PRELIMINARY HISTOLOGICAL OBSERVATION OF EFFECT OF EXPANDED CAPSULE ON COSTAL CARTILAGE AUTOGRAFT_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

SHANGQiaoli ,  JIANGHaiyue , ZHOUJiayu , HELeren , YANGQinghua

The 7th Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100144, P. R. China;

Corresponding?author：

JIANGHaiyue, Email: jianghaiyue_psh@163.com

Keywords：

Costal cartilage autograft; Expanded capsule; Histological observation; Rabbit

DOI：

10.7507/1002-1892.20140165

Video：

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Objective To explore the effect of the expanded capsule on the growth of autogenous costal cartilage. Methods Sixteen New Zealand white rabbits at the age of 3 months (weighing, 2.2-2.5 kg; male or female) were selected and four 15 mL tissue expanders were implanted on the back symmetrically. After 1 month, the expanded capsule formed, the tissue expanders were removed; the capsule of the left side was removed (experimental group), and the capsule of the right side was reserved (control group); meanwhile, the right 7th and 8th costal cartilage without the perichondrium was divided into segments and placed into the capsule of 2 groups symmetrically. At 4 and 8 weeks after transplantation, the cartilage was harvested for the general, weighing, and histological observations. Results One rabbit died during the experiment, and the other 15 rabbits survived. The differences of cartilage weight between before and after transplantation showed more obvious increase in the experimental group[(0.003 4±0.002 7) g and (0.005 8±0.001 4) g] than those in the control group[(-0.000 3±0.001 9) g and (-0.003 9±0.005 3) g] at 4 and 8 weeks, showing significant differences between 2 gouprs (t=4.331, P=0.029; t=6.688, P=0.008). The change of cartilage weight at 8 weeks was significantly higher than that at 4 weeks in the experimental group (t=-3.098, P=0.001); but the change of cartilage weight at 8 weeks was significantly lower than that at 4 weeks in the control group (t=2.491, P=0.009). The histological observation showed that the activity of the cartilage was enhanced in 2 groups at 4 and 8 weeks when compared with normal cartilage, and more obvious change was observed in the experimental group than in the control group. And the acellular area was seen in the cartilage at 8 weeks in the control group. The Masson staining results showed that the color was deeper in the experimental group than in the control group. Conclusion The removal of the expanded capsule during operation is beneficial to the growth of autogenous costal cartilage. The results can provide corresponding experimental guidance for the clinical problems.

Citation： SHANGQiaoli, JIANGHaiyue, ZHOUJiayu, HELeren, YANGQinghua. PRELIMINARY HISTOLOGICAL OBSERVATION OF EFFECT OF EXPANDED CAPSULE ON COSTAL CARTILAGE AUTOGRAFT. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(6): 741-744. doi: 10.7507/1002-1892.20140165 Copy

1.	莊洪興, 蔣海越, 潘博, 等. 先天性小耳畸形的皮膚軟組織擴張器法外耳再造術. 中華整形外科雜志, 2006, 22(4):286-289.
2.	Kerem M, Orbay H, Sens?z O. Use of an expander temporoparietal fascial flap tecnique for total auricular reconstruction. Plast Reconstr Surg, 2007, 119(7):2320-2321.
3.	Dashan Y, Haiyue J, Qinghua Y, et al. Technical innovations in ear reconstruction using a skin expander with autogenous cartilage grafts. Plast Reconstr Aesthet Surg, 2008, 61 Suppl 1:S59-69.
4.	Jiang H, Pan B, Lin L, et al. Ten-year experience in microtia reconstruction using expander and autogenous cartilage. Int J Pediatr Otorhinolaryngol, 2008, 72(8):1251-1259.
5.	Brent B. Technical advances in ear reconstruction with autogenous rib cartilage grafts:personal experience with 1200 cases. Plast Reconstr Surg, 1999, 104(2):319-338.
6.	Kajin N, Asatoh H. Reconstruction of the auricle with autogeneous rib cartilage grafts. Adv Otorhinolaryngol, 2014, 75:61-75.
7.	Sivayoham E, Woolford TJ. Current opinion on auricular reconstruction. Curr Opin Otolaryngol Head Neck Surg, 2012, 20(4):287-290.
8.	Qing-Hua Y, Yu-Peng S, Haiyue J, et al. The significance of the biomechanical properties of costal cartilage in the timing of ear reconstruction surgery. Plast Reconstr Aesthet Surg, 2011, 64(6):742-746.
9.	曹春艷, 薛斌. 兔皮膚和肉膜之間放置擴張器的研究. 中華實驗外科雜志, 2013, 30(2):404.
10.	Fran?a DC, de Castro AL, Soubhia AM, et al. Evaluation of the biocompatibility of silicone gel implants-histomorphometric study. Acta Inform Med, 2013, 21(2):93-97.
11.	Pasyk KA, Argenta LC, Austad ED. Histopathology of human expanded tissue. Clin Plast Surg, 1987, 14(3):435:445.
12.	陳惠平, 肖燕, 黃瑩瀅, 等. 早期連續快速擴張法對擴張囊外纖維包膜影響的研究. 中國美容整形外科雜志, 2013, 24(1):28-30.
13.	Tang Y, Luan J, Zhang X. Accelerating tissue expansion by application of topical papaverine cream. Plast Reconstr Surg, 2004, 114(5):1166-1169.
14.	安波, 莊洪興, 孫廣慈, 等. 擴張包膜上植皮成活的臨床觀察. 中華整形外科雜志, 2001, 17(2):112-113.
15.	張志宏, 王佳琦, 李文志. 包膜對豬擴張后動脈筋膜皮瓣血供影響的研究. 中國美容醫學, 2011, 20(2):222-225.
16.	劉學軍, 郝建忠, 喻理. 皮膚軟組織擴張術中維持擴張期對擴張皮膚的影響. 國際醫藥衛生導報, 2002, 8(4):48-49.
17.	劉巖, 馮國平, 楊鍇, 等. 面部擴張手術中纖維包膜的減張作用探討. 中國修復重建外科雜志, 2012, 26(8):939-942.
18.	Walia I, Peer LA, Bernhard WG, et al. Does growth occur in young rabbit ear cartilage grafts transplanted in young rabbits? Plast Reconstr Surg Transplant Bull, 1962, 29(3):259-265.
19.	Gibson T, Davis W. The distortion of autogenous cartilage grafts:Its cause and prevention. Br Plast Surg, 1957-1958, 10:257-274.
20.	李太穎, 周剛, 夏雙印. 自體耳軟骨移植在動脈島狀皮瓣中成活規律的形態學研究. 實用美容整形外科雜志, 2003, 14(4):172-174.
21.	陳琦, 王正敏, 王德輝, 等. 皮膚擴張對自體移植軟骨生長的影響. 上海醫科大學學報, 2000, 27(3):226-228.
22.	Eisemann ML. The growth potential of autograft cartilage. An experimental study. Arch Otolaryngol, 1983, 109(7):469-472.

