Optimization of the Extracting Process of Zipu Gouty Mixture by Orthogonal Design_West China Medical Journal

Authors：

 LiuZhongjun ¹ ,  FengGuangfu ¹ , PiFengjuan ² , TuHe ¹ , ZhuLiyang ¹ , PangChaoping ¹

1. Department of Pharmacy, the Orthopedic Hospital of Sichuan Province, Chengdu, Sichuan 610041, P. R. China;
2. Department of Pharmacy, Luzhou Hospital of Traditional Chinese Medicine, Luzhou, Sichuan 646000, P. R. China;

Corresponding?author：

FengGuangfu, Email: 465197979@qq.com

Keywords：

Zipu gouty mixture; Orthogonal design; Extracting process; Chlorogenic acid

DOI：

10.7507/1002-0179.201600466

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To optimize the extracting process of Zipu gouty mixture by orthogonal experiment, and to provide theoretical basis for its preparation procedure and quality control. Methods The water drawn extract yield and the total content of the chlorogenic acid were considered as research indexes. The orthogonal test was conducted to evaluate the effects of three factors including the amount of material/solvent ratio, extraction times, and duration of sample extraction of Zipu gouty mixture extracting process. Results The extracting process was optimized with the material/solvent ratio of 1:8, extract time of 30 minutes for 3 cycles. Conclusion The Zipu gouty mixture extracting process optimized by orthogonal test is simple, reliable and easy to repeat, which provides a theoretical basis for large-scale production.

Citation： LiuZhongjun, FengGuangfu, PiFengjuan, TuHe, ZhuLiyang, PangChaoping. Optimization of the Extracting Process of Zipu Gouty Mixture by Orthogonal Design. West China Medical Journal, 2016, 31(10): 1693-1696. doi: 10.7507/1002-0179.201600466 Copy

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1. Nan H, Qiao Q, Dong Y, et al. The prevalence of hyperuricemia in a population of the coastal city of Qingdao, China[J]. J Rheumatol, 2006, 33(7): 1346-1350.
2. 李昕雪.中藥治療痛風的系統綜述和方法學研究[D].北京:北京中醫藥大學, 2013.
3. 左霞, 劉春霞, 楊金霞, 等. HPLC法測定不同來源不同采收期忍冬藤和金銀花中綠原酸含量[J].中國藥師, 2015(4): 682-684.
4. 熊茉君, 毛興榮, 張敏, 等. HPLC測定柔肝解毒膠囊中的綠原酸[J].華西藥學雜志, 2010, 25(6): 746-747.
5. 許沛虎, 高媛, 張雪瓊, 等.正交實驗法優選黃連生物堿提取工藝[J].醫藥導報, 2008, 27(4): 446-448.
6. 李根, 王桂蓉, 伍麗萍, 等.正交試驗優選紫冰栓中紫草油提取工藝的研究[J].華西醫學, 2010, 25(12): 2228-2230.
7. 史玉紅.梔子藥材中梔子苷和綠原酸含量測定方法研究[J].遼寧中醫藥大學學報, 2013, 15(6): 38-40.
8. 國家藥典委員會.中華人民共和國藥典-一部[M].北京:化學工業出版社, 2005: 286.
9. 潘新波, 李彩東, 王信, 等.正交試驗法優選金茵利膽口服液水提工藝[J].中國藥師, 2015, 18(4): 585-588.
10. 文卓瓊, 蔣利華, 熊海蓉, 等.微波法提取石蒜中石蒜堿的工藝優化[J].中國農學通報, 2015, 31(24): 247-251.
11. 劉穎, 郭明曄, 白根本.綠原酸的研究進展[J].中藥材, 2012, 35(7): 1180-1185.
12. 趙君峰, 白志川, 劉世堯.綠原酸提取及分析測定方法研究進展[J].遼寧中醫藥大學學報, 2007, 9(6): 80-82.
13. 余晟嵐, 徐果.反相高效液相色譜法測定金嗓口服液中綠原酸含量[J].中國藥業, 2015(10): 65-66.
14. 鄧松岳, 王迎舉. HPLC法測定抗毒合劑中綠原酸的含量[J].中國中醫藥現代遠程教育, 2015, 13(9): 131-133.
15. 孟姝, 林玉蓮, 都述虎. HPLC同時測定利膽片中綠原酸和黃芩苷的含量[J].中國實驗方劑學雜志, 2015, 21(10): 77-79.

West China Medical Journal

Optimization of the Extracting Process of Zipu Gouty Mixture by Orthogonal Design

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