健脾化濕法對特應性皮炎小鼠皮膚水通道蛋白3表達的影響_West China Medical Journal

Authors：

 周愛妍 ¹ ,  杜迎 ² , 史傳奎 ¹

1. ;
2. ;

Corresponding?author：

杜迎, Email: duying0813@126.com

Keywords：

特應性皮炎; 健脾化濕法; 水通道蛋白

DOI：

10.7507/1002-0179.20160033

Video：

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目的觀察健脾化濕法對特應性皮炎（AD）小鼠水通道蛋白3（AQP3）表達的影響，探討健脾化濕法治療AD的作用機制與途徑，為進一步闡明健脾化濕法治療AD的分子生物學提供依據。方法將30只7周齡昆明小鼠隨機分為中藥模型組、空白模型組、空白對照組，每組各10只。中藥模型組和空白模型組建立皮炎模型，建模后中藥模型組采用小兒健脾化濕水煎液灌胃，劑量為10 mL/kg，2次/d，空白模型組以等量生理鹽水灌胃；空白對照組不建立皮炎模型，以等量生理鹽水灌胃。小鼠鼠齡達11周齡同時取其背部皮膚組織采用免疫組織化學法（PV法）檢測AD小鼠皮膚AQP3的表達及分布。結果中藥模型組、空白模型組、空白對照組小鼠皮膚組織中AQP3吸光度值分別為0.450±0.061、0.611±0.063、0.261±0.040，空白模型組高于空白對照組，差異有統計學意義（P＜0.01）；中藥模型組低于空白模型組，差異有統計學意義（P＜0.01）。結論健脾化濕法可以明顯改善AD小鼠皮膚組織中AQP3的表達與分布，促進皮膚組織的修復。

Citation： 周愛妍, 杜迎, 史傳奎. 健脾化濕法對特應性皮炎小鼠皮膚水通道蛋白3表達的影響. West China Medical Journal, 2016, 31(1): 119-121. doi: 10.7507/1002-0179.20160033 Copy

1.	陳保疆, 張玉環. 健脾利濕湯治療異位性皮炎的療效觀察與相關實驗研究[J]. 吉林中醫藥, 2011, 31(4):335-336.
2.	石漢振, 曾冬玉, 陳達燦, 等. 健脾滲濕法治療特應性皮炎患者血清總IgE、IgG、IgG4變化及療效評價[J]. 中國中西醫結合皮膚性病學雜志, 2011, 10(1):20-22.
3.	譚遠忠, 魏文斌, 劉華, 等. 運脾化濕中藥對腸易激綜合征脾胃濕阻證患者AQP8表達的影響研究[J]. 中藥材, 2009, 32(9):1487-1490.
4.	宋秀祖, 宋為民, 許愛娥, 等. AQP3、Caspase14、博來霉素水解酶對慢性濕疹皮膚屏障的作用研究[J]. 中華皮膚科雜志, 2012, 45(2):83-86.
5.	Pokharel YR, Lim SC, Kim SC, et al. Sopungyangiac-Tang inhibits development of dermatitis in Nc/Nga mice[J]. Evid Based Complement Altemat Med, 2008, 5(2):173-180.
6.	Park CW, Youn M, Jung YM, et al. New functional probiotic Lactobacillus sakei probio 65 alleviates atopic symptoms in the mouse[J]. J Med Food, 2008, 11(3):405-412.
7.	Qi XF, Kim DH, Yoon YS, et al. Effects of bambusae caulis in liquamen on the development of atopic dermatitis-like skin lesions in hairless mice[J]. Ethnopharmaeol, 2009, 123(2):195-200.
8.	陳奇. 中藥藥理研究方法學[M]. 2版. 北京:人民衛生出版社, 2006:31.
9.	李輝, 高強, 王翼騰, 等. 應用小鼠建立特應性皮炎模型的研究進展[J]. 武警醫學, 2015, 26(1):99-102.
10.	Wang KS, Ma TH, Filiz F, et al. Colon water transport in transgenic mice lacking aquaporin-4 water channels[J]. Am J Physiol Gastrointest Liver Physiol, 2000, 279(2):G463-G470.
11.	Toshiyuki, Takeshi S. Water Channel protein AQP3 is present in epithelia exposed to the environment of possible water loss[J]. Histochem Soc, 1999, 47(10):1275-1286.
12.	Agren J. Transepidermal water loss in developing rats:role of aquaporins in the immature skin[J]. J Pediatr Res, 2003, 53(4): 558-565.
13.	Warner RR, Myers MC, Taylor DA. Electron probe analysis of human skin:determination of the water concentration profile[J]. J Invest Dermatol, 1988, 90:218-224.
14.	Olsson M, Broberg A, Jern?s M, et al. Increased expression of aquaporin 3 in atopic eczema[J]. Allergy, 2006, 61(9):1132-1137.