1. 莊洪興, 蔣海越, 潘博, 等. 先天性小耳畸形的皮膚軟組織擴張器法外耳再造術. 中華整形外科雜志, 2006, 22(4):286-289.
2. Kerem M, Orbay H, Sens?z O. Use of an expander temporoparietal fascial flap tecnique for total auricular reconstruction. Plast Reconstr Surg, 2007, 119(7):2320-2321.
3. Dashan Y, Haiyue J, Qinghua Y, et al. Technical innovations in ear reconstruction using a skin expander with autogenous cartilage grafts. Plast Reconstr Aesthet Surg, 2008, 61 Suppl 1:S59-69.
4. Jiang H, Pan B, Lin L, et al. Ten-year experience in microtia reconstruction using expander and autogenous cartilage. Int J Pediatr Otorhinolaryngol, 2008, 72(8):1251-1259.
5. Brent B. Technical advances in ear reconstruction with autogenous rib cartilage grafts:personal experience with 1200 cases. Plast Reconstr Surg, 1999, 104(2):319-338.
6. Kajin N, Asatoh H. Reconstruction of the auricle with autogeneous rib cartilage grafts. Adv Otorhinolaryngol, 2014, 75:61-75.
7. Sivayoham E, Woolford TJ. Current opinion on auricular reconstruction. Curr Opin Otolaryngol Head Neck Surg, 2012, 20(4):287-290.
8. Qing-Hua Y, Yu-Peng S, Haiyue J, et al. The significance of the biomechanical properties of costal cartilage in the timing of ear reconstruction surgery. Plast Reconstr Aesthet Surg, 2011, 64(6):742-746.
9. 曹春艷, 薛斌. 兔皮膚和肉膜之間放置擴張器的研究. 中華實驗外科雜志, 2013, 30(2):404.
10. Fran?a DC, de Castro AL, Soubhia AM, et al. Evaluation of the biocompatibility of silicone gel implants-histomorphometric study. Acta Inform Med, 2013, 21(2):93-97.
11. Pasyk KA, Argenta LC, Austad ED. Histopathology of human expanded tissue. Clin Plast Surg, 1987, 14(3):435:445.
12. 陳惠平, 肖燕, 黃瑩瀅, 等. 早期連續快速擴張法對擴張囊外纖維包膜影響的研究. 中國美容整形外科雜志, 2013, 24(1):28-30.
13. Tang Y, Luan J, Zhang X. Accelerating tissue expansion by application of topical papaverine cream. Plast Reconstr Surg, 2004, 114(5):1166-1169.
14. 安波, 莊洪興, 孫廣慈, 等. 擴張包膜上植皮成活的臨床觀察. 中華整形外科雜志, 2001, 17(2):112-113.
15. 張志宏, 王佳琦, 李文志. 包膜對豬擴張后動脈筋膜皮瓣血供影響的研究. 中國美容醫學, 2011, 20(2):222-225.
16. 劉學軍, 郝建忠, 喻理. 皮膚軟組織擴張術中維持擴張期對擴張皮膚的影響. 國際醫藥衛生導報, 2002, 8(4):48-49.
17. 劉巖, 馮國平, 楊鍇, 等. 面部擴張手術中纖維包膜的減張作用探討. 中國修復重建外科雜志, 2012, 26(8):939-942.
18. Walia I, Peer LA, Bernhard WG, et al. Does growth occur in young rabbit ear cartilage grafts transplanted in young rabbits? Plast Reconstr Surg Transplant Bull, 1962, 29(3):259-265.
19. Gibson T, Davis W. The distortion of autogenous cartilage grafts:Its cause and prevention. Br Plast Surg, 1957-1958, 10:257-274.
20. 李太穎, 周剛, 夏雙印. 自體耳軟骨移植在動脈島狀皮瓣中成活規律的形態學研究. 實用美容整形外科雜志, 2003, 14(4):172-174.
21. 陳琦, 王正敏, 王德輝, 等. 皮膚擴張對自體移植軟骨生長的影響. 上海醫科大學學報, 2000, 27(3):226-228.
22. Eisemann ML. The growth potential of autograft cartilage. An experimental study. Arch Otolaryngol, 1983, 109(7):469-472.

Chinese Journal of Reparative and Reconstructive Surgery

PRELIMINARY HISTOLOGICAL OBSERVATION OF EFFECT OF EXPANDED CAPSULE ON COSTAL CARTILAGE AUTOGRAFT

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