Expression and diagnostic value of long non-coding RNA lnc-PAPSS2-2 in peripheral blood of active pulmonary tuberculosis patients_West China Medical Journal

Authors：

SONG Jiajia , BAI Mengge , LIU Tangyuheng , HU Xuejiao , ZHAO Zhenzhen , PENG Wu , BAI Hao , WU Qian ,  YING Binwu

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding?author：

YING Binwu, Email: binwuying@126.com

Keywords：

Pulmonary tuberculosis; Long non-coding RNA; Lnc-PAPSS2-2; Diagnosis

DOI：

10.7507/1002-0179.201807068

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ObjectiveTo evaluate the expression level and diagnostic value of lnc-PAPSS2-2 (lnc-PA) in peripheral blood of active pulmonary tuberculosis (PTB) patients.MethodsFrom January 2011 to January 2018, 798 patients with active PTB and 1 650 healthy people undergoing health examination in West China Hospital of Sichuan University and their electronic health records (EHR) were collected. Peripheral blood lnc-PA levels were quantified by quantitative real-time polymerase chain reaction method. The data of lnc-PA and EHR were modeled using nomogram, and the receiver operating characteristic (ROC) curves of lnc-PA, EHR and the combination of lnc-PA and EHR were compared to evaluate the diagnostic value of lnc-PA for active PTB.ResultsThe level of lnc-PA was lower in active PTB patients than that in healthy controls (P<0.001). The areas under ROC curve of lnc-PA, EHR and their combination were 0.619, 0.962, and 0.964 in the training set and 0.626, 0.950, and 0.950 in the validation set, respectively.ConclusionThe diagnostic ability of lnc-PA is poor and that of EHR is good, which indicates that the clinical value of lnc-PA as a biomarker of active PTB remains to be further explored.

Citation： SONG Jiajia, BAI Mengge, LIU Tangyuheng, HU Xuejiao, ZHAO Zhenzhen, PENG Wu, BAI Hao, WU Qian, YING Binwu. Expression and diagnostic value of long non-coding RNA lnc-PAPSS2-2 in peripheral blood of active pulmonary tuberculosis patients. West China Medical Journal, 2018, 33(8): 953-957. doi: 10.7507/1002-0179.201807068 Copy

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3. 李文文, 馮喜英, 關巍. 結核病診斷與治療的研究進展. 中華肺部疾病雜志: 電子版, 2016, 9(2): 204-206.
4. Huarte M. The emerging role of lncRNAs in cancer. Nat Med, 2015, 21(11): 1253-1261.
5. Yang X, Yang J, Wang J, et al. Microarray analysis of long noncoding RNA and mRNA expression profiles in human macrophages infected with Mycobacterium tuberculosis. Sci Rep, 2016, 6: 38963.
6. Sabir N, Hussain T, Shah SZA, et al. miRNAs in tuberculosis: new avenues for diagnosis and host-directed therapy. Front Microbiol, 2018, 9: 602.
7. Carpenter S, Fitzgerald KA. Transcription of inflammatory genes: long noncoding RNA and beyond. J Interferon Cytokine Res, 2015, 35(2): 79-88.
8. Li Z, Rana TM. Decoding the noncoding: prospective of lncRNA-mediated innate immune regulation. RNA Biol, 2014, 11(8): 979-985.
9. Fu Y, Xu X, Xue J, et al. Deregulated lncRNAs in B cells from patients with active tuberculosis. PLoS One, 2017, 12(1): e0170712.
10. He J, Ou Q, Liu C, et al. Differential expression of long non-coding RNAs in patients with tuberculosis infection. Tuberculosis (Edinb), 2017, 107: 73-79.
11. Doolan DL, Apte SH, Proietti C. Genome-based vaccine design: the promise for malaria and other infectious diseases. Int J Parasitol, 2014, 44(12): 901-913.
12. Huang G, Redelman-Sidi G, Rosen N, et al. Inhibition of mycobacterial infection by the tumor suppressor PTEN. J Biol Chem, 2012, 287(27): 23196-23202.
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14. Thao LTP, Heemskerk AD, Geskus RB, et al. Prognostic models for 9-month mortality in tuberculous meningitis. Clin Infect Dis, 2018, 66(4): 523-532.
15. Pontual Y, Pacheco VSS, Monteiro SP, et al. ABCB1 gene polymorphism associated with clinical factors can predict drug-resistant tuberculosis. Clin Sci (Lond), 2017, 131(15): 1831-1840.

West China Medical Journal

Expression and diagnostic value of long non-coding RNA lnc-PAPSS2-2 in peripheral blood of active pulmonary tuberculosis patients

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