Expression of CD34 and polyclone carcinoembryonic antigen detected by puncture biopsy of positive and negative alpha fetoprotein in human hepatocellular carcinoma tissue and its significance of pathological diagnosis_West China Medical Journal

Authors：

LI Wei , WANG Hongli , LI Hong , CHEN Su , YU Meiying , ZHOU Qiuyuan , XU Yanhua ,  QU Yangang

Department of Pathology, the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei 445000, P. R. China;

Corresponding?author：

QU Yangang, Email: 1324322271@qq.com

Keywords：

Hepatocellular carcinoma; Alpha fetoprotein; CD34; Polyclone carcinoembryonic antigen; Immunohistochemistry; Pathological diagnosis

DOI：

10.7507/1002-0179.201606041

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Objective To explore the expression of CD34 and polyclone carcinoembryonic antigen (pCEA) of positive and negative alpha fetoprotein (AFP) detected by puncture biopsy in human hepatocellular carcinoma (HCC) and the significance of pathological diagnosis. Methods Fifty-four HCC tissue specimens from 2013 to 2015 were collected from tumor biopsy samples which confirmed by pathology in the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture. The tissue samples were divided into positive AFP group (n=33) and negative AFP group (n=21) according to the detection results of serology and immunohistochemistry analysis of AFP. Expressions of CD34 and pCEA in the fifty-four HCC specimens were detected by immunohistochemistry. Results The positvie expression rate of pCEA in the positive AFP group was 69.7%, which was significantly higher than that in the negative AFP group (38.1%) (P<0. 05). However, the difference in positive expression rate of CD34 between the positive and negative AFP groups (90.91% and 85.71%, respectively) was not significant (P>0.05). Conclusion The associated detection of AFP, pCEA and CD34 in HCC tissues might contribute to the pathological and differential diagnosis of human hepatocellular carcinoma in puncture biopsies.

Citation： LI Wei, WANG Hongli, LI Hong, CHEN Su, YU Meiying, ZHOU Qiuyuan, XU Yanhua, QU Yangang. Expression of CD34 and polyclone carcinoembryonic antigen detected by puncture biopsy of positive and negative alpha fetoprotein in human hepatocellular carcinoma tissue and its significance of pathological diagnosis. West China Medical Journal, 2017, 32(8): 1195-1198. doi: 10.7507/1002-0179.201606041 Copy

1.	El-serag HB, Rudolph KL. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology, 1994, 132(7): 25571-2576.
2.	林黎娟, 金政, 王研, 等. 超聲引導下肝癌穿刺組織中 DEK 表達的臨床意義. 遼東學院學報: 自然科學版, 2011, 18(1): 50-52, 58.
3.	Ahmad M, Riley TR. Can one predict when ultrasound will be useful with percutaneous liver biopsy? Am J Gastroenterol, 2001, 96(2): 547-549.
4.	歐陽茂, 李錫壽, 李會斌, 等. 肝穿刺活檢經驗總結——附 949 例分析. 上海醫學, 1999, 22(3): 189.
5.	叢文銘, 譚璐, 張樹輝, 等. 肝癌免疫組化診斷譜的研究和應用. 中華腫瘤雜志, 2002, 24(6): 553-556.
6.	閆偉偉, 黃昂, 李永綱, 等. CD34 和 CD117 在人肝癌組織中的表達及其臨床意義. 中華肝臟病雜志, 2011, 19(8): 588-593.
7.	左婷婷, 鄭榮壽, 曾紅梅, 等. 中國肝癌發病狀況與趨勢分析. 中華腫瘤雜志, 2015, 37(9): 691-696.
8.	Srivatanakul P, Sriplung H, Deerasamee S. Epidemiology of liver cancer: an overview. Asian Pac J Cancer Prev, 2004, 5(2): 118-125.
9.	But DY, Lai CL, Yuen MF. Natural history of hepatitis-related hepatocellular carcinoma. World J Gastroenterol, 2008, 14(11): 1652-1656.
10.	陳建國, 陸建華, 朱源榮, 等. 乙型肝炎病毒感染與肝癌發生的 31 年隨訪研究. 中華流行病學雜志, 2010, 31(7): 721-726.
11.	Lau SK, Prakash S, Geller SA, et al. Comparative immunohistochemical profile of hepatocellular carcinoma, cholangiocarcinoma, and metastatic adenocarcinoma. Hum Pathol, 2002, 33(12): 1175-1181.
12.	范公忍, 汪毅, 胡大榮. TSGF、AFP、SF 聯合檢測對原發性肝癌診斷意義的評價. 上海免疫學雜志, 2000, 20(5): 317-317.
13.	叢文銘, 朱世能. 肝膽腫瘤診斷外科病理學. 上海: 上海科技教育出版社, 2002: 132-164.
14.	Soresi M, Magliarisi C, Campagna P, et al. Usefulness of alpha-fetoprotein in the diagnosis of hepatocellular carcinoma. Anticancer Res, 2003, 23(2C): 1747-1753.
15.	Zimmerman RL, Logani S, Baloch Z. Evaluation of the CD34 and CD10 immunostains using a two-color staining protocol in liver fine-needle aspiration biopsies. Diagn Cytopathol, 2005, 32(2): 88-91.
16.	Murakata LA, Ishak KG, Nzeako UC. Clear cell carcinoma of the liver: a comparative immunohistochemical study with renal clear cell carcinoma. Mod Pathol, 2000, 13(8): 874-881.
17.	Ohmori S, Shiraki K, Sugimoto K, et al. High expression of CD34-positive sinusoidal endothelial cells is a risk factor for hepatocellular carcinoma in patients with HCV-associated chronic liver diseases. Hum Pathol, 2001, 32(12): 1363-1370.

