EFFECT OF BONE MARROW MESENCHYMAL STEM CELLS ON GASTRIC ULCER REPAIRING_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

 WANGGuozhong , LIChengjun , FANXichao , LIBo , XIAOWei , JINLi

Department of Pathophysiology, Qiqihar Medical College, Qiqihar Heilongjiang, 161006, P. R. China;

Corresponding?author：

WANGGuozhong, Email: wgzbs@163.com

Keywords：

Bone marrow mesenchymal stem cells; Gastric ulcer; Vascular endothelial growth factor; Ulcer healing; Rat

DOI：

10.7507/1002-1892.20150191

Video：

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Objective To explore the effect and mechanisms of bone marrow mesenchymal stem cells (BMSCs) on healing quality of acetic acid-induced gastric ulcer. Methods Forty-eight clean grade male Wistar rats were used to establish the model of gastric ulcer with acetic acid and were randomly divided into 3 groups after 3 days of modeling, 16 rats each group. After the abdominal cavity was open and stomach was pulled out, no treatment was given in group A, 150 μL phosphate buffered saline (PBS) and 150 μL BMSCs at passage 4+PBS (1×108 cells/100 μL) were injected into the gastric wall surrounding the ulcer at 5 different points in groups B and C respectively. After 10 days, the ulcer area was measured, the mucosal thickness and the number of dilated glands were tested in the regenerative mucosa by histological method. And the expression of vascular endothelial growth factor (VEGF) was detected at ulcerative margin by immunohistochemical method. Results The ulcer area in group C was significantly smaller than that of groups A and B (P<0.01), but no significant difference was found between groups A and B (P>0.05). HE staining showed that group C had thicker regenerative gastric mucosa, less dilated glands, and more regular mucosal structure than groups A and B, showing significant differences in regenerative gastric mucosa thickness and dilated glands number (P<0.01), but no significant difference between groups A and B (P>0.05). Immunohistochemical staining showed that the positive expression of VEGF in the ulcer margin mucosa of group C was significantly higher than that of groups A and B. The integral absorbance (IA) value of VEGF expression in group C was significantly higher than that in groups A and B (P<0.01), but no significant difference between groups A and B (P>0.05). Conclusion BMSCs can accelerate ulcer healing by the secretion of VEGF, and improve the quality of ulcer healing.

Citation： WANGGuozhong, LIChengjun, FANXichao, LIBo, XIAOWei, JINLi. EFFECT OF BONE MARROW MESENCHYMAL STEM CELLS ON GASTRIC ULCER REPAIRING. Chinese Journal of Reparative and Reconstructive Surgery, 2015, 29(7): 889-892. doi: 10.7507/1002-1892.20150191 Copy

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2.	Hayashi Y,Tsuji S,Tsujii M,et al.Topical transplantation of mesenchymal stem cells accelerates gastric ulcer healing in rats.Am J Physiol Gastrointest Liver Physiol,2008,294(3):778-786.
3.	Nishida T,Tsuji S,Tsujii M,et al.Cultured bone marrow cell local implantation accelerates healing of ulcers in mice.J Gastroenterol,2008,43(2):124-135.
4.	劉韓,崔偉龍,牟林,等.阿司匹林促進胃酸分泌的途徑與潰瘍復發.中國醫藥導報,2010,7(34):16-18.
5.	王國忠,肖薇,李波.阿司匹林對大鼠醋酸胃潰瘍愈合的影響.中國醫院藥學雜志,2013,33(23):1925-1928.
6.	王國忠,夏立丁,崔偉龍,等.幽門螺桿菌與阿司匹林對潰瘍愈合的影響.中國全科醫學,2011,14(2C):586-588,591.
7.	Liu P,Feng Y,Wang Y,et al.Therapeutic action of bone marrow-derived stem cells against acute kidney injury.Life Sci,2014,115(1-2):1-7.
8.	王貴均,林麟孫,香鈺鴻.骨髓間充質干細胞治療腦出血的臨床觀察.現代預防醫學,2011,38(23):5018-5019.
9.	Hang HL,Xia Q.Role of BMSCs in liver regeneration and metastasis after hepatectomy.World J Gastroenterol,2014,20(1):126-132.
10.	王鋒,吳小濤,王運濤,等.干細胞移植修復椎間盤退變的研究現狀.中國修復重建外科雜志,2013,27(5):575-579.
11.	楊國強,范學慧,蕭洪文.骨髓間充質干細胞移植治療缺血性腦損傷的最新研究進展.四川解剖學雜志,2013,21(3):32-35.
12.	梁濤,劉浩.間充質干細胞移植治療椎間盤退變疾病的研究進展.中國修復重建外科雜志,2007,21(5):502-506.
13.	Kagami H,Agata H,Tojo A.Bone marrow stromal cells (bone marrow-derived multipotent mesenchymal stromal cells) for bone tissue engineering:basic science to clinical translation.Int J Biochem Cell Biol,2011,43(3):286-289.
14.	焦雪峰,黃永燦,黃益洲,等.大鼠BMSCs自發鈣化過程中成骨相關基因表達譜的分析.中國修復重建外科雜志,2014,28(2):133-141.
15.	韓冰,付小兵.間充質干細胞的研究進展與臨床應用前景.中國修復重建外科雜志,2006,20(12):1257-1261.
16.	李成軍,王麗萍,夏立丁,等.大鼠乙酸胃潰瘍愈合過程中EGF和VEGF對胃黏膜組織形態的影響.醫學研究雜志,2010,39(8):56-59.
17.	Liu Y,Dulchavsky DS,Gao X,et al.Wound repair by bone marrow stromal cells through growth factor production.J Surg Res,2006,136(2):336-341.
18.	Chang Q,Yan L,Wang CZ,et al.In vivo transplantation of bone marrow mesenchymal stem cells accelerates repair of injured gastric mucosa in rats.Chin Med J (Engl),2012,125(6):1169-1174.
19.	Askarov MB,Onischenko NA.Multipotent mesenchymal stromal cells of autologous bone marrow stimulate neoangiogenesis,restore microcirculation,and promote healing of indolent ulcers of the stomach.Bull Exp Biol Med,2008,146(4):512-516.
20.	晏開力,汪健,李慶,等.成人骨髓間充質干細胞分化為血管內皮細胞的研究.中國修復重建外科雜志,2007,21(1):76-80.
21.	張明珠,俞光榮.干細胞移植治療糖尿病足的基礎研究與臨床應用進展.中國修復重建外科雜志,2009,23(3):358-361.

