Analysis of the Relationship between Fluorescence Patterns of Antinuclear Antibody and Antinuclear Antibody Profiles_West China Medical Journal

Authors：

 WANGLi ¹ , RENLi-ling ² , GAOYu ¹ , LIWen ¹ , LIShuo ³ ,  WUYong-kang ⁴

1. Department of Gynecology and Obstetrics, 452 Hospital of PLA, Chengdu, Sichuan 610061, P. R. China;
2. Department of Geriatrics Neurology, the Fifth Hospital of Chengdu, Chengdu, Sichuan 611130, P. R. China;
3. Department of Nutrition, 452 Hospital of PLA, Chengdu, Sichuan 610061, P. R. China;
4. Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, P. R. China;

Corresponding?author：

WUYong-kang, Email: vipwyk@163.com

Keywords：

Antibodies; antinuclear; Antinuclear antibody profiles; Autoantibody; Comparative study

DOI：

10.7507/1002-0179.20150023

Video：

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Objective To discuss the relationship between antinuclear antibody (ANA) fluorescence pattern detected by indirect immunity fluorescence (IIF) and antinuclear antibody profiles (including anti-dsDNA, RNP, Sm, SSa, SSb, Scl-70, Jo-1 and rib-P) in human serum. Methods A total of 7385 cases of ANA pattern and ANA profiles were retrospectively analyzed from January 2010 to December 2013. ANA was detected by IIF substrated as HEp-2 cells, anti-dsDNA by IIF substrated as crithidia, and the other 7 antibodies by enzyme immunoblot with purified antigen. Results Granular pattern mostly presented as anti-RNP, anti-Sm, anti-SSa and anti-SSb (P < 0.001); homogeneous pattern was anti-dsDNA and anti-SSa (P < 0.001); nucleolus, centromere, and mixed pattern was anti-SSa (P < 0.05); cytoplasm pattern was anti-rib-P and anti-SSa (P < 0.05). But few above antibodies could be detected in Golgi, dots, rim, actin, actotropomyosin, prolifevating cell nuclear antigen (PCNA) and vementin pattern. Homogeneous pattern was shown up to 77.91% in only anti-dsDNA positive serum; granular was 96.84%, 52.01%, and 82.35% respectively in only anti-RNP or anti-SSa or anti-Sm positive. Homogeneous and nucleolus mix pattern was up to 30.53% in only anti-Scl-70 positive. Cytoplasm pattern was 50.00% and 61.54% respectively in only anti-rib-P or anti-Jo-1 positive. But no fixed relationship was found between ANA pattern and anti-SSb. Conclusions There is a certain relationship between ANA and antinuclear antibody profiles. Granular, homogeneous and cytoplasm pattern often can be detected more than one autoantibodies. Eight kinds of specific autoantibodies often are negative when ANA patterns are centromere, Golgi, dots, rim, actin, tropomyosin, PCNA, and vimentin. Anti-dsDNA is mainly corresponding to homogeneous, anti-RNP, anti-SSa and anti-Sm to granular, anti-Scl-70 to homogeneous and nucleoli, anti-rib-P and anti-Jo-1 to cytoplasm. The study can give suggestions for further tests application and lab result checking.

Citation： WANGLi, RENLi-ling, GAOYu, LIWen, LIShuo, WUYong-kang. Analysis of the Relationship between Fluorescence Patterns of Antinuclear Antibody and Antinuclear Antibody Profiles. West China Medical Journal, 2015, 30(1): 84-88. doi: 10.7507/1002-0179.20150023 Copy

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11.	羅松, 梁前進, 王春梅, 等.重組表達的著絲粒蛋白-B N-端抗原在抗著絲粒自身免疫病血清檢測中的應用[J].解剖學報, 2004, 35(1):35-38.
12.	武永康, 王蘭蘭, 彭曉東, 等.抗著絲點抗體在自身免疫性疾病中的臨床應用價值探討[J].華西醫學, 2005, 20(4):650-652.
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14.	Almeida Gonzalez D, Casanas Rodriguez C, Magdalena Armas L, et al. Anti-ENA profiles related with anti-SS-A/Ro. The detection of Ro52 and Ro60 according to the presence of SS-B/La, and ANA pattern and titer[J]. Immunol Lett, 2014, 161(1):6-12.
15.	Li WX, Pan HF, Li LH, et al. Prevalence and clinical significance of 15 autoantibodies in patients with new-onset systemic lupus erythematosus[J]. Ir J Med Sci, 2010, 179(4):623-627.
16.	Hamaguchi Y, Fujimoto M, Matsushita T, et al. Common and distinct clinical features in adult patients with anti-aminoacyl-tRNA synthetase antibodies:heterogeneity within the syndrome[J]. PLoS One, 2013, 8(4):e60442.

