Experimental Study of Intrahepatic Biliary Perfusion by Contrast Enhanced Ultrasonography via Bile Duct_West China Medical Journal

Authors：

 WANGHong ¹ , LINGWen-wu ¹ , MALin ¹ , CHENKe-fei ² ,  LUQiang ¹

1. Department of Ultrasound, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;
2. Department of General Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding?author：

LUQiang, Email: wsluqiang@126.com

Keywords：

Bile duct; Microbubble; Perfusion; Ultrasonic contrast

DOI：

10.7507/1002-0179.20150016

Video：

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Objective To explore the possibility of intrahepatic biliary perfusion by contrast enhanced ultrasonography via bile duct. Methods Sixteen rabbits weighing 2 000-2 500 g were studied. A 22 gauge catheter was inserted in the common bile duct by surgery. The rabbits were divided into 4 groups according to the concentration of contrast agent, including group A:1/100 of standard concentration; group B:1/200 of standard concentration; group C:1/400 of standard concentration; and group D:1/800 of standard concentration. We observed the filling status, presence of outflow of contrast enhanced signal and satisfactory enhanced time after the injection of contrast agent via the catheter in common bile duct. Results Five milliliter of contrast agent was injected in each rabbit. The number of homogeneous filling in group A, B, C and D was 4, 4, 3 and 0 respectively. The number of presence of outflow of contrast enhanced signal in group A, B, C and D was 4, 1, 0 and 0 respectively. The satisfactory enhanced time in group A, B, C and D was (340±29) s, (284±37) s, (82±8) s and 0 s respectively. There was no statistical difference in the satisfactory enhanced time between group A and B (P=0.06) while significant difference in the satisfactory enhanced time between group A and C (P < 0.01), and between group B and C (P < 0.01) was found. Conclusion It is possible to perfuse the intrahepatic biliary system by sonographic contrast agents via bile duct, and 1/200 of standard concentration is a proper concentration of contrast agent to achieve satisfactory imaging.

Citation： WANGHong, LINGWen-wu, MALin, CHENKe-fei, LUQiang. Experimental Study of Intrahepatic Biliary Perfusion by Contrast Enhanced Ultrasonography via Bile Duct. West China Medical Journal, 2015, 30(1): 60-62. doi: 10.7507/1002-0179.20150016 Copy

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15.	蔡麗萍, 王琳, 趙克立, 等.膽管超聲造影在肝內膽管置管引流術式選擇中的應用價值[J].中華臨床醫師雜志:電子版, 2011, 5(8):2476-2477.
16.	Mao R, Xu EJ, Li K, et al. Usefulness of contrast-enhanced ultrasound in the diagnosis of biliary leakage following T-tube removal[J]. J Clin Ultrasound, 2010, 38(1):38-40.
17.	Xu EJ, Zheng RQ, Su ZZ, et al. Intra-biliary contrast-enhanced ultrasound for evaluating biliary obstruction during percutaneous transhepatic biliary drainage:a preliminary study[J]. Eur J Radiol, 2012, 81(12):3846-3850.
18.	許爾蛟, 鄭榮琴, 李凱, 等.膽管內超聲造影在經皮經肝膽道引流術中的應用價值[J].中華醫學超聲雜志:電子版, 2011, 8(9):1937-1945.
19.	郭歡儀, 許爾蛟, 鄭榮琴, 等.術中膽道超聲造影評價肝移植供體膽管解剖結構:二維與三維超聲顯像的比較[J].中華醫學超聲雜志:電子版, 2010, 7(10):1699-1705.

