PROTEOMICS STUDY ON EFFECT OF BASIC FIBROBLAST GROWTH FACTOR LONG CIRCULATION LIPOSOME ON SPINAL CORD TRACTION INJURY IN RATS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

WANGWenyue ¹ , YANGTianfu ² ,  LIULei ² , PEIFuxing ²

1. Department of Orthopaedics, Guang'anmen Hospital, Chinese Academy of Medicine Science, Beijing, 100053, P. R. China;
2. Department of Orthopaedics, West China Hospital, Sichuan University;

Corresponding?author：

LIULei, Email: liuinsistence@163.com

Keywords：

Spinal cord traction injury; Proteomics; Basic fibroblast growth factor; Long circulation liposome; Nano ultra-high performance liquid chromatography-electrospray tandem mass spectrometry; Rat

DOI：

10.7507/1002-1892.20140303

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To explore the possible active mechanism of the basic fibroblast growth factor (bFGF) long circulation l iposome (LCL) (bFGF+LCL) on spinal cord traction injury in rats at the level of proteomics. Methods Twenty Sprague Dawly rats were randomly divided into groups A and B, 10 rats in each group. The models of spinal cord traction injury was established at T12-L3 spines. The rats were not treated in group A, and the rats were treated with bFGF+LCL (20μg/ kg) in group B. At 3 weeks after operation, the rats were sacrificed for harvesting T13-L2 spinal tissue specimens. The protein was extracted and quantified in the spinal tissue firstly. The proteins from spinal tissue were separated by two-dimensional gel electrophoresis and identified by mass spectrometry. The different expression profiling was established in each group, and the differentially expressed protein was determined by comparing the level of each spot with gel imaging software and manually. The proteins were identified by nano ultra-high performance liquid chromatography-electrospray tandem mass spectrometry (NanoUPLC-ESI-MS/MS), and the proteins were classified. Results The differentially expressed protein spots were found in 2 groups. Compared with group A, 4 spots were up-regulated and 6 were down-regulated in group B. NanoUPLC-ESI-MS/MS results showed that 18 significant proteins were identified in 26 differentially expressed proteins, including 4 apoptosis-related proteins, 3 nerve signal transduction related proteins, 7 proteins involved in metabolism, 1 unknown function protein, and 3 unnamed proteins. Conclusion The differentially expressed proteins are found in spinal cord traction injury of rats treated with bFGF+LCL. bFGF+LCL can affect the proteins expression in rats with spinal cord traction injury. The possible active mechanism is that it has protective and repair effects on injured spinal cord by nerve signal transduction, and regulation of nerve cells apoptosis and metabolism.

Citation： WANGWenyue, YANGTianfu, LIULei, PEIFuxing. PROTEOMICS STUDY ON EFFECT OF BASIC FIBROBLAST GROWTH FACTOR LONG CIRCULATION LIPOSOME ON SPINAL CORD TRACTION INJURY IN RATS. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(11): 1397-1405. doi: 10.7507/1002-1892.20140303 Copy

