The Clinical Value of Anti-Ro52 and Anti-Ro60_West China Medical Journal

Authors：

 WANGLi ¹ , RENLi-lin ² , GAOYu ¹ , LIWen ¹ , LIShuo ³ , ZUOChuan ⁴ ,  WUYong-kang ⁵

1. Department of Gynecology and Obstetrics, 452 Hospital of PLA, Chengdu, Sichuan 610061, P. R. China;
2. Department of Geriatrics Neurology, the Fifth Hospital of Chengdu, Chengdu, Sichuan 611130, P. R. China;
3. Department of Nutrition, 452 Hospital of PLA, Chengdu, Sichuan 610061, P. R. China;
4. Department of Rheumatology;
5. Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, P. R. China;

Corresponding?author：

WUYong-kang, Email: vipwyk@163.com

Keywords：

Autoimmune disease; Anti-SSa antibody; Anti-Ro52; Anti-Ro60

DOI：

10.7507/1002-0179.20140267

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Objective To study the diagnosis value of anti-SSa (including anti-Ro52 and anti-Ro60). Methods Antibodies of ENA (including Sm, Ro52, Ro60, SSb, RNP, Scl-70, Jo-1 and Rib-P) from 23145 patients with positive antinuclear antibody (ANA) were retrospected from January 2009 to December 2013. The relationship between anti-Ro52, anti-Ro60 and other test results and the diagnosis or symptomatic information of patients was also analyzed. Results The anti-Ro60 positive rate was 35.19% (8 145/23 145), and the anti-Ro52 was 13.16% (3 046/23 145) in 23145 ANA positive cases (P<0.05). The positive percentage of anti-Ro60 was higher in anti-SSb, anti-RNP, anti-Sm and anti-Rib-P positive cases than anti-Ro52 (P<0.05); the results of anti-Ro52 negative and anti-Ro60 positive (Ro52^-Ro60⁺) had a higher percentage in autoimmune diseases, non-autoimmune disease and symptoms groups than anti-Ro52 positive and anti-Ro60 negative (Ro52⁺Ro60^-) results (P<0.05). Conclusion The anti-Ro60 has higher positive rate than anti-Ro52, and the sensitivity and prediction value of autoimmune diseases of anti-Ro60 are better than anti-Ro52. But both anti-Ro60 and anti-Ro52 have poor specificity for disease diagnosis.

Citation： WANGLi, RENLi-lin, GAOYu, LIWen, LIShuo, ZUOChuan, WUYong-kang. The Clinical Value of Anti-Ro52 and Anti-Ro60. West China Medical Journal, 2014, 29(5): 879-882. doi: 10.7507/1002-0179.20140267 Copy

1.	Aframian DJ, Konttinen YT, Carrozzo M, et al. Urban legends series:Sj?gren's syndrome[J]. Oral Dis, 2013, 19(1):46-58.
2.	Morozzi G, Bellisai F, Simpatico A, et al. Comparison of different methods for the detection of anti-Ro/SSA antibodies in connective tissue diseases[J]. Clin Exp Rheumatol, 2001, 18(6):729-731.
3.	蔡逸婷, 劉慶中, 趙超, 等. 聯合檢測抗α胞襯蛋白抗SSA和抗SSB抗體在干燥綜合征診斷中的應用[J]. 現代免疫學, 2012, 32(2):152-155.
4.	Tan EM. Antinuclear antibodies:diagnostic markers for autoimmune diseases and probes for cell biology[J]. Adv Immunol, 1989(44):93-151.
5.	Kobak S, Oksel F, Aksu K, et al. The frequency of sicca symptoms and Sj?gren's syndrome in patients with systemic sclerosis[J]. Int J Rheum Dis, 2013, 16(1):88-92.
6.	Shiboski SC, Shiboski CH, Criswell L, et al. American College of Rheumatology classification criteria for Sj?gren's syndrome:a data-driven, expert consensus approach in the Sj?gren's International Collaborative Clinical Alliance cohort[J]. Arthritis Care Res (Hoboken), 2012, 64(4):475-487.
7.	石書梅, 趙學智. 混合性結締組織病致繼發性膜性腎病1例并文獻復習[J]. 中國中西醫結合腎病雜志, 2011, 12(8):719-720.
8.	Adelowo OO, Ojo O, Oduenyi I. Auto antibodies in Nigerian lupus patients[J]. Afr J Med Med Sci, 2012, 41(2):177-181.
9.	Asero R, D'agostino P, Bertetti E, et al. Clinical findings in patients with SLE whose sera contain antibodies to ribosomal ribonucleoprotein[J]. Immunol Lett, 1988, 18(1):1-3.
10.	Retamozo S, Akasbi M, Brito-Zerón P, et al. Anti-Ro52 antibody testing influences the classification and clinical characterisation of primary Sj?gren's syndrome[J]. Clin Exp Rheumatol, 2012, 30(5):686-692.
11.	Bizzaro N, Bonelli F, Tonutti E, et al. New coupled-particle light-scattering assay for detection of Ro/SSA (52 and 60 kilodaltons) and La/SSB autoantibodies in connective tissue diseases[J]. Clin Diagn Lab Immunol, 2001, 8(5):922-925.
12.	Menéndez A, Gómez J, Escanlar E, et al. Clinical associations of anti-SSA/Ro60 and anti-Ro52/TRIM21 antibodies:diagnostic utility of their separate detection[J]. Autoimmunity, 2013, 46(1):32-39.
13.	de Zubiria Salgado A, Herrera-Diaz C. Lupus nephritis:an overview of recent findings[J/OL]. Autoimmune Dis, 2012. http://dx.doi.org/10.1155/2012/849684.
14.	Langguth DM, Morris S, Clifford L, et al. Specific testing for "isolated" anti-52 kDa SSA/Ro antibodies during standard anti-extractable nuclear antigen testing is of limited clinical value[J]. J Clin Pathol, 2007, 60(6):670-673.
15.	黃清水, 胡添, 羅忠勤, 等. 856例抗Ro52抗體陽性住院患者回顧性分析[J]. 臨床檢驗雜志, 2013, 31(9):709.