1. 陳保疆, 張玉環. 健脾利濕湯治療異位性皮炎的療效觀察與相關實驗研究[J]. 吉林中醫藥, 2011, 31(4):335-336.
2. 石漢振, 曾冬玉, 陳達燦, 等. 健脾滲濕法治療特應性皮炎患者血清總IgE、IgG、IgG4變化及療效評價[J]. 中國中西醫結合皮膚性病學雜志, 2011, 10(1):20-22.
3. 譚遠忠, 魏文斌, 劉華, 等. 運脾化濕中藥對腸易激綜合征脾胃濕阻證患者AQP8表達的影響研究[J]. 中藥材, 2009, 32(9):1487-1490.
4. 宋秀祖, 宋為民, 許愛娥, 等. AQP3、Caspase14、博來霉素水解酶對慢性濕疹皮膚屏障的作用研究[J]. 中華皮膚科雜志, 2012, 45(2):83-86.
5. Pokharel YR, Lim SC, Kim SC, et al. Sopungyangiac-Tang inhibits development of dermatitis in Nc/Nga mice[J]. Evid Based Complement Altemat Med, 2008, 5(2):173-180.
6. Park CW, Youn M, Jung YM, et al. New functional probiotic Lactobacillus sakei probio 65 alleviates atopic symptoms in the mouse[J]. J Med Food, 2008, 11(3):405-412.
7. Qi XF, Kim DH, Yoon YS, et al. Effects of bambusae caulis in liquamen on the development of atopic dermatitis-like skin lesions in hairless mice[J]. Ethnopharmaeol, 2009, 123(2):195-200.
8. 陳奇. 中藥藥理研究方法學[M]. 2版. 北京:人民衛生出版社, 2006:31.
9. 李輝, 高強, 王翼騰, 等. 應用小鼠建立特應性皮炎模型的研究進展[J]. 武警醫學, 2015, 26(1):99-102.
10. Wang KS, Ma TH, Filiz F, et al. Colon water transport in transgenic mice lacking aquaporin-4 water channels[J]. Am J Physiol Gastrointest Liver Physiol, 2000, 279(2):G463-G470.
11. Toshiyuki, Takeshi S. Water Channel protein AQP3 is present in epithelia exposed to the environment of possible water loss[J]. Histochem Soc, 1999, 47(10):1275-1286.
12. Agren J. Transepidermal water loss in developing rats:role of aquaporins in the immature skin[J]. J Pediatr Res, 2003, 53(4): 558-565.
13. Warner RR, Myers MC, Taylor DA. Electron probe analysis of human skin:determination of the water concentration profile[J]. J Invest Dermatol, 1988, 90:218-224.
14. Olsson M, Broberg A, Jern?s M, et al. Increased expression of aquaporin 3 in atopic eczema[J]. Allergy, 2006, 61(9):1132-1137.

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West China Medical Journal

健脾化濕法對特應性皮炎小鼠皮膚水通道蛋白3表達的影響

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