Application of Chronic Unpredictable Mild Stress Female Kunming Mice Model in the Field of Reproductive Medicine_West China Medical Journal

Authors：

 LIXiao-hong , LIShang-wei , LUOShan , FANWei , ZENGXun , BAIYu ,  QINLang

Reproductive Medicine Center, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding?author：

QINLang, Email: cacier@126.com

Keywords：

Chronic unpredictable mild stress; Rreproduction; Kunming mice; Open field test

DOI：

10.7507/1002-0179.20150587

Video：

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Objective To explore the effect of chronic unpredictable mild stress (CUMS) on the reproductive function of mice and provide a suitable animal model for reproduction and stress. Methods A total of 240 female Kunming mice were feed for 5 days, and then divided randomly into the control group (n=90) and experimental group (n=150). The mice in the experimental group were stressed by 9 chronic mild unpredictable stress factors for 4 weeks and validated by open field test and sucrose consumption test. We administrated pregnant mare serum gonadotropin (PMSG)/human chorionic gonadotropin (HCG) for induction of superovulation and observed the ovarian response and embryo development potential. Results After 4-week CUMS stimulation, the weight gain, 2% sugar consumption test and open field test were significantly different between the mice in two groups (P>0.05). After PMSG/HCG was administrated, the antra follicles and preovulatory follicles significantly reduced significantly in the experiment group than that in the control group (P<0.05); the number of oocytes, fertilization rate, 2-cell embryos, D4 embryos, blastocysts, high quality embryo rate and D5 bed points were all significantly decreased in the experiment group than those in the control group (P<0.05). Conclusion The CUMS female Kunming mice model is a kind of emotional stress animal model with low reproductive function, which is effective, operable and repeatable; it could be used for further study on the mechanism of reproductive medicine.

Citation： LIXiao-hong, LIShang-wei, LUOShan, FANWei, ZENGXun, BAIYu, QINLang. Application of Chronic Unpredictable Mild Stress Female Kunming Mice Model in the Field of Reproductive Medicine. West China Medical Journal, 2015, 30(11): 2071-2075. doi: 10.7507/1002-0179.20150587 Copy

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2.	Lund R, Sejbaek CS, Christensen U, et al. The impact of social relations on the incidence of severe depressive symptoms among infertile women and men[J]. Hum Reprod, 2009, 24(11):2810-2820.
3.	Sydsj? G, Svanberg AS, Lampic C, et al. Relationships in IVF couples 20 years after treatment[J]. Hum Reprod, 2011, 26(7):1836-1842.
4.	Li XH, Ma YG, Geng LH, et al. Baseline psychological stress and ovarian norepinephrine levels negatively affect the outcome of in vitro fertilisation[J]. Gynecol Endocrinol, 2011, 27(3):139-143.
5.	張臣顥, 張曼芳, 謝青蓮, 等. 慢性不可預知溫和應激抑郁小鼠模型的建立[J]. 復旦學報:自然科學版, 2011, 5(3):390-393.
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7.	Guo JY, Bian H, Yao Y. Chronic unpredictable mild stress induces parallel reductions of 15-PGDH in the hypothalamus and lungs in rats[J]. Behav Brain Res, 2015, 286(286):278-284.
8.	Yazir Y, Utkan T, Gacar N, et al. Resveratrol exerts anti-inflammatory and neuroprotective effects to prevent memory deficits in rats exposed to chronic unpredictable mild stress[J]. Physiol Behav, 2015, 138:297-304.
9.	Franceschelli A, Herchick S, Thelen C, et al. Sex differences in the chronic mild stress model of depression[J]. Behav Pharmacol, 2014, 25(5/6):372-383.
10.	王海濤, 薛黎明, 秦雪梅, 等. 慢性不可預知和慢性束縛應激抑郁模型大鼠行為學研究[J]. 山西大學學報:自然科學版, 2010, 33(1):157-160.
11.	Ebbesen SM, Zachariae R, Mehlsen MY, et al. Stressful life events are associated with a poor in-vitro fertilization (IVF) outcome:a prospective study[J]. Hum Reprod, 2009, 24(9):2173-2182.
12.	Bleil ME, Adler NE, Pasch LA, et al. Psychological stress and reproductive aging among pre-menopausal women[J]. Hum Reprod, 2012, 27(9):2720-2728.
13.	Bleil ME, Adler NE, Pasch LA, et al. Depressive symptomatology, psychological stress, and ovarian reserve:a role for psychological factors in ovarian aging?[J]. Menopause, 2012, 19(11):1176-1185.
14.	Cizmeli C, Lobel M, Franasiak J, et al. Levels and associations among self-esteem, fertility distress, coping, and reaction to potentially being a genetic carrier in women with diminished ovarian reserve[J]. Fertil Steril, 2013, 99(7):2037-2044.
15.	Matthiesen SM, Frederiksen Y, Ingerslev H, et al. Stress, distress and outcome of assisted reproductive technology (ART):a meta-analysis[J]. Hum Reprod, 2011, 26(10):2763-2776.
16.	Quant HS, Zapantis A, Nihsen M, et al. Reproductive implications of psychological distress for couples undergoing IVF[J]. J Assist Reprod Genet, 2013, 30(11):1451-1458.
17.	An Y, Sun Z, Li L, et al. Relationship between psychological stress and reproductive outcome in women undergoing in vitro fertilization treatment:psychological and neurohormonal assessment[J]. J Assist Reprod Genet, 2013, 30(1):35-41.
18.	娜琴, 程飚, 陳阿梅. 應激動物模型構建方法及效果評價的研究進展[J]. 中國組織工程研究與臨床康復, 2011, 15(11):2051-2054.
19.	廖莎, 周佳, 平鋒鋒, 等. 慢性束縛與慢性不可預期溫和應激抑郁模型小鼠的行為學比較及其發生機制研究[J]. 西北農林科技大學學報:自然科學版, 2012, 40(11):29-34, 41.
20.	程湘, 徐輝, 艾國平, 等. 慢性情緒應激對大鼠卵巢功能影響的初步觀察[J]. 第三軍醫大學學報, 2007, 29(14):1368-1370.
21.	Wu LM, Liu YS, Tong XH, et al. Inhibition of follicular development induced by chronic unpredictable stress is associated with growth and differentiation factor 9 and gonadotropin in mice[J]. Biol Reprod, 2012, 86(4):121.

