MODEL ESTABLISHMENT, MRI AND PATHOLOGICAL FEATURES OF EARLY STEROID-INDUCED AVASCULAR NECROSIS OF FEMORAL HEAD IN RABBIT_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

ZHANGLiyan ¹ , SUNXin ² , TIANDan ² , XURui ¹ , LEIHao ¹ , AIJinhui ¹ , ZHAOBo ¹ ,  CHENJiying ³ ,  CHAIWei ³ , MAShoucheng ⁴ , LIUWeijia ⁴ , SHENSiyuan ⁴

1. First Department of Orthopaedics, Affiliated Hospital of Beihua University, Jilin Jilin, 132001, P. R. China;
2. Life Science Research Center of Beihua University;
3. Department of Orthopedics, General Hospital of Chinese PLA;
3. Department of MRI, Jilin Hospital of Traditional Chinese and Western Medicine;

Corresponding?author：

CHENJiying, Email: chenjiying_301@163.com; CHAIWei, Email: 5170765@qq.com

Keywords：

Steroid-induced avascular necrosis of femoral head; MRI; Pathological examination; Animal model; Rabbit

DOI：

10.7507/1002-1892.20150269

Video：

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Objective To establish an rabbit model of early steroid-induced avascular necrosis of the femoral head (SANFH) and evaluate its validity with MRI and pathological examination. Methods Twenty 6-month-old rabbits (weighing, 2-3 kg) were randomly divided into 2 groups (control group and model group), 10 rabbits in each group. Dexamethasone sodium phosphate solution (10 mg/kg) was injected into bilateral gluteus in model group, and the same amount of saline was injected in control group, every 3 days for 14 times. General observation was done after modelling. Osteonecrosis was verified by pathological observation and MRI findings at 6 weeks. Results After 6 weeks, rabbits did not show obvious changes in control group; increased hair removal, decreased food intake, and slight limp were observed in model group. The MRI results showed normal shape of the bilateral femoral head and no abnormal signals in control group; irregular shape of the bilateral femoral head and a slice of irregular abnormal signals were observed, and necrosis and cystolization of the subchondral bone and sparse changes of trabecular bone were shown in model group. General observation from coronal section of femoral head showed smooth red cartilage surface in control group; on the contrary, the cartilage surface of the femoral head became dull, thin even visible hemorrhage under articular cartilage and necrosis of the femoral head were observed. The histopathological examination indicated that trabecular bone of the femoral head in control group was massive, thick, and close, and osteocytes in the bone lacunae had normal shapes. The osseous trabecular became thinner and broken; karyopyknosis of osteocytes and bone empty lacunae could be obviously seen in model group. The rates of empty lacunae were 8.0%±0.5% in control group and 49.0%±0.3% in model group, showing significant difference (t=21.940, P=0.000). Conclusion Establishing a model of early SANFH through injecting shortterm, shock, and high dose of dexamethasone, and it can been evaluated effectively with MRI and pathological examination.

Citation： ZHANGLiyan, SUNXin, TIANDan, XURui, LEIHao, AIJinhui, ZHAOBo, CHENJiying, CHAIWei, MAShoucheng, LIUWeijia, SHENSiyuan. MODEL ESTABLISHMENT, MRI AND PATHOLOGICAL FEATURES OF EARLY STEROID-INDUCED AVASCULAR NECROSIS OF FEMORAL HEAD IN RABBIT. Chinese Journal of Reparative and Reconstructive Surgery, 2015, 29(10): 1240-1243. doi: 10.7507/1002-1892.20150269 Copy

