EXPRESSIONS OF OSTEOGENESIS RELATED FACTORS IN MG63 CELLS CO-CULTURED WITH DOMESTIC POROUS TANTALUM_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

WANGQian ^1,2 , ZHANGHui ^1,3 , GENGLixin ⁴ , LIQijia ⁴ , GANHongquan ⁵ , WANGHui ⁶ , BICheng ⁵ ,  WANGZhiqiang ^1,5

1. 唐山市第二醫院手一科;
2. Graduate School, Southern Medical University, Guangzhou Guangdong, 510515, P. R. China;
3. Department of Anatomy, Basic Medical College;
4. the First Department of Joint Surgery, 6the First Department of Hand Surgery, Tangshan No. 2 Hospital;
5. Experimental Center, Hebei United University;
6. Department of Orthopaedics, Affiliated Hospital of Hebei United University;

Corresponding?author：

WANGZhiqiang, Email: wzhqde@163.com

Keywords：

Domestic porous tantalum; MG63 cells; Runt-related transcri ption factor 2; Osteocalcin; Fibronectin; Bone tissue engineering

DOI：

10.7507/1002-1892.20140306

Video：

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Objective To investigate the feasibil ity of the domestic porous tantalum as scaffold material of bone tissue engineering by observing the expressions of osteogenesis related factors of MG63 cells co-cultured with domestic porous tantalum. Methods MG63 cells were cultured with porous tantalum scaffolds (group A), with porous tantalum leaching solution (group B), and with MEM as control group (group C). The cell adhesion of group A was observed on the scaffolds at 3, 5, and 7 days after culture by scanning electron microscopy (SEM); immunohistochemistry and Western blot methods were used to detect the expressions of Runt-related transcri ption factor 2 (Runx-2), osteocalcin (OC), and fibronectin (FN). Results At 3 days after culture, the cells of group A adhered the surface and pore of the porous tantalum scaffolds, with sparse cell arrangement and less protuberances; at 5 days after culture, adjacent cells connected to be a flat each other, which covered the surface and pore of the scaffold; at 7 days after culture, cells secreted plenty of extracellular matrix, covering most of the material surface. The expressions of Runx-2, OC, and FN were positive in 3 groups; darker staining of the cytoplasm was observed in group A, the expressions were significantly higher in group A than in other 2 groups. The results of immunohistochemistry and Western blot showed that the expressions of Runx-2 and OC were significantly increased in group A when compared with those in groups B and C (P < 0.05), but no significant difference was found between groups B and C (P > 0.05). The expression of FN had no significant difference among 3 groups (P > 0.05). Conclusion Domestic porous tantalum could promote MG63 cells adhesion and growth, and may promote the expressions of Runx-2 and OC, so it can be used as a scaffold material of bone tissue engineering.

Citation： WANGQian, ZHANGHui, GENGLixin, LIQijia, GANHongquan, WANGHui, BICheng, WANGZhiqiang. EXPRESSIONS OF OSTEOGENESIS RELATED FACTORS IN MG63 CELLS CO-CULTURED WITH DOMESTIC POROUS TANTALUM. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(11): 1422-1427. doi: 10.7507/1002-1892.20140306 Copy

