respectively) at 6 weeks after operation for 6 weeks. The rats in groups A and B were given equal volume of normal saline at the same time after operation. At 24 hours after the last administration, the femoral gray value of rats in groups A and B were measured by X-ray film; HE staining was performed on the proximal tibial bone of the left side in 4 groups; the histopathological changes of the bone were observed and the trabecular number (TN), mean trabecular plate thickness (MTPT), mean trabecular plate spacing (MTPS), trabecular bone volume (TBV), mean bone cortical thickness (MBCT) were measured; immunohistochemical staining was performed to observe the expression levels of caltitonin receptor (CTR) and interleukin 1 (IL-1). Results The femoral gray value of group B was significantly lower than that of group A (t=45.130, P=0.000), which indicated that the ovariectomized rat model was successfully prepared. Histological observation showed that TN, MTPS, TBV, and MBCT in groups A, C, and E were significantly different from those in group B (P<0.05). The histological parameters of bone tissue in group C were significantly different from those in groups D and E (P<0.05). TN, MTPS, TBV, and MBCT in group D were significantly different from those in group A (P<0.05); only MTPS in group E was significantly different from that in group A (P<0.05). MTPS, TBV, and MBCT in group E were significantly different from those in group D (P<0.05). The immunohistochemical staining showed that the levels of CTR and IL-1 in groups A, C, D, and E were lower than those in group B, in groups C and E were lower than in group D, showing significant differences (P<0.05). Conclusion Collagen peptides from walleye pollock skin can improve the bone microstructure of osteoporotic rats, and its mechanism may be related to the inhibition of CTR and IL-1 expression in bone tissue, but it has not been found to prevent osteoporosis.

Citation： LIU Lu, JIN Lixin, YANG Jieru, WU Binbin, ZHANG Yimeng, HAN Yantao, WANG Chunbo, XING Lifeng. Effect of collagen peptides from walleye pollock skin on bone microstructure of ovariectomized rats. Chinese Journal of Reparative and Reconstructive Surgery, 2017, 31(10): 1214-1219. doi: 10.7507/1002-1892.201704130 Copy

