PRIMARY STUDY ON TISSUE ENGINEERED PERIOSTEUM OSTEOGENESIS TO REPAIR SCAPULA DEFECT IN VIVO IN ALLOGENIC RABBIT_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

ZHANGCangyu , WANGShuanke , RENGuangtie , TUOZhenhe , YUJiajia , WANGJing , ANLiping , MAJinglin ,  ZHAOLin

Department of Orthopedics, the 2nd Hospital of Lanzhou University, the Bone and Joint Key Laboratory of Gansu Province, Lanzhou Gansu, 730030, P. R. China;

Corresponding?author：

ZHAOLin, Email: bonezl@sina.com

Keywords：

Tissue engineered periosteum; Bone marrow mesenchymal stem cells; Small intestinal submucosa; Bone defect repair; Rabbit; Pig

DOI：

10.7507/1002-1892.20140085

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the feasibility of tissue engineered periosteum (TEP) constructed by porcine small intestinal submucosa (SIS) and bone marrow mesenchymal stem cells (BMSCs) of rabbit to repair the large irregular bone defects in allogenic rabbits. Methods The BMSCs were cultivated from the bone marrow of New Zealand white rabbits (aged, 2 weeks-1 month). SIS was fabricated by porcine proximal jejunum. The TEP constructed by SIS scaffold and BMSCs was prepared in vitro. Eighteen 6-month-old New Zealand white rabbits whose scapula was incompletely resected to establish one side large irregular bone defects (3 cm×3 cm) model. The bone defects were repaired with TEP (experimental group,n=9) and SIS (control group,n=9), respectively. At 8 weeks after operation, the rabbits were sacrificed, and the implants were harvested. The general condition of the rabbits was observed; X-ray radiography and score according to Lane-Sandhu criteria, and histological examination (HE staining and Masson staining) were performed. Results After operation, all animals had normal behavior and diet; the incision healed normally. The X-ray results showed new bone formation with normal bone density in the defect area of experimental group; but no bone formation was observed in control group. The X-ray score was 6.67±0.32 in experimental group and was 0.32±0.04 in control group, showing significant difference (t=19.871,P=0.001). The general observation of the specimens showed bone healing at both ends of the defect, and the defect was filled by new bone in experimental group; no new bone formed in the control group. The histological staining showed new bone tissue where there were a lot of new vessels and medullary cavity, and no macrophages or lymphocytes infiltration was observed in the defect area of experimental group; only some connective tissue was found in the control group. Conclusion TEP constructed by porcine SIS and BMSCs of rabbit can form new bone in allogenic rabbit and has the feasibility to repair the large irregular bone defects.

Citation： ZHANGCangyu, WANGShuanke, RENGuangtie, TUOZhenhe, YUJiajia, WANGJing, ANLiping, MAJinglin, ZHAOLin. PRIMARY STUDY ON TISSUE ENGINEERED PERIOSTEUM OSTEOGENESIS TO REPAIR SCAPULA DEFECT IN VIVO IN ALLOGENIC RABBIT. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(3): 384-388. doi: 10.7507/1002-1892.20140085 Copy

