傷口細菌生物膜研究進展_West China Medical Journal

Authors：

 鄧欣 ^1,2 , 何凌霄 ³ , 陳佳麗 ¹ ,  寧寧 ¹

1. ;
2. ;
3. ;

Corresponding?author：

寧寧, Email: gkningning@126.com

Keywords：

慢性傷口; 傷口感染; 生物膜

DOI：

10.7507/1002-0179.20150529

Video：

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Abstract Full text Figures/Tables Video References Cited by

細菌生物膜對慢性傷口的影響和其治療近年來成為傷口護理領域的一大研究熱點，傷口生物膜的存在可能是造成慢性傷口難以愈合的重要原因，其具有復雜的內部結構和多種溝通交流機制，對理化刺激、免疫反應和抗菌藥物都具有很強的抗性，難以被徹底清除；傷口生物膜的治療應該是一整套管理方案，包括基本的傷口管理措施、定期清創和抗菌藥物的使用，以清除生物膜和防止其再生。慢性傷口細菌生物膜的存在值得重視，而現有文獻證據尚不能充分說明其作用機制以及何種治療方案有效，值得進一步進行研究。

Citation： 鄧欣, 何凌霄, 陳佳麗, 寧寧. 傷口細菌生物膜研究進展. West China Medical Journal, 2015, 30(10): 1838-1841. doi: 10.7507/1002-0179.20150529 Copy