1.	Mugabe C, Azghani AO, Omri A. Li posome-mediated gentamicin delivery:development and activity against resistant strains of Pseudomonas aeruginosa isolated from cystic fibrosis patients. J Antimicrob-Chemother, 2005, 55(2):269-271.
2.	王鴻麗.急性脊髓損傷及電針治療大鼠的比較蛋白質組研究.北京:中國人民解放軍軍事醫學科學院, 2009.
3.	黃亞東, 項琪, 張宏波, 等.堿性成纖維細胞生長因子脂質體的制備和評價.中國藥學雜志, 2008, 43(13):992-997.
4.	Dos Santos N, Allen C, Doppen AM, et al. Influence of poly(ethylene glycol)grafting density and polymer length on liposomes:relating plasma circulation lifetimes to protein binding. Biochim Biophys Acta, 2007, 1768(6):1367-1377.
5.	王文岳, 楊天府, 劉雷, 等.一種新型脊柱撐開器的研制及脊髓牽張性損傷動物模型的建立.中國修復重建外科雜志, 2011, 25(6):705-710.
6.	Yaksh TL, Stevens CW. Simple catheter preparation for permitting bolus intrathecal administration during chronic intrathecal infusion. Pharmacol Biochem Behav, 1986, 25(2):483-485.
7.	Fisher M, Meadows ME, Tuyen D, et al. Delayed treatment with intravenous basic fibroblast growth factor reduces infarct size following permanent focal cerebral ischemia in rats. J Cereb FlowMetab, 1995, 15(6):953-959.
8.	Ramagli LS. Quantifying protein in 2-D PAGE solubil ization buffers. Methods Mol Biol, 1999, 112:99-103.
9.	Crommelin DJA, Daemen T, Scherphof GL, et al. Liposomes:vehicles for the targeted and controlled del ivery of peptides and proteins. J Control Release, 1997, 46(122):165-175.
10.	Pan W, Kastin AJ. Penetration of neurotrophins and cytoltines across the blood-brain/blood-spinal cord barrier. Adv Drug Deliv Rev, 1999, 36(2-3):291-298.
11.	王文岳, 楊天府, 劉雷, 等.大鼠急性脊髓牽張損傷相關蛋白質的篩選和鑒定.中華外科雜志, 2012, 50(8):737-743.
12.	De Nicola AF, Gonzalez SL, Labombarda F, et al. Progesterone treatment of spinal cord injury. J Mol Neurosci, 2006, 28(1):3-15.
13.	沈正祥, 呂紅斌, 李小明, 等.川芎嗪對大鼠急性脊髓損傷模型caspase-3和NF表達的影響.中南大學學報:醫學版, 2008, 33(8):693-699.
14.	謝玲林, 胡火珍, 楊明理, 等.成年大鼠急性脊髓全橫斷損傷后差異表達蛋白的蛋白組學研究.四川大學學報:自然科學版, 2008, 45(2): 419-425.
15.	Dimlich RV, Showers MJ, Shi pley MT. Densitometric analysis of cytochrome oxidase in ischemic rat brain. Brain Res, 1990, 516(2):181-191.
16.	譚小玲, 柳君澤, 曹利飛, 等.長時間缺氧對大鼠腦皮質線粒體細胞色素氧化酶活性及亞基Ⅰ、Ⅳ蛋白表達的影響.中華航空航天醫學雜志, 2002, 13(4):243-246.
17.	Nakatsuka H, Ohta S, Tanaka J, et al. Histochemical cytochrome coxidase activity and caspase-3 in gerbil hippocampal CA1 neurons after transient forebrain ischemia. Neurosci Lett, 2000, 285(2):127-130.
18.	Soane L, Kahraman S, Kristian T, et al. Mechanisms of impaired mitochondrial energy metabolism in acute and chronic neurodegenerative disorders. J Neurosci Res, 2007, 85(15):3407-3415.
19.	Ball RL, Albrecht T, Thompson WC, et al. Thrombin, EGF and phorbol myristate acetate stimulate tubul in polymerization in quiescent cells:A potential link to mitogenesis. Cell Motil Cytoskeleton, 1992, 23(4):265-278.
20.	Johnsen JI, Aurelio ON, Kwaja Z, et al. p53-mediated negative regulation of stathmin/Op18 expression is associated with G(2)/M cell-cycle arrest. Int J Cancer, 2000, 88(5):685-691.
