EXPRESSION AND SIGNIFICANCE OF PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR γ IN KELOID_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LEILei ¹ , DENGYiling ² ,  CHENJunjie ¹ ,  CENYing ¹

1. Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, 610041, P. R. China;
2. School of Basic Medical Sciences, Sichuan University;

Corresponding?author：

CHENJunjie, Email: cjjemail@163.com; CENYing, Email: cenying0141@163.com

Keywords：

Keloid; Peroxisome proliferator activated receptor γ; Immunohistochemistry

DOI：

10.7507/1002-1892.20160233

Video：

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Objective To investigate the expression and significance of peroxisome proliferator activated receptor γ(PPAR-γ) in human keloid. Methods Twenty-three keloid samples were harvested from the patients undergoing keloid and auto-skin grafting operation as the experimental group (keloid group), and the residual normal skin after auto-skin grafting operation was collected as the control group. The expression of PPAR-γ protein was examined by immunohistochemistry staining in both keloid and normal skin. Referring to Shimizu immunohistochemical standard, the result was graded; the positive rate of samples and the rate of positive cells were calculated. Results Immunohistochemistry staining showed that PPAR-γ protein was expressed in both keloid and normal skin. In keloid, it located in the pricle cell layer, and granular layer of epidermis, and the dermal vessel; the degree of dyeing was very light. However, in normal skin, it located in the base layer of epidermis, dermal vessel walls, sweat glands and sebaceous glands; the dyeing degree was deeper. Immunohistochemical staining score in the keloid group (2.65±0.78) was significantly lower than that in the control group (3.65±1.19) (t=5.030, P=0.000). The positive rate of samples in the keloid group (52.17%, 12/23) was significantly lower than that in the control group (82.61%, 19/23) (χ²=4.847, P=0.028). The rate of positive cells was 46.04%±8.61% in the keloid group, which was significantly lower than that in the control group (59.39%±11.26%) (t=5.974, P=0.000). Conclusion Compared with normal skin, the expression of PPAR-γ protein in keloid is down-regulated in in human keloid, indicating that PPAR-γ may be related to the formation of keloid.

Citation： LEILei, DENGYiling, CHENJunjie, CENYing. EXPRESSION AND SIGNIFICANCE OF PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR γ IN KELOID. Chinese Journal of Reparative and Reconstructive Surgery, 2016, 30(9): 1143-1145. doi: 10.7507/1002-1892.20160233 Copy

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2.	Reddy RC. Immunomodulatory role of PPAR-gama in alveolarm acrophages. J Investig Med, 2008, 56(2):522-527.
3.	Shimizu M, Saitoh Y, Itoh H. Immunohistochemical staining of Ha-ras oncogene product in normal, benign, and malignant human pancreatic tissues. Hum Pathol, 1990, 21(6):607-612.
4.	Zhang GY, Cheng T, Zheng MH, et al. Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist inhibits transforming growth factor-betal and matrix production in human dermal fibroblasts. J Plast Reconstr Aesthet Surg, 2010, 63(7):1209-1216.
5.	Wu M, Melichian DS, Chang E, et al. Rosiglitazong abrogates bleomycin-induced scleroderma and blocks profibrotic responses through PPAR-γ. Am J Pathol, 2009, 174(2):519-533.
6.	Pascul G, Sullivan AL, Ogawa S, et al. Anti-inflammatory and antidiabetic roles of PPAR-gamma. Novartis Found Symp, 2007, 286:183-203.
7.	潘曉娟, 張光峰, 董光富, 等.過氧化物酶體增殖物激活受體γ在結締組織病相關的肺間質病變中抗纖維化作用機制研究.中華風濕病學雜志, 2013, 17(4):231-235.
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10.	康誼, 曾艷麗, 魏君鋒, 等. PPARγ在大鼠肝纖維化形成過程中的動態變化.基礎醫學與臨床, 2013, 33(10):1324-1325.
11.	黃津, 張一. LX-2細胞中PPARγ對iNOS的影響及其抗肝纖維化作用.中國肝臟病雜志(電子版), 2015, 7(2):59-64.
12.	Miyahara T, Schrum L, Rippe R, et al. Peroxisome proliferator-activated receptors and hepatic stellate cell activation. J Biol Chem, 2000, 275(46):35715-35722.
13.	Guo YT, Leng XS, Li T, et al. Effect of ligand of peroxisome proliferator-activated receptor gamma on biological characters of hepatic stellate cells. World J Gastroenterol, 2005, 11(30):4735-4739.
14.	金國萍, 應利君, 童海明, 等.核受體PPARγ在醛固酮誘導大鼠急性腎損傷中的作用研究.浙江醫學, 2015, 37(14):1196-1198, 1208.
15.	李相友, 丁國華, 夏瑗瑜, 等.血管緊張素1-7對糖尿病大鼠腎小管間質纖維化的影響.中華腎臟病雜志, 2012, 28(10):798-803.
16.	孫鵬.不同劑量PPARγ激動劑對非糖尿病肥胖小鼠血清脂聯素、尿蛋白排泄及腎臟病理改變的相關性研究.天津:天津醫科大學, 2014.
17.	侯定善, 張曉梅.過氧化物酶體增生物激活受體γ及其激動劑在眼科疾病中的作用.中華實驗眼科雜志, 2014, 32(10):954-960.
18.	趙樹梅, 李虹偉, 沈潞華.過氧化物酶體增殖物激活受體γ通路激活對糖尿病大鼠心肌纖維化的影響.中華老年多器官疾病雜志, 2010, 9(6):525-528.
19.	聶樹濤. PPARγ激動劑對增生性瘢痕成纖維細胞TGF-β₁和Smad信號途徑的作用研究.中國美容醫學, 2011, 20(7):1098-1099.
20.	張義鵬, 解學關, 高偉陽, 等. PPAR-γ在增生性瘢痕中的表達及意義.醫學研究雜志, 2012, 41(4):145-148.

