EFFECTS OF LEUCOCYTE- AND PLATELET-RICH PLASMA ON OSTEOGENIC DIFFERENTIATION OF BONE MARROW MESENCHYMAL STEM CELLS IN TREATING AVASCULAR NECROSIS OF FEMORAL HEAD IN RABBITS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

FANGJialiu ,  YINZongsheng , WANGWei , LUMing , JIANGZheng , GAOWeilu , ZhangShuo , YuTao

Department of Orthopaedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine;
1. Institute of Basic Medicine, Shandong Academy of Medical Sciences;

Corresponding?author：

YINZongsheng, Email: yinzongsheng@sina.com

Keywords：

Bone marrow mesenchymal stem cells; Leucocyte-and platelet-rich plasma; Avascular necrosis of femoral head; Osteogenic differentiation; Rabbit

DOI：

10.7507/1002-1892.20150048

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To evaluate the effect of leucocyte- and platelet-rich plasma (L-PRP) on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in treating avascular necrosis of the femoral head (ANFH) in rabbits. Methods Twenty-four New Zealand white rabbits (4-6 months old, both genders, weighing 2.0-3.0 kg) were used for the establishment of bilateral ANFH models and divided into 4 groups (n=6). BMSCs were isolated from the bone marrow of iliac crest, cultured and identified. L-PRP was prepared by Landesberg method. Core decompression only (group A), core decompression and L-PRP implantation (group B), core decompression and BMSCs implantation (group C), and core decompression and implantation of BMSCs and L-PRP were performed in 4 groups. To evaluate bone formation and remodeling of the defects, X-ray photography was taken at 2, 4, and 8 weeks postoperatively. The modified Lane-Sandhu scoring system was used to evaluate the bone formation. Two rabbits were sacrificed at 2, 4, 8 weeks after operation to harvest the specimens for histological observation, new blood vessel count and new bone area ratio. Results The observations of radiology and histology displayed different degrees of bone regeneration at bone defect sites in each group. At 2, 4, and 8 weeks postoperatively, the results of Lane-Sandhu X-ray photography scoring, new blood vessel count, and new bone area ratio showed that groups C and D were significantly better than groups A and B, group D was significantly better than group C. and group B was significantly better than group A (P<0.05). Conclusion These findings demonstrate that L-PRP can promote osteogenic differentiation of BMSCs in treating ANFH in rabbits, and core decompression associated with BMSCs and L-PRP is an effective and feasible method to treat ANFH.

Citation： FANGJialiu, YINZongsheng, WANGWei, LUMing, JIANGZheng, GAOWeilu, ZhangShuo, YuTao. EFFECTS OF LEUCOCYTE- AND PLATELET-RICH PLASMA ON OSTEOGENIC DIFFERENTIATION OF BONE MARROW MESENCHYMAL STEM CELLS IN TREATING AVASCULAR NECROSIS OF FEMORAL HEAD IN RABBITS. Chinese Journal of Reparative and Reconstructive Surgery, 2015, 29(2): 227-233. doi: 10.7507/1002-1892.20150048 Copy