1.	Ikada Y. Challenges in tissue engineering. J R Soc Interface, 2006, 3(10):589-601.
2.	Augustin G, Antabak A, Davila S. The periosteum. Part 1:Anatomy, histology and molecular biology. Injury, 2007, 38(10):1115-1130.
3.	Dwek JR. The periosteum:what is it, where is it, and what mimics it in its absence? Skeletal Radiol, 2010, 39(4):319-323.
4.	Mackie EJ, Ahmed YA, Tatarczuch L, et al. Endochondral ossification:how cartilage is converted into bone in the developing skeleton. Int J Biochem Cell Biol, 2008, 40(1):46-62.
5.	Hall BK, Miyake T. All for one and one for all:condensations and the initiation of skeletal development. Bioessays, 2000, 22(2):138-147.
6.	張大偉, 田清業, 劉光軍, 等. 骨膜瓣復合異體骨移植修復大段骨缺損. 組織工程與重建外科, 2012, 8(1):26-31.
7.	Mizuno H, Hata KI, Kojima K, et al. A novel approach to regenerating periodontal tissue by grafting autologous cultured periosteum. Tissue Eng, 2006, 12(5):1227-1335.
8.	Zhao L, Zhao J, Wang S, et al. Comparative study between tissue-engineered periosteum and structural allograft in rabbit critical-sized radial defect model. J Biomed Mater Res B Appl Biomater, 2011, 97(1):1-9.
9.	庾佳佳, 汪新柱, 趙琳, 等. 兔骨髓間充質干細胞的分離培養及成骨誘導. 中國組織工程研究, 2013, 17(6):974-979.
10.	Gersbach CA, Le Doux JM, Guldberg RE, et al. Inducible regulation of Runx2-stimulated osteogenesis. Gene Ther, 2006, 13(11):873-882.
11.	Tang YL, Zhao Q, Zhang YC, et al. Autologous mesenchymal stem cell transplantation induce VEGF and neovascularization in ischemic myocardium. Regul Pept, 2004, 117(1):3-10.
12.	Abraham GA, Murray J, Billiar K, et al. Evaluation of the porcine intestinal collagen layer as a biomaterial. J Biomed Mater Res, 2000, 51(3):442-452.
13.	Xiao ZM, Jiang H, Zhan XL, et al. Treatment of osteonecrosis of femoral head with BMSCs-seeded bio-derived bone materials combined with rhBMP-2 in rabbits. Chin J Traumatol, 2008, 11(3):165-170.
14.	趙琳, 史志勇, 周晟, 等. 組織工程骨膜異體體內成骨修復兔骨缺損的初步觀察. 中國修復重建外科雜志, 2008, 22(2):145-147.
15.	Zhao L, Zhao JL, Wan L, et al. The study of the feasibility of segmental bone defect repair with tissue-engineered bone membrane:a qualitative observation. Strategies Trauma Limb Reconstr, 2008, 3(2):57-64.
16.	李偉. 肩胛骨的臨床解剖學研究. 廣州:南方醫科大學, 2007.
17.	Meirelles Lda S, Fontes AM, Covas DT, et al. Mechanisms involved in the therapeutic properties of mesenchymal stem cells. Cytokine Growth Factor Rev, 2009, 20(5):419-427.
18.	Hodde J, Janis A, Hiles M. Effects of sterilization on an extracellular matrix scaffold:part II. Bioactivity and matrix interaction. J Mater Sci Mater Med, 2007, 18(4):545-550.
19.	Ye H, Xia Z, Ferguson DJ, et al. Studies on the use of hollow fibre membrane bioreactors for tissue generation by using rat bone marrow fibroblastic cells and a composite scaffold. J Mater Sci Mater Med, 2007, 18(4):641-648.
20.	裴國獻, 魏寬海. 顯微外科與血管化組織工程組織的構建. 中華創傷骨科雜志, 2004, 6(4):361-363.
21.	Ye H, Das DB, Triffitt JT, et al. Modelling nutrient transport in hollow fibre membrane bioreactors for growing three-dimensional bone tissue. J Membrane Sci, 2006, 272(1-2):169-178.
22.	羅靜聰, 楊志明. 小腸黏膜下層的制備及其特性的研究進展. 中國修復重建外科雜志, 2003, 17(5):425-428.