1. Thomas L.臨床實驗診斷學:實驗結果的應用和評估[M].朱漢民, 沈霞, 呂元, 等, 譯.上海:上海科學技術出版社, 2004:794.
2. 郭春, 譚立明, 李洪文, 等. ENA檢測對診斷系統性紅斑狼瘡的臨床應用[J].中國實用醫藥, 2007, 2(31):118-119.
3. 彭曉東, 王蘭蘭, 張瑞薇, 等.抗核抗體熒光免疫檢測質量控制方法的建立和應用[J].中華檢驗醫學雜志, 2004, 27(3):190-192.
4. Kidd K, Cusi K, Mueller R, et al. Detection and identification of significant ANAs in previously determined ANA negative samples[J]. Clin Lab, 2005, 51(9/10):517-521.
5. 王莉, 任麗玲, 高宇, 等.抗Ro52抗體和抗Ro60抗體的臨床應用價值探討[J].華西醫學, 2014, 29(5):879-882.
6. Tani C, Carli L, Vagnani S, et al. The diagnosis and classification of mixed connective tissue disease[J]. J Autoimmun, 2014(48/49):46-49.
7. Wu Y, Cai B, Feng W, et al. Double positive CD4+CD8+T cells:key suppressive role in the production of autoantibodies in systemic lupus erythematosus[J]. Indian J Med Res, 2014, 140(4):513-519.
8. Ter Borgl EJ, Kelder JC. Lower prevalence of extra-glandular manifestations and anti-SSB antibodies in patients with primary Sjogren's syndrome and widespread pain:evidence for a relatively benign subset[J]. Clin Exp Rheumatol, 2014, 32(3):349-353.
9. Adelowo OO, Umeizudike T, Olaosebikan H, et al. Nephritis as an initial diagnosis of lupus in Nigerian patients[J]. Afr J Med Med Sci, 2014, 43(2):99-105.
10. Pasoto SG, Trindade Viana VS, Bonfa E. The clinical utility of anti-ribosomal P autoantibodies in systemic lupus erythematosus[J]. Expert Rev Clin Immunol, 2014, 10(11):1493-1503.
11. 羅松, 梁前進, 王春梅, 等.重組表達的著絲粒蛋白-B N-端抗原在抗著絲粒自身免疫病血清檢測中的應用[J].解剖學報, 2004, 35(1):35-38.
12. 武永康, 王蘭蘭, 彭曉東, 等.抗著絲點抗體在自身免疫性疾病中的臨床應用價值探討[J].華西醫學, 2005, 20(4):650-652.
13. Tonutti E, Bassetti D, Piazza A, et al. Diagnostic accuracy of Elisa methods as an alternative screening test to indirect immunofluorescence for the detection of antinuclear antibodies. Evaluation of five commercial kits[J]. Autoimmunity, 2004, 37(2):171-176.
14. Almeida Gonzalez D, Casanas Rodriguez C, Magdalena Armas L, et al. Anti-ENA profiles related with anti-SS-A/Ro. The detection of Ro52 and Ro60 according to the presence of SS-B/La, and ANA pattern and titer[J]. Immunol Lett, 2014, 161(1):6-12.
15. Li WX, Pan HF, Li LH, et al. Prevalence and clinical significance of 15 autoantibodies in patients with new-onset systemic lupus erythematosus[J]. Ir J Med Sci, 2010, 179(4):623-627.
16. Hamaguchi Y, Fujimoto M, Matsushita T, et al. Common and distinct clinical features in adult patients with anti-aminoacyl-tRNA synthetase antibodies:heterogeneity within the syndrome[J]. PLoS One, 2013, 8(4):e60442.

West China Medical Journal

Analysis of the Relationship between Fluorescence Patterns of Antinuclear Antibody and Antinuclear Antibody Profiles

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