1.	徐俊, 張長青, 孫建琪, 等. 計算機輔助下低溫手術建立三足犬股骨頭壞死模型的初步報告. 中國修復重建外科雜志, 2008, 22(3):276-280.
2.	Babhulkar S. Osteonecrosis of femoral head:treatment by core decompression and vascular pedicle grafting. Indian J Orthop, 2009, 43(1):27-35.
3.	曹亞偉, 王義生, 韓楓, 等. 骨髓間充質干細胞和傘狀支撐骨移植術治療股骨頭壞死的實驗研究. 中華顯微外科雜志, 2010, 33(4):293-296.
4.	Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science, 1999, 284(5411):143-147.
5.	Wang BL, Sun W, Shi ZC, et al. Treatment of nontraumatic osteonecrosis of the femoral head with the implantation of core decompression and concentrated autologous bone marrow containing mononuclear cells. Arch Orthop Trauma Surg, 2010, 130(7):859-865.
6.	謝雪濤, 施忠民, 張長青. 富血小板血漿的制備與臨床應用研究進展. 中華創傷骨科雜志, 2014, 16(4):311-315.
7.	Dallari D, Fini M, Stagni C, et al. In vitro study on the healing of bone defects treated with bone marrow stromal cells, platelet-rich plasma, and freeze-dried bone allografts, alone and in combination. J Orthop Res, 2006, 24(5):877-888.
8.	Arpornmaeklong P, Kochel M, Depprich R, et al. Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells. An in vitro study. Int J Oral Maxillofac Surg, 2004, 33(1):60-70.
9.	Ogino Y, Ayukawa Y, Tsukiyama Y, et al. The effect of platelet-rich plasma on the cellular response of rat bone marrow cells in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005, 100(3):302-307.
10.	Landsberg R, Roy M, Glickman RS. Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation. J Oral Maxillofac Surg, 2000, 58(3):297-300.
11.	孫偉, 李子榮, 史振才, 等. 納米晶膠原基骨和骨髓間充質干細胞復合修復兔股骨頭壞死缺損的研究. 中國修復重建外科雜志, 2005, 19(9):703-706.
12.	Lane JM, Sandhu HS. Current approaches to experimental bone grafting. Orthop Clin North (Am), 1987, 18(2):213-225.
13.	李子榮. 科學診斷和治療股骨頭壞死. 中國修復重建外科雜, 2005, 19(9):685-686.
14.	Castro FP Jr, Barrack RL. Core decompression and conservative treatment for avascular necrosis of the femoral head:a meta-analysis. Am J Orthop (Belle Mead NJ), 2000, 29(3):187-194.
15.	Buckley PD, Gearen PF, Petty RW. Structural bone grafting for early atraumatic avascular necrosis of the femoral head. J Bone Joint Surg (Am), 1991, 73(9):1357-1364.
16.	Steinberg ME, Larcom PG, Strafford B, et al. Core decompression with bone grafting for osteonecrosis of the femoral head. Clin Orthop Relat Res, 2001, (386):71-78.
17.	Lieberman JR. Core decompression for osteonecrosis of the hip. Clin Orthop Relat Res, 2004, (418):29-33.
18.	Israelite C, Nelson CL, Ziarani CF, et al. Bilateral core decompression for osteonecrosis of the femoral head. Clin Orthop Relat Res, 2005, (441):285-290.
19.	Hernigou P, Beaujean F, Lambotte JC. Decrease of mesenchymal stem cell pool in the upper femoral extremity of patients with osteonecrosis related to corticosteroidtherapy. J Bone Joint Surg (Br), 1999, 81(2):349-355.
20.	Wang BL, Sun W, Shi ZC, et al. Decreased Proliferation of Mesenchymal Stem Cells in Corticosteroid-induced Osteonecrosis of Femoral Head. Orthopedics, 2008, 31(5):444.
21.	Kanno T, Takahashi T, Tsujisawa T, et al. Platelet-rich plasma enhances human osteoblast-like cell proliferation and differentiation. J Oral Maxillofac Surg, 2005, 63(3):362-369.
22.	Oryan A, Meimandi Parizi A, Shafiei-Sarvestani Z, et al. Effects of combined hydroxyapatite and human platelet rich plasma on bone healing in rabbit model:radiological,macroscopical, hidtopathological and biomechanical evaluation. Cell Tissue Bank, 2012, 13(4):639-651.
23.	Aghaloo TL, Moy PK, Freymiller EG. Investigation of platelet-plasma in rabbit cranial defects:a pilot study. J Oral Maxillofac Surg, 2002, 60(10):1176-1181.
24.	Schlegel KA, Donath K, Rupprecht S, et al. De novo bone formation using bovine collagen and platelet-rich plasma. Biomaterials, 2004, 25(23):5387-5393.
25.	Chen L, Yang X, Huang G, et al. Platelet-rich Plasma Promotes Healing of Osteoporotic Fractures. Orthopedics, 2013, 36(6):e687-694.
26.	Thorwarth M, Rupprecht S, Falk S, et al. Expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (prp)-an immunohistochemical analysis. Biomaterials, 2005, 26(15):2575-2584.