21.	Manceau V, Gavet O, Curmi P, et al. Stathmin interaction with HSC70 family proteins. Electrophoresis, 1999, 20(2):409-417.
22.	Poulsen K, Bahl JM, Simonsen AH. Distinct transthyretin oxidation isoform profile in spinal fluid from patients with Alzheimer's disease and mild cognitive impairment. Clin Proteomics, 2014, 11(1):12.
23.	任惠民, 周志剛, 陳向軍, 等.重癥肌無力患者骨骼肌肉蛋白成分分析.中華神經科雜志, 2000, 33(4):227-230.
24.	Cabiscol E, Levine RL. The phosphatase activity of carbonic anhydraseⅢis reversibly regulated by glutathiolation. Proc Natl Acad Sic U S A, 1996, 93(9):4170-4174.
25.	Liu FT, Rabinovich GA. Galectins:regulators of acute and chronic inflammation. Ann N Y Acad Sci, 2010, 1183:158-182.
26.	Mendez-Huergo SP, Maller SM, Farez MF, et al. Integration of lectinglycan recognition systems and immune cell networks in CNS inflammation. Cytokine Growth Factor Rev, 2014, 25(3):247-255.
27.	Dianzani I, Howells DW, Ponzone A, et al. Two new mutations in the dihydropteridine reductase gene in patients with tetrahydrobiopterin deficiency. J Med Genet, 1993, 30(6):465-469.
28.	Gu Y, Gong Y, Zhang H, et al. Regulation of transforming growth factor beta 1 gene expression by dihydropteridine reductase in kidney 293T cells. Biochem Cell Biol, 2013, 91(3):187-193.
29.	Wood ZA, Schr?der E, Robin Harris J, et al. Structure, mechanism and regulation of peroxiredoxins. Trends Biochem Sci, 2003, 28(1):32-40.
30.	Kayashima Y, Yamakawa-Kobayashi K. Involvement of Prx3, a Drosophila ortholog of the thiol-dependent peroxidase PRDX3, in agedependent oxidative stress resistance. Biomed Res, 2012, 33(5):319-322.
31.	Pillay CS, Hofmeyr JH, Rohwer JM. The logic of kinetic regulation in the thioredoxin system. BMC Syst Biol, 2011, 5:15.
32.	Smith RW, Cash P, Ellefsen S, et al. Proteomic changes in the crucian carp brain during exposure to anoxia. Proteomics, 2009, 9(8):2217-2229.
33.	Ramana KV, Chandra D, Srivastava S, et al. Aldose reductase mediates mitogenic signaling in vascular smooth muscle cells. J Biol Chem, 2002, 277(35):32063-32070.
34.	Keith RJ, Haberzettl P, Vladykovskaya E, et al. Aldose reductase decreases endop lasmic reticulum stress in ischemic hearts. Chem Biol Interact, 2009, 178(1-3):242-249.
35.	Tammali R, Ramana KV, Singhal SS, et al. Aldose reductase regulates growth factor-induced cyclooxygenase-2 expression and prostaglandin E2 production in human colon cancer cells. Cancer Res, 2006, 66(19): 9705-9713.
36.	Pandey S, Srivastava SK, Ramana KV. A potential therapeutic role for aldose reductase inhibitors in the treatment of endotoxin-related inflammatory diseases. Expert Opin Investig Drugs, 2012, 21(3):329-339.
37.	陳強, 陸江陽, 陸兵勛, 等.環氧合酶-2/CNPase對海洛因海綿狀白質腦病中少突膠質細胞凋亡的作用.中華醫學雜志, 2008, 88(25):857-858.
38.	Gravel M, Robert F, Kottis V, et al. 2', 3'-cycl ic nucleotide 3'-phosphodiesterase: a novel RNA-binding protein that inhibits protein synthesis. J Neurosci Res, 2009, 87(5):1069-1079.