1. Aframian DJ, Konttinen YT, Carrozzo M, et al. Urban legends series:Sj?gren's syndrome[J]. Oral Dis, 2013, 19(1):46-58.
2. Morozzi G, Bellisai F, Simpatico A, et al. Comparison of different methods for the detection of anti-Ro/SSA antibodies in connective tissue diseases[J]. Clin Exp Rheumatol, 2001, 18(6):729-731.
3. 蔡逸婷, 劉慶中, 趙超, 等. 聯合檢測抗α胞襯蛋白抗SSA和抗SSB抗體在干燥綜合征診斷中的應用[J]. 現代免疫學, 2012, 32(2):152-155.
4. Tan EM. Antinuclear antibodies:diagnostic markers for autoimmune diseases and probes for cell biology[J]. Adv Immunol, 1989(44):93-151.
5. Kobak S, Oksel F, Aksu K, et al. The frequency of sicca symptoms and Sj?gren's syndrome in patients with systemic sclerosis[J]. Int J Rheum Dis, 2013, 16(1):88-92.
6. Shiboski SC, Shiboski CH, Criswell L, et al. American College of Rheumatology classification criteria for Sj?gren's syndrome:a data-driven, expert consensus approach in the Sj?gren's International Collaborative Clinical Alliance cohort[J]. Arthritis Care Res (Hoboken), 2012, 64(4):475-487.
7. 石書梅, 趙學智. 混合性結締組織病致繼發性膜性腎病1例并文獻復習[J]. 中國中西醫結合腎病雜志, 2011, 12(8):719-720.
8. Adelowo OO, Ojo O, Oduenyi I. Auto antibodies in Nigerian lupus patients[J]. Afr J Med Med Sci, 2012, 41(2):177-181.
9. Asero R, D'agostino P, Bertetti E, et al. Clinical findings in patients with SLE whose sera contain antibodies to ribosomal ribonucleoprotein[J]. Immunol Lett, 1988, 18(1):1-3.
10. Retamozo S, Akasbi M, Brito-Zerón P, et al. Anti-Ro52 antibody testing influences the classification and clinical characterisation of primary Sj?gren's syndrome[J]. Clin Exp Rheumatol, 2012, 30(5):686-692.
11. Bizzaro N, Bonelli F, Tonutti E, et al. New coupled-particle light-scattering assay for detection of Ro/SSA (52 and 60 kilodaltons) and La/SSB autoantibodies in connective tissue diseases[J]. Clin Diagn Lab Immunol, 2001, 8(5):922-925.
12. Menéndez A, Gómez J, Escanlar E, et al. Clinical associations of anti-SSA/Ro60 and anti-Ro52/TRIM21 antibodies:diagnostic utility of their separate detection[J]. Autoimmunity, 2013, 46(1):32-39.
13. de Zubiria Salgado A, Herrera-Diaz C. Lupus nephritis:an overview of recent findings[J/OL]. Autoimmune Dis, 2012. http://dx.doi.org/10.1155/2012/849684.
14. Langguth DM, Morris S, Clifford L, et al. Specific testing for "isolated" anti-52 kDa SSA/Ro antibodies during standard anti-extractable nuclear antigen testing is of limited clinical value[J]. J Clin Pathol, 2007, 60(6):670-673.
15. 黃清水, 胡添, 羅忠勤, 等. 856例抗Ro52抗體陽性住院患者回顧性分析[J]. 臨床檢驗雜志, 2013, 31(9):709.

West China Medical Journal

The Clinical Value of Anti-Ro52 and Anti-Ro60

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