Experimental Study of the Influence of the Validity Period of Glassware Packaging on Sterilizing Instrument inside the Bio-safety Cabinet_West China Medical Journal

Authors：

 LIUDian-zhong ^1,2 , JIANGJun-qiang ² , GUOShi-li ² , LIUYao ² ,  ZHENGLi-ge ²

1. Department of Clinical Laboratory, Affiliated Hospital of Stomatology;
2. Orofacial Reconstruction and Regeneration Laboratory;Luzhou Medical College, Luzhou, Sichuan 646000, P. R. China;

Corresponding?author：

ZHENGLi-ge, Email: 541148817@qq.com

Keywords：

Autoclaving; Validity period; Paper-plastic packaging; Glassware packaging

DOI：

10.7507/1002-0179.20150072

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To explore the validity period of oral sterilizing instruments in different packages inside the bio-safety cabinet. Methods Inside the bio-safety cabinet, we brought the autoclaved dental gadgets packed in glassware and paper-plastic into microbial sampling cultivation experiment, and made observation of the bacteria growth at the 4th, 8th, 24th hour after sterilizing, with the sampling of once per 24 hours, lasting 10 days. Samples taken from the gadgets in paper-plastic each time when samples were taken from the gadgets in glassware were as the control. Results Under the condition that the bio-safety cabinet was not contaminated, no colony grew in neither group in 144 hours; 168 hours later, the cultivation results indicated that colonies started to grow（P<0.05）. Conclusion Under the condition that the bio-safety cabinet is pollution-free, the validity period of gadgets in glassware packaging for dental clinic practices is far longer than the 4-hour limit after the sterilized packages are opened, as is stipulated in the "Operating Specifications for Sterilization Technology on Dental Instruments of Medical Institutions".

Citation： LIUDian-zhong, JIANGJun-qiang, GUOShi-li, LIUYao, ZHENGLi-ge. Experimental Study of the Influence of the Validity Period of Glassware Packaging on Sterilizing Instrument inside the Bio-safety Cabinet. West China Medical Journal, 2015, 30(2): 233-235. doi: 10.7507/1002-0179.20150072 Copy

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12.	王春燕. 口腔科診療器械消毒滅菌存在的問題及對策[J]. 中華醫院感染學雜志, 2010, 20(1):67-68.
13.	Guggenheim B, Gander M, Koller MM. A comprehensive system for washing, pre-disinfecting and sterilizing of dental and surgical instruments[J]. Oral Health Prev Dent, 2004, 2(4):335-344.
14.	張昀, 張衛平, 吳穎, 等. 口腔診療器械消毒滅菌現狀的思考[J]. 甘肅醫藥, 2010, 29(5):580-582.
15.	中國國家標準化管理委員會管理委員會. GB 15982-2012醫院消毒衛生標準[S]. 2012.

1. 鄒詠梅, 汪鴻文, 汪志強, 等. 杭州市口腔診療機構消毒滅菌現狀[J]. 中國感染控制雜志, 2009, 8(3):192-194.
2. 王穎. 口腔專科醫院小器械的消毒滅菌[J]. 中國消毒學雜志, 2008, 25(2):220-221.
3. 中華人民共和國衛生部. 醫療機構口腔診療器械消毒技術操作規范[EB/OL]. (2005-03-06)[2014-01-01]. http://moh.gov.cn/yzygj/s3576/200804/1e3b43d1a3d442eb8457cddec01658c4.shtml.
4. Raju TB, Garapati S, Agrawal R, et al. Sterilizing Endodontic Files by four different sterilization methods to prevent cross-infection:an In-vitro study[J]. J Int Oral Health, 2013, 5(6):108-112.
5. Brusca MI, Mosca CO, Nastri N, et al. Long-term sterility of orthodontic-surgical appliances[J]. J Dent Res, 2003, 82(C):10.
6. 張素芹, 王琳, 韓春云. 紙塑袋包裝物品高壓滅菌后有效期分析[J]. 臨床醫學, 2010, 30(10):123-124.
7. 中華人民共和國衛生部. WS/T 367-2012醫療機構消毒技術規范[S]. 2012.
8. 中華人民共和國衛生部. WS/T 368-2012醫院空氣凈化管理規范[S]. 2012.
9. 余樹榮. 微生物學和微生物學檢驗[M]. 北京:人民衛生出版社, 1997:439-449.
10. 周庭銀, 趙虎. 臨床微生物學診斷與圖解[M]. 上海:上海科學技術出版社, 2001:15-400.
11. 趙佛容. 口腔護理學[M]. 上海:復旦大學出版社, 2004:173-174.
12. 王春燕. 口腔科診療器械消毒滅菌存在的問題及對策[J]. 中華醫院感染學雜志, 2010, 20(1):67-68.
13. Guggenheim B, Gander M, Koller MM. A comprehensive system for washing, pre-disinfecting and sterilizing of dental and surgical instruments[J]. Oral Health Prev Dent, 2004, 2(4):335-344.
14. 張昀, 張衛平, 吳穎, 等. 口腔診療器械消毒滅菌現狀的思考[J]. 甘肅醫藥, 2010, 29(5):580-582.
15. 中國國家標準化管理委員會管理委員會. GB 15982-2012醫院消毒衛生標準[S]. 2012.

West China Medical Journal

Experimental Study of the Influence of the Validity Period of Glassware Packaging on Sterilizing Instrument inside the Bio-safety Cabinet

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