1.	Wang XS, Zhuang QY, Weng XS, et al. Etiological and clinical analysis of osteonecrosis of the femoral head in Chinese patients. Chin Med J (Engl), 2013, 126(2):290-295.
2.	周磊, 翁習生. 激素性股骨頭缺血性壞死動物模型的選擇與應用. 中國矯形外科雜志, 2013, 21(21):2155-2158.
3.	Guan XY, Han D. Role of hypercoagulability in steroid-induced femoral head necrosis in rabbits. J Orthop Sci, 2010, 15(3):365-370.
4.	董玉雷, 周磊, 李玉龍, 等. 大鼠激素性股骨頭缺血性壞死模型的建立和評價. 中國醫學科學院學報, 2015, 37(2):152-156.
5.	張國橋, 易誠青, 滕松松, 等. 早期激素性股骨頭缺血性壞死模型的建立. 現代生物醫學進展, 2012, 12(7):1223-1224.
6.	李超, 尚希福, 李旭, 等. 提高激素性股骨頭缺血性壞死兔模型動物存活率的實驗研究. 安徽醫科大學學報, 2014, 49(12):1726-1729.
7.	楊帆, 朱振中, 李廣翼, 等. 股骨頭缺血性壞死病理形態學研究進展. 國際骨科學雜志, 2014, 35(5):313-315.
8.	Wen Q, Ma L, Chen YP, et al. A rabbit model of hormone-induced early avascular necrosis of the femoral head. Biomed Environ Sci, 2008, 21(5):398-403.
9.	王懋, 張長青. 股骨頭缺血性壞死實驗動物模型:分類與實驗應用. 中國組織工程研究, 2014, 18(36):5879-5884.
10.	張志輝, 李玲, 劉曉倩, 等. 急性細菌脂多糖暴露對小鼠血糖的影響. 安徽醫科大學學報, 2013, 48(2):142-145.
11.	Powell C, Chang C, Gershwin ME. Current concepts on the pathogenesis and natural history of steroid-induced osteonecrosis. Clin Rev Allergy Immunol, 2011, 41(1):102-113.
12.	Yamaguchi R, Yamamoto T, Motomura G, et al. Effects of an antiplatelet drug on the prevention of steroid-induced osteonecrosis in rabbits. Rheumatology, 2012, 51(5):789-793.
13.	范猛, 汪愛媛, 王玉, 等. 局部冷熱交替損傷建立鴯鹋股骨頭缺血性壞死塌陷模型. 中國醫學科學院學報, 2011, 33(4):375-381.
14.	Zheng LZ, Liu Z, Lei M, et al. Steroid-associated hip joint collapse in bipedal emus. PLoS One, 2013, 8(10):e76797.
15.	鎖詠梅, 張雪君, 王植, 等. X 線與MRI評價股骨頭缺血性壞死分期的比較. 天津醫科大學學報, 2012, 18(4):484-487.
16.	Effendy NM, Khamis MF, Shuid AN. Micro-CT assessments of potential anti-osteoporotic agents. Curr Drug Targets, 2013, 14(13): 1542-1551.
17.	Yang L, Boyd K, Kaste SC, et al. A mouse model for glucocorticoid induced osteonecrosis:effect of a steroid holiday. J Orthop Res, 2009, 27(2):169-175.
18.	Wang W, Liu L, Dang X, et al. The effect of core decompression on local expression of BMP-2, PPAR-rand bone regeneration in the steroid-induced femoral head osteonecrosis. BMC Musculoskeletal Disorders, 2012, 13:142-150.
19.	董越, 王琰, 王紹武, 等. MR彌散加權成像診斷股骨頭缺血性壞死. 中國醫學影像技術, 2012, 28(2):352-355.
20.	李瑞琦, 張國平, 李宜炯, 等. 激素性股骨頭缺血性壞死模型:不同構建技術分析. 中國組織工程研究, 2013, 17(37):6676-6681.