1. Hoch AI,Leach JK.Concise review:optimizing expansion of bone marrow mesenchymal stem/stromal cells for clinical applications.Stem Cells Transl Med,2014,3(5):643-652.
2. Hayashi Y,Tsuji S,Tsujii M,et al.Topical transplantation of mesenchymal stem cells accelerates gastric ulcer healing in rats.Am J Physiol Gastrointest Liver Physiol,2008,294(3):778-786.
3. Nishida T,Tsuji S,Tsujii M,et al.Cultured bone marrow cell local implantation accelerates healing of ulcers in mice.J Gastroenterol,2008,43(2):124-135.
4. 劉韓,崔偉龍,牟林,等.阿司匹林促進胃酸分泌的途徑與潰瘍復發.中國醫藥導報,2010,7(34):16-18.
5. 王國忠,肖薇,李波.阿司匹林對大鼠醋酸胃潰瘍愈合的影響.中國醫院藥學雜志,2013,33(23):1925-1928.
6. 王國忠,夏立丁,崔偉龍,等.幽門螺桿菌與阿司匹林對潰瘍愈合的影響.中國全科醫學,2011,14(2C):586-588,591.
7. Liu P,Feng Y,Wang Y,et al.Therapeutic action of bone marrow-derived stem cells against acute kidney injury.Life Sci,2014,115(1-2):1-7.
8. 王貴均,林麟孫,香鈺鴻.骨髓間充質干細胞治療腦出血的臨床觀察.現代預防醫學,2011,38(23):5018-5019.
9. Hang HL,Xia Q.Role of BMSCs in liver regeneration and metastasis after hepatectomy.World J Gastroenterol,2014,20(1):126-132.
10. 王鋒,吳小濤,王運濤,等.干細胞移植修復椎間盤退變的研究現狀.中國修復重建外科雜志,2013,27(5):575-579.
11. 楊國強,范學慧,蕭洪文.骨髓間充質干細胞移植治療缺血性腦損傷的最新研究進展.四川解剖學雜志,2013,21(3):32-35.
12. 梁濤,劉浩.間充質干細胞移植治療椎間盤退變疾病的研究進展.中國修復重建外科雜志,2007,21(5):502-506.
13. Kagami H,Agata H,Tojo A.Bone marrow stromal cells (bone marrow-derived multipotent mesenchymal stromal cells) for bone tissue engineering:basic science to clinical translation.Int J Biochem Cell Biol,2011,43(3):286-289.
14. 焦雪峰,黃永燦,黃益洲,等.大鼠BMSCs自發鈣化過程中成骨相關基因表達譜的分析.中國修復重建外科雜志,2014,28(2):133-141.
15. 韓冰,付小兵.間充質干細胞的研究進展與臨床應用前景.中國修復重建外科雜志,2006,20(12):1257-1261.
16. 李成軍,王麗萍,夏立丁,等.大鼠乙酸胃潰瘍愈合過程中EGF和VEGF對胃黏膜組織形態的影響.醫學研究雜志,2010,39(8):56-59.
17. Liu Y,Dulchavsky DS,Gao X,et al.Wound repair by bone marrow stromal cells through growth factor production.J Surg Res,2006,136(2):336-341.
18. Chang Q,Yan L,Wang CZ,et al.In vivo transplantation of bone marrow mesenchymal stem cells accelerates repair of injured gastric mucosa in rats.Chin Med J (Engl),2012,125(6):1169-1174.
19. Askarov MB,Onischenko NA.Multipotent mesenchymal stromal cells of autologous bone marrow stimulate neoangiogenesis,restore microcirculation,and promote healing of indolent ulcers of the stomach.Bull Exp Biol Med,2008,146(4):512-516.
20. 晏開力,汪健,李慶,等.成人骨髓間充質干細胞分化為血管內皮細胞的研究.中國修復重建外科雜志,2007,21(1):76-80.
21. 張明珠,俞光榮.干細胞移植治療糖尿病足的基礎研究與臨床應用進展.中國修復重建外科雜志,2009,23(3):358-361.

Chinese Journal of Reparative and Reconstructive Surgery

EFFECT OF BONE MARROW MESENCHYMAL STEM CELLS ON GASTRIC ULCER REPAIRING

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