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2.	Thomas DW, Harding KG. Wound healing[J]. Br J Surg 2002, 89(10):1203-1205.
3.	Percival SL, Hill KE, Williams DW, et al. A review of the scientific evidence for biofilms in wounds[J]. Wound Repair Regen, 2012, 20(5):647-657.
4.	Mustoe TA, O'Shaughnessy K, Kloeters O. Chronic wound pathogenesis and current treatment strategies:a unifying hypothesis[J]. Plast Reconstr Surg, 2006, 117(7 Suppl):35S-41S.
5.	Widgerow AD. Persistence of the chronic wound-implicating biofilm[J]. Wound Heal South Afr, 2008, 1(2):5-9.
6.	Phillips PL, Wolcott RD, Fletcher J, et al. Biofilms made easy[J]. Wounds Int, 2010, 1(3):1-6.
7.	Costerton JW, Lewandowski Z, Caldwell DE, et al. Microbial biofilms[J]. Annu Rev Microbiol, 1995, 49(1): 711-745.
8.	Donlan RM. Biofilms:microbial life on surfaces[J]. Emerg Infect Dis, 2002, 8(9):881-890.
9.	Stoodley P, Sauer K, Davies DG, et al. Biofilms as complex differentiated communities[J]. Annu Rev Microbiol, 2002, 56(1):187-209.
10.	Watnick P, Biofilm KR. City of microbes[J]. J Bacteriol, 2000, 182(10):2675-2679.
11.	Costerton W, Veeh R, Shirtliff M, et al. The application of biofilm science to the study and control of chronic bacterial infections[J]. J Clin Invest, 2003, 112(10):1466-1477.
12.	Wimpenny J, Manz W, Szewzyk U. Heterogeneity in biofilms[J]. FEMS Microbiol Rev, 2000, 24(5):661-671.
13.	Bowler PG. Wound pathophysiology, infection and therapeutic options[J]. Ann Med, 2002, 34(6):419-427.
14.	Davies CE, Hill KE, Wilson MJ, et al. Use of 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis for analysis of the microfloras of healing and nonhealing chronic venous leg ulcers[J]. J Clin Microbiol, 2004, 42(8):3549-3557.
15.	Davies CE, Hill KE, Newcombe RG, et al. A prospective study of the microbiology of chronic venous leg ulcers to reevaluate the clinical predictive value of tissue biopsies and swabs[J]. Wound Repair Regen, 2007, 15(1):17-22.
16.	James GA, Swogger E, Wolcott R, et al. Biofilms in chronic wounds[J]. Wound Repair Regen, 2008, 16(1):37-44.
17.	Ngo QD, Vickery K, Deva AK. PR21 Role of bacterial biofilms in chronic wounds[J]. ANZ J Surg, 2007, 77(S1):A66.
18.	Kirketerp-M?ller K, Bjarnsholt T, Thomsen TR. The fight against biofilm infections:do we have the knowledge and means?[J]. EWMA J, 2011, 11(2):8-9.
19.	Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms:a common cause of persistent infections[J]. Science, 1999, 284(5418):1318-1322.
20.	Phillips P, Sampson E, Yang Q, et al. Bacterial biofilms in wounds[J]. Wound Heal South Afr, 2009, 1(2):10-12.
21.	Fadeev SB, Nemtseva NV. Formation of biofilms by agents of surgical soft tissue infections[J]. Zh Mikrobiol Epidemiol Immunobiol, 2009(4):114-117.
22.	Flemming HC, Neu TR, Wozniak DJ. The EPS matrix:the "House of Biofilm cells"[J]. J Bacteriol, 2007, 189(22):7945-7947.
23.	Brooun A, Liu S, Lewis K. A dose-response study of antibiotic resistance in Pseudomonas aeruginosa biofilms[J]. Antimicrob Agents Chemother, 2000, 44(3):640-646.
24.	Olson ME, Ceri H, Morck DW, et al. Biofilm bacteria:formation and comparative susceptibility to antibiotics[J]. Can J Vet Res, 2002, 66(2):86-92.
25.	Conley J, Olson ME, Cook LS, et al. Biofilm formation by group a streptococci:is there a relationship with treatment failure?[J].J Clin Microbiol, 2003, 41(9):4043-4048.
26.	Hall-Stoodley L, Stoodley P. Evolving concepts in biofilm infections[J]. Cell Microbiol, 2009, 11(7):1034-1043.
27.	Weigel LM, Donlan RM, Shin DH, et al. High-level vancomycin-resistant Staphylococcus aureus isolates associated with a polymicrobial biofilm[J]. Antimicrob Agents Chemother, 2007, 51(1):231-238.
28.	Miller MB, Bassler BL. Quorum sensing in bacteria[J]. Annu Rev Microbiol, 2001, 55(1):165-199.
29.	Bester E, Kroukamp O, Wolfaardt GM, et al. Metabolic differentiation in biofilms as indicated by carbon dioxide production rates[J]. Appl Environ Microbiol, 2010, 76(4):1189-1197.
30.	Wolcott RD, Rumbaugh KP, James G, et al. Biofilm maturity studies indicate sharp debridement opens a time-dependent therapeutic window[J]. J Wound Care, 2010, 19(8):320-328.
31.	Sun Y, Dowd SE, Smith E, et al. In vitro multispecies Lubbock chronic wound biofilm model[J]. Wound Repair Regen, 2009, 16(6):805-813.
32.	Davis SC, Ricotti C, Cazzaniga A, et al. Microscopic and physiologic evidence for biofilm-associated wound colonization in vivo[J]. Wound Repair Regen, 2008, 16(1):23-29.
33.	Cooper R. Biofilms:hard to detect, easy to underestimate, but most definitely here to stay[J]. Wounds UK J, 2009, 5(1):12.
34.	Wolcott RD, Rhoads DD. A study of biofilm-based wound management in subjects with critical limb ischaemia[J]. J Wound Care, 2008, 17(4):145-148, 150-152, 154-155.
35.	Wolcott RD, Kennedy JP, Dowd SE. Regular debridement is the main tool for maintaining a healthy wound bed in most chronic wounds[J]. J Wound Care, 2009, 18(2):54-56.
36.	Strohal R. The EWMA document:debridement[J]. J Wound Care, 2013, 22(1):5.
37.	Hill KE, Malic S, Mckee R, et al. An in vitro model of chronic wound biofilms to test wound dressings and assess antimicrobial susceptibilities[J]. J Antimicrob Chemother, 2010, 65(6):1195-1206.
38.	Percival SL, Bowler P, Woods EJ. Assessing the effect of an antimicrobial wound dressing on biofilms[J]. Wound Repair Regen, 2008, 16(1):52-57.
39.	Dowd SE, Sun Y, Smith E, et al. Effects of biofilm treatments on the multi-species Lubbock chronic wound biofilm model[J]. J Wound Care, 2009, 18(508):510-512.
40.	Bjarnsholt T, Kirketerp-M?ller K, Kristiansen S, et al. Silver against Pseudomonas aeruginosa biofilms[J]. APMIS, 2007, 115(8):921-928.
41.	Kim PJ, Steinberg JS. Wound care:biofilm and its impact on the latest treatment modalities for ulcerations of the diabetic foot[J]. Semin Vasc Surg, 2012, 25(2):70-74.