1. Mugabe C, Azghani AO, Omri A. Li posome-mediated gentamicin delivery:development and activity against resistant strains of Pseudomonas aeruginosa isolated from cystic fibrosis patients. J Antimicrob-Chemother, 2005, 55(2):269-271.
2. 王鴻麗.急性脊髓損傷及電針治療大鼠的比較蛋白質組研究.北京:中國人民解放軍軍事醫學科學院, 2009.
3. 黃亞東, 項琪, 張宏波, 等.堿性成纖維細胞生長因子脂質體的制備和評價.中國藥學雜志, 2008, 43(13):992-997.
4. Dos Santos N, Allen C, Doppen AM, et al. Influence of poly(ethylene glycol)grafting density and polymer length on liposomes:relating plasma circulation lifetimes to protein binding. Biochim Biophys Acta, 2007, 1768(6):1367-1377.
5. 王文岳, 楊天府, 劉雷, 等.一種新型脊柱撐開器的研制及脊髓牽張性損傷動物模型的建立.中國修復重建外科雜志, 2011, 25(6):705-710.
6. Yaksh TL, Stevens CW. Simple catheter preparation for permitting bolus intrathecal administration during chronic intrathecal infusion. Pharmacol Biochem Behav, 1986, 25(2):483-485.
7. Fisher M, Meadows ME, Tuyen D, et al. Delayed treatment with intravenous basic fibroblast growth factor reduces infarct size following permanent focal cerebral ischemia in rats. J Cereb FlowMetab, 1995, 15(6):953-959.
8. Ramagli LS. Quantifying protein in 2-D PAGE solubil ization buffers. Methods Mol Biol, 1999, 112:99-103.
9. Crommelin DJA, Daemen T, Scherphof GL, et al. Liposomes:vehicles for the targeted and controlled del ivery of peptides and proteins. J Control Release, 1997, 46(122):165-175.
10. Pan W, Kastin AJ. Penetration of neurotrophins and cytoltines across the blood-brain/blood-spinal cord barrier. Adv Drug Deliv Rev, 1999, 36(2-3):291-298.
11. 王文岳, 楊天府, 劉雷, 等.大鼠急性脊髓牽張損傷相關蛋白質的篩選和鑒定.中華外科雜志, 2012, 50(8):737-743.
12. De Nicola AF, Gonzalez SL, Labombarda F, et al. Progesterone treatment of spinal cord injury. J Mol Neurosci, 2006, 28(1):3-15.
13. 沈正祥, 呂紅斌, 李小明, 等.川芎嗪對大鼠急性脊髓損傷模型caspase-3和NF表達的影響.中南大學學報:醫學版, 2008, 33(8):693-699.
14. 謝玲林, 胡火珍, 楊明理, 等.成年大鼠急性脊髓全橫斷損傷后差異表達蛋白的蛋白組學研究.四川大學學報:自然科學版, 2008, 45(2): 419-425.
15. Dimlich RV, Showers MJ, Shi pley MT. Densitometric analysis of cytochrome oxidase in ischemic rat brain. Brain Res, 1990, 516(2):181-191.
16. 譚小玲, 柳君澤, 曹利飛, 等.長時間缺氧對大鼠腦皮質線粒體細胞色素氧化酶活性及亞基Ⅰ、Ⅳ蛋白表達的影響.中華航空航天醫學雜志, 2002, 13(4):243-246.
17. Nakatsuka H, Ohta S, Tanaka J, et al. Histochemical cytochrome coxidase activity and caspase-3 in gerbil hippocampal CA1 neurons after transient forebrain ischemia. Neurosci Lett, 2000, 285(2):127-130.
18. Soane L, Kahraman S, Kristian T, et al. Mechanisms of impaired mitochondrial energy metabolism in acute and chronic neurodegenerative disorders. J Neurosci Res, 2007, 85(15):3407-3415.
19. Ball RL, Albrecht T, Thompson WC, et al. Thrombin, EGF and phorbol myristate acetate stimulate tubul in polymerization in quiescent cells:A potential link to mitogenesis. Cell Motil Cytoskeleton, 1992, 23(4):265-278.
20. Johnsen JI, Aurelio ON, Kwaja Z, et al. p53-mediated negative regulation of stathmin/Op18 expression is associated with G(2)/M cell-cycle arrest. Int J Cancer, 2000, 88(5):685-691.
21. Manceau V, Gavet O, Curmi P, et al. Stathmin interaction with HSC70 family proteins. Electrophoresis, 1999, 20(2):409-417.
