Effects of Pilose Antler Blood Wine on Bone Mineral Density and T-lymphocyte Subsets in Ovariectomized Female Rats_West China Medical Journal

Authors：

 JiangHua ^1,2 , HeChengqi ^2,3 , OuYuping ² , YuanJulian ² , YangYang ² , SunZengchun ² , JiaXue ² ,  ZhouXue ¹

1. Department of Histology, Embryology and Neurobiology, West China School of Preclinical and Forensic Medicine;
2. Center of Rehabilitation Medicine, West China Hospital;Sichuan University, Chengdu, Sichuan 610041, P. R. China;
3. Institute of Disaster Management and Reconstruction, Sichuan University-The Hong Kong Polytechnic University, Chengdu, Sichuan 610041, P. R. China;

Corresponding?author：

ZhouXue, Email: zhouxue7239@163.com

Keywords：

Pilose antler blood wine; Osteoporosis; Bone mineral density; Immune function

DOI：

10.7507/1002-0179.20160076

Video：

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Objective To explore the effects of pilose antler blood wine on bone mineral density and T-lymphocyte subsets of ovariectomized female Sprague Dawley (SD) rats. Methods Sixty female SD rats were randomly divided into six groups:normal control group, osteoporosis model group, pure ethylalcohol group, high dose of pilose antler blood wine group, medium dose of pilose antler blood wine group and low dose of pilose antler blood wine group. Normal control group and osteoporosis model group were given saline with the dose of 9.1 g/(kg·d). Pure ethylalcohol group was given pure ethylalcohol with the dose of 9.1 g/(kg·d). The doses of pilose antler blood wine were 4.5, 9.1 and 13.6 g/(kg·d) respectively in low, medium and high dose of pilose antler blood wine group. After the feeding of pilose antler blood wine for 30 days, the bone mineral density and T-lymphocyte subsets of rats were assessed. Results The difference in bone mineral density among the three pilose antler blood wine groups was significant (P<0.05); the most obvious improvement of one mineral density was found in low and medium dose of pilose antler blood wine groups (P<0.05). The CD4⁺ T cells and CD4⁺/CD8⁺ significantly increased and CD8⁺ T cells proportion decreased significantly in pilose antler blood wine group compared with those in the osteoporosis model group (P<0.05). CD4⁺ T cells in medium dose group increased obviously compared with that in the high dose group (P<0.05). CD4⁺/CD8⁺ in low and medium dose groups obviously increased compared with that in high dose group (P<0.05). CD4⁺ T cells of medium dose group obviously increased compared with that in pure ethylalcohol group (P<0.05). CD8⁺ T cells in medium and low dose groups obviously decreased compared that in pure ethylalcohol group (P<0.05). CD4⁺/CD8⁺ in the three dose groups increased significantly compared with that in pure ethylalcohol group (P<0.05). Conclusion Tree different doses of pilose antler blood wine could prevent the osteoporosis and improve the immune function. The effects of medium and low dose of groups were more obvious.

Citation： JiangHua, HeChengqi, OuYuping, YuanJulian, YangYang, SunZengchun, JiaXue, ZhouXue. Effects of Pilose Antler Blood Wine on Bone Mineral Density and T-lymphocyte Subsets in Ovariectomized Female Rats. West China Medical Journal, 2016, 31(2): 283-286. doi: 10.7507/1002-0179.20160076 Copy

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1. 何成奇. 骨質疏松癥的康復治療技術[M]. 北京:人民衛生出版社, 2008:1-19.
2. 楊永紅, 何成奇, 楊霖, 等. 不同強度脈沖電磁場對去勢大鼠血清雌二醇的影響[J]. 四川大學學報:醫學版, 2008, 39(2):256-258.
3. 張月峰, 韓景獻. 骨質疏松動物模型研究進展[J]. 動物醫學進展, 2005, 26(3):8-11.
4. 楊建虹, 費聿榮, 曹毅, 等. 鹿茸血處理前后去勢大鼠股骨的SRXRF分析[J]. 核技術, 2007, 30(5):407-410.
5. 高強, 楊揚, 孫增春, 等. 鹿茸血酒對勢大鼠的升雌激素與抗氧化作用的初步研究[J].四川大學學報:醫學版, 2014, 45(1):134-137.
6. 黃鑫, 孟國林, 劉建. 免疫因素與骨質疏松[J]. 中國骨質疏松雜志, 2010, 16(3):225-228.
7. 郭錦麗, 耿俊梅, 蘇芙蓉, 等. 酒精性骨疾病發生機制的研究現狀[J]. 實用骨科雜志, 2009, 15(2):111-114.
8. Holbrook T, Barrett-Connor E. A prospective study of alcohol consumption and bone mineral density[J]. BMJ, 1993, 306(6891):1506-1509.
9. Kim MJ, Shim MS, Kim MK, et al. Effect of chronic alcohol ingestion on bone mineral density in males without liver cirrhosis[J]. Korean J Intern Med, 2003, 18(3):174-180.
10. Reagan-Shaw S, Nihal M, Ahmad N. Dose translation from animal to human studies revisited[J]. FASEB J, 2008, 22(3):659-661.
11. 何成奇, 王維, 肖登, 等. 不同頻率脈沖電磁場對去勢大鼠血清雌二醇及股骨骨鈣含量的影響[J]. 中國康復醫學雜志, 2007, 22(4):303-305.
12. Abbott TA 3rd, Mucha L, Manfredonia D, et al. Efficient patient identification strategies for women with osteoporosis[J]. J Clin Densitom, 1999, 2(3):223-230.
13. van't Hof RJ, Ralston SH. Nitric oxide and bone[J]. Immunology, 2001, 103(3):255-161.
14. 丁桂芝, 李仁康, 李榕, 等. 從骨礦含量變化規律看腎主骨理論的科學性[J]. 湖北中醫雜志, 1991, 13(2):27-29.
15. 李麗靜, 王喜軍, 王曉麗. 納米三鹿方對骨質疏松大鼠骨密度及鈣磷含量的影響[J]. 現代中醫藥, 2006, 26(3):54-56.
16. 國家藥典委員會. 中華人民共和國藥典[J]. 北京:化學工業出版社, 2009:79-82.
17. 齊艷萍. 鹿茸的化學成分及對肝損傷的修復作用[J]. 甘肅中醫, 2010, 23(1):61-63.
18. 王守本, 孫德水, 周淑榮. 鹿血與鹿茸血的研究利用概況[J]. 特產研究, 1999(1):52-57.
19. Kishi S, Segawa Y, Yamaquchi M. Histomorphological confirmation of the preventive effect of beta-alanyl-L-histidinato zinc on bone loss in ovariectomized rats[J]. Biol Pharm Bull, 1994, 17(6):862-865.
20. Pacifici R. T cells:critical bone regulators in health and disease[J]. Bone, 2010, 47(3):461-471.
21. Nomura K, Kuroda S, Yoshikawa H, et al. Inflammatory osteoclast genesis can be induced by GM-CSF and activated under TNF immunity[J]. Biochem Biophs Res Commun, 2008, 367(4):881-887.
22. 董萬超, 劉春華, 趙立波, 等. 馬鹿茸、梅花鹿茸不同部位無機元素含量測定分析[J]. 特產研究, 2004, 26(3):32-36.
23. 邱學敏, 王凌, 李大金.調節性細胞對破骨細胞的調控作用及其機制[J]. 現代免疫學,2013,33(1):71-73.

West China Medical Journal

Effects of Pilose Antler Blood Wine on Bone Mineral Density and T-lymphocyte Subsets in Ovariectomized Female Rats

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