1. Wang XS, Zhuang QY, Weng XS, et al. Etiological and clinical analysis of osteonecrosis of the femoral head in Chinese patients. Chin Med J (Engl), 2013, 126(2):290-295.
2. 周磊, 翁習生. 激素性股骨頭缺血性壞死動物模型的選擇與應用. 中國矯形外科雜志, 2013, 21(21):2155-2158.
3. Guan XY, Han D. Role of hypercoagulability in steroid-induced femoral head necrosis in rabbits. J Orthop Sci, 2010, 15(3):365-370.
4. 董玉雷, 周磊, 李玉龍, 等. 大鼠激素性股骨頭缺血性壞死模型的建立和評價. 中國醫學科學院學報, 2015, 37(2):152-156.
5. 張國橋, 易誠青, 滕松松, 等. 早期激素性股骨頭缺血性壞死模型的建立. 現代生物醫學進展, 2012, 12(7):1223-1224.
6. 李超, 尚希福, 李旭, 等. 提高激素性股骨頭缺血性壞死兔模型動物存活率的實驗研究. 安徽醫科大學學報, 2014, 49(12):1726-1729.
7. 楊帆, 朱振中, 李廣翼, 等. 股骨頭缺血性壞死病理形態學研究進展. 國際骨科學雜志, 2014, 35(5):313-315.
8. Wen Q, Ma L, Chen YP, et al. A rabbit model of hormone-induced early avascular necrosis of the femoral head. Biomed Environ Sci, 2008, 21(5):398-403.
9. 王懋, 張長青. 股骨頭缺血性壞死實驗動物模型:分類與實驗應用. 中國組織工程研究, 2014, 18(36):5879-5884.
10. 張志輝, 李玲, 劉曉倩, 等. 急性細菌脂多糖暴露對小鼠血糖的影響. 安徽醫科大學學報, 2013, 48(2):142-145.
11. Powell C, Chang C, Gershwin ME. Current concepts on the pathogenesis and natural history of steroid-induced osteonecrosis. Clin Rev Allergy Immunol, 2011, 41(1):102-113.
12. Yamaguchi R, Yamamoto T, Motomura G, et al. Effects of an antiplatelet drug on the prevention of steroid-induced osteonecrosis in rabbits. Rheumatology, 2012, 51(5):789-793.
13. 范猛, 汪愛媛, 王玉, 等. 局部冷熱交替損傷建立鴯鹋股骨頭缺血性壞死塌陷模型. 中國醫學科學院學報, 2011, 33(4):375-381.
14. Zheng LZ, Liu Z, Lei M, et al. Steroid-associated hip joint collapse in bipedal emus. PLoS One, 2013, 8(10):e76797.
15. 鎖詠梅, 張雪君, 王植, 等. X 線與MRI評價股骨頭缺血性壞死分期的比較. 天津醫科大學學報, 2012, 18(4):484-487.
16. Effendy NM, Khamis MF, Shuid AN. Micro-CT assessments of potential anti-osteoporotic agents. Curr Drug Targets, 2013, 14(13): 1542-1551.
17. Yang L, Boyd K, Kaste SC, et al. A mouse model for glucocorticoid induced osteonecrosis:effect of a steroid holiday. J Orthop Res, 2009, 27(2):169-175.
18. Wang W, Liu L, Dang X, et al. The effect of core decompression on local expression of BMP-2, PPAR-rand bone regeneration in the steroid-induced femoral head osteonecrosis. BMC Musculoskeletal Disorders, 2012, 13:142-150.
19. 董越, 王琰, 王紹武, 等. MR彌散加權成像診斷股骨頭缺血性壞死. 中國醫學影像技術, 2012, 28(2):352-355.
20. 李瑞琦, 張國平, 李宜炯, 等. 激素性股骨頭缺血性壞死模型:不同構建技術分析. 中國組織工程研究, 2013, 17(37):6676-6681.

Chinese Journal of Reparative and Reconstructive Surgery

MODEL ESTABLISHMENT, MRI AND PATHOLOGICAL FEATURES OF EARLY STEROID-INDUCED AVASCULAR NECROSIS OF FEMORAL HEAD IN RABBIT

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