1. 徐俊, 張長青, 孫建琪, 等. 計算機輔助下低溫手術建立三足犬股骨頭壞死模型的初步報告. 中國修復重建外科雜志, 2008, 22(3):276-280.
2. Babhulkar S. Osteonecrosis of femoral head:treatment by core decompression and vascular pedicle grafting. Indian J Orthop, 2009, 43(1):27-35.
3. 曹亞偉, 王義生, 韓楓, 等. 骨髓間充質干細胞和傘狀支撐骨移植術治療股骨頭壞死的實驗研究. 中華顯微外科雜志, 2010, 33(4):293-296.
4. Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science, 1999, 284(5411):143-147.
5. Wang BL, Sun W, Shi ZC, et al. Treatment of nontraumatic osteonecrosis of the femoral head with the implantation of core decompression and concentrated autologous bone marrow containing mononuclear cells. Arch Orthop Trauma Surg, 2010, 130(7):859-865.
6. 謝雪濤, 施忠民, 張長青. 富血小板血漿的制備與臨床應用研究進展. 中華創傷骨科雜志, 2014, 16(4):311-315.
7. Dallari D, Fini M, Stagni C, et al. In vitro study on the healing of bone defects treated with bone marrow stromal cells, platelet-rich plasma, and freeze-dried bone allografts, alone and in combination. J Orthop Res, 2006, 24(5):877-888.
8. Arpornmaeklong P, Kochel M, Depprich R, et al. Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells. An in vitro study. Int J Oral Maxillofac Surg, 2004, 33(1):60-70.
9. Ogino Y, Ayukawa Y, Tsukiyama Y, et al. The effect of platelet-rich plasma on the cellular response of rat bone marrow cells in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005, 100(3):302-307.
10. Landsberg R, Roy M, Glickman RS. Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation. J Oral Maxillofac Surg, 2000, 58(3):297-300.
11. 孫偉, 李子榮, 史振才, 等. 納米晶膠原基骨和骨髓間充質干細胞復合修復兔股骨頭壞死缺損的研究. 中國修復重建外科雜志, 2005, 19(9):703-706.
12. Lane JM, Sandhu HS. Current approaches to experimental bone grafting. Orthop Clin North (Am), 1987, 18(2):213-225.
13. 李子榮. 科學診斷和治療股骨頭壞死. 中國修復重建外科雜, 2005, 19(9):685-686.
14. Castro FP Jr, Barrack RL. Core decompression and conservative treatment for avascular necrosis of the femoral head:a meta-analysis. Am J Orthop (Belle Mead NJ), 2000, 29(3):187-194.
15. Buckley PD, Gearen PF, Petty RW. Structural bone grafting for early atraumatic avascular necrosis of the femoral head. J Bone Joint Surg (Am), 1991, 73(9):1357-1364.
16. Steinberg ME, Larcom PG, Strafford B, et al. Core decompression with bone grafting for osteonecrosis of the femoral head. Clin Orthop Relat Res, 2001, (386):71-78.
17. Lieberman JR. Core decompression for osteonecrosis of the hip. Clin Orthop Relat Res, 2004, (418):29-33.
18. Israelite C, Nelson CL, Ziarani CF, et al. Bilateral core decompression for osteonecrosis of the femoral head. Clin Orthop Relat Res, 2005, (441):285-290.
19. Hernigou P, Beaujean F, Lambotte JC. Decrease of mesenchymal stem cell pool in the upper femoral extremity of patients with osteonecrosis related to corticosteroidtherapy. J Bone Joint Surg (Br), 1999, 81(2):349-355.
20. Wang BL, Sun W, Shi ZC, et al. Decreased Proliferation of Mesenchymal Stem Cells in Corticosteroid-induced Osteonecrosis of Femoral Head. Orthopedics, 2008, 31(5):444.
21. Kanno T, Takahashi T, Tsujisawa T, et al. Platelet-rich plasma enhances human osteoblast-like cell proliferation and differentiation. J Oral Maxillofac Surg, 2005, 63(3):362-369.
22. Oryan A, Meimandi Parizi A, Shafiei-Sarvestani Z, et al. Effects of combined hydroxyapatite and human platelet rich plasma on bone healing in rabbit model:radiological,macroscopical, hidtopathological and biomechanical evaluation. Cell Tissue Bank, 2012, 13(4):639-651.
23. Aghaloo TL, Moy PK, Freymiller EG. Investigation of platelet-plasma in rabbit cranial defects:a pilot study. J Oral Maxillofac Surg, 2002, 60(10):1176-1181.
24. Schlegel KA, Donath K, Rupprecht S, et al. De novo bone formation using bovine collagen and platelet-rich plasma. Biomaterials, 2004, 25(23):5387-5393.
25. Chen L, Yang X, Huang G, et al. Platelet-rich Plasma Promotes Healing of Osteoporotic Fractures. Orthopedics, 2013, 36(6):e687-694.
26. Thorwarth M, Rupprecht S, Falk S, et al. Expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (prp)-an immunohistochemical analysis. Biomaterials, 2005, 26(15):2575-2584.

Chinese Journal of Reparative and Reconstructive Surgery

EFFECTS OF LEUCOCYTE- AND PLATELET-RICH PLASMA ON OSTEOGENIC DIFFERENTIATION OF BONE MARROW MESENCHYMAL STEM CELLS IN TREATING AVASCULAR NECROSIS OF FEMORAL HEAD IN RABBITS

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content