1. Peterson BD, Pirritano M, Christensen U, et al. The longitudinal impact of partner coping in couples following 5 years of unsuccessful fertility treatments[J]. Hum Reprod, 2009, 24(7):1656-1664.
2. Lund R, Sejbaek CS, Christensen U, et al. The impact of social relations on the incidence of severe depressive symptoms among infertile women and men[J]. Hum Reprod, 2009, 24(11):2810-2820.
3. Sydsj? G, Svanberg AS, Lampic C, et al. Relationships in IVF couples 20 years after treatment[J]. Hum Reprod, 2011, 26(7):1836-1842.
4. Li XH, Ma YG, Geng LH, et al. Baseline psychological stress and ovarian norepinephrine levels negatively affect the outcome of in vitro fertilisation[J]. Gynecol Endocrinol, 2011, 27(3):139-143.
5. 張臣顥, 張曼芳, 謝青蓮, 等. 慢性不可預知溫和應激抑郁小鼠模型的建立[J]. 復旦學報:自然科學版, 2011, 5(3):390-393.
6. Cai L, Li R, Tang WJ, et al. Antidepressant-like effect of geniposide on chronic unpredictable mild stress-induced depressive rats by regulating the hypothalamus-pituitary-adrenal axis[J]. Eur Neuropsychopharmacol, 2015, 25(8):1332-1341.
7. Guo JY, Bian H, Yao Y. Chronic unpredictable mild stress induces parallel reductions of 15-PGDH in the hypothalamus and lungs in rats[J]. Behav Brain Res, 2015, 286(286):278-284.
8. Yazir Y, Utkan T, Gacar N, et al. Resveratrol exerts anti-inflammatory and neuroprotective effects to prevent memory deficits in rats exposed to chronic unpredictable mild stress[J]. Physiol Behav, 2015, 138:297-304.
9. Franceschelli A, Herchick S, Thelen C, et al. Sex differences in the chronic mild stress model of depression[J]. Behav Pharmacol, 2014, 25(5/6):372-383.
10. 王海濤, 薛黎明, 秦雪梅, 等. 慢性不可預知和慢性束縛應激抑郁模型大鼠行為學研究[J]. 山西大學學報:自然科學版, 2010, 33(1):157-160.
11. Ebbesen SM, Zachariae R, Mehlsen MY, et al. Stressful life events are associated with a poor in-vitro fertilization (IVF) outcome:a prospective study[J]. Hum Reprod, 2009, 24(9):2173-2182.
12. Bleil ME, Adler NE, Pasch LA, et al. Psychological stress and reproductive aging among pre-menopausal women[J]. Hum Reprod, 2012, 27(9):2720-2728.
13. Bleil ME, Adler NE, Pasch LA, et al. Depressive symptomatology, psychological stress, and ovarian reserve:a role for psychological factors in ovarian aging?[J]. Menopause, 2012, 19(11):1176-1185.
14. Cizmeli C, Lobel M, Franasiak J, et al. Levels and associations among self-esteem, fertility distress, coping, and reaction to potentially being a genetic carrier in women with diminished ovarian reserve[J]. Fertil Steril, 2013, 99(7):2037-2044.
15. Matthiesen SM, Frederiksen Y, Ingerslev H, et al. Stress, distress and outcome of assisted reproductive technology (ART):a meta-analysis[J]. Hum Reprod, 2011, 26(10):2763-2776.
16. Quant HS, Zapantis A, Nihsen M, et al. Reproductive implications of psychological distress for couples undergoing IVF[J]. J Assist Reprod Genet, 2013, 30(11):1451-1458.
17. An Y, Sun Z, Li L, et al. Relationship between psychological stress and reproductive outcome in women undergoing in vitro fertilization treatment:psychological and neurohormonal assessment[J]. J Assist Reprod Genet, 2013, 30(1):35-41.
18. 娜琴, 程飚, 陳阿梅. 應激動物模型構建方法及效果評價的研究進展[J]. 中國組織工程研究與臨床康復, 2011, 15(11):2051-2054.
19. 廖莎, 周佳, 平鋒鋒, 等. 慢性束縛與慢性不可預期溫和應激抑郁模型小鼠的行為學比較及其發生機制研究[J]. 西北農林科技大學學報:自然科學版, 2012, 40(11):29-34, 41.
20. 程湘, 徐輝, 艾國平, 等. 慢性情緒應激對大鼠卵巢功能影響的初步觀察[J]. 第三軍醫大學學報, 2007, 29(14):1368-1370.
21. Wu LM, Liu YS, Tong XH, et al. Inhibition of follicular development induced by chronic unpredictable stress is associated with growth and differentiation factor 9 and gonadotropin in mice[J]. Biol Reprod, 2012, 86(4):121.

West China Medical Journal

Application of Chronic Unpredictable Mild Stress Female Kunming Mice Model in the Field of Reproductive Medicine

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