1.	Pallagatti S, Parnami P, Sheikh S, et al. Efficacy of Panoramic Radiography in the Detection of Osteoporosis in Post-Menopausal Women When Compared to Dual Energy X-Ray Absorptiometry. Open Dent J, 2017, 11: 350-359.
2.	Kavitha MS, An SY, An CH, et al. Texture analysis of mandibular cortical bone on digital dental panoramic radiographs for the diagnosis of osteoporosis in Korean women. Oral Surg Oral Med Oral Pathol Oral Radiol, 2015, 119(3): 346-356.
3.	Shang DP, Lian HY, Fu DP, et al. Relationship between estrogen receptor 1 gene polymorphisms and postmenopausal osteoporosis of the spine in Chinese women. Genet Mol Res, 2016, 15(2): 1-6.
4.	吳彬, 張勇, 陳明亮, 等. 雌馬酚對去卵巢后大鼠骨質疏松癥的影響. 第三軍醫大學學報, 2015, 37(3): 256-260.0.
5.	周建烈, 陳悅. 水解膠原蛋白的骨骼和關節效應研究進展. 中國骨質疏松雜志, 2010, 16(10): 798-801.0.
6.	Li X, Shi F, Gong L, et al. Species-specific identification of collagen components in Colla corii asini using a nano-liquid chromatography tandem mass spectrometry proteomics approach. Int J Nanomedicine, 2017, 12: 4443-4454.
7.	Wu H, Ren C, Yang F, et al. Extraction and identification of collagen-derived peptides with hematopoietic activity from Colla Corii Asini. J Ethnopharmacol, 2016, 182: 129-136.
8.	Wang D, Ru W, Xu Y, et al. Chemical constituents and bioactivities of Colla corii asini. Drug Discov Ther, 2014, 8(5): 201-207.
9.	張鶴, 趙雨, 徐云鳳, 等. 鹿骨膠原蛋白對去卵巢所致骨質疏松大鼠的治療作用. 中藥藥理與臨床, 2011, 27(5): 76-79.0.
10.	樊潔, 張德巖, 苗德深, 等. 狹鱈魚皮膠原多肽理化性質研究. 青島大學醫學院學報, 2013, 49(6): 479-481.0.
11.	薛玲芳, 陳雪紅, 徐宏偉, 等. 狹鱈魚皮膠原肽對過氧化氫誘導的人成骨細胞氧化損傷作用. 安徽農業科學, 2016, 44(25): 142-145.0.
12.	盧玉坤. 鱈魚皮膠原蛋白肽的促鈣吸收作用研究. 青島: 中國海洋大學, 2013.
13.	Guo L, Harnedy PA, Zhang L, et al. In vitro assessment of the multifunctional bioactive potential of Alaska pollock skin collagen following simulated gastrointestinal digestion. J Sci Food Agric, 2015, 95(7): 1514-1520.
14.	Beaudreuil J, Taboulet J, Orcel P, et al. Calcitonin receptor mRNA in mononuclear leucocytes from postmenopausal women: decrease during osteoporosis and link to bone markers with specific isoform involvement. Bone, 2000, 27(1): 161-168.
15.	Lin CC, Li TC, Liu CS, et al. Associations of TNF-α and IL-6 polymorphisms with osteoporosis through joint effects and interactions with LEPR gene in Taiwan: Taichung Community Health Study for Elders (TCHS-E). Mol Biol Rep, 2016, 43(10): 1179-1191.
16.	Xu J, Gao Y, Yin J, et al. Calcitonin receptor gene polymorphism in cCinese Xinjiang Han and Uygur women with primary osteoporosis. J Nutr Health Aging, 2014, 18(2): 204-208.
17.	Ito A, Yoshimura M. Mechanisms of the analgesic effect of calcitonin on chronic pain by alteration of receptor or channel expression. Mol Pain, 2017, 13: 1744806917720316.
18.	柴建勝, 鄧高榮, 黃巍. 降鈣素受體在去勢大鼠骨質疏松模型組織中的分布. 醫學信息, 2013, 26(10 上): 141.0.
19.	Gardner CR. Morphological analysis of osteoclastogenesis induced by RANKL in mouse bone marrow cell cultures. Cell Biol Int, 2007, 31(7): 672-682.
20.	Mancini L, Paul-Clark MJ, Rosignoli G, et al. Calcitonin and prednisolone display antagonistic actions on bone and have synergistic effects in experimental arthritis. Am J Pathol, 2007, 170(3): 1018-1027.
21.	Brandstr?m H, Jonsson KB, Ohlsson C, et al. Regulation of osteoprotegerin mRNA levels by prostaglandin E2 in human bone marrow stroma cells. Biochem Biophys Res Commun, 1998, 247(2): 338-341.
22.	Li H, Cuartas E, Cui W, et al. IL-1 receptor-associated kinase M is a central regulator of osteoclast differentiation and activation. J Exp Med, 2005, 201(7): 1169-1177.
23.	李曉紅, 司紅羚, 劉嘯晨. 雌激素對骨質疏松大鼠牙槽骨改建過程中 IL-1 表達的影響. 實用口腔醫學雜志, 2006, 22(5): 684-687.0.
24.	Manolagas SC, Jilka RL. Bone marrow, cytokines, and bone remodeling. Emerging insights into the pathophysiology of osteoporosis. N Engl J Med, 1995, 332(5): 305-311.

