The Effect of Rotenone on Dopaminic Neurons of Brain in Rat Model of Parkinson's Disease_West China Medical Journal

Authors：

 ZHANGYan-ping , LIYan-gai , XUXiao-chen , WANGYing-jie

Department of Neurologic Medicine, the First People's Hospital of Handan City, Handan, Hebei 056002, P. R. China;

Corresponding?author：

Keywords：

Rotenone; Parkinson's disease; Dopamine; Oxidative stress; Malondialdehyde; Glutathione

DOI：

10.7507/1002-0179.20150406

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Objective To study the effect of rotenone on rat substantia nigra dopamine (DA) in the nervous system and oxidative stress parameters (malondialdehyde and glutathione), the influence of rotenone on DA neurons toxic effect and its pathogenesis. Methods This study applied back subcutaneous injection of rotenone in rats [1.0 mg/(kg·d)], and used immunocytochemistry technique to detect changes in the expression of tyrosine kinase (TH) in 10 rats of the control group and 10 rats of the experimental group. Spectrophotometry was used to detect the change of oxidative stress parameters in rats (malondialdehyde and glutathione). Results DA neurons in rats had various degrees of damage. The TH immune response strength of rats in the substantia nigra and striatum decreased significantly. The number of immune response nigra TH positive neurons was significantly less in the experimental group than in the control group (P< 0.01). Spectrophotometer method was used to detect the midbrain nigra of glutathione, which was significantly less in the experimental group than in the control group (P<0.01). Malondialdehyde in the experimental group was significantly higher (P<0.01). Conclusion Rotenone has obvious neurotoxicity, and can lead to the damage of DA neurons and obvious oxidative stress injury in rats, which provides an experimental basis for the pathogenesis of Parkinson's disease, and at the same time provides new targets for the treatment.

Citation： ZHANGYan-ping, LIYan-gai, XUXiao-chen, WANGYing-jie. The Effect of Rotenone on Dopaminic Neurons of Brain in Rat Model of Parkinson's Disease. West China Medical Journal, 2015, 30(8): 1407-1410. doi: 10.7507/1002-0179.20150406 Copy

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9.	Li YQ, Li H, Kaneko T, et al. Morphological features and electrophysiological properties of serotonergic and non-serotonergic projection neurons in the dorsal raphe nucleus. An intracellular recording and labeling study in rat brain slices[J]. Brain Res, 2001, 900(1):110-118.
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11.	Cummings JL, Masterman DL. Depression in patients with Parkinson's disease[J]. Int J Geriatr Psychiatry, 1999, 14(9):711-718.
12.	Heinz A, Ragan P, Jones DW, et al. Reduced central serotonin transporters in alcoholism[J]. Am J Psychiatry, 1998, 155(11):1544-1549.
13.	楊詠梅, 聶亞雄, 唐小卿. 魚藤酮對多巴胺能神經元的損傷作用及機制[J]. 國際病理科學與臨床雜志, 2006, 26(2):145-148.
14.	Dexter DT, Carter CJ, Wells FR, et al. Basal lipid peroxidation in substantia nigra is increased in Parkinson's disease[J]. J Neurochem, 1989, 52(2):381-389.
15.	Gorell JM, Johnson CC, Rybicki BA, et al. The risk of Parkinson's disease with exposure to pesticides, farming, well water, and rural living[J]. Neurology, 1998, 50(5):1346-1350.

1. 褚玉霞, 劉健, 馮潔, 等. 帕金森病大鼠中縫背核5-羥色胺能神經元放電頻率和放電形式的變化[J]. 生理學報, 2004, 56(5):597-602.
2. Majczyński H, Maleszak K, Cabaj A, et al. Serotonin-related enhancement of recovery of hind limb motor functions in spinal rats after grafting of embryonic raphe nuclei[J]. J Neurotrauma, 2005, 22(5):590-604.
3. 吳少平. 酪氨酸羥化酶及其活性片段在帕金森病中的作用[D]. 北京:中國人民解放軍軍事醫學科學院, 2006:7-8.
4. Eberle-Wang K, Lucki I, Chesselet MF. A role for the subthalamic nucleus in 5-HT_2C-induced oral dyskinesia[J]. Neuroscience, 1996, 72(1):117-128.
5. Shulman LM, Taback RL, Rabinstein AA, et al. Non-recognition of depression and other non-motor symptoms in Parkinson's disease[J]. Parkinsonism Relat Disord, 2002, 8(3):193-197.
6. 石葛明, 樊平, 崔慧先, 等. 帕金森癥患者腦標本5羥色胺轉運體的表達[J]. 基礎醫學與臨床, 2007, 27(3):263-266.
7. 黃超培, 趙鵬, 魚藤酮的神經毒性研究進展[J]. 國外醫學·衛生學分冊, 2005, 32(6):361-365.
8. Nielsen K, Brask D, Knudsen GM, et al. Immunodetection of the serotonin transporter protein is a more valid marker for serotonergic fibers than serotonin[J]. Synapse, 2006, 59(5):270-276.
9. Li YQ, Li H, Kaneko T, et al. Morphological features and electrophysiological properties of serotonergic and non-serotonergic projection neurons in the dorsal raphe nucleus. An intracellular recording and labeling study in rat brain slices[J]. Brain Res, 2001, 900(1):110-118.
10. Mori S, Takino T, Yamada H, et al. Immunohistochemical demonstration of serotonin nerve fibers in the subthalamic nucleus of the rat, cat and monkey[J]. Neurosci Lett, 1985, 62(3):305-309.
11. Cummings JL, Masterman DL. Depression in patients with Parkinson's disease[J]. Int J Geriatr Psychiatry, 1999, 14(9):711-718.
12. Heinz A, Ragan P, Jones DW, et al. Reduced central serotonin transporters in alcoholism[J]. Am J Psychiatry, 1998, 155(11):1544-1549.
13. 楊詠梅, 聶亞雄, 唐小卿. 魚藤酮對多巴胺能神經元的損傷作用及機制[J]. 國際病理科學與臨床雜志, 2006, 26(2):145-148.
14. Dexter DT, Carter CJ, Wells FR, et al. Basal lipid peroxidation in substantia nigra is increased in Parkinson's disease[J]. J Neurochem, 1989, 52(2):381-389.
15. Gorell JM, Johnson CC, Rybicki BA, et al. The risk of Parkinson's disease with exposure to pesticides, farming, well water, and rural living[J]. Neurology, 1998, 50(5):1346-1350.

West China Medical Journal

The Effect of Rotenone on Dopaminic Neurons of Brain in Rat Model of Parkinson's Disease

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