1. Gottrup F. A specialized wound-healing center concept:importance of a multidisciplinary department structure and surgical treatment facilities in the treatment of chronic wounds[J]. Am J Surg, 2004, 187(5A):38S-43S.
2. Thomas DW, Harding KG. Wound healing[J]. Br J Surg 2002, 89(10):1203-1205.
3. Percival SL, Hill KE, Williams DW, et al. A review of the scientific evidence for biofilms in wounds[J]. Wound Repair Regen, 2012, 20(5):647-657.
4. Mustoe TA, O'Shaughnessy K, Kloeters O. Chronic wound pathogenesis and current treatment strategies:a unifying hypothesis[J]. Plast Reconstr Surg, 2006, 117(7 Suppl):35S-41S.
5. Widgerow AD. Persistence of the chronic wound-implicating biofilm[J]. Wound Heal South Afr, 2008, 1(2):5-9.
6. Phillips PL, Wolcott RD, Fletcher J, et al. Biofilms made easy[J]. Wounds Int, 2010, 1(3):1-6.
7. Costerton JW, Lewandowski Z, Caldwell DE, et al. Microbial biofilms[J]. Annu Rev Microbiol, 1995, 49(1): 711-745.
8. Donlan RM. Biofilms:microbial life on surfaces[J]. Emerg Infect Dis, 2002, 8(9):881-890.
9. Stoodley P, Sauer K, Davies DG, et al. Biofilms as complex differentiated communities[J]. Annu Rev Microbiol, 2002, 56(1):187-209.
10. Watnick P, Biofilm KR. City of microbes[J]. J Bacteriol, 2000, 182(10):2675-2679.
11. Costerton W, Veeh R, Shirtliff M, et al. The application of biofilm science to the study and control of chronic bacterial infections[J]. J Clin Invest, 2003, 112(10):1466-1477.
12. Wimpenny J, Manz W, Szewzyk U. Heterogeneity in biofilms[J]. FEMS Microbiol Rev, 2000, 24(5):661-671.
13. Bowler PG. Wound pathophysiology, infection and therapeutic options[J]. Ann Med, 2002, 34(6):419-427.
14. Davies CE, Hill KE, Wilson MJ, et al. Use of 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis for analysis of the microfloras of healing and nonhealing chronic venous leg ulcers[J]. J Clin Microbiol, 2004, 42(8):3549-3557.
15. Davies CE, Hill KE, Newcombe RG, et al. A prospective study of the microbiology of chronic venous leg ulcers to reevaluate the clinical predictive value of tissue biopsies and swabs[J]. Wound Repair Regen, 2007, 15(1):17-22.
16. James GA, Swogger E, Wolcott R, et al. Biofilms in chronic wounds[J]. Wound Repair Regen, 2008, 16(1):37-44.
17. Ngo QD, Vickery K, Deva AK. PR21 Role of bacterial biofilms in chronic wounds[J]. ANZ J Surg, 2007, 77(S1):A66.
18. Kirketerp-M?ller K, Bjarnsholt T, Thomsen TR. The fight against biofilm infections:do we have the knowledge and means?[J]. EWMA J, 2011, 11(2):8-9.
19. Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms:a common cause of persistent infections[J]. Science, 1999, 284(5418):1318-1322.
20. Phillips P, Sampson E, Yang Q, et al. Bacterial biofilms in wounds[J]. Wound Heal South Afr, 2009, 1(2):10-12.
21. Fadeev SB, Nemtseva NV. Formation of biofilms by agents of surgical soft tissue infections[J]. Zh Mikrobiol Epidemiol Immunobiol, 2009(4):114-117.
22. Flemming HC, Neu TR, Wozniak DJ. The EPS matrix:the "House of Biofilm cells"[J]. J Bacteriol, 2007, 189(22):7945-7947.