1. Pallagatti S, Parnami P, Sheikh S, et al. Efficacy of Panoramic Radiography in the Detection of Osteoporosis in Post-Menopausal Women When Compared to Dual Energy X-Ray Absorptiometry. Open Dent J, 2017, 11: 350-359.
2. Kavitha MS, An SY, An CH, et al. Texture analysis of mandibular cortical bone on digital dental panoramic radiographs for the diagnosis of osteoporosis in Korean women. Oral Surg Oral Med Oral Pathol Oral Radiol, 2015, 119(3): 346-356.
3. Shang DP, Lian HY, Fu DP, et al. Relationship between estrogen receptor 1 gene polymorphisms and postmenopausal osteoporosis of the spine in Chinese women. Genet Mol Res, 2016, 15(2): 1-6.
4. 吳彬, 張勇, 陳明亮, 等. 雌馬酚對去卵巢后大鼠骨質疏松癥的影響. 第三軍醫大學學報, 2015, 37(3): 256-260.0.
5. 周建烈, 陳悅. 水解膠原蛋白的骨骼和關節效應研究進展. 中國骨質疏松雜志, 2010, 16(10): 798-801.0.
6. Li X, Shi F, Gong L, et al. Species-specific identification of collagen components in Colla corii asini using a nano-liquid chromatography tandem mass spectrometry proteomics approach. Int J Nanomedicine, 2017, 12: 4443-4454.
7. Wu H, Ren C, Yang F, et al. Extraction and identification of collagen-derived peptides with hematopoietic activity from Colla Corii Asini. J Ethnopharmacol, 2016, 182: 129-136.
8. Wang D, Ru W, Xu Y, et al. Chemical constituents and bioactivities of Colla corii asini. Drug Discov Ther, 2014, 8(5): 201-207.
9. 張鶴, 趙雨, 徐云鳳, 等. 鹿骨膠原蛋白對去卵巢所致骨質疏松大鼠的治療作用. 中藥藥理與臨床, 2011, 27(5): 76-79.0.
10. 樊潔, 張德巖, 苗德深, 等. 狹鱈魚皮膠原多肽理化性質研究. 青島大學醫學院學報, 2013, 49(6): 479-481.0.
11. 薛玲芳, 陳雪紅, 徐宏偉, 等. 狹鱈魚皮膠原肽對過氧化氫誘導的人成骨細胞氧化損傷作用. 安徽農業科學, 2016, 44(25): 142-145.0.
12. 盧玉坤. 鱈魚皮膠原蛋白肽的促鈣吸收作用研究. 青島: 中國海洋大學, 2013.
13. Guo L, Harnedy PA, Zhang L, et al. In vitro assessment of the multifunctional bioactive potential of Alaska pollock skin collagen following simulated gastrointestinal digestion. J Sci Food Agric, 2015, 95(7): 1514-1520.
14. Beaudreuil J, Taboulet J, Orcel P, et al. Calcitonin receptor mRNA in mononuclear leucocytes from postmenopausal women: decrease during osteoporosis and link to bone markers with specific isoform involvement. Bone, 2000, 27(1): 161-168.
15. Lin CC, Li TC, Liu CS, et al. Associations of TNF-α and IL-6 polymorphisms with osteoporosis through joint effects and interactions with LEPR gene in Taiwan: Taichung Community Health Study for Elders (TCHS-E). Mol Biol Rep, 2016, 43(10): 1179-1191.
16. Xu J, Gao Y, Yin J, et al. Calcitonin receptor gene polymorphism in cCinese Xinjiang Han and Uygur women with primary osteoporosis. J Nutr Health Aging, 2014, 18(2): 204-208.
17. Ito A, Yoshimura M. Mechanisms of the analgesic effect of calcitonin on chronic pain by alteration of receptor or channel expression. Mol Pain, 2017, 13: 1744806917720316.
18. 柴建勝, 鄧高榮, 黃巍. 降鈣素受體在去勢大鼠骨質疏松模型組織中的分布. 醫學信息, 2013, 26(10 上): 141.0.
19. Gardner CR. Morphological analysis of osteoclastogenesis induced by RANKL in mouse bone marrow cell cultures. Cell Biol Int, 2007, 31(7): 672-682.
20. Mancini L, Paul-Clark MJ, Rosignoli G, et al. Calcitonin and prednisolone display antagonistic actions on bone and have synergistic effects in experimental arthritis. Am J Pathol, 2007, 170(3): 1018-1027.
21. Brandstr?m H, Jonsson KB, Ohlsson C, et al. Regulation of osteoprotegerin mRNA levels by prostaglandin E2 in human bone marrow stroma cells. Biochem Biophys Res Commun, 1998, 247(2): 338-341.
22. Li H, Cuartas E, Cui W, et al. IL-1 receptor-associated kinase M is a central regulator of osteoclast differentiation and activation. J Exp Med, 2005, 201(7): 1169-1177.
23. 李曉紅, 司紅羚, 劉嘯晨. 雌激素對骨質疏松大鼠牙槽骨改建過程中 IL-1 表達的影響. 實用口腔醫學雜志, 2006, 22(5): 684-687.0.
24. Manolagas SC, Jilka RL. Bone marrow, cytokines, and bone remodeling. Emerging insights into the pathophysiology of osteoporosis. N Engl J Med, 1995, 332(5): 305-311.

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Effect of collagen peptides from walleye pollock skin on bone microstructure of ovariectomized rats

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