REPAIR OF LARGE SEGMENTAL BONE DEFECT BY TISSUE ENGINEERED PERIOSTEUM AND DEPROTEINIZED BONE SCAFFOLD IN RABBITS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

TUOZhenhe ¹ ,  ZHAOLin ¹ , WANGShuanke ¹ , LUYongting ² , RENGuangtie ¹ , ZHANGCangyu ¹ , YUJiajia ¹ , SUNRui ¹ , WANGJing ¹

1. Department of Orthopaedics, Orthopaedics Key Laboratory of Gansu Province, the Second Hospital of Lanzhou University, Lanzhou Gansu, 730000, P. R. China;
2. Department of Gynecology, the Second Hospital of Lanzhou University, Lanzhou Gansu, 730000, P. R. China;

Corresponding?author：

ZHAOLin, Email: bonezl@sina.com

Keywords：

Bone tissue engineering; Tissue engineered periosteum; Small intestinal submucosa; Bone marrow mesenchymal stem cells; Deproteinized bone; Rabbit

DOI：

10.7507/1002-1892.20140115

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Objective To evaluate the effect of tissue engineered periosteum on the repair of large diaphysis defect in rabbit radius, and the effect of deproteinized bone (DPB) as supporting scaffolds of tissue engineering periosteum. Methods Bone marrow mesenchymal stem cells (BMSCs) were cultured from 1-month-old New Zealand Rabbit and osteogenetically induced into osteoblasts. Porcine small intestinal submucosa (SIS) scaffold was produced by decellular and a series mechanical and physiochemical procedures. Then tissue engineered periosteum was constructed by combining osteogenic BMSCs and SIS, and then the adhesion of cells to scaffolds was observed by scanning electron microscope (SEM). Fresh allogeneic bone was drilled and deproteinized as DPB scaffold. Tissue engineered periosteum/DPB complex was constructed by tissue engineered periosteum and DPB. Tissue engineered periosteum was "coat-like" package the DPB, and bundled with absorbable sutures. Forty-eight New Zealand white rabbits (4-month-old) were randomly divided into 4 groups (groups A, B, C, and D, n=12). The bone defect model of 3.5 cm in length in the left radius was created. Defect was repaired with tissue engineered periosteum in group A, with DPB in group B, with tissue engineered periosteum/DPB in group C; defect was untreated in group D. At 4, 8, and 12 weeks after operation, 4 rabbits in each group were observed by X-ray. At 8 weeks after operation, 4 rabbits of each group were randomly sacrificed for histological examination. Results SEM observation showed that abundant seeding cells adhered to tissue engineered periosteum. At 4, 8, and 12 weeks after operation, X-ray films showed the newly formed bone was much more in groups A and C than groups B and D. The X-ray film score were significantly higher in groups A and C than in groups B and D, in group A than in group C, and in group B than in group D (P<0.05). Histological staining indicated that there was a lot of newly formed bone in the defect space in group A, with abundant newly formed vessels and medullary cavity. While in group B, the defect space filled with the DPB, the degradation of DPB was not obvious. In group C, there was a lot of newly formed bone in the defect space, island-like DPB and obvious DPB degradation were seen in newly formed bone. In group D, the defect space only replaced by some connective tissue. Conclusion Tissue engineered periosteum constructed by SIS and BMSCs has the feasibility to repair the large diaphysis defect in rabbit. DPB isn't an ideal support scaffold of tissue engineering periosteum, the supporting scaffolds of tissue engineered periosteum need further exploration.