22. Poulsen K, Bahl JM, Simonsen AH. Distinct transthyretin oxidation isoform profile in spinal fluid from patients with Alzheimer's disease and mild cognitive impairment. Clin Proteomics, 2014, 11(1):12.
23. 任惠民, 周志剛, 陳向軍, 等.重癥肌無力患者骨骼肌肉蛋白成分分析.中華神經科雜志, 2000, 33(4):227-230.
24. Cabiscol E, Levine RL. The phosphatase activity of carbonic anhydraseⅢis reversibly regulated by glutathiolation. Proc Natl Acad Sic U S A, 1996, 93(9):4170-4174.
25. Liu FT, Rabinovich GA. Galectins:regulators of acute and chronic inflammation. Ann N Y Acad Sci, 2010, 1183:158-182.
26. Mendez-Huergo SP, Maller SM, Farez MF, et al. Integration of lectinglycan recognition systems and immune cell networks in CNS inflammation. Cytokine Growth Factor Rev, 2014, 25(3):247-255.
27. Dianzani I, Howells DW, Ponzone A, et al. Two new mutations in the dihydropteridine reductase gene in patients with tetrahydrobiopterin deficiency. J Med Genet, 1993, 30(6):465-469.
28. Gu Y, Gong Y, Zhang H, et al. Regulation of transforming growth factor beta 1 gene expression by dihydropteridine reductase in kidney 293T cells. Biochem Cell Biol, 2013, 91(3):187-193.
29. Wood ZA, Schr?der E, Robin Harris J, et al. Structure, mechanism and regulation of peroxiredoxins. Trends Biochem Sci, 2003, 28(1):32-40.
30. Kayashima Y, Yamakawa-Kobayashi K. Involvement of Prx3, a Drosophila ortholog of the thiol-dependent peroxidase PRDX3, in agedependent oxidative stress resistance. Biomed Res, 2012, 33(5):319-322.
31. Pillay CS, Hofmeyr JH, Rohwer JM. The logic of kinetic regulation in the thioredoxin system. BMC Syst Biol, 2011, 5:15.
32. Smith RW, Cash P, Ellefsen S, et al. Proteomic changes in the crucian carp brain during exposure to anoxia. Proteomics, 2009, 9(8):2217-2229.
33. Ramana KV, Chandra D, Srivastava S, et al. Aldose reductase mediates mitogenic signaling in vascular smooth muscle cells. J Biol Chem, 2002, 277(35):32063-32070.
34. Keith RJ, Haberzettl P, Vladykovskaya E, et al. Aldose reductase decreases endop lasmic reticulum stress in ischemic hearts. Chem Biol Interact, 2009, 178(1-3):242-249.
35. Tammali R, Ramana KV, Singhal SS, et al. Aldose reductase regulates growth factor-induced cyclooxygenase-2 expression and prostaglandin E2 production in human colon cancer cells. Cancer Res, 2006, 66(19): 9705-9713.
36. Pandey S, Srivastava SK, Ramana KV. A potential therapeutic role for aldose reductase inhibitors in the treatment of endotoxin-related inflammatory diseases. Expert Opin Investig Drugs, 2012, 21(3):329-339.
37. 陳強, 陸江陽, 陸兵勛, 等.環氧合酶-2/CNPase對海洛因海綿狀白質腦病中少突膠質細胞凋亡的作用.中華醫學雜志, 2008, 88(25):857-858.
38. Gravel M, Robert F, Kottis V, et al. 2', 3'-cycl ic nucleotide 3'-phosphodiesterase: a novel RNA-binding protein that inhibits protein synthesis. J Neurosci Res, 2009, 87(5):1069-1079.

Chinese Journal of Reparative and Reconstructive Surgery

PROTEOMICS STUDY ON EFFECT OF BASIC FIBROBLAST GROWTH FACTOR LONG CIRCULATION LIPOSOME ON SPINAL CORD TRACTION INJURY IN RATS

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content