1. ?Kersten S, Desvergne B, Wahli W. Roles of PPARs in health and disease. Nature, 2000, 405(6785):421-424.
2. Reddy RC. Immunomodulatory role of PPAR-gama in alveolarm acrophages. J Investig Med, 2008, 56(2):522-527.
3. Shimizu M, Saitoh Y, Itoh H. Immunohistochemical staining of Ha-ras oncogene product in normal, benign, and malignant human pancreatic tissues. Hum Pathol, 1990, 21(6):607-612.
4. Zhang GY, Cheng T, Zheng MH, et al. Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist inhibits transforming growth factor-betal and matrix production in human dermal fibroblasts. J Plast Reconstr Aesthet Surg, 2010, 63(7):1209-1216.
5. Wu M, Melichian DS, Chang E, et al. Rosiglitazong abrogates bleomycin-induced scleroderma and blocks profibrotic responses through PPAR-γ. Am J Pathol, 2009, 174(2):519-533.
6. Pascul G, Sullivan AL, Ogawa S, et al. Anti-inflammatory and antidiabetic roles of PPAR-gamma. Novartis Found Symp, 2007, 286:183-203.
7. 潘曉娟, 張光峰, 董光富, 等.過氧化物酶體增殖物激活受體γ在結締組織病相關的肺間質病變中抗纖維化作用機制研究.中華風濕病學雜志, 2013, 17(4):231-235.
8. 劉強, 王超云, 馬小松, 等.過氧化物酶體增殖物激活受體γ(PPAR-γ)與肝纖維化的研究進展.濱州醫學院學報, 2013, 36(1):49-51.
9. Wang Z, Xu JP, Zheng YC, et al. Peroxisome proliferator-activated receptor gamma inhibits hepatic fibrosis in rats. Hepatob Pancreat Dis Int, 2011, 10(1):64-71.
10. 康誼, 曾艷麗, 魏君鋒, 等. PPARγ在大鼠肝纖維化形成過程中的動態變化.基礎醫學與臨床, 2013, 33(10):1324-1325.
11. 黃津, 張一. LX-2細胞中PPARγ對iNOS的影響及其抗肝纖維化作用.中國肝臟病雜志(電子版), 2015, 7(2):59-64.
12. Miyahara T, Schrum L, Rippe R, et al. Peroxisome proliferator-activated receptors and hepatic stellate cell activation. J Biol Chem, 2000, 275(46):35715-35722.
13. Guo YT, Leng XS, Li T, et al. Effect of ligand of peroxisome proliferator-activated receptor gamma on biological characters of hepatic stellate cells. World J Gastroenterol, 2005, 11(30):4735-4739.
14. 金國萍, 應利君, 童海明, 等.核受體PPARγ在醛固酮誘導大鼠急性腎損傷中的作用研究.浙江醫學, 2015, 37(14):1196-1198, 1208.
15. 李相友, 丁國華, 夏瑗瑜, 等.血管緊張素1-7對糖尿病大鼠腎小管間質纖維化的影響.中華腎臟病雜志, 2012, 28(10):798-803.
16. 孫鵬.不同劑量PPARγ激動劑對非糖尿病肥胖小鼠血清脂聯素、尿蛋白排泄及腎臟病理改變的相關性研究.天津:天津醫科大學, 2014.
17. 侯定善, 張曉梅.過氧化物酶體增生物激活受體γ及其激動劑在眼科疾病中的作用.中華實驗眼科雜志, 2014, 32(10):954-960.
18. 趙樹梅, 李虹偉, 沈潞華.過氧化物酶體增殖物激活受體γ通路激活對糖尿病大鼠心肌纖維化的影響.中華老年多器官疾病雜志, 2010, 9(6):525-528.
19. 聶樹濤. PPARγ激動劑對增生性瘢痕成纖維細胞TGF-β₁和Smad信號途徑的作用研究.中國美容醫學, 2011, 20(7):1098-1099.
20. 張義鵬, 解學關, 高偉陽, 等. PPAR-γ在增生性瘢痕中的表達及意義.醫學研究雜志, 2012, 41(4):145-148.

Chinese Journal of Reparative and Reconstructive Surgery

EXPRESSION AND SIGNIFICANCE OF PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR γ IN KELOID

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