Citation： TUOZhenhe, ZHAOLin, WANGShuanke, LUYongting, RENGuangtie, ZHANGCangyu, YUJiajia, SUNRui, WANGJing. REPAIR OF LARGE SEGMENTAL BONE DEFECT BY TISSUE ENGINEERED PERIOSTEUM AND DEPROTEINIZED BONE SCAFFOLD IN RABBITS. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(4): 511-516. doi: 10.7507/1002-1892.20140115 Copy

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6.	貝抗勝, 吳禮楊, 孫慶文, 等. 人骨膜細胞生物學特性的實驗研究. 中華創傷骨科雜志, 2011, 13(12):1170-1174.
7.	李衛國, 黃向紅, 唐景清, 等. 骨膜成骨細胞培養活體自體、異體和異種移植成骨的實驗研究. 昆明醫學院學報, 2008, 29(1):81-84.
8.	Soldado F, Fontecha CG, Barber I, et al. Vascularized fibular periosteal graft:a new technique to enhance bone union in children. J Pediatr Orthop, 2012, 32(3):308-313.
9.	吳偉熾, 黃東, 劉曉春, 等. 穿支骨膜瓣游離移植治療難治性骨不連的療效觀察. 現代醫院, 2013, 13(3):39-41.
10.	趙琳, 史志勇, 周晟, 等. 組織工程骨膜異體體內成骨修復兔骨缺損的初步觀察. 中國修復重建外科雜志, 2008, 22(2):145-147.
11.	Muschler GF, Nakamoto C, Griffith LG. Engineering principles of clinical cell-based tissue engineering. J Bone Joint Surg (Am), 2004, 86-A(7):1541-1558.
12.	王金成, 李志洲, 陳偉, 等. 骨庫骨的生物力學特性比較研究. 骨與關節損傷雜志, 2001, 16(1):35-36.
13.	簡月奎, 羅陽, 田曉濱, 等. 改良法制備異種脫蛋白骨體內植入修復骨缺損的免疫學分析. 中國組織工程研究與臨床康復, 2010, 14(42):7802-7806.

1. 許建中. 骨組織工程相關研究新進展. 中國修復重建外科雜志, 2010, 24(7):769-773.
2. Zhao L, Zhao JL, Wan L, et al. The study of the feasibility of segmental bone defect repair with tissue-engineered bone membrane:a qualitative observation. Strategies Trauma Limb Reconstr, 2008, 3(2):57-64.
3. 楊志明. 組織工程. 北京:化學工業出版社, 2002:225-227.
4. 范偉, 安洪, 蔣電明, 等. 脫蛋白骨為支架材料體外構建的組織工程骨. 中華醫學雜志, 2006, 86(47):3349-3352.
5. Lane JM, Sandhu HS. Current approaches to experimental bone grafting. Orthop Clin North Am, 1987, 18(2):213-225.
6. 貝抗勝, 吳禮楊, 孫慶文, 等. 人骨膜細胞生物學特性的實驗研究. 中華創傷骨科雜志, 2011, 13(12):1170-1174.
7. 李衛國, 黃向紅, 唐景清, 等. 骨膜成骨細胞培養活體自體、異體和異種移植成骨的實驗研究. 昆明醫學院學報, 2008, 29(1):81-84.
8. Soldado F, Fontecha CG, Barber I, et al. Vascularized fibular periosteal graft:a new technique to enhance bone union in children. J Pediatr Orthop, 2012, 32(3):308-313.
9. 吳偉熾, 黃東, 劉曉春, 等. 穿支骨膜瓣游離移植治療難治性骨不連的療效觀察. 現代醫院, 2013, 13(3):39-41.
10. 趙琳, 史志勇, 周晟, 等. 組織工程骨膜異體體內成骨修復兔骨缺損的初步觀察. 中國修復重建外科雜志, 2008, 22(2):145-147.
11. Muschler GF, Nakamoto C, Griffith LG. Engineering principles of clinical cell-based tissue engineering. J Bone Joint Surg (Am), 2004, 86-A(7):1541-1558.
12. 王金成, 李志洲, 陳偉, 等. 骨庫骨的生物力學特性比較研究. 骨與關節損傷雜志, 2001, 16(1):35-36.
13. 簡月奎, 羅陽, 田曉濱, 等. 改良法制備異種脫蛋白骨體內植入修復骨缺損的免疫學分析. 中國組織工程研究與臨床康復, 2010, 14(42):7802-7806.

Chinese Journal of Reparative and Reconstructive Surgery

REPAIR OF LARGE SEGMENTAL BONE DEFECT BY TISSUE ENGINEERED PERIOSTEUM AND DEPROTEINIZED BONE SCAFFOLD IN RABBITS

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