1. Ikada Y. Challenges in tissue engineering. J R Soc Interface, 2006, 3(10):589-601.
2. Augustin G, Antabak A, Davila S. The periosteum. Part 1:Anatomy, histology and molecular biology. Injury, 2007, 38(10):1115-1130.
3. Dwek JR. The periosteum:what is it, where is it, and what mimics it in its absence? Skeletal Radiol, 2010, 39(4):319-323.
4. Mackie EJ, Ahmed YA, Tatarczuch L, et al. Endochondral ossification:how cartilage is converted into bone in the developing skeleton. Int J Biochem Cell Biol, 2008, 40(1):46-62.
5. Hall BK, Miyake T. All for one and one for all:condensations and the initiation of skeletal development. Bioessays, 2000, 22(2):138-147.
6. 張大偉, 田清業, 劉光軍, 等. 骨膜瓣復合異體骨移植修復大段骨缺損. 組織工程與重建外科, 2012, 8(1):26-31.
7. Mizuno H, Hata KI, Kojima K, et al. A novel approach to regenerating periodontal tissue by grafting autologous cultured periosteum. Tissue Eng, 2006, 12(5):1227-1335.
8. Zhao L, Zhao J, Wang S, et al. Comparative study between tissue-engineered periosteum and structural allograft in rabbit critical-sized radial defect model. J Biomed Mater Res B Appl Biomater, 2011, 97(1):1-9.
9. 庾佳佳, 汪新柱, 趙琳, 等. 兔骨髓間充質干細胞的分離培養及成骨誘導. 中國組織工程研究, 2013, 17(6):974-979.
10. Gersbach CA, Le Doux JM, Guldberg RE, et al. Inducible regulation of Runx2-stimulated osteogenesis. Gene Ther, 2006, 13(11):873-882.
11. Tang YL, Zhao Q, Zhang YC, et al. Autologous mesenchymal stem cell transplantation induce VEGF and neovascularization in ischemic myocardium. Regul Pept, 2004, 117(1):3-10.
12. Abraham GA, Murray J, Billiar K, et al. Evaluation of the porcine intestinal collagen layer as a biomaterial. J Biomed Mater Res, 2000, 51(3):442-452.
13. Xiao ZM, Jiang H, Zhan XL, et al. Treatment of osteonecrosis of femoral head with BMSCs-seeded bio-derived bone materials combined with rhBMP-2 in rabbits. Chin J Traumatol, 2008, 11(3):165-170.
14. 趙琳, 史志勇, 周晟, 等. 組織工程骨膜異體體內成骨修復兔骨缺損的初步觀察. 中國修復重建外科雜志, 2008, 22(2):145-147.
15. Zhao L, Zhao JL, Wan L, et al. The study of the feasibility of segmental bone defect repair with tissue-engineered bone membrane:a qualitative observation. Strategies Trauma Limb Reconstr, 2008, 3(2):57-64.
16. 李偉. 肩胛骨的臨床解剖學研究. 廣州:南方醫科大學, 2007.
17. Meirelles Lda S, Fontes AM, Covas DT, et al. Mechanisms involved in the therapeutic properties of mesenchymal stem cells. Cytokine Growth Factor Rev, 2009, 20(5):419-427.
18. Hodde J, Janis A, Hiles M. Effects of sterilization on an extracellular matrix scaffold:part II. Bioactivity and matrix interaction. J Mater Sci Mater Med, 2007, 18(4):545-550.
19. Ye H, Xia Z, Ferguson DJ, et al. Studies on the use of hollow fibre membrane bioreactors for tissue generation by using rat bone marrow fibroblastic cells and a composite scaffold. J Mater Sci Mater Med, 2007, 18(4):641-648.
20. 裴國獻, 魏寬海. 顯微外科與血管化組織工程組織的構建. 中華創傷骨科雜志, 2004, 6(4):361-363.
21. Ye H, Das DB, Triffitt JT, et al. Modelling nutrient transport in hollow fibre membrane bioreactors for growing three-dimensional bone tissue. J Membrane Sci, 2006, 272(1-2):169-178.
22. 羅靜聰, 楊志明. 小腸黏膜下層的制備及其特性的研究進展. 中國修復重建外科雜志, 2003, 17(5):425-428.

Previous Article
PREPARATION AND CHARACTERIZATION OF ACELLULAR ADIPOSE TISSUE MATRIX
Next Article
吻合掌側靜脈且保留指甲的多指指尖離斷再植

Chinese Journal of Reparative and Reconstructive Surgery

PRIMARY STUDY ON TISSUE ENGINEERED PERIOSTEUM OSTEOGENESIS TO REPAIR SCAPULA DEFECT IN VIVO IN ALLOGENIC RABBIT

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content