1.	Mrosek EH, Schagemann JC, Chung HW, et al. Porous tantalum and poly-epsilon-caprolactone biocomposites for osteochondral defect repair:preliminary studies in rabbits. J Orthop Res, 2010, 28(2):141-148.
2.	朱留洋, 李琪佳.金屬鉭在骨損傷康復治療中的應用.中國矯形外科雜志, 2013, 21(6):578-581.
3.	Fernandez-Fairen M, Querales V, Jakowlew A, et al. Tantalum is a good bone graft substitute in tibial tubercle advancement. Clin Orthop Relat Res, 2010, 468(5):1284-1295.
4.	Wang N, Li H, Wang J, et al. Study on the anticorrosion, biocompatibil ity, and osteoin-ductivity of tantalum decorated with tantalum oxide nanotube array films. ACS Appl Mater Interfaces, 2012, 4(9):4516-4523.
5.	甘洪全, 劉鑫, 李琪佳.醫學領域的新型骨科植入材料-多孔鉭.中國煤炭工業醫學雜志, 2014, 17(6):1023-1026.
6.	王大鵬, 李琪佳, 王志強.醫用多孔鉭的性能及其在膝關節損傷中的應用.中國組織工程研究與臨床康復, 2011, 15(29):5463-5466.
7.	甘洪全, 劉鑫, 李琪佳.醫用多孔金屬鉭材料的基礎研究與應用.中外醫療, 2014, (14):191-192.
8.	李慶慶, 黃江鴻, 何美健, 等. Runx-2及其在骨組織工程中的應用.國際骨科學雜志, 2014, 35(1):44-46.
9.	張蒼宇, 汪新柱, 任廣鐵, 等.組織工程化骨膜的構建及其在體外的成骨活性檢測.中國矯形外科雜志, 2014, 22(3):256-261.
10.	王福科, 劉流, 代曉明, 等.纖維粘連蛋白對部分脫蛋白骨細胞相容性影響研究.昆明醫學院學報, 2010, (1):9-14.
11.	耿麗鑫, 甘洪全, 王茜, 等.國產多孔鉭對成骨細胞生物相容性及其相關成骨基因表達的影響.第三軍醫大學學報, 2014, 36(11):1163-1167.
12.	中華人民共和國國家標準. GB/TISO10993-5:2009醫療器械生物學評價.北京:中國標準出版社, 2009.
13.	熊建義, 歐陽建安, 王大平, 等.多孔鈮基生物材料的制備及其性能研究.中國臨床解剖學雜志, 2013, 31(1):89-94.
14.	馬劍, 李乾, 尹蕓生.鉭金屬在醫學領域的應用.實用骨科雜志, 2014, 20(4):329-333.
15.	Sagomonyants KB, Hakim-Zargar M, Jhaveri A, et al. Porous tantalum stimulates the prol iferation and osteogenesis of osteoblasts from elderly female patients. J Orthop Res, 2011, 29(4):609-616.
16.	李慕勤, 王健平, 趙莉.天然高分子/羥基磷灰石支架材料對成骨細胞增殖的影響.黑龍江醫藥科學, 2008, 31(5):1-2.
17.	Fox P, Pogson S, Sutcliffe CJ, et al. Interface interactions between porous titanium/tantalum coatings, produced by Selective Laser Melting (SLM), on a cobalt-chromium alloy. Surface Coatings Techonlogy, 2008, 202(20):5001-5007.
18.	林鳳飛, 鄭明, 劉伯齡, 等.多孔鉭金屬與成骨細胞生物相容性的實驗研究.中國矯形外科雜志, 2014, 22(12):1119-1123.
19.	趙艷芳, 宋濤, 劉躍亮, 等. RUNX2對BMP9誘導的間充質干細胞C3H10T1/2成骨分化的影響.中國生物工程雜志, 2013, 33(2):21-26.
20.	Hoang QQ, Sicheri F, Howard AJ, et al. Bone recognition mechanism of porcine osteocalcin from crystal structure. Nature, 2003, 425(6961): 977-980.
21.	韓濤, 丁佑銘.骨橋蛋白基本特性及在疾病中的作用的研究進展.實用醫藥雜志, 2011, 28(6):554-556.