23. Brooun A, Liu S, Lewis K. A dose-response study of antibiotic resistance in Pseudomonas aeruginosa biofilms[J]. Antimicrob Agents Chemother, 2000, 44(3):640-646.
24. Olson ME, Ceri H, Morck DW, et al. Biofilm bacteria:formation and comparative susceptibility to antibiotics[J]. Can J Vet Res, 2002, 66(2):86-92.
25. Conley J, Olson ME, Cook LS, et al. Biofilm formation by group a streptococci:is there a relationship with treatment failure?[J].J Clin Microbiol, 2003, 41(9):4043-4048.
26. Hall-Stoodley L, Stoodley P. Evolving concepts in biofilm infections[J]. Cell Microbiol, 2009, 11(7):1034-1043.
27. Weigel LM, Donlan RM, Shin DH, et al. High-level vancomycin-resistant Staphylococcus aureus isolates associated with a polymicrobial biofilm[J]. Antimicrob Agents Chemother, 2007, 51(1):231-238.
28. Miller MB, Bassler BL. Quorum sensing in bacteria[J]. Annu Rev Microbiol, 2001, 55(1):165-199.
29. Bester E, Kroukamp O, Wolfaardt GM, et al. Metabolic differentiation in biofilms as indicated by carbon dioxide production rates[J]. Appl Environ Microbiol, 2010, 76(4):1189-1197.
30. Wolcott RD, Rumbaugh KP, James G, et al. Biofilm maturity studies indicate sharp debridement opens a time-dependent therapeutic window[J]. J Wound Care, 2010, 19(8):320-328.
31. Sun Y, Dowd SE, Smith E, et al. In vitro multispecies Lubbock chronic wound biofilm model[J]. Wound Repair Regen, 2009, 16(6):805-813.
32. Davis SC, Ricotti C, Cazzaniga A, et al. Microscopic and physiologic evidence for biofilm-associated wound colonization in vivo[J]. Wound Repair Regen, 2008, 16(1):23-29.
33. Cooper R. Biofilms:hard to detect, easy to underestimate, but most definitely here to stay[J]. Wounds UK J, 2009, 5(1):12.
34. Wolcott RD, Rhoads DD. A study of biofilm-based wound management in subjects with critical limb ischaemia[J]. J Wound Care, 2008, 17(4):145-148, 150-152, 154-155.
35. Wolcott RD, Kennedy JP, Dowd SE. Regular debridement is the main tool for maintaining a healthy wound bed in most chronic wounds[J]. J Wound Care, 2009, 18(2):54-56.
36. Strohal R. The EWMA document:debridement[J]. J Wound Care, 2013, 22(1):5.
37. Hill KE, Malic S, Mckee R, et al. An in vitro model of chronic wound biofilms to test wound dressings and assess antimicrobial susceptibilities[J]. J Antimicrob Chemother, 2010, 65(6):1195-1206.
38. Percival SL, Bowler P, Woods EJ. Assessing the effect of an antimicrobial wound dressing on biofilms[J]. Wound Repair Regen, 2008, 16(1):52-57.
39. Dowd SE, Sun Y, Smith E, et al. Effects of biofilm treatments on the multi-species Lubbock chronic wound biofilm model[J]. J Wound Care, 2009, 18(508):510-512.
40. Bjarnsholt T, Kirketerp-M?ller K, Kristiansen S, et al. Silver against Pseudomonas aeruginosa biofilms[J]. APMIS, 2007, 115(8):921-928.
41. Kim PJ, Steinberg JS. Wound care:biofilm and its impact on the latest treatment modalities for ulcerations of the diabetic foot[J]. Semin Vasc Surg, 2012, 25(2):70-74.

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傷口細菌生物膜研究進展

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