1. El-serag HB, Rudolph KL. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology, 1994, 132(7): 25571-2576.
2. 林黎娟, 金政, 王研, 等. 超聲引導下肝癌穿刺組織中 DEK 表達的臨床意義. 遼東學院學報: 自然科學版, 2011, 18(1): 50-52, 58.
3. Ahmad M, Riley TR. Can one predict when ultrasound will be useful with percutaneous liver biopsy? Am J Gastroenterol, 2001, 96(2): 547-549.
4. 歐陽茂, 李錫壽, 李會斌, 等. 肝穿刺活檢經驗總結——附 949 例分析. 上海醫學, 1999, 22(3): 189.
5. 叢文銘, 譚璐, 張樹輝, 等. 肝癌免疫組化診斷譜的研究和應用. 中華腫瘤雜志, 2002, 24(6): 553-556.
6. 閆偉偉, 黃昂, 李永綱, 等. CD34 和 CD117 在人肝癌組織中的表達及其臨床意義. 中華肝臟病雜志, 2011, 19(8): 588-593.
7. 左婷婷, 鄭榮壽, 曾紅梅, 等. 中國肝癌發病狀況與趨勢分析. 中華腫瘤雜志, 2015, 37(9): 691-696.
8. Srivatanakul P, Sriplung H, Deerasamee S. Epidemiology of liver cancer: an overview. Asian Pac J Cancer Prev, 2004, 5(2): 118-125.
9. But DY, Lai CL, Yuen MF. Natural history of hepatitis-related hepatocellular carcinoma. World J Gastroenterol, 2008, 14(11): 1652-1656.
10. 陳建國, 陸建華, 朱源榮, 等. 乙型肝炎病毒感染與肝癌發生的 31 年隨訪研究. 中華流行病學雜志, 2010, 31(7): 721-726.
11. Lau SK, Prakash S, Geller SA, et al. Comparative immunohistochemical profile of hepatocellular carcinoma, cholangiocarcinoma, and metastatic adenocarcinoma. Hum Pathol, 2002, 33(12): 1175-1181.
12. 范公忍, 汪毅, 胡大榮. TSGF、AFP、SF 聯合檢測對原發性肝癌診斷意義的評價. 上海免疫學雜志, 2000, 20(5): 317-317.
13. 叢文銘, 朱世能. 肝膽腫瘤診斷外科病理學. 上海: 上海科技教育出版社, 2002: 132-164.
14. Soresi M, Magliarisi C, Campagna P, et al. Usefulness of alpha-fetoprotein in the diagnosis of hepatocellular carcinoma. Anticancer Res, 2003, 23(2C): 1747-1753.
15. Zimmerman RL, Logani S, Baloch Z. Evaluation of the CD34 and CD10 immunostains using a two-color staining protocol in liver fine-needle aspiration biopsies. Diagn Cytopathol, 2005, 32(2): 88-91.
16. Murakata LA, Ishak KG, Nzeako UC. Clear cell carcinoma of the liver: a comparative immunohistochemical study with renal clear cell carcinoma. Mod Pathol, 2000, 13(8): 874-881.
17. Ohmori S, Shiraki K, Sugimoto K, et al. High expression of CD34-positive sinusoidal endothelial cells is a risk factor for hepatocellular carcinoma in patients with HCV-associated chronic liver diseases. Hum Pathol, 2001, 32(12): 1363-1370.

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Expression of CD34 and polyclone carcinoembryonic antigen detected by puncture biopsy of positive and negative alpha fetoprotein in human hepatocellular carcinoma tissue and its significance of pathological diagnosis

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