1. Mrosek EH, Schagemann JC, Chung HW, et al. Porous tantalum and poly-epsilon-caprolactone biocomposites for osteochondral defect repair:preliminary studies in rabbits. J Orthop Res, 2010, 28(2):141-148.
2. 朱留洋, 李琪佳.金屬鉭在骨損傷康復治療中的應用.中國矯形外科雜志, 2013, 21(6):578-581.
3. Fernandez-Fairen M, Querales V, Jakowlew A, et al. Tantalum is a good bone graft substitute in tibial tubercle advancement. Clin Orthop Relat Res, 2010, 468(5):1284-1295.
4. Wang N, Li H, Wang J, et al. Study on the anticorrosion, biocompatibil ity, and osteoin-ductivity of tantalum decorated with tantalum oxide nanotube array films. ACS Appl Mater Interfaces, 2012, 4(9):4516-4523.
5. 甘洪全, 劉鑫, 李琪佳.醫學領域的新型骨科植入材料-多孔鉭.中國煤炭工業醫學雜志, 2014, 17(6):1023-1026.
6. 王大鵬, 李琪佳, 王志強.醫用多孔鉭的性能及其在膝關節損傷中的應用.中國組織工程研究與臨床康復, 2011, 15(29):5463-5466.
7. 甘洪全, 劉鑫, 李琪佳.醫用多孔金屬鉭材料的基礎研究與應用.中外醫療, 2014, (14):191-192.
8. 李慶慶, 黃江鴻, 何美健, 等. Runx-2及其在骨組織工程中的應用.國際骨科學雜志, 2014, 35(1):44-46.
9. 張蒼宇, 汪新柱, 任廣鐵, 等.組織工程化骨膜的構建及其在體外的成骨活性檢測.中國矯形外科雜志, 2014, 22(3):256-261.
10. 王福科, 劉流, 代曉明, 等.纖維粘連蛋白對部分脫蛋白骨細胞相容性影響研究.昆明醫學院學報, 2010, (1):9-14.
11. 耿麗鑫, 甘洪全, 王茜, 等.國產多孔鉭對成骨細胞生物相容性及其相關成骨基因表達的影響.第三軍醫大學學報, 2014, 36(11):1163-1167.
12. 中華人民共和國國家標準. GB/TISO10993-5:2009醫療器械生物學評價.北京:中國標準出版社, 2009.
13. 熊建義, 歐陽建安, 王大平, 等.多孔鈮基生物材料的制備及其性能研究.中國臨床解剖學雜志, 2013, 31(1):89-94.
14. 馬劍, 李乾, 尹蕓生.鉭金屬在醫學領域的應用.實用骨科雜志, 2014, 20(4):329-333.
15. Sagomonyants KB, Hakim-Zargar M, Jhaveri A, et al. Porous tantalum stimulates the prol iferation and osteogenesis of osteoblasts from elderly female patients. J Orthop Res, 2011, 29(4):609-616.
16. 李慕勤, 王健平, 趙莉.天然高分子/羥基磷灰石支架材料對成骨細胞增殖的影響.黑龍江醫藥科學, 2008, 31(5):1-2.
17. Fox P, Pogson S, Sutcliffe CJ, et al. Interface interactions between porous titanium/tantalum coatings, produced by Selective Laser Melting (SLM), on a cobalt-chromium alloy. Surface Coatings Techonlogy, 2008, 202(20):5001-5007.
18. 林鳳飛, 鄭明, 劉伯齡, 等.多孔鉭金屬與成骨細胞生物相容性的實驗研究.中國矯形外科雜志, 2014, 22(12):1119-1123.
19. 趙艷芳, 宋濤, 劉躍亮, 等. RUNX2對BMP9誘導的間充質干細胞C3H10T1/2成骨分化的影響.中國生物工程雜志, 2013, 33(2):21-26.
20. Hoang QQ, Sicheri F, Howard AJ, et al. Bone recognition mechanism of porcine osteocalcin from crystal structure. Nature, 2003, 425(6961): 977-980.
21. 韓濤, 丁佑銘.骨橋蛋白基本特性及在疾病中的作用的研究進展.實用醫藥雜志, 2011, 28(6):554-556.

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Chinese Journal of Reparative and Reconstructive Surgery

EXPRESSIONS OF OSTEOGENESIS RELATED FACTORS IN MG63 CELLS CO-CULTURED WITH DOMESTIC POROUS TANTALUM

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