1. Claudon M, Dietrich CF, Choi B, et al. Guidelines and good clinical practice recommendations for contrast enhancedultrasound (CEUS) in the liver-update 2012:a WFUMB-EFSUMB initiative incooperation with representatives of AFSUMB, AIUM, ASUM, FLAUS and ICUS[J]. Ultraschall Med, 2013, 34(1):11-29.
2. Claudon M, Cosgrove D, Albrecht T, et al. Guidelines and good clinical practice recommendations for contrast enhanced ultrasound (CEUS)-update 2008[J]. Ultraschall Med, 2008, 29(1):28-44.
3. 戴晴, 姜玉新.超聲造影的臨床應用[J].中國醫學科學院學報, 2008(1):1-4.
4. 周洋, 杜聯芳.超聲造影觀察肝移植術后缺血性膽管病變的肝臟血流灌注[J].中國醫學影像技術, 2010, 26(7):1273-1276.
5. 毛楓, 張興偉, 李超倫, 等.周圍型肝內膽管細胞癌:超聲造影與增強CT對照[J].中國醫學影像技術, 2013(9):1477-1480.
6. 毛楓, 李超倫, 黃備建, 等.肝內膽管細胞癌超聲造影表現[J].中華醫學超聲雜志:電子版, 2013, 10(5):364-367.
7. 楊潔, 張學蘭, 梅麗霞, 等.實時超聲造影對肝外膽管癌的診斷價值[J].臨床超聲醫學雜志, 2009, 11(10):689-691.
8. 徐輝雄, 劉廣健, 呂明德, 等.肝內膽管癌超聲造影的增強特點[J].中國超聲醫學雜志, 2006, 22(4):294-296.
9. Ren J, Lu MD, Zheng RQ, et al. Evaluation of the microcirculatory disturbance of biliary ischemia after liver transplantation with contrast-enhanced ultrasound:preliminary experience[J]. Liver Transpl, 2009, 15(12):1703-1708.
10. Meacock LM, Sellars ME, Sidhu PS. Evaluation of gallbladder and biliary duct disease using microbubble contrast-enhanced ultrasound[J]. Br J Radiol, 2010, 83(991):615-627.
11. Xu HX. Contrast-enhanced ultrasound in the biliary system:potential uses and indications[J]. World J Radiol, 2009, 1(1):37-44.
12. Xu HX, Lu MD, Liu LN, et al. Imaging features of intrahepatic biliary cystadenoma and cystadenocarcinoma on B-mode and contrast-enhanced ultrasound[J]. Ultraschall Med, 2012, 33(7):E241-E249.
13. Ren J, Zheng BW, Wang P, et al. Revealing impaired blood supply to the bile ducts on contrast-enhanced ultrasound:a novel diagnosis method to ischemic-type biliary lesions after orthotropic liver transplantation[J]. Ultrasound Med Biol, 2013, 39(5):753-760.
14. 張艷玲, 許爾蛟, 鄭榮琴, 等.經皮二維膽道超聲造影的臨床應用[J].中國醫學影像技術, 2011, 27(6):1205-1208.
15. 蔡麗萍, 王琳, 趙克立, 等.膽管超聲造影在肝內膽管置管引流術式選擇中的應用價值[J].中華臨床醫師雜志:電子版, 2011, 5(8):2476-2477.
16. Mao R, Xu EJ, Li K, et al. Usefulness of contrast-enhanced ultrasound in the diagnosis of biliary leakage following T-tube removal[J]. J Clin Ultrasound, 2010, 38(1):38-40.
17. Xu EJ, Zheng RQ, Su ZZ, et al. Intra-biliary contrast-enhanced ultrasound for evaluating biliary obstruction during percutaneous transhepatic biliary drainage:a preliminary study[J]. Eur J Radiol, 2012, 81(12):3846-3850.
18. 許爾蛟, 鄭榮琴, 李凱, 等.膽管內超聲造影在經皮經肝膽道引流術中的應用價值[J].中華醫學超聲雜志:電子版, 2011, 8(9):1937-1945.
19. 郭歡儀, 許爾蛟, 鄭榮琴, 等.術中膽道超聲造影評價肝移植供體膽管解剖結構:二維與三維超聲顯像的比較[J].中華醫學超聲雜志:電子版, 2010, 7(10):1699-1705.

West China Medical Journal

Experimental Study of Intrahepatic Biliary Perfusion by Contrast Enhanced Ultrasonography via Bile Duct

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